Home About us Contact
|
Home About us Contact | ||
|
|
||
Wall Degradation (wall + degradation)
Selected AbstractsModification of impact craters in the northern plains of Mars: Implications for Amazonian climate historyMETEORITICS & PLANETARY SCIENCE, Issue 10 2006M. A. Kreslavsky Two of the 130 craters have unusually rough ejecta; they are deep, have steep walls, and are apparently the youngest in the population. Icy mantles filling the local subkilometer-scale topographic lows is the main contribution to ejecta smoothing, which occurs at a time scale on the order of tens of Myr. Wall degradation and crater shallowing generally occur at longer time scales, comparable to the duration of the Amazonian period. Many craters are shallow due to filling of the crater with specific ice-rich material of uncertain origin. We use our collected data to infer the nature of the past climate back through the Amazonian, a period prior to ,10,20 Myr ago, when orbital parameter solutions are chaotic and one must rely on geological data to infer climate conditions. We conclude that moderately high obliquity and wide obliquity variations were probable during the last 40,160 Myr. We tentatively conclude that high obliquity peaks (>40,45°) may have occurred episodically through the last 210,430 Myr. A sharp step in the frequency distribution of wall steepness at 20° may indicate a geologically long period prior to that time where obliquity never exceeded 40,45°. [source] Splitting of the fission yeast septumFEMS YEAST RESEARCH, Issue 6 2007Matthias Sipiczki Abstract In cell-walled organisms, a cross wall (septum) is produced during cytokinesis, which then splits in certain organisms to allow the daughter cells to separate. The formation and the subsequent cleavage of the septum require wall synthesis and wall degradation, which need to be strictly coordinated in order to prevent cell lysis. The dividing fission yeast (Schizosaccharomyces) cell produces a three-layered septum in which the middle layer and a narrow band of the adjacent cell wall can be degraded without threatening the integrity of the separating daughter cells. This spatially very precise process requires the activity of the Agn1p 1,3-,-glucanase and the Eng1p 1,3-,-glucanase, which are localized to the septum by a complex mechanism involving the formation of a septin ring and the directed activity of the exocyst system. The Sep1p-Ace2p transcription-factor cascade regulates the expression of many genes producing proteins for this complex process. Recent advances in research into the molecular mechanisms of separation and its regulation are discussed in this review. [source] In vitro fermentative characteristics of ruminant diets supplemented with fibrolytic enzymes and ranges of optimal endo-,-1,4-glucanase activityJOURNAL OF ANIMAL PHYSIOLOGY AND NUTRITION, Issue 2 2010E. González-García Summary Effectiveness of fibrolytic enzymes supplementing a range of forage to concentrate (F:C) diets was assessed with goat (G) or cow (C) inoculum using the gas production (GP) technique. Four F:C diets were evaluated: forage (1:0), high forage (0.7:0.3), medium forage (0.5:0.5) and low forage (0.3:0.7) diets, supplemented or not with PromoteTM (PRO) at 1 or 2 ml/kg dry matter (DM). The GP kinetic was different between F:C (1:0 < 0.7:0.3 < 0.5:0.5 < 0.3:0.7) and inoculum. Responses to enzyme were positively related to forage level and differed with inoculum. The neutral detergent fibre and acid detergent fibre degradation were depressed by the concentrate in the substrates fermented with C and were not altered or even enhanced in G sets. Results confirm that increasing starch proportion modified the pattern of microbial fermentation, while no influences were detected in the improvement of cell wall degradation with fibrolytic enzymes. Another in vitro experiment was conducted to investigate factors by which endo-,-1,4-glucanase activity (EA) of PRO is compromised in a factorial design (3 × 4 × 3) for three pH (4.0, 5.5 and 6.5), four temperatures (30, 40, 50 and 70 °C) and three doses (1, 2 and 3 ml/kg DM of substrate). Maximum EA were obtained for pH 4.0, 50 °C and 3 ml/kg DM. Optimal conditions for PRO proved to be outside the normal ranges in ruminal environment. [source] The SUN41 and SUN42 genes are essential for cell separation in Candida albicansMOLECULAR MICROBIOLOGY, Issue 5 2007Arnaud Firon Summary Completion of the yeast cell cycle involves extensive remodelling of the cell wall upon separation of mother and daughter cells. We have studied two members of the ascomycete-specific SUN gene family in Candida albicans. Inactivation of SUN41 yields defects in cell separation and hyphal elongation while inactivation of SUN42 results in minor phenotypic alterations. Simultaneous inactivation of SUN41 and SUN42 is synthetically lethal due to lysis of mother cells after septation. Electronic microscopy reveals cell wall defects mainly localized in the region surrounding the septa. This phenotype is osmoremediable and the conditional double mutants show increased sensitivity to cell wall or cell membrane perturbing agents. The essential function shared by Sun41p and Sun42p is conserved among yeasts because UTH1, a Saccharomyces cerevisiae SUN gene, suppresses the lethality of SUN41 and SUN42 conditional mutants. Investigation of functional genomic data obtained in S. cerevisiae reveals links between members of the SUN gene family and the RAM pathway regulating cell wall-degrading enzymes specifically involved during cell separation. Thus, the main function of ascomycetous Sun proteins appears linked to cell wall remodelling, with a probable role in counter-balancing cell wall degradation to avoid cell lysis upon cell separation. [source] Involvement of hydrogen peroxide in leaf abscission signaling, revealed by analysis with an in vitro abscission system in Capsicum plantsTHE PLANT JOURNAL, Issue 1 2008Masaru Sakamoto Summary Although auxin and ethylene play pivotal roles in leaf abscission, the subsequent signaling molecules are poorly understood. This is mainly because it is difficult to effectively treat the intact abscission zone (AZ) with pharmacological reagents. We developed an in vitro experimental system that reproduces stress-induced leaf abscission in planta. In this system, 1-mm-thick petiole strips, encompassing the AZ, were separated within 4 days of abscission at the AZ through cell wall degradation in an auxin depletion- and ethylene-dependent manner. The system allowed us to show that hydrogen peroxide (H2O2) is involved in abscission signaling. Microscopic analyses revealed continuous H2O2 production by AZ cells. H2O2 scavengers and diphenylene iodonium, an inhibitor of NADPH oxidase, suppressed in vitro abscission and cellulase expression. Conversely, the application of H2O2 promoted in vitro abscission and expression of cellulase. Ethephon-induced abscission was suppressed by inhibitors of H2O2 production, whereas the expression of ethylene-responsive genes was unaffected by both H2O2 and an H2O2 inhibitor. These results indicated that H2O2 acts downstream from ethylene in in vitro abscission signaling. In planta, salinity stress induced the expression of genes that respond to ethylene and reactive oxygen species, and also induced H2O2 production at the AZ, which preceded leaf abscission. These results indicate that H2O2 has roles in leaf abscission associated with ethylene both in vitro and in planta. [source] | ||||||||||