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Wall Consisting (wall + consisting)
Selected AbstractsObservations of Dermocystidium sp. infections in bullheads, Cottus gobio L., from a river in southern EnglandJOURNAL OF FISH DISEASES, Issue 4 2004S W Feist Abstract Bullheads, Cottus gobio, with macroscopic external cysts on the skin and fins measuring up to 3 mm in diameter were detected in the River Allen and its tributaries in southern England between 1992 and 1998. The prevalence of these cysts was up to 50% at some sites. Examination of cyst contents revealed the presence of numerous spores, typical of the genus Dermocystidium, measuring 8 ,m in diameter. The parasite developed within well-defined cysts, which were located in the hypodermal connective tissues of the host. No cysts were present on the fins of any of the fish examined. Histological examination revealed a cyst wall consisting of an inner layer of dense eosinophilic material similar to that reported for Dermocystidium spp. forming coenocytic hyphae. No evidence was found of systemic infection or hyphal formation. Spores contained a prominent refractile body, which gave a weakly positive reaction for polysaccharides with the periodic-acid Schiff reaction and was positively stained with acidic dyes. Several examples of ruptured cysts were seen in histological sections and in some of these cases the host epithelial layer was breached, allowing release of the spores to the environment. Morphological features of, and host response towards, the Dermocystidium sp. in bullheads are compared with similar infections in salmonids and other freshwater fish species. [source] Identification of pseudomurein cell wall binding domainsMOLECULAR MICROBIOLOGY, Issue 6 2006Peter J. M. Steenbakkers Summary Methanothermobacter thermautotrophicus is a methanogenic Gram-positive microorganism with a cell wall consisting of pseudomurein. Currently, no information is available on extracellular pseudomurein biology and so far only two prophage pseudomurein autolysins, PeiW and PeiP, have been reported. In this paper we show that PeiW and PeiP contain two different N-terminal pseudomurein cell wall binding domains. This finding was used to identify a novel domain, PB007923, on the M. thermautotrophicus genome present in 10 predicted open reading frames. Three homologues were identified in the Methanosphaera stadtmanae genome. Binding studies of fusion constructs of three separate PB007923 domains to green fluorescent protein revealed that it also constituted a cell wall binding domain. Both prophage domains and the PB007923 domain bound to the cell walls of Methanothermobacter species and fluorescence microscopy showed a preference for the septal region. Domain specificities were revealed by binding studies with other pseudomurein-containing archaea. Localized binding was observed for M. stadtmanae and Methanobrevibacter species, while others stained evenly. The identification of the first pseudomurein cell wall binding domains reveals the dynamics of the pseudomurein cell wall and provides marker proteins to study the extracellular pseudomurein biology of M. thermautotrophicus and of other pseudomurein-containing archaea. [source] Isolation and proteomic alalysis of cell wall-deficient Haematococcus pluvialis mutantsPROTEINS: STRUCTURE, FUNCTION AND BIOINFORMATICS, Issue 18 2005Sheng-Bing Wang Abstract The green alga Haematococcus pluvialis has a plant-like cell wall consisting of glycoproteins and cellulose that is modified during the cell cycle and under various conditions. These features allow Haematococcus to be used as a model organism for studying cell wall biology. Development of the Haematococcus model is hampered by the absence of mutants that could provide insight into the biosynthesis and assembly of wall components. Haematococcus mutants (WM#537 and WM#2978) (WM#wall mutant) with defective cell walls were obtained by chemical mutagenesis. WM#537 features a secondary wall of considerably reduced thickness, whereas WM#2978 possesses a somewhat reduced secondary wall with little intervening space between the wall and plasmalemma. 2-DE revealed that a majority of the cell wall proteins were present in the wild-type and mutant cell walls throughout the cell cycle. PMF identified 55 wall protein orthologs from these strains, including a subset of induced proteins known to be involved in wall construction, remodeling, and defense. Down-regulation of certain wall proteins in the two mutants was associated with the wall defects, whereas overexpression of other proteins may have compensated for the defective walls in the two mutants. [source] Seamount volcanism along the Gakkel Ridge, Arctic OceanGEOPHYSICAL JOURNAL INTERNATIONAL, Issue 3 2008James R. Cochran SUMMARY The Gakkel Ridge in the Arctic Ocean is the slowest spreading portion of the global mid-ocean ridge system. Total spreading rates vary from 12.8 mm yr,1 near Greenland to 6.5 mm yr,1 at the Siberian margin. Melting models predict a dramatic decrease in magma production and resulting crustal thickness at these low spreading rates. At slow spreading ridges, small volcanic seamounts are a dominant morphologic feature of the rift valley floor and an important mechanism in building the oceanic crust. This study quantitatively investigates the extent, nature and distribution of seamount volcanism at the ultraslow Gakkel Ridge, the manner in which it varies along the ridge axis and the relationship of the volcanoes to the larger scale rift morphology. A numerical algorithm is used to identify and characterize isolated volcanic edifices by searching gridded swath-bathymetry data for closed concentric contours protruding above the surrounding seafloor. A maximum likelihood model is used to estimate the total number of seamounts and the characteristic height within different seamount populations. Both the number and size of constructional volcanic features is greatly reduced at the Gakkel Ridge compared with the Mid-Atlantic Ridge (MAR). The density of seamounts (number/area) on the rift valley floor of the Western Volcanic Zone (WVZ) is ,55% that of the MAR. The observed volcanoes are also much smaller, so, the amount of erupted material is greatly reduced compared with the MAR. However, the WVZ is still able to maintain a MAR-like morphology with axial volcanic ridges, volcanoes scattered on the valley floor and rift valley walls consisting of high-angle faults. Seamount density at the Eastern Volcanic Zone (EVZ) is ,45% that of the WVZ (,25% that of the MAR). Seamounts are clustered at the widely spaced magmatic centres characteristic of the EVZ, although some seamounts are found between magmatic centres. These seamounts tend to be located at the edge of the rift valley or on the valley walls rather than on the valley floor. Seamounts in the Sparsely Magmatic Zone (SMZ) are located almost entirely at the 19°E magmatic centre with none observed within a 185 km-long portion of the rift valley floor. The EVZ and SMZ appear to display a mode of crustal accretion, characterized by extreme focusing of melt to the magmatic centres. Magmas erupted between the magmatic centres appear to have ascended along faults. This is very different from what is observed at the WVZ (or the MAR), and there is a threshold transition between the two modes of crustal accretion. At the Gakkel Ridge, the location of the transition appears to be localized by a boundary in mantle composition. [source] Interfacial living radical copolymerization of oil- and water-soluble comonomers to form composite polymer capsulesJOURNAL OF POLYMER SCIENCE (IN TWO SECTIONS), Issue 1 2006Mir Mukkaram Ali Abstract The suspension copolymerization of methyl methacrylate with hydroxy-functional poly(ethylene glycol) monomethacrylate (PEGMA) by atom transfer radical polymerization (ATRP) yielded soluble, controlled-molecular-weight amphiphilic copolymers (weight-average molecular weight/number-average molecular weight <1.3). Despite extensive partitioning of PEGMA into the water phase, copolymers containing up to 24 mol % PEGMA were formed in the oil phase, from comonomer feeds containing 30 mol % PEGMA. Conversions by suspension polymerization were comparable to those obtained by solution polymerization, at over 70%. Suspension copolymers with high PEGMA contents contained high-molecular-weight polymer formed by uncontrolled polymerization, unless poly(vinyl pyrrolidone) was added to displace the growing polymer from the interface. The addition of diethylene glycol dimethacrylate gave capsules at 17 mol % PEGMA with ATRP, whereas conventional free-radical polymerization required 24 mol % PEGMA to form capsules. The lower PEGMA level required for capsule formation with ATRP was attributed to the lower rates of propagation and crosslinking and to improved incorporation of PEGMA into the final gels. Suspension ATRP with 24 mol % PEGMA in the feed gave two-layer capsule walls consisting of an inner layer visible by transmission electron microscopy and an outer layer visible by both transmission electron microscopy and environmental scanning electron microscopy, which indicated a compositional gradient across the capsule wall. © 2005 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 44: 156,171, 2006 [source] | ||||||||||