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Wall Composition (wall + composition)

Distribution by Scientific Domains
Life Sciences57%

Kinds of Wall Composition

  • cell wall composition
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    Selected Abstracts

    Ultrastructure, Eneystment and Cyst Wall Composition of the Resting Cyst of the Peritrich Ciliate Opisthonecta henneguyi

    THE JOURNAL OF EUKARYOTIC MICROBIOLOGY, Issue 1 2003
    PURIFICACIÓN CALVO
    ABSTRACT. The cyst wall of Opisthonecta henneguyi has been studied ultrastructurally and cytochemically by light and electron microscopy, as well as by chemical and electrophoretic analyses, to examine the structure of the cyst wall and its composition. The cyst wall consists of four morphologically distinct layers. The ectocyst is a thin dense layer. The mesocyst is the thickest layer and is composed of a compact material. The endocyst is a thin layer like the ectocyst, but less dense. The granular layer varies in thickness and is composed of a granular material. In the resting cyst, kinetosomes of both oral apparatus and trochal band as well as the myoneme system are maintained, and only cilia are resorbed. The sugars present in the cyst wall are predominantly N-acetylglucosamine (90%) and glucose (10%). The niesocyst is composed of chitin, and the endocyst includes glycoproteins and acid mucopolysaccharides. During secretion of the cyst wall, the endocyst and granular layer are secreted from precursors synthesized "de novo". No cytoplasmic precursors of ectocyst and mesocyst have been detected. [source]

    Absence of Gup1p in Saccharomyces cerevisiae results in defective cell wall composition, assembly, stability and morphology

    FEMS YEAST RESEARCH, Issue 7 2006
    Célia Ferreira
    Abstract Saccharomyces cerevisiae Gup1p and its homologue Gup2p, members of the superfamily of membrane-bound O -acyl transferases, were previously associated with glycerol-mediated salt-stress recovery and glycerol symporter activity. Several other phenotypes suggested Gup1p involvement in processes connected with cell structure organization and biogenesis. The gup1, mutant is also thermosensitive and exhibits an altered plasma membrane lipid composition. The present work shows that the thermosensitivity is independent of glycerol production and retention. Furthermore, the mutant grows poorly on salt, ethanol and weak carboxylic acids, suggestive of a malfunctioning membrane potential. Additionally, gup1, is sensitive to cell wall-perturbing agents, such as Calcofluor white, Zymolyase, lyticase and sodium dodecyl sulphate and exhibits a sedimentation/aggregation phenotype. Quantitative analysis of cell wall components yielded increased contents of chitin and ,-1,3-glucans and lower amounts of mannoproteins. Consistently, scanning electron microscopy showed a strikingly rough surface morphology of the mutant cells. These results suggest that the gup1, is affected in cell wall assembly and stability, although the Slt2p/MAP kinase from the PKC pathway was phosphorylated during hypo-osmotic shock to a normal extent. Results emphasize the pleiotropic nature of gup1,, and are consistent with a role of Gulp1p in connection with several pathways for cell maintenance and construction/remodelling. [source]

    Analysis of adobe wall composition at the Chaves-Hummingbird Site, New Mexico, by diffuse reflectance spectrophotometry

    GEOARCHAEOLOGY: AN INTERNATIONAL JOURNAL, Issue 8 2007
    William Balsam
    This article investigates adobe wall construction materials utilized by prehistoric inhabitants of Chaves-Hummingbird Pueblo, an ancestral Pueblo village located ,20 miles west of Albuquerque, New Mexico. The walls were constructed with native clay-rich soils some time between approximately 1275,1450 A.D. Samples were analyzed with a diffuse reflectance spectrophotometer from the near ultraviolet (NUV) through the visible (VIS) and into the near infrared (NIR) region of the electromagnetic spectrum. Cluster analysis of samples from 275 adobe walls and 36 soil locations surrounding the pueblo room blocks indicates four clusters. Comparison of typical samples from the four clusters indicates that they are very similar and are distinguished by minor variations in the three primary spectrally determined components, Na-Ca montmorillonite, bentonite, and goethite. In general, clusters correspond with room construction episodes that are discernible through patterns of wall bonding and abutment recorded during the archaeological investigation of the site. This suggests that during different phases of construction the source of the wall adobe changed. Many of the soil samples are included in wall clusters and therefore reveal a potential source of material used for adobe, adjacent soils. However, not all the soil surrounding the pueblo grouped with wall clusters indicating a preference for certain soil types and that some soils were probably unsuitable for making adobe. Therefore, diversity in spectrally identified construction materials provides insights into source locations and possible construction preferences of the site inhabitants. © 2007 Wiley Periodicals, Inc. [source]

    Cell wall composition of vascular and parenchyma tissues in broccoli stems

    JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 13 2003
    S Müller
    Abstract Broccoli stems can become tough and stringy owing to excessive development of the vascular ring. Thickened cell walls from the vascular ring were isolated and their composition was determined. They were derived principally from anatomically recognisable xylem vessels, fibres and tracheids but contained an assemblage of polysaccharides typical of primary cell walls. Their pectin content was particularly high and they contained only 6% lignin as estimated by solid state 13C NMR spectroscopy. They did not differ markedly in composition from parenchyma cell walls within the same stems. Thus, despite their thickness and anatomical appearance, these cell walls resembled the walls of non-woody cells in their polymer composition. Copyright © 2003 Society of Chemical Industry [source]

    Proboscis morphology and food preferences in nymphalid butterflies (Lepidoptera: Nymphalidae)

    JOURNAL OF ZOOLOGY, Issue 1 2001
    H. W. Krenn
    Abstract Many species of nymphalid butterflies only exceptionally visit flowers and feed instead on tree sap, juice of rotting fruits and other decaying substances. To investigate whether the proboscis morphology of these non-flower-visiting Nymphalidae differs from that of nectarivorous butterflies, representatives from 64 nymphalid species with known feeding preferences were examined. Morphometric comparison of the proboscis revealed characteristic differences in proboscis length, tip-region length, wall composition, and number and shape of proboscis sensilla between these two feeding guilds. The investigated non-flower-visiting species belonging to Apaturinae, Limenitidinae, Morphinae, Brassolinae, Nymphalinae and Satyrinae, possess a relatively short and light-coloured proboscis which has a long tip-region with a great number of club-shaped sensilla styloconica. Densely arranged, these sensilla form a flat brush located laterally from the openings into the food canal on the dorsal side of the tip-region. Among the non-flower-visiting species, a second type of proboscis was found in fruit-feeding Charaxinae the stout tip-region of which is equipped with more widely spaced sensilla styloconica. The investigated flower-visiting Heliconiinae, Nymphalinae, Satyrinae, Danainae and Ithomiinae are characterized by a slender, darker-coloured proboscis with a rather short tip-region bearing fewer sensilla styloconica in a loose arrangement. Discriminant analysis revealed that the tip-region length, the number of sensilla styloconica and the relative proboscis length are the most important discriminating variables between the flower-visiting and the non-flower-visiting species. The proboscis morphology of nymphalid butterflies corresponds with certain feeding habits and allow us to make predictions on their food preferences. The ,brush-tipped' proboscis seems to have a functional role in the accumulation of fluid and the uptake of liquid from wet surfaces such as rotting fruits or tree sap. We conclude from the phylogeny of the examined taxa that this derived proboscis tip morphology evolved several times independently as an adaptation to the exploitation of new food resources. [source]

    PMT family of Candida albicans: five protein mannosyltransferase isoforms affect growth, morphogenesis and antifungal resistance

    MOLECULAR MICROBIOLOGY, Issue 2 2005
    Stephan K.-H.
    Summary Protein O -mannosyltransferases (Pmt proteins) initiate O- mannosylation of secretory proteins. The PMT gene family of the human fungal pathogen Candida albicans consists of PMT1 and PMT6, as well as three additional PMT genes encoding Pmt2, Pmt4 and Pmt5 isoforms described here. Both PMT2 alleles could not be deleted and growth of conditional strains, containing PMT2 controlled by the MET3- or tetOScHOP1- promoters, was blocked in non-permissive conditions, indicating that PMT2 is essential for growth. A homozygous pmt4 mutant was viable, but synthetic lethality of pmt4 was observed in combination with pmt1 mutations. Hyphal morphogenesis of a pmt4 mutant was defective under aerobic induction conditions, yet increased in embedded or hypoxic conditions, suggesting a role of Pmt4p-mediated O- glycosylation for environment-specific morphogenetic signalling. Although a PMT5 transcript was detected, a homozygous pmt5 mutant was phenotypically silent. All other pmt mutants showed variable degrees of supersensitivity to antifungals and to cell wall-destabilizing agents. Cell wall composition was markedly affected in pmt1 and pmt4 mutants, showing a significant decrease in wall mannoproteins. In a mouse model of haematogenously disseminated infection, PMT4 was required for full virulence of C. albicans. Functional analysis of the first complete PMT gene family in a fungal pathogen indicates that Pmt isoforms have variable and specific roles for in vitro and in vivo growth, morphogenesis and antifungal resistance. [source]

    Modified immunohistological staining allows detection of Ziehl,Neelsen-negative Mycobacterium tuberculosis organisms and their precise localization in human tissue

    THE JOURNAL OF PATHOLOGY, Issue 5 2005
    Timo Ulrichs
    Abstract The diagnosis of mycobacterial infection depends on the Ziehl,Neelsen (ZN) stain, which detects mycobacteria because of their characteristic acid-fast cell wall composition and structure. The histological diagnosis of tuberculosis (TB) comprises various aspects: (1) sensitive detection of mycobacteria; (2) precise localization of mycobacteria in the context of granulomatous lesions; (3) ,staging' of disease according to mycobacterial spread and granulomatous tissue integrity. Thus, detection of minute numbers of acid-fast bacteria in tissue specimens is critical. The conventional ZN stain fails to identify mycobacteria in numbers less than 104 per ml. Hence many infections evade diagnosis. PCR is highly sensitive, but allows neither localization within tissues nor staging of mycobacterial disease, and positive findings frequently do not correlate with disease. In this study, an anti- Mycobacterium bovis bacille Calmette,Guérin polyclonal antiserum (pAbBCG) was used to improve immunostaining, which was compared to the ZN stain in histological samples. Screening of tissue samples including lungs, pleural lesions, lymph nodes, bone marrow, and skin for mycobacterial infection revealed that pAbBCG staining detects infected macrophages harbouring intracellular mycobacteria or mycobacterial material as well as free mycobacteria that are present at low abundance and not detected by the ZN stain. The positive pAbBCG staining results were confirmed either by PCR analysis of microdissected stained tissue or by culture from tissue. This immunostaining approach allows precise localization of the pathogen in infected tissue. Copyright © 2005 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. [source]