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Kinds of WA Selected AbstractsNON-GYNAECOLOGICAL CYTOLOGY: THE CLINICIAN'S VIEWCYTOPATHOLOGY, Issue 2006I. Penman There is increased recognition of the importance of accurate staging of malignancies of the GI tract and lung, greater use of neoadjuvant therapies and more protocol-driven management. This is particularly important where regional lymph node involvement significantly impacts on curability. Multidetector CT and PET scanning have resulted in greater detection of potential abnormalities which, if positive for malignancy, would change management. There is also a greater recognition that many enlarged nodes may be inflammatory and that size criteria alone are unreliable in determining involvement. In other situations, especially pancreatic masses, not all represent carcinoma as focal chronic pancreatitis, autoimmune pancreatitis etc can catch out the unwary. A preoperative tissue diagnosis is essential and even if unresectable, oncologists are increasingly reluctant to initiate chemotherapy or enroll patients into trials without this. The approach to obtaining tissue is often hampered by the small size or relative inaccessibility of lesions by percutaneous approaches. As such novel techniques such as endoscopic ultrasound (EUS) guided FNA have been developed. A 120cm needle is passed through the instrument and, under real-time visualisation, through the gastrointestinal wall to sample adjacent lymph nodes or masses. Multiple studies have demonstrated the safety and performance of this technique. In oesophageal cancer, confirmation of node positivity by has a major negative influence on curative resection rates and will often lead to a decision to use neoadjuvant chemotherapy or a non-operative approach. Sampling of lymph nodes at the true coeliac axis upstages the patient to M1a status (stage IV) disease and makes the patient incurable. In NSCLC, subcarinal lymph nodes are frequently present but may be inflammatory. If positive these represent N2 (stage IIIA) disease and in most centres again makes the patient inoperable. Access to these lymph nodes would otherwise require mediastinosocopy whereas this can be done simply, safely and quickly by EUS. Overall the sensitivity for EUS , FNA of mediastinal or upper abdominal lymph nodes is 83,90% with an accuracy of 80,90%. In pancreatic cancer performance is less good but pooled analysis of published studies indicates a sensitivity of 85% and accuracy of 88%. In a recent spin-off from EUS, endobronchial ultrasound (EBUS) instruments have been developed and the ability to sample anterior mediastinal nodes has been demonstrated. It is likely that this EBUS , FNA technique will become increasingly utilised and may replace mediastinoscopy. The development of techniques such as EUS and EBUS to allow FNA sampling of lesions has increased the role of non-gynaecological cytology significantly in recent years. Cytology therefore remains important for a broad range of specialties and there is ongoing need for careful and close co-operation between cytologists and clinicians in these specialties. References:, 1. Williams DB, Sahai AV, Aabakken L, Penman ID, van Velse A, Webb J et al. Endoscopic ultrasound guided fine needle aspiration biopsy: a large single centre experience. Gut. 1999; 44: 720,6. 2. Silvestri GA, Hoffman BJ, Bhutani MS et al. Endoscopic ultrasound with fine-needle aspiration in the diagnosis and staging of lung cancer. Ann Thorac Surg 1996; 61: 1441,6. 3. Rintoul RC, Skwarski KM, Murchison JT, Wallace WA, Walker WS, Penman ID. Endobronchial and endoscopic ultrasound real-time fine-needle aspiration staging of the mediastinum ). Eur Resp J 2005; 25: 1,6. [source] Prevalence and predictors of recurrence of major depressive disorder in the adult populationACTA PSYCHIATRICA SCANDINAVICA, Issue 3 2010F. Hardeveld Hardeveld F, Spijker J, De Graaf R, Nolen WA, Beekman ATF. Prevalence and predictors of recurrence of major depressive disorder in the adult population. Objective:, Knowledge of the risk of recurrence after recovery of a major depressive disorder (MDD) is of clinical and scientific importance. The purpose of this paper was to provide a systematic review of the prevalence and predictors of recurrence of MDD. Method:, Studies were searched in Medline en PsychINFO using the search terms ,recur*', ,relaps*', ,depress*', ,predict*' and course. Results:, Recurrence of MDD in specialised mental healthcare settings is high (60% after 5 years, 67% after 10 years and 85% after 15 years) and seems lower in the general population (35% after 15 years). Number of previous episodes and subclinical residual symptoms appear to be the most important predictors. Gender, civil status and socioeconomic status seem not related to the recurrence of MDD. Conclusion:, Clinical factors seem the most important predictors of recurrence. Data from studies performed in the general population and primary care on the recurrent course of MDD are scarce. [source] Efficacy and safety of two treatment algorithms in bipolar depression consisting of a combination of lithium, lamotrigine or placebo and paroxetineACTA PSYCHIATRICA SCANDINAVICA, Issue 3 2010M. L. M. Van Der Loos van der Loos MLM, Mulder P, Hartong EGThM, Blom MBJ, Vergouwen AC, van Noorden MS, Timmermans MA, Vieta E, Nolen WA, for the LamLit Study Group. Efficacy and safety of two treatment algorithms in bipolar depression consisting of a combination of lithium, lamotrigine or placebo and paroxetine. Objective:, In a previous paper, we reported about the efficacy of the addition of lamotrigine to lithium in patients with bipolar depression. In the second phase of this study paroxetine was added to ongoing treatment in non-responders. Method:, Bipolar depressed patients (n = 124) treated with lithium were randomized to addition of lamotrigine or placebo. In non-responders after 8 weeks, paroxetine 20 mg was added for another 8 weeks to ongoing treatment. Results:, After 8 weeks the improvement in patients treated with lamotrigine vs. patients treated with placebo was significant. After addition of paroxetine this difference disappeared as a result of greater further improvement in the non-responders to placebo. Conclusion:, Addition of lamotrigine to lithium was found effective in bipolar depressed patients. Further addition of paroxetine in non-responders to lithium plus lamotrigine did not appear to provide additional benefit, while it appeared to do so in non-responders to lithium plus placebo. [source] An algorithm for the pharmacological treatment of depressionACTA PSYCHIATRICA SCANDINAVICA, Issue 3 2010J. Spijker Spijker J, Nolen WA. An algorithm for the pharmacological treatment of depression. Objective:, Non-response to treatment with antidepressants (AD) is a clinical problem. Method:, The algorithm for pharmacological treatment of the Dutch multidisciplinary guideline for depression is compared with four other algorithms. Results:, The Dutch algorithm consists of five subsequent steps. Treatment is started with one out of many optional ADs (step 1); in case of non-response after 4,10 weeks, best evidence is for switching to another AD (step 2); next step is augmentation with lithium as the best option (step 3); the next step is a monoamine oxidase inhibitor (MAOI) (step 4); and finally electroconvulsive therapy (step 5). There are major differences with other algorithms regarding timing of augmentation step, best agents for augmentation and role of MAOI. Conclusion:, Algorithms for AD treatment vary according to national and local preferences. Although the evidence for most of the treatment strategies is rather meagre, an AD algorithm appears to be an useful instrument in clinical practice. [source] Treatment of unipolar psychotic depression: a randomized, double-blind study comparing imipramine, venlafaxine, and venlafaxine plus quetiapineACTA PSYCHIATRICA SCANDINAVICA, Issue 3 2010J. Wijkstra Wijkstra J, Burger H, van den Broek WW, Birkenhäger TK, Janzing JGE, Boks MPM, Bruijn JA, van der Loos MLM, Breteler LMT, Ramaekers GMGI, Verkes RJ, Nolen WA. Treatment of unipolar psychotic depression: a randomized, double-blind study comparing imipramine, venlafaxine, and venlafaxine plus quetiapine. Objective:, It remains unclear whether unipolar psychotic depression should be treated with an antidepressant and an antipsychotic or with an antidepressant alone. Method:, In a multi-center RCT, 122 patients (18,65 years) with DSM-IV-TR psychotic major depression and HAM-D-17 , 18 were randomized to 7 weeks imipramine (plasma-levels 200,300 ,g/l), venlafaxine (375 mg/day) or venlafaxine,quetiapine (375 mg/day, 600 mg/day). Primary outcome was response on HAM-D-17. Secondary outcomes were response on CGI and remission (HAM-D-17). Results:, Venlafaxine,quetiapine was more effective than venlafaxine with no significant differences between venlafaxine,quetiapine and imipramine, or between imipramine and venlafaxine. Secondary outcomes followed the same pattern. Conclusion:, That unipolar psychotic depression should be treated with a combination of an antidepressant and an antipsychotic and not with an antidepressant alone, can be considered evidence based with regard to venlafaxine,quetiapine vs. venlafaxine monotherapy. Whether this is also the case for imipramine monotherapy is likely, but cannot be concluded from the data. [source] SPECIAL SECTION: EVALUATION OF THE WESTERN AUSTRALIAN CANNABIS INFRINGEMENT NOTICE SCHEME,PHASE 1: Community attitudes towards cannabis law and the proposed Cannabis Infringement Notice scheme in Western AustraliaDRUG AND ALCOHOL REVIEW, Issue 4 2005JAMES FETHERSTON Abstract Western Australia (WA) became the fourth Australian jurisdiction to adopt a prohibition with civil penalties scheme for minor cannabis offences when its Cannabis Infringement Notice (CIN) scheme became law on 22 March 2004. Previous criminological research has demonstrated the importance of public attitudes towards the law in determining the effectiveness of legislation. This survey represents the first phase of a pre-post study that attempted to gauge public attitudes towards the legal status of cannabis, the proposed legislative reforms surrounding the drug and their likely effects. A random telephone survey of 809 members of the WA population was conducted prior to the implementation of the new laws with a view to exploring contemporary views of the existing legal status of cannabis, attitudes to the proposed legislative model and respondent perceptions of its likely effects. Despite cannabis being viewed negatively by large numbers of the sample, criminal penalties for minor cannabis offences were viewed as inappropriate and ineffective. Once explained, the proposed civil penalty scheme was viewed as ,a good idea' by 79% of the sample, despite significant differences due to personal experience of cannabis use, political affiliation, religiosity and age of offspring. Most believed that the legislative change would not result in changes to levels of cannabis use (70%) or ease of obtaining cannabis (59%). These data suggest that prior to its implementation the new legislation was highly acceptable to the majority of the community. These baseline data will be compared with data to be collected at the post-change phase of the study to allow empirical observations of attitudinal and behavioural changes occurring in the community. [source] Expected impacts of the Cannabis Infringement Notice scheme in Western Australia on regular users and their involvement in the cannabis marketDRUG AND ALCOHOL REVIEW, Issue 4 2005FRANCOISE CHANTELOUP Abstract The effect on the cannabis market is one area of interest in the evaluation of the new ,prohibition with civil penalties' scheme for minor cannabis offences in WA. One goal of the scheme is to reduce the proportion of cannabis consumed that is supplied by large-scale suppliers that may also supply other drugs. As part of the pre-change phase of the evaluation, 100 regular (at least weekly) cannabis users were given a qualitative and quantitative interview covering knowledge and attitudes towards cannabis law, personal cannabis use, market factors, experience with the justice system and impact of legislative change. Some 85% of those who commented identified the changes as having little impact on their cannabis use. Some 89% of the 70 who intended to cultivate cannabis once the CIN scheme was introduced suggested they would grow cannabis within the two non-hydroponic plant-limit eligible for an infringement notice under the new law. Only 15% believed an increase in self-supply would undermine the large scale suppliers of cannabis in the market and allow some cannabis users to distance themselves from its unsavoury aspects. Only 11% said they would enter, or re-enter, the cannabis market as sellers as a result of the scheme introduction. Most respondents who commented believed that the impact of the legislative changes on the cannabis market would be negligible. The extent to which this happens will be addressed in the post-change phase of this research. Part of the challenge in assessing the impact of the CIN scheme on the cannabis market is that it is distinctly heterogeneous. [source] Potential impacts on the incidence of fatal heroin-related overdose in Western Australia: a time-series analysisDRUG AND ALCOHOL REVIEW, Issue 4 2002KIM HARGREAVES Abstract In response to the rising concerns about the rate of heroin-related fatalities, overdose prevention campaigns, run by both users' organizations and government agencies, have been implemented in a number of states across Australia. In Western Australia (WA) in mid-1997, various overdose prevention initiatives were implemented. These included the implementation of a protocol limiting police presence at overdose events; the commencement of naloxone administration by ambulance staff; and the establishment of the Opiate Overdose Prevention Strategy (OOPS) which provided follow-up for individuals treated for overdose in emergency departments. This paper reports the results of a multiple linear regression analysis of 60 months of time-series data, both prior to and following the implementation of these interventions, to determine their impact on the number of fatal heroin overdoses in WA. The model employed in the analysis controlled for changes over time in proxy indicators of use and community concerns about heroin, as well as market indicators. The results suggest that, although the interventions implemented have managed to reduce the expected number of fatalities, they have become less successful in doing so as time passes. This has implications for both existing and potential interventions to reduce fatal heroin-related overdose. [source] A tale of CIN,the Cannabis Infringement Notice scheme in Western AustraliaADDICTION, Issue 5 2010Simon Lenton ABSTRACT Aims To describe the development and enactment of the Western Australian (WA) Cannabis Infringement Notice scheme and reflect on the lessons for researchers and policy-makers interested in the translation of policy research to policy practice. Methods An insiders' description of the background research, knowledge transfer strategies and political and legislative processes leading to the enactment and implementation of the WA Cannabis Control Act 2003. Lenton and Allsop were involved centrally in the process as policy-researcher and policy-bureaucrat. Results In March 2004, Western Australia became the fourth Australian jurisdiction to adopt a ,prohibition with civil penalties' scheme for possession and cultivation of small amounts of cannabis. We reflect upon: the role of research evidence in the policy process; windows for policy change; disseminating findings when apparently no one is listening; the risks and benefits of the researcher as advocate; the differences between working on the inside and outside of government; and the importance of relationships, trust and track record. Conclusions There was a window of opportunity and change was influenced by research that was communicated by a reliable and trusted source. Those who want to conduct research that informs policy need to understand the policy process more clearly, look for and help create emerging windows that occur in the problem and political spheres, and make partnerships with key stakeholders in the policy arena. The flipside of the process is that, when governments change, policy born in windows of opportunity can be a casualty. [source] Assortative mating between adjacent populations of threespine stickleback (Gasterosteus aculeatus)ECOLOGY OF FRESHWATER FISH, Issue 1 2004R. J. Scott Abstract,,, The idea that sexual selection can lead to rapid evolution of premating isolation among independent populations (speciation) has been controversial, but is rapidly gaining acceptance among many evolutionary biologists as empirical examples accumulate. A survey of male signals and female preferences (mate recognition systems, MRSs) across the contact zone between divergent populations of threespine stickleback (Gasterosteus aculeatus) in Conner Creek, WA, revealed a spatial pattern of MRSs that is consistent with speciation via sexual selection. Females from locations that possessed melanic males preferred melanic males whereas females from populations possessing typical mosaic males (red chin, blue iris, and blue-green dorsum) preferred mosaic males. I argue that sensory drive sexual selection, acting through geographically varying spectral properties, is responsible for the observed population differences and premating isolation between the adjacent populations. [source] Detection of glycolate oxidase gene glcD diversity among cultured and environmental marine bacteriaENVIRONMENTAL MICROBIOLOGY, Issue 10 2006W. W. Y. Lau Summary Of eight laboratory cultures of marine ,- and ,- Proteobacteria tested, growth on glycolate as a sole carbon source was detected for only three species: Pseudomonas stutzeri, Oceanimonas doudoroffii and Roseobacter sp. isolate Y3F. Degenerate polymerase chain reaction (PCR) primers were designed to amplify glcD, which encodes the D-subunit of the enzyme glycolate oxidase; glcD could be amplified only from those cultures that grew on glycolate. The PCR primers were used to explore glcD diversity in four field samples collected from different ocean environments: an Atlantic Gulf Stream Ring, sampled above and below the thermocline and two Pacific coastal sites, Parks Bay and San Juan Channel, WA. Environmental glcD sequences belonged to six major bacterial phylogenetic groups, with most sequences forming novel clades with no close relatives. Different patterns of glcD diversity were observed within and between the two nutrient regimes. Comparison of glcD and 16S rDNA diversity and analyses of available bacterial genomes and a metgenomic library from the Sargasso Sea show that glycolate-utilizing potential exists in only a subset of bacteria. Glycolate is produced in marine environments mainly by phytoplankton. Examination of glcD diversity will aid in understanding the influence of phytoplankton on bacterial community structure. [source] Harbor seals (Phoca vitulina) in British Columbia, Canada, and Washington State, USA, reveal a combination of local and global polychlorinated biphenyl, dioxin, and furan signalsENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 1 2004Peter S. Ross Abstract The harbor seal (Phoca vitulina) can serve as a useful indicator of food web contamination by persistent organic pollutants (POPs) because of its high trophic level, wide distribution in temperate coastal waters of the Northern Hemisphere, and relative ease of capture. In 1996 through 1997, we live-captured 60 harbor seal pups from three regions, spanning remote (Queen Charlotte Strait, BC, Canada), moderately industrialized (Strait of Georgia, BC, Canada), and heavily industrialized (Puget Sound, WA, USA) marine basins straddling the Canada-United States border. Biopsy samples of blubber were taken and analyzed for congener-specific polychlorinated biphenyls (PCBs), polychlorinated dibenzo- p -dioxins (PCDDs), and polychlorinated dibenzofurans (PCDFs) by using high-resolution gas chromatography-high-resolution mass spectrometry. Harbor seals in Puget Sound were heavily contaminated with PCBs, whereas seals from the Strait of Georgia had relatively high concentrations of PCDDs and PCDFs. Pattern evaluation and principal components analysis suggested that proximity to sources influenced the mixture to which seals were exposed, with those inhabiting more remote areas being exposed to lighter PCB congeners (those with lower Henry's law constant and KOW) that disperse more readily through atmospheric and other processes. Total toxic equivalents to 2,3,7,8-tetrachlorodibenzo- p -dioxin for the PCBs, PCDDs, and PCDFs suggest that Puget Sound seals are at greatest risk for adverse health effects, and that PCBs represent the class of dioxinlike contaminants of greatest concern at all sites. [source] A field validation of two sediment-amphipod toxicity testsENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 7 2002Steven P. Perraro Abstract A field validation study of two sediment-amphipod toxicity tests was conducted using sediment samples collected subtidally in the vicinity of a polycyclic aromatic hydrocarbon (PAH)-contaminated Superfund site in Elliott Bay (WA, USA). Sediment samples were collected at 30 stations with a 0.1 m2 grab from which subsamples were taken for sediment toxicity testing and geochemical and macrofaunal analyses. Standard 10-d sediment-amphipod toxicity tests were conducted with Rhepoxynius abronius and Leptocheirus plumulosus. Sediments were analyzed for 33 PAHs, pentachlorophenol, polychlorinated biphenyls, acid-volatile sulfide, simultaneously extracted metals (Cd, Cu, Zn, Pb, Ni), total organic carbon, and grain size. Sediment temperature, oxygen-reduction potential, water depth, and interstitial water salinity were also measured. Polycyclic aromatic hydrocarbons, quantified as total PAH toxic units (TUPAH), were confirmed to be an important common causal agent of the changes in the two toxicity test (% survival R. abronius, % survival L. plumulosus) and five macrofaunal community (number of species, S; numerical abundance, A; total biomass, B; Swartz's dominance index, SDI; Brillouin's index, H) endpoints. Two other macrofaunal community metrics (the complement of Simpson's index, 1 , SI, and McIntosh's index, MI) were less sensitive to TUPAH than the two toxicity test endpoints. The sensitivities of R. abronius and L. plumulosus to TUPAH were statistically indistinguishable. Field validations were conducted by testing the association between or among each toxicity test endpoint, each of seven macrofaunal community metrics (S, A, B, SDI, H, 1 , SI, MI), and TUPAH by (1) Spearman's coefficient of rank correlation, (2) Kendall's coefficient of concordance, (3) G tests of independence, and (4) regression analysis. Some field validations based on multivariable tests of association (e.g., points 2 and 3) among toxicity test, field, and stressor endpoints produced false positive results. Both toxicity test endpoints were validated as indicators of changes in S, A, SDI, and H by all the methods tested. The resolution power of the relationships between the laboratory toxicity test and macrofaunal field endpoints was low (, three classes) but sufficient to discriminate ecologically important effects. We conclude that standard sediment-amphipod toxicity tests are ecologically relevant and that, under the proper conditions, their results can be used for lab-to-field extrapolation. [source] Disability payments, drug use and representative payees: an analysis of the relationshipsADDICTION, Issue 7 2003James A. Swartz ABSTRACT Aims This study attempted to determine: if US federal cash disability payments increase the use of cocaine or opiates among those requalifying for supplemental security income (SSI) disability benefits compared with those who lost benefits; if drug use peaks at the beginning of the month after the receipt of the disability cash disbursement; and if money management by representative payees of requalifying SSI recipients suppresses drug use. Design A multi-site, prospective, 2 year longitudinal design was used with follow-up interviews conducted every 6 months. Urine samples were collected at the final three follow-up interviews. Setting Data were collected in Chicago, IL, Los Angeles, CA, and Seattle, WA, USA. Participants This study used a randomly selected sample of 740 former recipients of SSI who had received disability benefits for drug addiction and alcoholism (DA&A) in 1996, were between the ages of 21 and 59 years, had not received concurrent social security disability insurance and provided testable urine samples and complete self-report data for at least one follow-up interview. Measurements Independent variables included demographics, SSI status at follow-up, representative payee status, drug treatment participation and income. Time of drug testing was operationalized as the first 10 days of the month versus the last 20,21 days based on when the urine sample was collected. The dependent variables were cocaine and opiate use, determined by urinalysis results. Findings Participants were 28% more likely to test positive for cocaine use in the first 10 days of the month than later in the month. This effect was general across all subjects and was not restricted to those receiving SSI benefits. No such effect was found for opiate use. Receiving SSI benefits did not increase cocaine or opiate use generally, nor did having a representative payee suppress use. Conclusions The findings do not support the contentions that federal cash benefits appreciably increase drug use or that representative payees discourage use, at least when use is defined dichotomously. The ,check effect' for cocaine use appears to be general and not confined to those receiving federal cash benefits. The lack of a ,check effect' for opiate use is probably the result of the difference between a relatively steady state of opiate use associated with addiction and a binge pattern of cocaine use triggered by suddenly flush resources. [source] Blood morphine levels in naltrexone-exposed compared to non-naltrexone-exposed fatal heroin overdosesADDICTION BIOLOGY, Issue 3 2003DIANE ARNOLD-REED The aim of this study was to investigate the association between prior exposure to naltrexone and increased risk of fatal heroin overdose using a review of toxicology reports for heroin-related fatalities between July 1997 to August 1999 for two groups: those treated with oral naltrexone and those who were not treated. Additional information for the oral naltrexone group was obtained from clinic files. Naltrexone-treated deaths were identified from the patient database at the Australian Medical Procedures Research Foundation (AMPRF), Perth, Western Australia (WA) through the Western Australian Department of Health, Data Linkage Project. Non-treated cases were identified from the database at the Forensic Science Laboratory, State Chemistry Centre (WA). We identified and investigated blood morphine concentrations following 21 fatal heroin overdoses with prior exposure to naltrexone and in 71 non-naltrexone-exposed cases over the same time period. The proportion of deaths where heroin use was a major contributing factor was little different in the non-naltrexone compared to the naltrexone-exposed group. Furthermore, in ,acute opiate toxicity' deaths, blood morphine levels were lower in non-naltrexone-exposed compared with naltrexone-exposed cases. Although there was a higher number of deaths designated as rapid (i.e. occurring within 20 minutes) in the naltrexone-exposed (89%) compared with the non-exposed group (72%) this was not statistically significant. Other drug use in relation to heroin-related fatalities is discussed. Findings do not support the hypothesis that prior exposure to naltrexone increases sensitivity to heroin toxicity. [source] The effect of fixed-count subsampling on macroinvertebrate biomonitoring in small streamsFRESHWATER BIOLOGY, Issue 2 2000Craig P. Doberstein Summary 1When rigorous standards of collecting and analysing data are maintained, biological monitoring adds valuable information to water resource assessments. Decisions, from study design and field methods to laboratory procedures and data analysis, affect assessment quality. Subsampling - a laboratory procedure in which researchers count and identify a random subset of field samples - is widespread yet controversial. What are the consequences of subsampling? 2To explore this question, random subsamples were computer generated for subsample sizes ranging from 100 to 1000 individuals as compared with the results of counting whole samples. The study was done on benthic invertebrate samples collected from five Puget Sound lowland streams near Seattle, WA, USA. For each replicate subsample, values for 10 biological attributes (e.g. total number of taxa) and for the 10-metric benthic index of biological integrity (B-IBI) were computed. 3Variance of each metric and B-IBI for each subsample size was compared with variance associated with fully counted samples generated using the bootstrap algorithm. From the measures of variance, we computed the maximum number of distinguishable classes of stream condition as a function of sample size for each metric and for B-IBI. 4Subsampling significantly decreased the maximum number of distinguishable stream classes for B-IBI, from 8.2 for fully counted samples to 2.8 classes for 100-organism subsamples. For subsamples containing 100,300 individuals, discriminatory power was low enough to mislead water resource decision makers. [source] The Historical Geographies of Showing Livestock: a Case Study of the Perth Royal Show, Western AustraliaGEOGRAPHICAL RESEARCH, Issue 3 2010RICHARD YARWOOD Abstract This paper examines changes in the entries of livestock to competitions at the Perth Royal Show in the course of the twentieth century. It identifies trends in the showing of animals at the Show and explains these with reference to the wider geographies of state and national agricultural change in Western Australia (WA). In doing so, it provides a longitudinal perspective on the socioeconomic contexts of farming in WA and identifies some of the key cultural and economic drivers that have influenced livestock farming in that locality. In turn, these findings contribute to wider understandings of the global countryside and of the imagined ruralities that exist within and beyond the spaces of showgrounds. [source] Broad-scale environmental response and niche conservatism in lacustrine diatom communitiesGLOBAL ECOLOGY, Issue 5 2010Joseph R. Bennett ABSTRACT Aim, (1) To resolve theoretical debates regarding the role of environment versus dispersal limitation, the conservatism of niches across distances and the prevalence of environmental specialists in diatom communities. (2) To provide guidance on the use of diatom communities and other microbial analogues to analyse ecological response to environmental change. Location, Eight hundred and ninety-one lakes in five regional datasets from north-western Europe and four regional datasets from north-eastern North America. Methods, Lacustrine diatom communities were analysed at three scales: inter-continental, intra-continental and regional. Nested partial redundancy analyses (RDAs) were used to determine spatial versus environmental components of community variation. Weighted-averaging (WA) regression and calibration, as well as logistic and quadratic regressions, were used to detect niche conservatism and the prevalence of environmental specialists. Results, Community patterns indicate that dispersal limitation acts predominantly at the inter-continental scale, while at the regional (less than c. 1,000,000 km2) scale, a single environmental variable (pH) explains more than five times the community variation as spatial (dispersal-related) variables. In addition, pH niche components appear to be conserved at the inter-continental scale, and environmental specialization does not impose relative rarity, as specialists apparently readily disperse to suitable environments. Main conclusions, Analysis at multiple scales is clearly important in determining the influences of community variation. For diatom communities, dispersal limitation acts most strongly at the broadest scales, giving way to environment at the scales considered by most analyses. The availability of a wide variety of propagules with consistent niches across regions indicates that diatom communities reflect the succession of taxa according to local environmental conditions, rather than disequilibrium with the environment or adaptation of local populations. While multi-scale analyses must be undertaken for other groups to resolve debates over community drivers and determine appropriate scales for prediction, for diatoms (and probably other microbial communities), responses to environmental change can be inferred using analogue datasets from large geographic areas. [source] The Impact of Welfare Reform on Insurance Coverage before Pregnancy and the Timing of Prenatal Care InitiationHEALTH SERVICES RESEARCH, Issue 4 2007Norma I. Gavin Objective. This study investigates the impact of welfare reform on insurance coverage before pregnancy and on first-trimester initiation of prenatal care (PNC) among pregnant women eligible for Medicaid under welfare-related eligibility criteria. Data Sources. We used pooled data from the Pregnancy Risk Assessment Monitoring System for eight states (AL, FL, ME, NY, OK, SC, WA, and WV) from 1996 through 1999. Study Design. We estimated a two-part logistic model of insurance coverage before pregnancy and first-trimester PNC initiation. The impact of welfare reform on insurance coverage before pregnancy was measured by marginal effects computed from coefficients of an interaction term for the postreform period and welfare-related eligibility and on PNC initiation by the same interaction term and the coefficients of insurance coverage adjusted for potential simultaneous equation bias. We compared the estimates from this model with results from simple logistic, ordinary least squares, and two-stage least squares models. Principal Findings. Welfare reform had a significant negative impact on Medicaid coverage before pregnancy among welfare-related Medicaid eligibles. This drop resulted in a small decline in their first-trimester PNC initiation. Enrollment in Medicaid before pregnancy was independent of the decision to initiate PNC, and estimates of the effect of a reduction in Medicaid coverage before pregnancy on PNC initiation were consistent over the single- and two-stage models. Effects of private coverage were mixed. Welfare reform had no impact on first-trimester PNC beyond that from reduced Medicaid coverage in the pooled regression but separate state-specific regressions suggest additional effects from time and income constraints induced by welfare reform may have occurred in some states. Conclusions. Welfare reform had significant adverse effects on insurance coverage and first-trimester PNC initiation among our nation's poorest women of childbearing age. Improved outreach and insurance options for these women are needed to meet national health goals. [source] Alcohol consumption and risk of prostate cancer in middle-aged menINTERNATIONAL JOURNAL OF CANCER, Issue 1 2005W. Marieke Schoonen Abstract Alcohol consumption is a modifiable lifestyle factor that may affect prostate cancer risk. Alcohol alters the hormonal milieu and contains chemical substances such as flavonoids (red wine), which may alter tumor cell growth. Data from a population-based case-control study in King County, WA, were utilized to evaluate the association of alcohol consumption with prostate cancer in middle-aged men. A total of 753 newly diagnosed prostate cancer cases, 40,64 years of age, participated in the study. Seven hundred three control subjects, frequency matched to cases by age, were selected through random digit dialing. All participants completed an in-person interview on lifetime alcohol consumption and other risk factors for prostate cancer. Logistic regression models were used to estimate odds ratios (OR) and assess significance (95% confidence intervals [CI]). All tests of statistical significance were two-sided. No clear association with prostate cancer risk was seen for overall alcohol consumption. Each additional glass of red wine consumed per week showed a statistically significant 6% decrease in relative risk (OR = 0.94; 95% CI = 0.90,0.98), and there was evidence for a decline in risk estimates across increasing categories of red wine intake (trend p = 0.02). No clear associations were seen for consumption of beer or liquor. Our present study suggests that consumption of beer or liquor is not associated with prostate cancer. There may be, however, a reduced relative risk associated with increasing level of red wine consumption. Further research is needed to evaluate the potential negative association between red wine intake and prostate cancer risk. [source] ISLAMIC DA ,WA AND CHRISTIAN MISSION: TOWARDS A COMPARATIVE ANALYSISINTERNATIONAL REVIEW OF MISSION, Issue 353 2000David A. Kerr First page of article [source] Breeding biology of ostriches (Struthio camelus) in the Serengeti ecosystem, TanzaniaAFRICAN JOURNAL OF ECOLOGY, Issue 3 2009Flora J. Magige Abstract Ostrich breeding behaviour in the Serengeti ecosystem, Tanzania was investigated for differences in laying dates between low altitude western area (WA) and high altitude eastern area (EA) populations. Ostriches in WA laid eggs significantly earlier than in EA. The differences could be attributed to topography and rainfall pattern. Reliable rains in lower altitudes ensure availability of food that in turn influences the whole process of the reproductive cycle. Clutches were contributed by several females with a nest having up to 38 eggs. We also compared the frequency of observation of predators, ostriches, nests, ,singletons' (single eggs laid randomly) and broods between the two areas. There was no significant difference between WA and EA in 1) ostrich/nest ratio, indicating similar breeding densities; 2) ostrich/predator and predator/nest ratios, indicating that predation pressure was equally high; 3) nest/singleton and predator/singleton ratios, indicating that loss of nests did not vary between areas. However, there were significantly more predators, nests and ostriches compared to broods in EA than in WA, indicating a significantly lower reproductive success in EA. Using metapopulation terminology, ostriches in EA could be regarded as a ,sink' population and those in WA as a ,source' population, but investigations over longer time-periods are needed to further resolve if this is the case. Résumé Le comportement reproducteur des autruches dans l'écosystème du Serengeti, en Tanzanie, a étéétudié pour voir les différences dans les dates de pontes entre les populations de la zone occidentale (WA) à basse altitude et de la zone orientale (EA) à plus haute altitude. Les autruches de la WA pondent significativement plus tôt que celles de l'EA. Les différences peuvent être attribuées à la topographie et au régime de la pluviosité. Les pluies fiables à plus basse altitude garantissent la disponibilité de la nourriture, ce qui influence dès lors tout le processus du cycle reproducteur. Des couvées réunissaient les ,ufs de plusieurs femelles , un nid a eu jusqu'à 38 ,ufs. Nous avons aussi comparé la fréquence d'observations de prédateurs, d'autruches, de nids, de «ingletons» (des ,ufs solitaires pondus au hasard) et de nichées entre les deux zones. Il n'y a pas de différence significative entre WA et EA pour 1) le ratio autruches/nids, ce qui indique des densités de reproduction similaires; 2) le ratio autruches/prédateurs et le ratio prédateurs/nids, ce qui indique que la pression de la prédation est aussi haute des deux côtés; et 3) le ratio nids/singletons et le ratio prédateurs/singletons, ce qui indique que la perte des nids ne variait pas entre les zones. Cependant, il y avait significativement plus de prédateurs, de nids et d'autruches par rapport aux nichées dans l'EA que dans la WA, ce qui indique une réussite de la reproduction significativement plus faible dans la EA. En utilisant la terminologie de la métapopulation, les autruches de la EA peuvent être considérées comme une population «puits» et celles de la WA comme une population «source», mais il faudrait faire des recherches de plus longue durée pour montrer si c'est bien le cas. [source] CD99 Immunoreactivity in Metastatic Malignant MelanomaJOURNAL OF CUTANEOUS PATHOLOGY, Issue 1 2005AE Wilkerson CD99, also known as p30/32, is a glycoprotein product of the MIC2 gene, which is located on the short arm of both chromosome X and Y. This transmembrane protein was originally utilized in immunohistochemistry as a unique marker for Ewing sarcoma, other primitive neuroectodermal tumors, and more recently in a wide variety of tumors. It's expression in malignant melanoma (MM) has not been well documented. A recent study at our institution demonstrated membranous staining in approximately 61% of primary MM. As CD99 is expressed by hematopoeitic cells, it has been proposed as a mechanism for lymphocytes to gain access to the vasculature.1 This study is designed to determine if CD99 expression in melanoma cells has a similar role using cases of metastatic MM from our archives. Our evaluation shows that 13 of 28 cases (46.4%) demonstrated membranous CD99 staining. A case of this magnitude has not been previously reported. Reference: 1. Shenkel AR, Mamdouh Z, Chen X, Liebman RM, Muller WA. CD99 plays a major role in the migration of monocytes through endothelial junctions. Nature Immunol 2002;3:143,150. [source] Properties of Poly(lactide)-Coated Paperboard for the Use of 1-Way Paper CupJOURNAL OF FOOD SCIENCE, Issue 2 2009J.-W. Rhim ABSTRACT:, Poly(lactide)-coated paperboards were prepared by a solution coating method, and the effect of coating to improve properties of paperboard used for the manufacturing of 1-way paper cups was tested. Surface of PLA-coated paperboards was smooth and shiny like PE-coated paperboard, and the coating weight and thickness increased linearly with increasing PLA concentration of coating solution. Tensile strength (TS) and elongation at break (E) of the paperboard also increased after PLA coating. Water vapor barrier or water-resistant properties tested, such as water vapor permeability (WVP), water absorptiveness (WA), and contact angle (CA) of water drop, indicated that water resistance of the paperboard was improved through surface coating with PLA. The increase in water resistance of PLA-coated paperboards was mainly due to the hydrophobicity of PLA and the improvement of water barrier properties increased depending on the PLA concentration. In addition, PLA-coated paperboard showed strong heat sealing property when coated with more than 1 w/v% of PLA. Wet strength of PLA-coated (3, w/v%) paperboard was comparable to or greater than that of PE-coated paperboard. All the test results indicated that the PLA-coated paperboard can be exploited for the manufacturing of 1-way paper cups as an alternative to the PE-coated paperboard. [source] Improving food purchasing choices through increased understanding of food labels, using itemized till receipts to measure these changesJOURNAL OF HUMAN NUTRITION & DIETETICS, Issue 4 2008P.M. Rigby Background:, Rising obesity and the associated risks of diabetes and heart disease require changes in diet to bring about healthier eating. To achieve this, people need to understand nutrition and daily requirements but are frequently confused by nutrition information on food labels. The introduction of the Food Standards Agency's ,Traffic Light' and the alternative ,Guideline Daily Amount' systems may help or further confuse the public. A previous study showed that although 63% of study participants read labels, only 25% claimed to understand them, also knowledge of nutrient requirements and functions was low (Rigby, 2004). Ransley et al., (2001) have shown that till receipts can be used to estimate fat and energy intake. The aim of the current study was to investigate whether an intervention designed to improve understanding of nutrition and labels could improve food purchases and whether these changes could be measured from till receipts. Method:, Participants were recruited from the general public (80 female; 23 males) (age <30 years (23), 31,45 years (25), 46,60 years (30), >60 years (22). Subjects were randomly assigned to either the intervention group (n = 78) who were provided with an information booklet and credit card sized nutrition and labelling information to use when shopping, or the control group (n = 25), who received the information after four weeks of normal shopping. The intervention group provided an initial till receipt pre intervention and was then given the nutrition and labelling material. Further till receipts were returned from successive shopping trips over the following 4 weeks. The nine categories of food used for comparison were: fruit and vegetables, saturated fats, monounsaturated fats, polyunsaturated fats, white cereals, wholegrain cereals, processed foods, full fat and reduced fat items. Ethics approval was obtained from NWW Wales NHS Trust ethics committee. Results:, Each of the nine food categories on till receipts were calculated as a percentage of the total shopping, excluding non-food items. General linear model repeated measures analyses showed differences between study participants' food purchases. For the intervention group, purchases in three of the nine food categories showed significant improvements: increased purchases of fruits and vegetables (P < 0.001); reduction in purchases of saturated fats (P < 0.001); and reduction of white cereal purchases (P < 0.050). The control group showed no differences in any category. Discussion:, Although the intervention group did show improvements in most of the other food categories, they were not statistically significant. Positive changes were found in seven of the nine categories, with only one, wholegrain cereals, showing a decrease in purchases rather than an increase. The control group displayed a random pattern over the four till receipts, with eight categories either showing negative change or no change; only one showed a positive change. The disproportionate group sizes may mean that it is not be possible to draw firm conclusions regarding the effectiveness of the intervention. Conclusions:, This study demonstrates that positive changes in improving food-purchasing choices, as measured by till receipts, can be made by using educational interventions. Further larger studies using routinely collected supermarket data would enable the study to be replicated on a much larger scale. References, Ransley, J.K., Donnelly, J.K., Khara, T.N., Botham, H., Arnott, H., Greenwood, D.C. & Cade, J.E. (1991) The use of supermarket till receipts to determine the fat and energy intake in a UK population. Public Health Nutr. 4, 1279,1286. Rigby, P. (2004) Effecting change. Understanding nutritional information. Can increased knowledge and understanding in relation to nutritional information bring about a change in eating habits? PhD Thesis WA: Bangor University. [source] Structural and Expressional Variations of the Mitochondrial Genome Conferring the Wild Abortive Type of Cytoplasmic Male Sterility in RiceJOURNAL OF INTEGRATIVE PLANT BIOLOGY, Issue 6 2007Zhen-Lan Liu Abstract The so-called "wild abortive" (WA) type of cytoplasmic male sterility (CMS) derived from a wild rice species Oryza rufipogon has been extensively used for hybrid rice breeding. However, extensive analysis of the structure of the related mitochondrial genome has not been reported, and the CMS-associated gene(s) remain unknown. In this study, we exploited a mitochondrial genome-wide strategy to examine the structural and expressional variations in the mitochondrial genome conferring the CMS. The entire mitochondrial genomes of a CMS-WA line and two normal fertile rice lines were amplified by Long-polymerase chain reaction into tilling fragments of up to 15.2 kb. Restriction and DNA blotting analyses of these fragments revealed that structural variations occurred in several regions in the WA mitochondrial genome, as compared to those of the fertile lines. All of the amplified fragments covering the entire mitochondrial genome were used as RNA blot probes to examine the mitochondrial expression profile among the CMS-WA and fertile lines. As a result, only two mRNAs were found to be differentially expressed between the CMS-WA and the fertile lines, which were detected by a probe containing the nad5 and orf153 genes and the other having the ribosomal protein gene rpl5, respectively. These mRNAs are proposed to be the candidates for further identification and functional studies of the CMS gene. [source] Multicontrast black-blood MRI of carotid arteries: Comparison between 1.5 and 3 tesla magnetic field strengthsJOURNAL OF MAGNETIC RESONANCE IMAGING, Issue 5 2006Vasily L. Yarnykh PhD Abstract Purpose To compare black-blood multicontrast carotid imaging at 3T and 1.5T and assess compatibility between morphological measurements of carotid arteries at 1.5T and 3T. Materials and Methods Five healthy subjects and two atherosclerosis patients were scanned in 1.5T and 3T scanners with a similar protocol providing transverse T1 -, T2 -, and proton density (PD)-weighted black-blood images using a fast spin-echo sequence with single- (T1 -weighted) or multislice (PD-/T2 -weighted) double inversion recovery (DIR) preparation. Wall and lumen signal-to-noise ratio (SNR) and wall/lumen contrast-to-noise ratio (CNR) were compared in 44 artery cross-sections by paired t -test. Interscanner variability of the lumen area (LA), wall area (WA), and mean wall thickness (MWT) was assessed using Bland-Altman analysis. Results Wall SNR and lumen/wall CNR significantly increased (P < 0.0001) at 3T with a 1.5-fold gain for T1 -weighted images and a 1.7/1.8-fold gain for PD-/T2 -weighted images. Lumen SNR did not differ for single-slice DIR T1 -weighted images (P = 0.2), but was larger at 3T for multislice DIR PD-/T2 -weighted images (P = 0.01/0.03). The LA, WA, and MWT demonstrated good agreement with no significant bias (P 0.5), a coefficient of variation (CV) of <10%, and intraclass correlation coefficient (ICC) of >0.95. Conclusion This study demonstrated significant improvement in SNR, CNR, and image quality for high- resolution black-blood imaging of carotid arteries at 3T. Morphologic measurements are compatible between 1.5T and 3T. J. Magn. Reson. Imaging 2006. © 2006 Wiley-Liss, Inc. [source] Ascertainment of birth defects: The effect on completeness of adding a new source of dataJOURNAL OF PAEDIATRICS AND CHILD HEALTH, Issue 6 2000C Bower Background: The Western Australian (WA) Birth Defects Registry aims for complete ascertainment of birth defects in WA, but the proportions of birth defects in rural areas and in Aboriginal children are lower than in metropolitan and non-Aboriginal children. The effect on ascertainment of adding data from the Rural Paediatric Service (RPS) was investigated. Method: A file of all cases of birth defects for children born 1980,1997 and recorded on the RPS database was linked to the Registry. Results: The addition of this new data source had little effect on the overall prevalence of birth defects (an increase from 5.38 to 5.41%). There was a slightly greater effect on the prevalence of birth defects in rural residents (4.67%,4.76%) and Aboriginal children (4.55,4.78%), although the prevalence for each of these groups is still less than for metropolitan residents and non-Aboriginal infants, respectively. All major categories of birth defects were represented in the new cases and, in general, their addition made little difference to the prevalence of each category. The exception was fetal alcohol syndrome, which increased from 0.13 per 1000 to 0.18 per 1000 once the 21 new cases from the RPS were added. Conclusion: Complete ascertainment of birth defects is important in developing and evaluating preventive programs, and in investigating clusters of birth defects. [source] Gastroschisis: Early enteral feeds may improve outcomeJOURNAL OF PAEDIATRICS AND CHILD HEALTH, Issue 5 2000M Sharp Objective: Population-based retrospective review of gastroschisis from 1986 to 1996. Methods: This was a retrospective review of gastroschisis. Seventy cases were identified from the Birth Defects Registry of Western Australia (WA). Hospital medical records of live-born cases were reviewed. Results: The live-born incidence of gastroschisis in WA was 2.1 per 10 000 live births for the period 1986,96. The incidence in mothers aged less than 20 years was 8.3-fold that of women aged over 30 years (P < 0.0001). The incidence rate for the period 1995,96 was over twice the rate for 1986,88. Age at first enteral feed was significantly related with length of hospital stay and duration of total parental nutrition (TPN). Each day delay in commencing enteral feed was associated with an increase in hospital stay of 1.05 days and an increase in TPN duration of 1.06 days. The method of delivery of the infant, age at repair, length of anaesthetic time, duration of postoperative paralysis and gestational age was not associated with length of stay or TPN duration. The data were divided into two cohorts: (i) 1986,90; and (ii) 1991,96. There was a statistically significant reduction in hospital stay from a geometric mean of 45.7 (1986,90) to 22.9 days (1991,96). Conclusions: Gastroschisis has a favourable outlook, with 89.7% survival of live births. Over the 10 year period studied, there has been a reduction in length of hospital stay and duration of TPN. The age at which the infant is first fed enteral feeds appears to be important in affecting the length of hospital stay and the duration of TPN, with delays associated with a longer hospital stay and longer TPN duration. [source] Intellectual disability in Western AustraliaJOURNAL OF PAEDIATRICS AND CHILD HEALTH, Issue 3 2000C Bower Objective: To investigate the prevalence of intellectual disability in Western Australia (WA), its causes, prevention, and trends over time. Methodology: Data from an administrative database of intellectual disability in WA were used to report on the trends in intellectual disability in childhood. Results: The prevalence of intellectual disability was 8.3 per 1000 live births in 1980,90. For half the cases, there was no known cause for the intellectual disability. Down syndrome accounted for 14 to 15% of all cases. Since the introduction of newborn screening, no WA-born child participating in the screening program has been diagnosed with intellectual disability as a result of either phenylketonuria or congenital hypothyroidism. The rate of autism spectrum disorders rose from three to six per 10 000 in the 1980,83 WA birth cohort to 10,13 per 10 000 for the 1989,92 cohort. Conclusions: Recent linkage of this administrative database to the WA Maternal and Child Health Research Data Base provides a unique opportunity for more detailed investigation of intellectual disability and its risk factors in a large, well-ascertained population of children. [source] | ||||||||||||||||||||||||||||||||||||||||||||||