Home About us Contact
|
Home About us Contact | ||
|
|
||
Various Responses (various + response)
Selected AbstractsEnhanced Osteoclastogenesis in 4-1BB,Deficient Mice Caused by Reduced Interleukin-10,JOURNAL OF BONE AND MINERAL RESEARCH, Issue 12 2006Hyun-Hee Shin PhD Abstract Enhanced osteoclastogenesis was observed in bone marrow,derived macrophage cells from 4-1BB,deficient mice than in those from wildtype mice. 4-1BB and 4-1BB ligand interaction may play a role at a certain stage of osteoclast formation through increased level of IL-10, a negative regulator of osteoclastogenesis. Introduction: 4-1BB is an inducible T-cell costimulatory molecule and a member of the TNF receptor family. The expression pattern of 4-1BB and 4-1BB ligand (4-1BBL) has suggested that 4-1BB plays a role not only in various responses related to innate immunity but also in bone metabolism. Materials and Methods: Osteoclast formation was evaluated in bone marrow,derived macrophage cells (BMMs) from wildtype and 4-1BB,deficient (4-1BB,/,) mice. Expression of interleukin-10 (IL-10) during osteoclast formation was analyzed at the mRNA and protein levels. Results: Expression of IL-10 was higher in RANKL-stimulated wildtype BMMs than 4-1BB,/, BMMs. When 4-1BBL was stimulated with 4-1BB,Fc fusion protein, the expression of IL-10 in BMMs increased. Neutralization of IL-10 was not as effective in preventing inhibition by IL-10 of osteoclast differentiation in 4-1BB,/, BMMs as in wildtype BMMs. When IL-10 was added to the culture medium, osteoclast formation was inhibited more efficiently in the 4-1BB,/, BMMs than in the wildtype BMMs. Conclusions: Interaction of 4-1BB and 4-1BBL stimulates IL-10 production through 4-1BBL signaling. 4-1BBL plays a role at a certain stage of osteoclast formation, and IL-10 may mediate this effect. The elevated level of osteoclastogenesis in 4-1BB,/, BMMs may thus be caused, in part, by a lower level of IL-10. [source] Television's Interpellation: Heeding, Missing, Ignoring, and Resisting the Call for Pan-National Identity in the Brazilian AmazonAMERICAN ANTHROPOLOGIST, Issue 4 2009Richard Pace ABSTRACT In this article, I focus on the ways in which audiences in the Amazonian community of Gurupá respond to television's interpellation for pan-national identity. I examine how viewers heed, miss, ignore, and resist the call for identity as well as how their various responses to this "call" shape their worldview and behavior and impact the process of nation building. Utilizing audience ethnography over a 25-year period, I show in this study how televisual messages are contextualized and localized, mitigating the forces of nationalistic homogenization. [source] Response to Galvanic Vestibular Stimulation in Patients with Unilateral Vestibular LossTHE LARYNGOSCOPE, Issue 1 2006Hyun Jik Kim MD Abstract Objectives: This study sought to characterize various responses to galvanic vestibular stimulation (GVS) by comparing GVS-induced eye movements in healthy subjects and patients with vestibular function loss. The study also aimed to estimate the clinical significance of GVS tests. Finally, an effort was made to localize the primary excitation site of stimulation in the vestibular system. Materials and Methods: Three parameters of response to GVS, spontaneous nystagmus, galvanic stimulating nystagmus (GSN), and postgalvanic stimulating nystagmus (PGSN), were evaluated in 20 normal subjects and 14 patients with complete unilateral vestibular function loss resulting from labyrinthectomy or vestibular neurectomy using a three-dimensional video-electronystagmography technique. Results: In normal subjects, GSN was detected in all subjects and was directed toward the negative electrode. PGSN was also detected but was directed toward the opposite electrode. When the negative electrode was attached to the intact side in unilateral vestibular loss subjects, GSN was always directed toward the negative electrode and PGSN was never observed. When the negative electrode was attached to the lesion side, however, GSN was detected in only one case, and PGSN was observed and directed to the intact side in 13 patients. Conclusions: The response to GVS in vestibular loss patients differed from that in normal subjects, which suggests that GVS could be useful for estimating the extent of vestibular function loss. The fact that the patterns of GVS response differed so significantly suggests that the primary site of excitation is not central but is instead the peripheral vestibular organ. [source] Testing alternative models for the conservation of koalas in fragmented rural,urban landscapesAUSTRAL ECOLOGY, Issue 4 2006CLIVE A. MCALPINE Abstract Predicting the various responses of different species to changes in landscape structure is a formidable challenge to landscape ecology. Based on expert knowledge and landscape ecological theory, we develop five competing a priori models for predicting the presence/absence of the Koala (Phascolarctos cinereus) in Noosa Shire, south-east Queensland (Australia). A priori predictions were nested within three levels of ecological organization: in situ (site level) habitat (<1 ha), patch level (100 ha) and landscape level (100,1000 ha). To test the models, Koala surveys and habitat surveys (n = 245) were conducted across the habitat mosaic. After taking into account tree species preferences, the patch and landscape context, and the neighbourhood effect of adjacent present sites, we applied logistic regression and hierarchical partitioning analyses to rank the alternative models and the explanatory variables. The strongest support was for a multilevel model, with Koala presence best predicted by the proportion of the landscape occupied by high quality habitat, the neighbourhood effect, the mean nearest neighbour distance between forest patches, the density of forest patches and the density of sealed roads. When tested against independent data (n = 105) using a receiver operator characteristic curve, the multilevel model performed moderately well. The study is consistent with recent assertions that habitat loss is the major driver of population decline, however, landscape configuration and roads have an important effect that needs to be incorporated into Koala conservation strategies. [source] Isolation of p53-target genes and their functional analysisCANCER SCIENCE, Issue 1 2004Yusuke Nakamura Mutations of the p53 gene are the most common genetic alterations found in human cancers, and are known to play crucial roles in tumor development and progression. The p53 gene encodes a protein functioning as a transcription factor, and the biological functions of p53 are manifested through the activities of its downstream genes. Identification of these downstream genes involved in the p53-signaling pathway should provide more detailed insight into the molecular mechanisms that mediate tumor-suppressor activities, as well as various responses to cellular stress. We have been attempting to isolate p53-target genes by means of various approaches, including differential display, cDNA microarray analysis, and direct cloning of the p53-binding sequences from human genomic DNA. Here I review our recent work on isolation of p53-target genes and their functional analysis. The physiological functions of p53-target genes include apoptosis (GML, p53AIP1, and STAG1), DNA repair (p53R2), inhibition of angiogenesis (BAI1), re-entry into the cell cycle (p53RFP), oxidative stress (CSR), and determination of cell fate (p53RDL1). (Cancer Sci 2004; 95: 7,11) [source] Testing Measurement Invariance Using Item Response Theory in Longitudinal Data: An IntroductionCHILD DEVELOPMENT PERSPECTIVES, Issue 1 2010Roger E. Millsap Abstract, Item response theory (IRT) consists of a set of mathematical models for the probabilities of various responses to test items as a function of item and person characteristics. In longitudinal data, changes in measured variables can only be interpreted if important psychometric features of the measured variables are assumed invariant across time. Measurement invariance is invariance in the relation of a measure to the latent variable underlying it. Measurement invariance in longitudinal studies concerns invariance over time, and IRT provides a useful approach to investigating longitudinal measurement invariance. Commonly used IRT models are described, along with the representation of measurement invariance in IRT. The use of IRT for investigating invariance is then described, along with practical considerations in using IRT for this purpose. Conceptual issues, rather than technical details, are emphasized throughout. [source] | ||||||||||