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Selected AbstractsArtificial neural network inversion of magnetotelluric data in terms of three-dimensional earth macroparametersGEOPHYSICAL JOURNAL INTERNATIONAL, Issue 1 2000Vjacheslav Spichak The possibility of solving the three-dimensional (3-D) inverse problem of geoelectrics using the artificial neural network (ANN) approach is investigated. The properties of a supervised ANN based on the back-propagation scheme with three layers of neurons are studied, and the ANN architecture is adjusted. A model class consisting of a dipping dyke in the basement of a two-layer earth with the dyke in contact with the overburden is used for numerical experiments. Six macroparameters of the 3-D model, namely the thickness of the top layer, which coincides with the depth of the dyke (D), the conductivity ratio between the first and second layers (C1,/C2,), the conductivity contrast of the dyke (C/C2,), and the width (W ), length (L ) and dip angle of the dyke (A), are used. Various groups of magnetotelluric field components and their transformations are studied in order to estimate the effect of the data type used on the ANN recognition ability. It is found that use of only the xy - and yx -components of impedance phases results in reasonable recognition errors for all unknown parameters (D: 0.02 per cent, C1/C2: 8.4 per cent, C/C2: 26.8 per cent, W : 0.02 per cent, L : 0.02 per cent, A: 0.24 per cent). The influence of the size and shape of the training data pool (including the ,gaps in education' and ,no target' effects) on the recognition properties is studied. Results from numerous ANN tests demonstrate that the ANN possesses good enough interpolation and extrapolation abilities if the training data pool contains a sufficient number of representative data sets. The effect of noise is estimated by means of mixing the synthetic data with 30, 50 and 100 per cent Gaussian noise. The unusual behaviour of the recognition errors for some of the model parameters when the data become more noisy (in particular, the fact that an increase in error is followed by a decrease) indicates that the use of standard techniques of noise reduction may give an opposite result, so the development of a special noise treatment methodology is required. Thus, it is shown that ANN-based recognition can be successfully used for inversion if the data correspond to the model class familiar to the ANN. No initial guess regarding the parameters of the 3-D target or 1-D layering is required. The ability of the ANN to teach itself using real geophysical (not only electromagnetic) data measured at a given location over a sufficiently long period means that there is the potential to use this approach for interpreting monitoring data. [source] From "Veshyas" to "Entertainment Workers": Evolving Discourses of Bodies, Rights, and Prostitution in IndiaASIAN POLITICS AND POLICY, Issue 2 2009Subir K. Kole The discursive terrain of prostitution has undergone several changes with modernity/postmodernity. Various groups of feminists hold contentious, often conflicting, ideologies on this issue. Two broad groups emerge from these debates: One takes a clear abolitionist perspective, while the other takes a sex work position. Both these groups actively lobby and join forces with individuals and institutions to influence global and national policy-making. There is a great degree of variation and overlap within and across each camp. Among those taking a sex work position, some argue that selling sex is equal to using any other part of the body for making a living. This article examines the discursive terrain of prostitution in India, focusing on what it means to treat sex as any other use of the body in commerce. It concludes that prohibition is a prejudice that India must overcome to develop sound public health policies. [source] Atlantic Forest Butterflies: Indicators for Landscape Conservation,BIOTROPICA, Issue 4b 2000Keith S. Brown Jr. ABSTRACT The Atlantic Forest region (wide sense) includes very complex tropical environments, increasingly threatened by extensive anthropogenic conversion (>90%). Ecologically specialized, short-generation insects (butterflies) are evaluated here as indicators for monitoring community richness, landscape integrity, and sustainable resource use in the region. The > 2100 butterfly species in the Atlantic Forest tegion have been censused in many sites over 35 years, giving comparable daily, weekly, monthly, and long-tetm site lists. The 21 most thoroughly studied sites include 218,914 species, of which half can be censused in a week or less. The butterfly communities are divided into six relatively distinct faunal regions, centered in the northeast, the central coastal tablelands, the southeast coastal plain, the mountains plus interior of the southeastern states, the central plateau, and the southern states. Species richness shows the highest values in coastal mountains from 15 to 23°S. Local butterfly communities show a high turnover, with 20 to 40 percent of the species, especially small Lycaenidae and Hesperiidae, recorded only as unstable populations or "tourists." Easily sampled species in the family Nymphalidae, and especially its bait-attracted subfamilies, are best correlated with the entire butterfly fauna and can be used as surrogates for species diversity. In most butterfly groups, species richness is well predicted by landscape connectivity alone, or by composite indices of environmental heterogeneity, natural disturbance, and (negatively) anthropogenic disturbance. Principal components and redundancy analyses showed that the richness and proportions of different butterfly groups in the local fauna are variably explained by disturbance, seasonality, temperature, vegetation, soils, and landscape connectivity. Various groups thus can be used as rapid indicators of different types of change in the community, its environment, and the landscape. Threatened and rare species also can be used as indicators of the most unique Atlantic Forest communities (paleoenvironments), which need special attention. RESUMO A região da Mata Atlantica latu senstt inclui ambientes tropicals muito complexes, cada vez mais amea¸ados por extensa conversão antrópica (>90%). Insetos pequenos, especializados, e de ciclo rápido (borboletas) são avaliados neste trabalho como indicadores para o monitoramento da tiqueza de comunidades, integridade de paisagens, e uso susten-tável de recursos na região. As >2100 espécies de borboletas na região da Mata Atlantica têrn sido recenseadas em muitos sítios durante os últimos 35 anos, dando listas comparáveis diárias, semanais, mensais e totais para cada sítio. Os 21 sítios mais intensivamente estudados incluem 218,914 espécies, das quais metade pode ser amostrada em uma semana ou menos. As comunidades de borboletas são divididas em seis subregiões faunísticas relativamente distintas, centradas no nordeste, nos tabuleiros baianos, no literal do sudeste, nas regiões montanhosas no interior dos estados do sudeste, no Planalto Central, e no estados do sul. A riqueza de espécies é maior nas serras costeiras entre 15 c 23°S. As comunidades locals de borboletas possuem alta reposi¸ão, com 20 a 40 por cento das espécies, especialmente os pequenos Lycaenidae e Hesperiidae, registradas em popula¸ões instáveis ou sendo apenas "turistas." As espécies facilmente amostradas na família Nymphalidae, especialmente as atraídas a iscas fermentadas, são mais correlacionadas com a riqueza total e podem ser usadas como estimadores da riqueza total no ambiente. Na maior parte dos grupos de borboletas, a riqueza de espécies é altamente correlacionada com conectividade simples da paisagem, e com índices compostos de heterogeneidade, perturba¸ão natural, e (negativamente) perturba¸ão total no ambiente. As análises de Componentes Principals e de Redundãncia mostram que as riquezas e proor¸ões de diferentes grupos de borboletas são variavelmente explicadas por pertba¸ão, sazonalidade, temperatura, vegeta¸ão, solos, e conectividade. Váries grupos podem assim ser úteis como indicadores rápidos de diferentes tipos de mudan¸as na comunidade, no seu ambiente, e na paisagem. Espécies raras e amea¸adas podem também ser usadas para indicar os sistemas mais únicos na região (paleoarnbientes), que necessitam de aten¸ão especial. [source] The effect of prenatal hypoxia on brain development: short- and long-term consequences demonstrated in rodent modelsDEVELOPMENTAL SCIENCE, Issue 4 2006Hava Golan Hypoxia (H) and hypoxia-ischemia (HI) are major causes of foetal brain damage with long-lasting behavioral implications. The effect of hypoxia has been widely studied in human and a variety of animal models. In the present review, we summarize the latest studies testing the behavioral outcomes following prenatal hypoxia/hypoxia-ischemia in rodent models. Delayed development of sensory and motor reflexes during the first postnatal month of rodent life was observed by various groups. Impairment of motor function, learning and memory was evident in the adult animals. Activation of the signaling leading to cell death was detected as early as three hours following H/HI. An increase in the counts of apoptotic cells appeared approximately three days after the insult and peaked about seven days later. Around 14,20 days following the H/HI, the amount of cell death observed in the tissue returned to its basal levels and cell loss was apparent in the brain tissue. The study of the molecular mechanism leading to brain damage in animal models following prenatal hypoxia adds valuable insight to our knowledge of the central events that account for the morphological and functional outcomes. This understanding provides the starting point for the development and improvement of efficient treatment and intervention strategies. [source] A methane-driven microbial food web in a wetland rice soilENVIRONMENTAL MICROBIOLOGY, Issue 12 2007Jun Murase Summary Methane oxidation is a key process controlling methane emission from anoxic habitats into the atmosphere. Methanotrophs, responsible for aerobic methane oxidation, do not only oxidize but also assimilate methane. Once assimilated, methane carbon may be utilized by other organisms. Here we report on a microbial food web in a rice field soil driven by methane. A thin layer of water-saturated rice field soil was incubated under opposing gradients of oxygen and 13C-labelled methane. Bacterial and eukaryotic communities incorporating methane carbon were analysed by RNA-stable isotope probing (SIP). Terminal restriction fragment length polymorphism (T-RFLP) and cloning showed that methanotrophs were the most prominent group of bacteria incorporating methane carbon. In addition, a few Myxobacteria -related sequences were obtained from the ,heavy' rRNA fraction. Denaturing gradient gel electrophoresis (DGGE) targeting 18S rRNA detected various groups of protists in the ,heavy' rRNA fraction including naked amoeba (Lobosea and Heterolobosea), ciliates (Colpodea) and flagellates (Cercozoa). Incubation of soil under different methane concentrations in air resulted in the development of distinct protozoan communities. These results suggest that methane carbon is incorporated into non-methanotrophic pro- and microeukaryotes probably via grazing, and that methane oxidation is a shaping force of the microeukaryotic community depending on methane availability. [source] A new group of parasporal inclusions encoded by the S-layer gene of Bacillus thuringiensisFEMS MICROBIOLOGY LETTERS, Issue 1 2008Gang Guo Abstract Bacillus thuringiensis produces various groups of active proteins, such as Cyt, Vip and Parasporin, in addition to the Cry protein. In this study we show S-layer proteins to be a new group of parasporal inclusions of B. thuringiensis. The S-layer consists of a two-dimensional lattice structure and is the outermost component of many archaeobacteria and eubacteria. The parasporal inclusion of B. thuringiensis strain CTC was found to be not a typical crystal protein encoded by the cry gene, but a proteinaceous inclusion encoded by the S-layer gene. Furthermore, the CTC-like strains (with their parasporal inclusions coded by the S-layer gene) are widely distributed and accounted for 25.4% of the B. thuringiensis strains tested. These strains constitue a new group of parasporal inclusions encoded by the S-layer gene of B. thuringiensis and shed new light on B. thuringiensis nontoxic strains. [source] Communication and Contention: The Role of Literacy in Conflicts with ,Abb,sid OfficialsHISTORY COMPASS (ELECTRONIC), Issue 5 2007Maaike Van Berkel ,Abb,sid officials of the late ninth and early tenth centuries operated in a highly bureaucratized and literate environment and they expressed their identity in terms of expertise in writing. However, in their daily business they had to communicate with all kinds of social groups, some of which had not , or only to a certain level , been introduced to writing. During the last three decades a series of groundbreaking studies appeared on the introduction and dissemination of writing in Medieval Europe. The role of literacy in Arab and Islamic societies in this period have as yet received very little, and mainly rather specialized, attention. The communication between ,Abb,sid officials and other social groups will be studied in cases of conflict and their settlements. Conflict settlement is also a field of research that recently witnessed important new insights. Studying the use of written documents in dispute settlements , a situation in which communication is of vital importance to all parties involved , forms therefore an excellent opportunity to analyse familiarity with, and confidence in, writing among the various groups within the ,Abb,sid caliphate. [source] Outsourcing HR as a competitive strategy?HUMAN RESOURCE MANAGEMENT, Issue 4 2005A literature review, an assessment of implications HR outsourcing as an organizational strategy has increased substantially over the last decade. However, this trend has attracted little academic attention regarding how outsourcing decisions are made, the manner in which these decisions are implemented, how outsourcing effectiveness is measured, and its impact on organizational performance. In this article, we provide a critical review of the reasons for, the processes involved in, and the perceived effectiveness of HR outsourcing. We investigate the implications of HR outsourcing for the role of the HR function and for the various groups of people affected by this strategy. We argue that organizations should apply both the resource-based view and institutional theory when making outsourcing decisions. © 2005 Wiley Periodicals, Inc. [source] Management strategy and HR in international mergers: choice, constraint and pragmatismHUMAN RESOURCE MANAGEMENT JOURNAL, Issue 1 2009Chris Rees The article combines consideration of the range of contextual factors that impact on management strategy and HR in the post-merger period (such as corporate structures and cultures, pressures from shareholders and regulatory and legal environments at national and international level) with an examination of the interests and power of various groups of actors within the firm. Specifically, we apply a framework which integrates the insights of market-based, institutionalist and micro-political approaches. We locate our analysis within the relevant international HRM literature, most notably recent debates concerning multinational corporation (MNC) merger dynamics. International mergers and acquisitions provide particularly useful scenarios through which to explore the interdependence between choice and constraint, illustrated here by processes of negotiation, compromise and balance across a range of issues in several case study organisations. The key areas highlighted concern: (1) the integration of HR strategies, and (2) processes of post-merger rationalisation. [source] Backpackers in the Northern Territory of Australia,motives, behaviours and satisfactionsINTERNATIONAL JOURNAL OF TOURISM RESEARCH, Issue 2 2003Asad Mohsin Abstract This paper reports the findings of a study of 475 backpackers in the Northern Territory of Australia. It finds that backpackers tend to visit many of the same locations as other tourists, and like those tourists, are attracted to the natural sights of the Territory. It is also found that the backpackers can be divided into various groups. For example, of this sample about one-third had left their previous career to travel, and about 12% had just completed studies. On the other hand about one-quarter were holidaying during periods of normal paid holiday leave. It is argued that the market is not solely comprised of ,life change' or rite de passage holidaymakers. Additionally, differences are found between national groupings. Copyright © 2003 John Wiley & Sons, Ltd. [source] The effects of carbohydrates upon the survival and reproduction of adult female Pimpla turionellae L. (Hym., Ichneumonidae)JOURNAL OF APPLIED ENTOMOLOGY, Issue 4 2001Özalp In this study the effects of 23 carbohydrates belonging to various groups upon the survival egg production and egg of female adult Pimpla turionellae L. were investigated. The best results among the carbohydrates tested was obtained with sucrose which was also employed as control. Glucose, maltose, trehalose and melezitose on the other hand showed no significant effect. The egg production was observed to be unaffected by glucose and maltose although it showed significant increase with trehalose and significant decrease with melezitose. Fructose and sorbitol caused a significant decrease in the survival of the insect. Fructose and sorbitol did not have any significant effect upon egg production whereas galactose caused a significant decrease. With the exception of galactose, no carbohydrate caused any significant effect upon egg hatching. Although they did not produce any eggs the female insects survived for 13.17, 15.13, 11.58 and 15.83 days in mannose, melibiose, raffinose and mannitol, respectively. The shortest life span was observed in arabinose followed by ,-methyl- D -glucoside, dulcitol, rhamnose, cellobiose, xylose, starch, lactose, sarbose, ribose and glycogen. [source] Pollution status of a tropical forest river using aquatic insects as indicatorsAFRICAN JOURNAL OF ECOLOGY, Issue 1 2010Tunde Ohiokhioya Thadeus Imoobe Abstract Aquatic insects inhabiting Okhuo River, in a tropical forest near Benin City, Southern Nigerian, were studied between January and December 2006 to determine the taxa composition, diversity, EPT index, relative proportions of the various groups and hence the pollution level of the waterbody. Three stations were selected and sampled monthly using the kick sampling technique. A total of 3235 individual aquatic insects belonging to 24 taxa distributed among 23 genera in six orders were collected. The insect orders occurred in the following order of dominance: Ephemeroptera > Odonata > Coleoptera > Diptera > Plecoptera > Tricoptera. Based on the diverse composition of the community dominated by organisms intolerant of organic enrichment, and the high diversity and EPT index, water quality in Okhuo River is not significantly degraded. Community composition varies seasonally, with a trend toward a declining proportion during the rainy season and increasing proportions during the dry season. Aquatic insect composition in Okhuo River compares favourably with those in similar, relatively undisturbed forest streams and rivers in Nigeria, but the diversity and proportional distribution of taxa vary considerably between streams. Résumé Les insectes aquatiques qui vivent dans la rivière Okhuo près de la ville de Benin, au sud du Nigeria, ont étéétudiés entre janvier et décembre 2006 afin de déterminer la composition des taxons, leur diversité, l'indice EPT, les proportions relatives des différents groupes et, de là, le taux de pollution de la rivière. On a sélectionné trois stations qui ont étééchantillonnées chaque mois en utilisant la technique d'échantillonnage par coups de pied. On a collecté un total de 3 235 insectes aquatiques appartenant à 24 taxons répartis entre 23 genres de six ordres. Les ordres des insectes se plaçaient dans l'ordre de dominance suivant : Ephéméroptères > Odonates > Coléoptères > Diptères > Plécoptères > Trichoptères. Si l'on se base sur la composition diversifiée de la communauté où dominent des organismes intolérants aux enrichissements organiques, et sur la grande diversité et l'indice EPT, on peut dire que la qualité de l'eau de la rivière Okhuo n'est pas significativement dégradée. La composition de la communauté varie avec les saisons, avec une tendance à la diminution au cours de la saison des pluies et à une augmentation au cours de la saison sèche. La composition des insectes aquatiques de la rivière Okhuo se compare avantageusement avec celles des cours d'eau semblables des forêts intactes du Nigeria, mais la diversité et la distribution proportionnelle des différents taxons varient considérablement entre les cours d'eau. [source] Myogenesis and molecules, insights from zebrafish Danio rerioJOURNAL OF FISH BIOLOGY, Issue 8 2009S.-W. Chong Myogenesis is a fundamental process governing the formation of muscle in multicellular organisms. Recent studies in zebrafish Danio rerio have described the molecular events occurring during embryonic morphogenesis and have thus greatly clarified this process, helping to distinguish between the events that give rise to fast v. slow muscle. Coupled with the well-known Hedgehog signalling cascade and a wide variety of cellular processes during early development, the continual research on D. rerio slow muscle precursors has provided novel insights into their cellular behaviours in this organism. Similarly, analyses on fast muscle precursors have provided knowledge of the behaviour of a sub-set of epitheloid cells residing in the anterior domain of somites. Additionally, the findings by various groups on the roles of several molecules in somitic myogenesis have been clarified in the past year. In this study, the authors briefly review the current trends in the field of research of D. rerio trunk myogenesis. [source] Caterers' experiences and perceptions of implementing the 2006 school meal standardsJOURNAL OF HUMAN NUTRITION & DIETETICS, Issue 5 2008C.J. Weir Background:, School meal standards were introduced in 2006 (Department for Education and Skills) and caterers are expected to comply with these standards. No research has been conducted looking at the caterer's experiences and perceptions of implementing these standards. This area has 32 school meal provider organisations for 103 schools. Half the schools are with one provider and the others are, mostly, single-handed in-house operations, often managed by a local community member. The aim of the current study was to explore caterers' understanding of the school meal standards, the barriers to implementation, and proposed solutions, to inform local practice. Methods:, A combined quantitative and qualitative design was used. A questionnaire was used to assess understanding and implementation of the standards and distributed to all the catering provider organisations (n = 32), with a good response rate of 78% (n = 25). For each question about achievement of the ten food-based standards the responses were scored 0,4 where: 0 = ,Do not intend to achieve this standard'; to 4 = ,Fully achieved'. This gave a total standards achievement score for each catering provider organisation, where the minimum score that could be achieved was zero which indicated the minimum level of achievement and the maximum score which could be achieved was forty which indicated all ten standards fully achieved. Four focus group were undertaken involving 40 people who considered themselves to be in a management role within a catering provider organisation. Semi structured interviews were undertaken with 11 people (until saturation), using purposive sampling, to explore barriers and solutions to implementation of the standards in detail. Descriptive and appropriate inferential statistics (Fisher's Exact tests and independent samples t- tests) were performedon the datausingStatistical Package for Social Sciences (SPSS). The process undertaken for the qualitative analysis was thematic analysis, and used analytic hierarchy (Richie & Lewis, 2003). Approval for this study was obtained from Leeds Metropolitan University ethics committee. Results:, The qualitative discussions led to caterers' identifying two main themes. These were: support from various groups, and the roles and responsibilities caterers believed these groups had and should be performing in order to achieve successful implementation of the standards ,Think it's good someone's shaken up school meals but who is doing the actually work , it's us isn't it'.(Semi structured interview respondent medium primary provider) ,I've found it really difficult as I'm on my own at the school, totally on my own without anyone to help'. (Focus group participant primary school group). The groups that caterers identified as those who had roles and responsibilities and who should be providing support were the ,whole school'; catering provider organisations; parents; the local authority; and, the broader environment/whole population. Caterers felt the standards had ,gone too far too soon' and did not allow choice. Caterers felt finances were a barrier, and that training was required across all sectors to achieve success. The standards implementation achievement score were statistically higher for caterers who had received formal training compared with those with only food and hygiene (P = 0.001); and, between caterers who provided to a secondary school as opposed to a primary school only (P = 0.034). There was a statistically significant relationship between providers and qualifications with those providing to secondary schools more likely to have had formal qualifications (P = 0.015). Discussion:, Caterers felt all those involved in schools and school meals needed to undertake their roles and responsibilities, to provide support, and, to implement the ethos of a whole school approach. The caterers in this study identified many barriers and practical obstacles either experienced or perceived to implementing the new school meals standards. Conclusions:, The results will be used to inform the Local Authority and Primary Care Trust to ensure the effective implementation of the school meal standards. There may be opportunity to transfer these results to other school caterers, and to develop support and training to assist implementation. [source] Girls and guys, ghetto and bougie: Metapragmatics, ideology and the management of social identities1JOURNAL OF SOCIOLINGUISTICS, Issue 5 2006David West Brown This case study explores the metapragmatic awareness of a young, academically successful, African American, female speaker. It describes some of the identities and orientations that the speaker performs through language and the perceived role of linguistic style in such performances. This study suggests that these linguistic performances are a complex negotiation of ethnicity, gender and class that both draw from and resist the macrosocial indexing of social categories. Further, the understood role of language in the social negotiations of the speaker serves as an illustration of the relationship among metapragmatics, ideology and identity and also highlights the dynamism of identity management as individuals position themselves in allegiance with, or opposition to, various groups that populate their social landscape. [source] Prospective Clinical Evaluation of Serum Cardiac Troponin T in Dogs Admitted to a Veterinary Teaching HospitalJOURNAL OF VETERINARY INTERNAL MEDICINE, Issue 5 2002Teresa C. DeFrancesco The purpose of this study was to measure serum cardiac troponin T (cTnT) with a commercially available human enzyme-linked immunoassay (ELISA) test in various groups of dogs, including those undergoing doxorubicin chemotherapy. Serum samples were obtained from 6 groups of dogs: (1) normal adult dogs (n = 15); (2) dogs with asymptomatic dilated cardiomyopathy (n = 5); (3) dogs with congestive heart failure (n = 10); (4) dogs with untreated neoplasia (n = 20); (5) dogs with skeletal muscle trauma (n = 10); and (6) dogs with neoplasia receiving doxorubicin chemotherapy (n = 4). One serum sample was obtained from each of the normal dogs, those with asymptomatic cardiomyopathy, those with congestive heart failure, and those with untreated neoplasia. Serum samples were obtained serially from the dogs that were undergoing doxorubicin chemotherapy; samples were collected before doxorubicin (30 mg/m2) administration and then 1,5,7, and 14 days after administration throughout 6 cycles for a cumulative total dose of 180 mg/m2. All normal dogs, dogs with untreated neoplasia, and dogs with asymptomatic dilated cardiomyopathy had cTnT concentrations below the lower limits of detection for the assay used (<0.05 ng/mL). Detectable concentrations of cTnT were found in 3 dogs with congestive heart failure and in 2 dogs with skeletal muscle trauma. Detectable concentrations also were found in both dogs that had received 180 mg/m2 of doxorubicin. We conclude that dogs with congestive heart failure and those with skeletal muscle trauma and dogs with neoplasia receiving high-dose doxorubicin chemotherapy may have increased serum cTnT concentration, which may be suggestive of myocardial damage. [source] Quantitative detection of hepatitis B virus DNA in serum by a new rapid real-time fluorescence PCR assayJOURNAL OF VIRAL HEPATITIS, Issue 6 2001R. Jardi A sensitive and accurate HBV DNA quantification assay is essential for monitoring hepatitis B virus (HBV) replication. This study evaluated a real-time PCR method performed in the LightCyclerTM analyser for quantitative HBV DNA assay. HBV DNA results with this method were compared with those obtained using a branched-chain DNA (bDNA) solution hybridization assay. Real-time PCR was performed using two adjacent fluorescently labelled probes and primers corresponding to the HBV core gene. The same standard employed in the bDNA assay was used for calibration. Serum samples came from 193 HBV surface antigen (HBsAg)-positive patients (34 HBV e antigen (HBeAg)-positive and 93 with antibody to HBeAg (anti-HBe)), and 66 asymptomatic HBV carriers. In addition, we analysed serum samples from 8 anti-HBe-positive patients who had been receiving lamivudine treatment for more than three years. A linear standard curve was seen in the range from 103 to 108 copies/mL. In the reproducibility analysis, intra-assay coefficient of variation (CVs) at two known HBV DNA concentrations were 4% and 2% and interassay CVs were 6% and 4%. The median of serum HBV DNA by real-time PCR was 9.2 × 108 copies/mL in HBeAg-positive patients with persistently elevated alanine aminotransferase (ALT) levels, 1.3 × 107 copies/mL in anti-HBe-positive cases with persistently elevated ALT levels, 3.7 × 104 copies/mL in anti-HBe-positive patients with fluctuating ALT levels and 104 copies/mL in asymptomatic HBV carriers. The differences in HBV DNA levels among the various groups studied were statistically significant (P < 0.05). The cut-off between chronic hepatitis patients and asymptomatic carriers was found to be at a serum HBV DNA concentration of 5 × 104 copies/mL. Of the 109 serum samples with a viral load < 7.5 × 105 (negative by bDNA assay) 44 (40%) were positive by real-time PCR: 24 (56%) chronic hepatitis and 20 (33%) asymptomatic carriers. There was a positive association between HBV DNA levels determined by real-time PCR and ALT levels (P < 0.05), which was not observed with the bDNA assay for HBV DNA quantification. At 12 months of lamivudine treatment, 6 patients (75%) showed HBV DNA levels < 5 × 104 copies/mL (range < 103,2 × 103), significantly lower than at baseline. At 36 months, 2 of 8 (25%) showed HBV DNA levels persistently lower than 5 × 104 copies/mL (1.7 × 103, 6 × 103). The LightCycler quantitative real-time PCR is a practical, sensitive, reproducible single-tube assay with a wide dynamic range of detection. The assay is automatic except for DNA extraction and the running time is only 70 min. The LightCycler real-time PCR is useful for identifying different states of HBV infection and for evaluating the efficacy of viral therapy. [source] Wage Differentials between Ethnic Groups in SwitzerlandLABOUR, Issue 1 2001Augustin De Coulon This paper analyses the average wage differentials between various groups of immigrants and the Swiss workers. Classical Oaxaca-Blinder decompositions are applied to a sample of 7,494 males (whose 1,070 immigrants) interviewed for the 1995 Swiss Labour Force Survey. Education and experience before and after migration are separately considered in two different ways. We control for sample selection in the wage and salary sector. We also investigate earnings differentials between natives and immigrants who arrived in the country before the age of 6. Our main results are that the part of differentials due to difference in coefficient varies strongly with different ethnic groups considered, that education is a strong determinant of the difference in observed characteristics and that second generation immigrants are fairly well assimilated in the Swiss labour market. [source] Bone mineral density among cirrhotic patients awaiting liver transplantationLIVER TRANSPLANTATION, Issue 5 2004Rana Paramvir Sokhi Osteoporosis is an important and common complication in patients with chronic liver disease. The goal of this study was to determine the bone mineral density (BMD) in different subgroups among pretransplant cirrhotic patients. BMD of the lumbar vertebrae (L) and femoral neck (F) were obtained in 104 consecutive cirrhotic patients. Descriptive and inferential statistics were used to compare the BMD among various groups. The mean BMD in males (n = 54) and females (n = 50) at L were 1.28 ± 0.25 g/cm2 and 1.13 ± 0.20 g/cm2, respectively (P = .001); at F they were 1.03 ± 0.14 and 0.91 ± 0.17, respectively (P < .0001). Among males, BMD at L in Child-Turcotte-Pugh class B and C were 1.40 ± 0.21 and 1.13 ± 0.20, respectively (P = .001); at F they were 1.11 ± 0.10 and 0.93 ± 0.13, respectively (P < .0001). Among females, BMD at L in Child-Turcotte-Pugh class B and C were 1.27 ± 0.18 and 1.05 ± 0.16, respectively (P = .0003); at F they were 1.02 ± 0.16 and 0.83 ± 0.12, respectively (P = .001). The BMD in premenopausal females (n = 15) and postmenopausal females (n = 35) at L were 1.20 ± 0.19 and 1.11 ± 0.20, respectively (P = .15); at F they were 0.97 ± 0.17 and 0.88 ± 0.16, respectively (P = .12). The BMD in postmenopausal females on hormone replacement therapy (n = 19) and on no hormone replacement therapy (n = 16) at L were 1.07 ± 0.17 and 1.14 ± 0.23, respectively (P = .29); at F they were 0.85 ± 0.15 and 0.91 ± 0.18, respectively (P = .33). The BMD values between etiologic groups were not significantly different. The overall prevalence of osteopenia and osteoporosis were 34.6% and 11.5%, respectively, being significantly higher in females than in males. In conclusion, significant difference in BMD values exists between males and females, as well as between Child-Turcotte-Pugh class B and C patients with cirrhosis. In addition, there is no significant influence of menopausal status, hormone replacement therapy, and etiology of cirrhosis on BMD. (Liver Transpl 2004;10:648,653.) [source] 1H chemical shifts in NMR: Part 23,, the effect of dimethyl sulphoxide versus chloroform solvent on 1H chemical shiftsMAGNETIC RESONANCE IN CHEMISTRY, Issue 5 2006Raymond J. Abraham Abstract The 1H chemical shifts of 124 compounds containing a variety of functional groups have been recorded in CDCl3 and DMSO- d6 (henceforth DMSO) solvents. The 1H solvent shift ,, = ,(DMSO) , ,(CDCl3) varies from ,0.3 to +4.6 ppm. This solvent shift can be accurately predicted (rms error 0.05 ppm) using the charge model of ,, ,, , and long-range contributions. The labile protons of alcohols, acids, amines and amides give both, the largest solvent shifts and the largest errors. The contributions for the various groups are tabulated and it is shown that for H.C.C.X ,-effects (X = OH, NH, O, NH.CO) there is a dihedral angle dependence of the ,-effect. The group contributions are discussed in terms of the possible solvent,solute interactions. For protic hydrogens, hydrogen bonding is the dominant interaction, but for the remaining protons solvent anisotropy and electric field effects appear to be the major factors. Copyright © 2006 John Wiley & Sons, Ltd. [source] DNA barcoding of Neotropical bats: species identification and discovery within GuyanaMOLECULAR ECOLOGY RESOURCES, Issue 2 2007ELIZABETH L. CLARE Abstract Sequence diversity in the cytochrome c oxidase subunit 1 gene has been shown to be an effective tool for species identification and discovery in various groups of animals, but has not been extensively tested in mammals. We address this gap by examining the performance of DNA barcodes in the discrimination of 87 species of bats from Guyana. Eighty-one of these species showed both low intraspecific variation (mean = 0.60%), and clear sequence divergence from their congeners (mean = 7.80%), while the other six showed deeply divergent intraspecific lineages suggesting that they represent species complexes. Although further work is needed to examine patterns of sequence diversity at a broader geographical scale, the present study validates the effectiveness of barcoding for the identification of regional bat assemblages, even highly diverse tropical faunas. [source] The Politics of Peace in the GDR: The Independent Peace Movement, the Church, and the Origins of the East German OppositionPEACE & CHANGE, Issue 3 2001Steven Pfaff Comparative research offers some insights into the genesis of movements under highly repressive conditions in which dissident groups are systematically denied the organizational and political resources necessary to mount a sustained challenge to the state. During the 1970s and 1980s there were circles of dissidents in the German Democratic Republic (GDR, or East Germany), but most grievances were not expressed in an organized form, and there were few opportunities to mobilize protest against the Communist regime. State repression and party control of society meant that opposition had to be organized within institutions that were shielded from state control. Religious subcultures offered a rival set of identities and values while generally accommodating the demands of the regime. Within the free social space offered by the church, a peace movement developed during the late 1970s and early 1980s. The effort to build an independent citizens' peace movement based in the church played an important role in linking together various groups committed to nonviolent protest, peace, ecology, and human rights into a coherent, if still organizationally weak, opposition during the East German revolution of 1989. [source] Toxicity of azinphos-methyl to various development stages of the codling moth Cydia pomonella (Lepidoptera: Tortricidae)PEST MANAGEMENT SCIENCE (FORMERLY: PESTICIDE SCIENCE), Issue 2 2007Haim Reuveny Abstract The response to azinphos-methyl of different life-stages of the codling moth Cydia pomonella (L.) was studied. A similarity in response to azinphos-methyl (LC50, LC90) was observed in neonate larvae obtained from the first and second generations of field populations. Mortality rates for neonate larvae of a field population cross-bred with a laboratory colony were lower (by a factor of 1.2,2.2) in comparison with field neonate larvae. The mortality rate of larvae from a laboratory colony exposed to artificial diet containing azinphos-methyl gradually decreased at older instars. The mortality rates of fifth-instar larvae were remarkably low when exposed to artificial diet mixed with azinphos-methyl or when topically treated with the insecticide. One- to three-day-old females were more sensitive than males of the same age, whereas the opposite was observed in 14,to 17-day-old adults. Mortality rates of 14- to 17-day-old adults were higher than those under 10 days old. No significant difference in sensitivity to the organophosphorus compound was noticed between the sexes of 7- to 10-day-old adults. Neonate larvae of the codling moth can serve as a target life-stage for various groups of pesticides, and the importance of using such a stage as a standardized methodology for monitoring resistance in the codling moth is discussed. Copyright © 2006 Society of Chemical Industry [source] Erwinia amylovora modifies phenolic profiles of susceptible and resistant apple through its type III secretion systemPHYSIOLOGIA PLANTARUM, Issue 3 2008Isabelle Pontais Fire blight is a disease affecting Maloideae caused by the necrogenic bacterium Erwinia amylovora, which requires the type III protein secretion system (TTSS) for pathogenicity. Profiles of methanol-extractable leaf phenolics of two apple (Malus × domestica) genotypes with contrasting susceptibility to this disease were analyzed by HPLC after infection. Some qualitative differences were recorded between the constitutive compositions of the two genotypes but in both of them dihydrochalcones accounted for more than 90% of total phenolics. Principal component analysis separated leaves inoculated with a virulent wild-type strain from those inoculated with a non-pathogenic TTSS-defective mutant or with water. The changes in levels of the various groups of phenolics in response to the virulent bacterium were similar between the two genotypes, with a significant decrease of dihydrochalcones and a significant increase of hydroxycinnamate derivatives. Differences between genotypes were, however, recorded in amplitude and kinetic of variation in these groups. Occurrence of oxidation and polymerization reactions is proposed, based on the browning process of infected tissues, but whether some by-products act in defense as toxic compounds remain to be tested. Among direct antibacterial constitutive compounds present in apple leaves, the dihydrochalcone phloretin only was found at levels close to lethal concentrations in both genotypes. However, E. amylovora exhibited the ability to stabilize this compound at sublethal levels even in the resistant apple, rejecting the hypothesis of its involvement in the resistance of this genotype. [source] Race, Gender, and Communications in Natural DisastersPOLICY STUDIES JOURNAL, Issue 4 2007Darrell M. West We examine public attitudes toward vulnerability and evacuation in hurricane natural disasters. Using the results of an opinion survey in a coastal, New England state, we find important differences in how men and women, and Whites and minorities perceive natural disasters. Race, gender, and geographic proximity to the coast affect how vulnerable people believe their residence is to a major hurricane, while government officials and media reporting telling people to evacuate influence evacuation decisions. In order to avoid future breakdowns, governments need to understand the different information processing approaches of various groups of people. [source] Prevalence of respiratory symptoms among wheat flour mill workers in Ibadan, NigeriaAMERICAN JOURNAL OF INDUSTRIAL MEDICINE, Issue 3 2004FWACP, K.T. Ijadunola MBChB Abstract Background While investigations into occupational health problems of various groups of workers have been conducted in Nigeria, so far, very little attention has been paid to the health status of workers in the grain industry. The prevalence of respiratory symptoms among wheat flour mill workers and control groups in a medium size industrial setting in Nigeria was studied. Methods The study employed a cross-sectional analytical design. Data were collected using structured interviews, work-site observations, and physical examination. Respondents consisted of 91 flour-millers, 30 matched internal controls from the maintenance unit of the same flour mill factory, and 121 matched external controls. Results Fifty-four percent of the flour-millers reported at least one respiratory symptom compared with 30% of the internal controls (P,<,0.05) and 19% of the external controls (P,<,0.001). Most symptoms were significantly more prevalent among the flour-millers compared with control subjects, and this trend was more evident amongst non-smokers than ex-smokers. Conclusions The study concluded that wheat flour mill workers in Nigeria, like grain workers elsewhere, were at an increased risk of developing both pulmonary and non-pulmonary symptoms compared with control subjects. The result has implications for improved dust control measures in the grain industry in Nigeria. Am. J. Ind. Med. 45:251,259, 2004. © 2004 Wiley-Liss, Inc. [source] First and second-trimester biochemical markers of chromosomal anomalies and their relationship to maternal haemoglobin levelsPRENATAL DIAGNOSIS, Issue 8 2005N. J. Cowans Abstract Objective To evaluate a previous hypothesis that maternal serum biochemical markers used in the assessment of Down syndrome risk are related to maternal haemoglobin concentrations. Methods A series of 1306 second-trimester prenatal screening records were retrieved including information on marker levels (AFP and f,hCG MoMs), Down's risk, a priori age risk, maternal weight and maternal height. Each individual record was merged with data from haematological investigations on samples collected on the same day. A similar series of 1688 first-trimester screening records were also retrieved including the maker levels for PAPP-A, and f,hCG MoMs were merged with data from haematological investigations carried out on the same day. The two groups were categorised according to their haemoglobin levels; anaemic (less than 11.0 g/dL in first trimester and 10.5 g/dL in the second trimester), high haemoglobin (greater than 14.0 g/dL and 13.2 g/dL) or normal (between these ranges). An analysis was made of marker levels in the various groups before and after correction for ethnicity and of the screen-positive rate in the various groups. Using a formula based on maternal height and weight, variation of marker levels with plasma volume was assessed. Results In the first trimester, 12.6% of the pregnant population was anaemic and 1.6% had elevated haemoglobin levels. In the second trimester this was 12.7 and 3.9%. These figures varied considerably with ethnic origin, with Asian and Afro-Caribbean women being more anaemic than Caucasian women. Haemoglobin levels declined by 7% between the 11- and 21-week period. Maternal plasma volume (as calculated by a widely used maternal height and weight relationship) was not correlated with weight-corrected biochemical marker MoMs in either trimester. A weak but significant correlation of maternal plasma volume and haemoglobin concentration was observed. There was no significant correlation between biochemical marker MoMs and haemoglobin concentration. Although the proportion of pregnancies designated screen positive decreased as haemoglobin levels increased, this was paralleled by a decrease in the maternal age apriori risk. Conclusions There is no relationship between maternal haemoglobin levels and the levels of Down syndrome markers in either the first or second trimester. Biochemical marker levels do not need to be corrected for haemoglobin concentrations when used in screening for Down syndrome. Copyright © 2005 John Wiley & Sons, Ltd. [source] Relatedness in wild chimpanzees: Influence of paternity, male philopatry, and demographic factorsAMERICAN JOURNAL OF PHYSICAL ANTHROPOLOGY, Issue 3 2008Eiji Inoue Abstract In chimpanzees (Pan troglodytes), high-ranking males are expected to have high reproductive success and females typically emigrate upon reaching maturity. Although high average relatedness among males in the same social groups has been assumed, previous reports have indicated that relatedness among males is not necessarily significantly higher than that among females. The paternity of 11 offspring and the relatedness of 50 individuals in the M group of chimpanzees at Mahale Mountains National Park, Tanzania, were investigated using DNA analyses. We determined the fathers of 10 offspring. Two different alpha males sired a total of five offspring, whereas the other males had low reproductive success. The proportion of paternal half-sibling pairs among the 10 offspring was 15.6%. The average relatedness among mature males was significantly higher than that among mature females. The existence of an old male and the long tenure of one alpha male may have contributed to this significant difference. The average dyadic relatedness among mature natal individuals was significantly higher than that in natal-immigrant pairs in which the individuals came from different groups. The average relatedness among immigrant females was similar to that in pairs of natal and immigrant females, suggesting that the immigrants came from various groups. Thus, female transfer acts to maintain low average relatedness within the group. A comparison of our results to those from other study sites suggests that although the average relatedness among adult males does not reach the level of half-siblings, under some circumstances it can exceed the relatedness of females. Am J Phys Anthropol, 2008. © 2008 Wiley-Liss, Inc. [source] ORIGINAL ARTICLE: The Role of Cytokine Expression in Different Subgroups of Subfertile MenAMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, Issue 5 2009Srividya Seshadri Problem, The aim of this study was to evaluate the levels of seminal plasma cytokines interleukin 6 (IL-6), interleukin 8 (IL-8), interleukin 10 (IL-10), interleukin 11 (IL-11), interleukin 12 (IL-12), tumour necrosis factor alpha (TNF-,) and interferon gamma (IFN-,) in male subfertility. Method of study, A total of 73 male partners of an infertile couple attending a regional andrology unit were recruited into this prospective study and subdivided into the various groups based on semen analysis. Concentrations of cytokines such as IL-6, IL-8, IL-10, IL-11, IL-12, TNF-, and IFN-, in the seminal plasma were determined using enzyme linked immunosorbent assay (ELISA). Results, Significant higher concentrations (P < 0.05) of IL-6 in the mild and severe oligospermic group, IL-8 and IL-10 in the asthenospermic group and IL-6, IL-10, TNF-, and IFN-, in the obstructed azoospermic group were determined. IL-10 concentrations correlated significantly with other cytokines in the obstructed azoospermic group and the asthenospermic group. Conclusion, Our study confirms that cytokines rarely act in isolation, but rather in a network of other cytokines and may affect sperm function directly or indirectly. The presence of increased levels of cytokines in the obstructed azoospermic group suggests that the cytokines may not originate from the testis. [source] DETECTING CHANGES OF CELTIC GOLD SOURCES THROUGH THE APPLICATION OF TRACE ELEMENT AND Pb ISOTOPE LASER ABLATION ANALYSIS OF CELTIC GOLD COINS*ARCHAEOMETRY, Issue 4 2009C. BENDALL This project follows on from an initial study of Celtic gold coins from the Middle Rhine/Moselle region, which was based on material found at the Martberg, a Late Iron Age/Roman sanctuary and settlement (River Moselle, western Germany; Bendall 2003). The earlier work was expanded to encompass over 100 examples of various other regional Celtic gold coinages from the collection of the Römisch-Germanisches Zentralmuseum, Mainz. The alloy (Au,Ag,Cu) and trace element compositions (in particular Ni, Sb and Pt) were determined by EPMA and LA,ICP,MS, respectively, and their Pb isotope signatures were measured by LA,MC,ICP,MS. Of the 28 trace elements measured, only Ni, Sb and Pt were found to show meaningful variations and so only they are presented here. In particular, differences in the Pt/Au ratios between various groups of earlier coinage (imitations of Philippus and Alexander/Nike staters, Sch. 23 and some early Boian coins) on the one hand, and the majority of the Boian and the southern rainbow cup coinages on the other, indicate a significant difference in the gold sources exploited for these regional coinages. The Pb isotope data confirm previous conclusions that the contribution of gold to the total lead in the Au,Ag,Cu coin alloys can be detected, especially for coins with over 70% gold, and show that possible gold sources include both eastern Mediterranean and Alpine sources. Combining the Pb isotope data with the Pt/Au ratios allows the potential gold sources to be further differentiated. [source] | ||||||||||||||||||||||||||||