UA Concentration (ua + concentration)

Distribution by Scientific Domains

Selected Abstracts

Assessment of endothelial function and blood metabolite status following acute ingestion of a fructose-containing beverage

A. J. Bidwell
Abstract Aim:, Fructose intake has increased concurrent with sugar intake and this increase has been implicated in contributing to the development of metabolic syndrome risk factors. Recent evidence suggests a role for uric acid (UA) as a potential mediator via suppression of nitric oxide (NO) bioavailability. The aim of this study was to explore this hypothesis by measuring changes in UA concentration and systemic NO bioavailability as well as endothelial function in response to acute ingestion of a glucose-fructose beverage. Methods:, Ten young (26.80 ± 4.80 years), non-obese (body mass index: 25.1 ± 2.55 kg m,2; percent body fat: 13.5 ± 6.9%) male subjects ingested either a glucose (100 g dextrose in 300 mL) or isocaloric glucose-fructose (glucose : fructose; 45 : 55 g in 300 mL) beverage. Blood was sampled pre- and every 15-min post-ingestion per 90 min and assayed for glucose, lactate, fructose, total nitrate/nitrate, UA and blood lipids. Forearm blood flow and pulse-wave velocity were recorded prior to and at 30 and 45 min time intervals post-ingestion, respectively, while heart rate, systolic and diastolic blood pressure were recorded every 15 min. Results:, The glucose-fructose ingestion was associated with a significant (P < 0.05) increase in plasma lactate concentration and altered free fatty acid levels when compared with glucose-only ingestion. However, UA was not significantly different (P = 0.08) between conditions (AUC: ,1018 ± 1675 vs. 2171 ± 1270 ,mol L,1 per 90 min for glucose and glucose-fructose conditions respectively). Consequently, no significant (P < 0.05) difference in endothelial function or systemic NO bioavailability was observed. Conclusion:, Acute consumption of a fructose-containing beverage was not associated with significantly altered UA concentration, endothelial function or systemic NO bioavailability. [source]

A Selective Voltammetric Method for Uric Acid Detection at a Glassy Carbon Electrode Modified with Electrodeposited Film Containing DNA and Pt-Fe(III) Nanocomposites

Shuqing Wang
Abstract A novel biosensor by electrochemical codeposited Pt-Fe(III) nanocomposites and DNA film was constructed and applied to the detection of uric acid (UA) in the presence of high concentration of ascorbic acid (AA). Based on its strong catalytic activity toward the oxidation of UA and AA, the modified electrode resolved the overlapping voltammetric response of UA and AA into two well-defined peaks with a large anodic peak difference (,Epa) of about 380mV. The catalytic peak current obtained from differential pulse voltammetry (DPV) was linearly dependent on the UA concentration from 3.8×10,6 to 1.6×10,4,M (r=0.9967) with coexistence of 5.0×10,4,M AA. The detection limit was 1.8×10,6,M (S/N=3) and the presence of 20 times higher concentration of AA did not interfere with the determination. The modified electrode shows good sensitivity, selectivity and stability. [source]

Determination of uric acid in plasma and allantoic fluid of chicken embryos by capillary electrophoresis

Jana Mat, ková
Abstract Capillary electrophoresis with diode array detection (DAD) was used to determine uric acid (UA) in chicken plasma and the allantoic fluid of chicken embryos. Complete separation of uric and ascorbic acids was attained in less than 10 min in the optimized BGE containing 60 mM MES + 30 mM Tris + 0.001% (w/v) polybrene (pH 6.1). The limit of UA detection (0.2 mg/L) was found to be low enough for sensitive analysis of native plasma and allantoic fluid samples. Range of linearity (1,200 mg/L), repeatability for peak area (CV <4.1%) and migration time (CV <2.5%), as well as recovery of UA from biological samples (97,100%), were found to be satisfactory. The method was applied to detect the elevated UA concentrations (hyperuricemia) in chicken embryos with induced unilateral renal agenesis. CE/DAD analysis of the chicken plasma can be carried out with a relatively small volume of samples (1 ,L). [source]