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Tubular Diameter (tubular + diameter)

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Medical Sciences	100%

Selected Abstracts

Histological evidences suggest recommending orchiopexy within the first year of life for children with unilateral inguinal cryptorchid testis

INTERNATIONAL JOURNAL OF UROLOGY, Issue 7 2007
Kwan Hyun Park
Objective: To determine the optimal timing for orchiopexy, we evaluated the histological parameters of the cryptorchid testis. Methods: We prospectively performed testicular biopsy in a total of 65 consecutive children with palpable unilateral inguinal cryptorchid testes. For controls, we used testicular histological slides from 15 age-matched children with testicular tumor. To investigate the fertility potential, we analyzed the parameters including mean tubular diameter (MTD), mean tubular fertility index (MTFI), germ cell count/tubule (GCC), Sertoli cell index (SCI) and interstitial fibrosis index (IFI). Results: The MTFI and GCC in children ,1 years of age were significantly higher than those of other older age groups. The MTFI, GCC and IFI were significantly better in patients ,2 years of age when compared to those of > 2 years. Compared to the controls, the MTFI and GCC in the patients were significantly worse in those aged > 2 years at surgical repair. In the ,2-year age group, the MTFI and GCC of the cryptorchid testis showed a decreasing tendency with age, which were contrasting with the ascending curves in the control and the curves crossed at 1,2 years of age in each parameter. Conclusions: To protect fertility potential, we recommend, orchiopexy should be performed within the first year of life, and no later than 2 years of age in patients with palpable inguinal cryptorchid testes. [source]

Partial depletion of macrophages by ED7 reduces renal injury in Adriamycin nephropathy

NEPHROLOGY, Issue 5 2005
YIPING WANG
SUMMARY: Background: Because macrophages are considered to be possible effectors of disease in Adriamycin (ADR) nephrosis, we hypothesized that depletion of macrophages might protect against the initiation of renal injury. In the present study, a monoclonal antibody (ED7) directed against CD11b/CD18 integrin, which is expressed by macrophages, was used to investigate the pathogenetic effects of macrophages in ADR nephropathy. Methods: Male Wistar rats were treated with ED7 antibody, starting 1 day prior to ADR (7.5 mg/kg) treatment, or 7 days post-ADR when overt proteinuria was established. Results: Circulating ED7-positive cells were reduced by approximately 30% in rats with ADR nephrosis by the ED7 antibody, while the number of macrophages in the renal cortex of ADR rats was reduced by nearly 50% with the ED7 treatment, whether administered before or after ADR. Creatinine clearance was significantly ameliorated by ED7 when commenced pre-ADR (P < 0.05), but not when commenced post-ADR (P = NS) in comparison to untreated ADR rats. However, proteinuria was not alleviated by either ED7 treatment. Morphometric analysis showed less glomerular sclerosis when ED7 was commenced pre-ADR compared with ADR alone (P < 0.01), but not when commenced post-ADR (P = NS). Tubular atrophy was reduced by ED7 when it was commenced pre-ADR (tubular cell height and tubular diameter: P < 0.01 and P < 0.001, respectively), as was interstitial expansion (P < 0.01) compared with ADR alone. Cortical macrophage infiltration was reduced by 50% compared with ADR alone by the ED7 commenced before or after ADR. The number of cortical CD4+ T cells fell with ED7 starting pre-ADR, but not with the ED7 treatment commencing after ADR. Conclusion: Partial macrophage depletion starting before but not after ADR protected both renal function and structure in this model of chronic proteinuric renal disease. [source]

Toxic effects of dexamethasone on mouse testicular germ cells

ANDROLOGIA, Issue 4 2010
M. Orazizadeh
Summary Exposure to glucocorticoids (GCs) leads to numerous changes in various biological systems including the reproductive system. This work evaluated effects of dexamethasone (Dex), a widely used GC, on mouse testicular germ cells. Experimental groups (E1,E3) received one of the following treatments daily for 7 days: 4, 7 and 10 mg kg,1 Dex respectively. Control groups were treated with equivalent volumes of saline. Testicular histopathology, morphometric analysis and deoxy-UTP-digoxigenin nick end labeling (TUNEL) assessment were performed for evaluation of the toxic effects of Dex and detection of the apoptotic cells. The results showed that Dex induces histopathological alterations such as epithelial vacuolisation, atrophy and reduction in testicular spermatozoid. Morphometrical data showed that Dex significantly reduced tubular diameter and epithelial height (P < 0.05). Johnsen's scoring also showed poor spermatogenesis in E2 and E3 groups (P < 0.05). Apoptotic index of germ cells was significantly increased in E2 (18.9% versus 1.76%, P < 0.01) and E3 (24.6 versus 1.76%, P < 0.001) groups. It is concluded that Dex acts as testicular toxicant and that further studies are needed to establish its mechanism of action upon spermatogenesis. [source]

Fibrosis correlates with detailed histological analysis of human undescended testes

BJU INTERNATIONAL, Issue 11 2008
Anne Suskind
OBJECTIVE To evaluate whether semiquantitatively assessing the histological degree of fibrosis provides equivalent information to a detailed histological analysis of undescended testes in children. PATIENTS AND METHODS We reviewed retrospectively the histological and clinical data from 86 consecutive orchidopexies. Biopsies fixed in Bouin's solution were evaluated histologically for tubular diameter, germ cell number and fibrosis. Fibrosis was assessed semiquantitatively by a pathologist reviewing sections stained with Masson's trichrome. The median (range) age of the patients was 1.9 (0.5,13.5) years, with 29 aged <1 year and 57 aged >1 year. RESULTS There were 65 palpable and 21 impalpable testes; 62 were inguinal and 13 were intra-abdominal. Testicular biopsies showed smaller tubules and fewer germ cells in patients aged >1 year, but there were no significant differences based on the location of the testis on physical examination or at the time of operation. The amount of fibrosis correlated well with tubular diameter and germ cell counts. CONCLUSION Fibrosis can be assessed easily and correlates well with more complex forms of histological evaluation of testicular health in children. [source]