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Tracking Methods (tracking + methods)
Kinds of Tracking Methods Selected AbstractsQuantitative microbial faecal source tracking with sampling guided by hydrological catchment dynamicsENVIRONMENTAL MICROBIOLOGY, Issue 10 2008G. H. Reischer Summary The impairment of water quality by faecal pollution is a global public health concern. Microbial source tracking methods help to identify faecal sources but the few recent quantitative microbial source tracking applications disregarded catchment hydrology and pollution dynamics. This quantitative microbial source tracking study, conducted in a large karstic spring catchment potentially influenced by humans and ruminant animals, was based on a tiered sampling approach: a 31-month water quality monitoring (Monitoring) covering seasonal hydrological dynamics and an investigation of flood events (Events) as periods of the strongest pollution. The detection of a ruminant-specific and a human-specific faecal Bacteroidetes marker by quantitative real-time PCR was complemented by standard microbiological and on-line hydrological parameters. Both quantitative microbial source tracking markers were detected in spring water during Monitoring and Events, with preponderance of the ruminant-specific marker. Applying multiparametric analysis of all data allowed linking the ruminant-specific marker to general faecal pollution indicators, especially during Events. Up to 80% of the variation of faecal indicator levels during Events could be explained by ruminant-specific marker levels proving the dominance of ruminant faecal sources in the catchment. Furthermore, soil was ruled out as a source of quantitative microbial source tracking markers. This study demonstrates the applicability of quantitative microbial source tracking methods and highlights the prerequisite of considering hydrological catchment dynamics in source tracking study design. [source] Contradictory results from different methods for measuring direction of insect flightFRESHWATER BIOLOGY, Issue 10 2004Kate H. Macneale Summary 1. Stream ecologists have been puzzled by the apparent paradox that invertebrate populations persist in headwater streams despite the high frequency with which individuals drift downstream. To resolve this ,drift paradox', directions and distances of both larval and adult movement must be identified. Using over 50 interception traps in combination with results from several mark,capture experiments using 15N as a label, we tested the assumption that interception traps accurately represent the ultimate direction of adult insect flight. 2. In several streams in the Hubbard Brook Experimental Forest, 76% of 15N-labelled stoneflies (Leuctra ferruginea) had flown upstream from where they emerged to where they were captured. In contrast, over 60% of stoneflies were flying downstream when captured, i.e. on the upstream side of an interception trap. 3. The instantaneous direction, as indicated by the side of the interception trap on which they were captured, indicated the ultimate flight direction for fewer than 1/3 of the individuals captured. Thus, such traps did not accurately reflect the ultimate flight patterns of individuals, as indicated by mark,capture data. 4. Conclusions drawn from interception trap counts regarding the direction of movement and the distribution and persistence of populations may need to be re-evaluated. We suggest that better tracking methods, including mass mark,capture studies using stable isotopes, be used to evaluate the potentially complex patterns of adult insect movement and the consequences of that movement for individuals and populations. [source] Application of microbial source tracking methods in a Gulf of Mexico field settingJOURNAL OF APPLIED MICROBIOLOGY, Issue 5 2009A. Korajkic Abstract Aims:, Microbial water quality and possible human sources of faecal pollution were assessed in a Florida estuary that serves shellfishing and recreational activities. Methods and Results:, Indicator organisms (IO), including faecal coliforms, Escherichia coli and enterococci, were quantified from marine and river waters, sediments and oysters. Florida recreational water standards were infrequently exceeded (6,10% of samples); however, shellfishing standards were more frequently exceeded (28%). IO concentrations in oysters and overlaying waters were significantly correlated, but oyster and sediment IO concentrations were uncorrelated. The human-associated esp gene of Enterococcus faecium was detected in marine and fresh waters at sites with suspected human sewage contamination. Lagrangian drifters, used to determine the pathways of bacterial transport and deposition, suggested that sediment deposition from the Ochlockonee River contributes to frequent detection of esp at a Gulf of Mexico beach. Conclusions:, These data indicate that human faecal pollution affects water quality in Wakulla County and that local topography and hydrology play a role in bacterial transport and deposition. Significance and Impact of the Study:, A combination of IO enumeration, microbial source tracking methods and regional hydrological study can reliably inform regulatory agencies of IO sources, improving risk assessment and pollution mitigation in impaired waters. [source] Direct comparison of four bacterial source tracking methods and use of composite data setsJOURNAL OF APPLIED MICROBIOLOGY, Issue 2 2007E.A. Casarez Abstract Aims:, Four bacterial source tracking (BST) methods, enterobacterial repetitive intergenic consensus sequence polymerase chain reaction (ERIC-PCR), automated ribotyping using HindIII, Kirby-Bauer antibiotic resistance analysis (KB-ARA) and pulsed-field gel electrophoresis (PFGE) were directly compared using the same collection of Escherichia coli isolates. The data sets from each BST method and from composite methods were compared for library accuracy and their ability to identify water isolates. Methods and Results:, Potential sources of faecal pollution were identified by watershed sanitary surveys. Domestic sewage and faecal samples from pets, cattle, avian livestock, other nonavian livestock, avian wildlife and nonavian wildlife sources were collected for isolation of E. coli. A total of 2275 E. coli isolates from 813 source samples were screened using ERIC-PCR to exclude clones and to maximize library diversity, resulting in 883 isolates from 745 samples selected for the library. The selected isolates were further analysed using automated ribotyping with HindIII, KB-ARA and PFGE. A total of 555 E. coli isolates obtained from 412 water samples were analysed by the four BST methods. A composite data set of the four BST methods gave the highest rates of correct classification (RCCs) with the fewest unidentified isolates than any single method alone. RCCs for the four-method composite data set and a seven-way split of source classes ranged from 22% for avian livestock to 83% for domestic sewage. Two-method composite data sets were also found to be better than individual methods, having RCCs similar to the four-method composite and identification of the same major sources of faecal pollution. Conclusions:, The use of BST composite data sets may be more beneficial than the use of single methods. Significance and Impact of the Study:, This is one of the first comprehensive comparisons using composite data from several BST methods. While the four-method approach provided the most desirable BST results, the use of two-method composite data sets may yield comparable BST results while providing for cost, labour and time savings. [source] Automatic slice positioning (ASP) for passive real-time tracking of interventional devices using projection-reconstruction imaging with echo-dephasing (PRIDE)MAGNETIC RESONANCE IN MEDICINE, Issue 4 2009S. Patil Abstract A novel and fast approach for passive real-time tracking of interventional devices using paramagnetic markers, termed "projection-reconstruction imaging with echo-dephasing" (PRIDE) is presented. PRIDE is based on the acquisition of echo-dephased projections along all three physical axes. Dephasing is preferably set to 4, within each projection ensuring that background tissues do not contribute to signal formation and thus appear heavily suppressed. However, within the close vicinity of the paramagnetic marker, local gradient fields compensate for the intrinsic dephasing to form an echo. Successful localization of the paramagnetic marker with PRIDE is demonstrated in vitro and in vivo in the presence of different types of off-resonance (air/tissue interfaces, main magnetic field inhomogeneities, etc). In order to utilize the PRIDE sequence for vascular interventional applications, it was interleaved with balanced steady-state free precession (bSSFP) to provide positional updates to the imaged slice using a dedicated real-time feedback link. Active slice positioning (ASP) with PRIDE is demonstrated in vitro, requiring approximately 20 ms for the positional update to the imaging sequence, comparable to existing active tracking methods. Magn Reson Med, 2009. © 2009 Wiley-Liss, Inc. [source] Automatic passive tracking of an endorectal prostate biopsy device using phase-only cross-correlationMAGNETIC RESONANCE IN MEDICINE, Issue 5 2008André de Oliveira Abstract MR-guided transrectal prostate biopsy is currently a time-consuming procedure because the imaging slice is often manually realigned with the biopsy needle during lesion targeting. In this work a pulse sequence is presented that automatically follows a passive marker attached to a dedicated MR biopsy device holder, thus providing an alternative to existing active tracking methods. In two orthogonal tracking FLASH images of the marker the position of the needle axis is automatically identified using a phase-only cross-correlation (POCC) algorithm. The position information is then used to realign a trueFISP imaging slice in real time. In phantom experiments the sensitivity of this technique to initial misalignments of the marker and to the signal-to-noise ratio was evaluated. In several puncture experiments the precision of the needle placement was analyzed. The POCC algorithm allowed for a precise identification of the marker in the images even under severe initial misalignments of up to 45°. At a frame rate 1 image/s a precision of the needle placement of 1.5 ± 1.1 mm could be achieved. Magn Reson Med 59:1043,1050, 2008. © 2008 Wiley-Liss, Inc. [source] Comparison of secondary ion mass spectrometry and micromilling/continuous flow isotope ratio mass spectrometry techniques used to acquire intra-otolith ,18O values of wild Atlantic salmon (Salmo salar)RAPID COMMUNICATIONS IN MASS SPECTROMETRY, Issue 17 2010N. N. Hanson The chemical signals in the sequential layers of fish otoliths have the potential to provide fisheries biologists with temporal and spatial details of migration which are difficult to obtain without expensive tracking methods. Signal resolution depends, however, on the extraction technique used. We compared the use of mechanical micromilling and continuous flow isotope ratio mass spectrometry (CF-IRMS) methods with secondary ion mass spectrometry (SIMS) to obtain ,18O profiles from otoliths of wild Atlantic salmon (Salmo salar) and used these to corroborate the time of freshwater emigration of the juvenile with macroscopic patterns within the otolith. Both techniques showed the transition occurring at the same visible feature on the otolith, allowing future analyses to easily identify the juvenile (freshwater) versus adult (marine) life-stages. However, SIMS showed a rapid and abrupt transition whereas micromilling provided a less distinct signal. The number of samples that could be obtained per unit area sampled using SIMS was 2 to 3 times greater than that when using micromilling/CF-IRMS although the ,18O values and analytical precisions (,0.2,) of the two methods were comparable. In addition, SIMS ,18O results were used to compare otolith aragonite values with predicted values calculated using various isotope fractionation equations. Copyright © 2010 John Wiley & Sons, Ltd. [source] Sources of water vapour contributing to the Elbe flood in August 2002,A tagging study in a mesoscale modelTHE QUARTERLY JOURNAL OF THE ROYAL METEOROLOGICAL SOCIETY, Issue 638 2009H. Sodemann Abstract In this study we investigate the contribution of various moisture sources to the Elbe flood that occurred in Central Europe during August 2002. An 8-day simulation with the mesoscale numerical weather prediction model CHRM, including newly implemented water vapour tracers, has been performed. According to the simulation, rather than drawing moisture from one single dominant source region, water vapour from widely separated moisture sources contributed to the extreme precipitation in the most affected area, notably at distinct, subsequent periods of time, and each in significant amounts. These moisture sources include the Atlantic and Mediterranean ocean areas inside the model domain, evapotranspiration from land areas, and long-range advection from subtropical areas outside the model domain. The results highlight the importance of the concurrent upper-level circulation and the mesoscale flow structures associated with the cyclone for producing extreme precipitation in parts of Germany, Austria, and the Czech Republic during that period. Furthermore, the numerical and technical problems of implementing water vapour tracers into a limited-area model are discussed, including conservative tracer advection, initialization, boundary treatment, and the handling of precipitation parametrizations. An evaluation of the consistency of the method in terms of water vapour, cloud water, and precipitation is provided, with generally satisfying results. The model with its detailed water vapour tracer implementation can now be used for further case-studies and climatological simulations, and serve as a reference for evaluating the performance of other moisture tracking methods, such as those based on backward trajectories. Copyright © 2009 Royal Meteorological Society [source] | ||||||||||