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Time Observations (time + observation)
Selected AbstractsNanosecond pulsed electric field generators for the study of subcellular effectsBIOELECTROMAGNETICS, Issue 3 2006Juergen F. Kolb Abstract Modeling and experimental studies have shown that pulsed electric fields of nanosecond duration and megavolt per meter amplitude affect subcellular structures but do not lead to the formation of large pores in the outer membrane. This "intracellular electromanipulation" requires the use of pulse generators which provide extremely high power but low energy pulses. In this study, we describe the concept of the required pulsed power sources, their design, operation, and the necessary diagnostics. Two types of pulse generators based on the Blumlein line principle have been developed and are described here. One system is designed to treat a large number of cells in cuvettes holding volumes from 0.1 to 0.8 ml. Pulses of up to 40 kV amplitude, with a duration of 10 ns and a rise time close to 1 ns can be applied to the cuvette. For an electrode gap of 1 mm this voltage corresponds to an average electric field of 40 MV/m. The second system allows for real time observation of individual cells under a microscope. It generates pulses of 10,300 ns duration with a rise time of 3.5 ns and voltage amplitudes up to 1 kV. Connected to a microreactor with an electrode gap of 100 µm, electric fields up to 10 MV/m are applied. Bioelectromagnetics 27:172,187, 2006. © 2005 Wiley-Liss, Inc. [source] Descriptions and biological notes of Ctenoplectra bees from Southeast Asia and Taiwan (Hymenoptera: Apidae: Ctenoplectrini) with a new species from North BorneoENTOMOLOGICAL SCIENCE, Issue 3 2009I-Hsin SUNG Abstract Six Ctenoplectra species are recorded from Southeast Asia and Taiwan. They are C. chalybea Smith, C. cornuta Gribodo, C. davidi Vachal, C. elsei Engel, C. sandakana sp. nov. and C. vagans Cockerell. Females of C. sandakana sp. nov. from North Borneo are similar to the mainland species C. chalybea, but differ mainly in the clypeal keel and the length of the antennal segments. The small blackish species, C. cornuta, is distributed in Myanmar, China and Taiwan and C. davidi is distributed in China, Russia and Taiwan; both species are seen at the flowers of Thladiantha. Ctenoplectra chalybea was collected from the Malay Peninsula, Myanmar, Taiwan and Vietnam. Ctenoplectra apicalis Smith and C. kelloggi Cockerell are allied to C. chalybea; however, C. kelloggi is excluded from this study due to insufficient material. A key to the six known Ctenoplectra species is given. The large metallic species, C. chalybea and C. elsei, visit flowers of Momordica cochinchinensis (Lour.) Spreng. For the first time observations on the nest structures of C. chalybea and C. cornuta are presented. They choose remarkable places, such as artificial structures and buildings, for nest sites. The nest architecture prevents rain and direct sunlight from entering the nest. Bees used pre-existing holes or crevices in wood for nesting shelters and collected soil and appeared to mix it with some other substance to build nests. The cell lining materials and rubbing behaviors against the cell wall suggest that Ctenoplectra bees use floral oil mainly for cell lining materials. [source] Long-term precipitation and slide activity in south-eastern Norway, autumn 2000HYDROLOGICAL PROCESSES, Issue 4 2008Christian Jaedicke Abstract In autumn 2000 high amounts of precipitation caused a large number of landslides in south-eastern Norway. The precipitation reached more than 400% of the normal monthly values in November. In addition to the precipitation, groundwater monitoring showed unusual high levels. High air temperatures in the mountains caused the precipitation to fall as rain instead of snow, causing additional runoff in the catchments. In this paper, 90 landslides are presented that were studied and evaluated by the Norwegian Geotechnical Institute (NGI) in the counties effected by the slide activity. The landslide database serves as start point for an analysis of the connection between slide activity and precipitation. The analysis shows a high variability of the observed precipitation just a few days before the slides. This variability decreases significantly around 50 days prior to the slides when the results stabilize at about 400% of normal precipitation in a 50-day period. Comparison of these results to long time observations gives an estimate of return periods for the precipitation events. The 53 days accumulated precipitation has the highest return period of 132 years. This suggests that the high landslide activity in autumn 2000 in south-eastern Norway was caused by a long-term infiltration of large amounts of precipitation rather than high rainfall intensities during a short period. Copyright © 2007 John Wiley & Sons, Ltd. [source] Cellular response mechanisms in Pseudomonas aeruginosa PseA during growth in organic solventsLETTERS IN APPLIED MICROBIOLOGY, Issue 3 2009R. Gaur Abstract Aims:, Solvent-tolerant bacteria have emerged as a new class of micro-organisms able to grow at high concentrations of toxic solvents. Such bacteria and their solvent-stable enzymes are perceived to be useful for biotransformations in nonaqueous media. In the present study, the solvent-responsive features of a lipase,producing, solvent-tolerant strain Pseudomonas aeruginosa PseA have been investigated to understand the cellular mechanisms followed under solvent-rich conditions. Methods and Results:, The solvents, cyclohexane and tetradecane with differing log P -values (3·2 and 7·6 respectively), have been used as model systems. Effect of solvents on (i) the cell morphology and structure (ii) surface hydrophobicity and (iii) permeability of cell membrane have been examined using transmission electron microscopy, atomic force microscopy and other biochemical techniques. The results show that (i) less hydrophobic (low log P -value) solvent cyclohexane alters the cell membrane integrity and (ii) cells adapt to organic solvents by changing morphology, size, permeability and surface hydrophobicity. However, no such changes were observed in the cells grown in tetradecane. Conclusions:, It may be concluded that P. aeruginosa PseA responds differently to solvents of different hydrophobicities. Bacterial cell membrane is more permeable to less hydrophobic solvents that eventually accumulate in the cytoplasm, while highly hydrophobic solvents have lesser tendency to access the membrane. Significance and Impact of the Study:, To the best of our knowledge, these are first time observations that show that way of bacterial solvent adaptability depends on nature of solvent. Difference in cellular responses towards solvents of varying log P -values (hydrophobicity) might prove useful to search for a suitable solvent for carrying out whole-cell biocatalysis. [source] | ||||||||||