Home About us Contact
|
Home About us Contact | ||
|
|
||
Base Line (base + line)
Selected AbstractsTrials update in walesCYTOPATHOLOGY, Issue 2007A. Fiander Three ongoing studies will be presented and discussed. Prevalence of Human Papillomavirus Infection in a South Wales Screening population Methods: A total of 10 000 consecutive, anonymous liquid based cytology screening samples were collected over a five month period in 2004. Age, cytology result and social deprivation score was provided for each specimen. The methodology was chosen to ensure inclusion of all women attending routine cervical screening, avoiding potential constraints associated with obtaining individual informed consent. The liquid based cytology samples were processed and reported by the receiving cytology laboratory and the residual specimens sent to the HPV Research Laboratory, Wales College of Medicine, where they were processed and stored at -80°C until analysis. High risk and low risk HPV Typing was undertaken using PCR , EIA (Jacobs et al 1997). Full high risk typing was performed on HPV positive specimens. Results: The study population had a mean age of 38 years with 92% negative, 5% borderline and 3% dyskaryotic cytology. The average social deprivation score was 17.4 (based upon the Welsh Index of multiple deprivation). The following results will be presented: HPV prevalence by age. HPV prevalence by cytology result. Type specific HPV prevalence in single and multiple infection. Conclusion: This study represents the largest type specific HPV Prevalence Study in the UK to date. As such it will form a useful base line against which to access performance of marketed HPV tests and evaluating the impact following implementation of HPV vaccination. [Funded by Welsh Office for Research and Development] CRISP , 1 Study (Cervical Randomized Intervention Study Protocol -1) Background: Indole-3-carbinol (I3C) and Diindolylmethane (DIM) are found in cruciferous vegetables and have been identified as compounds that could potentially prevent or halt carcinogenesis. I3C spontaneously forms DIM in vivo during acid digestion. I3C has been shown to prevent the development of cervical cancer in HPV 16 transgenic mice and both I3C and DIM have been shown to promote cell death in cervical cancer cell models. DIM is the major active bi-product of I3C and preliminary data indicate that DIM is active in cervical dysplasia and may be better tolerated than I3C. Aim: To investigate chemoprevention of high grade cervical neoplasia using Diindolylmethane (DIM) supplementation in women with low grade cytological abnormalities on cervical cytology. Objectives: To observe any reduction in the prevalence of histological proven high-grade cervical intraepithelial neoplasia (CIN) after 6 months of supplementation. ,,To observe any reduction in the prevalence of cytological abnormalities. ,,To observe any changes in the clinical appearance of the cervix. To assess acceptability and monitor any side effects of DIM supplementation. ,,To assess whether any benefit is seen in relation to Human Papillomavirus (HPV) status including HPV Type, Viral load and integration. Methods: This is a double blind randomized placebo-controlled trial involving 600,700 women with low grade cytological abnormalities on a cervical smear. Randomization is in the ratio of 2 : 1 in favour of active medication. Women with first mildly dyskaryotic smear or second borderline smear are eligible. They are asked to take two capsules daily for 6 months. At the end of 6 months they undergo repeat cervical cytology, HPV testing and colposcopy. Results: A progress report will be given for this ongoing study. [Funded: - Cancer Research UK] Type Specific HPV Infection in Welsh Cervical Cancers Background: Whilst there have been numerous studies of HPV infection associated with cervical cancer and on prevalence of Human Papillomavirus in diverse populations there have been no studies of these variables in the same population. Against a background of prophylactic HPV vaccination it is important to assess potential protection against cervical cancer within a given population. The most comprehensive analysis of HPV type specific cervical cancer is a meta-analysis published by the IARC in 2003. This however included only three UK based studies, totalling 118 cases, 75 of which were only investigated by HPV type PCR for four high risk types. None of this data was presented with associated population based prevalence data. Therefore, the research objectives for this study in combination with the first study above, are as follows: To determine the frequency of specific HPV types in cervical cancers in Wales. To compare the distribution of specific HPV types amongst cervical cancers with their prevalence in the general population. This will allow accurate delineation of the relationship between prevalence of specific HPV types in the general population and their association with clinically relevant disease. This information is a pre-requisite to assess the potential impact of prophylactic vaccination against HPV infection in Wales. Methods: Welsh Cervical Cancer specimens from 2000,2005 will be identified from pathology departments within Wales. The pathology of each tumour will be reviewed by a single Gynaecological Pathologist. The age of the patient and pathological features of the tumour will be noted. DNA will be extracted from the paraffin sections and HPV typed by PCR-EIA. Results: A progress report will be given for this ongoing study. [Funded by Welsh Office for Research and Development] [source] Hysteretic energy spectrum and damage controlEARTHQUAKE ENGINEERING AND STRUCTURAL DYNAMICS, Issue 12 2001Rafael Riddell Abstract The inelastic response of single-degree-of-freedom (SDOF) systems subjected to earthquake motions is studied and a method to derive hysteretic energy dissipation spectra is proposed. The amount of energy dissipated through inelastic deformation combined with other response parameters allow the estimation of the required deformation capacity to avoid collapse for a given design earthquake. In the first part of the study, a detailed analysis of correlation between energy and ground motion intensity indices is carried out to identify the indices to be used as scaling parameters and base line of the energy dissipation spectrum. The response of elastoplastic, bilinear, and stiffness degrading systems with 5 per cent damping, subjected to a world-wide ensemble of 52 earthquake records is considered. The statistical analysis of the response data provides the factors for constructing the energy dissipation spectrum as well as the Newmark,Hall inelastic spectra. The combination of these spectra allows the estimation of the ultimate deformation capacity required to survive the design earthquake, capacity that can also be presented in spectral form as an example shows. Copyright © 2001 John Wiley & Sons, Ltd. [source] System zones in capillary zone electrophoresis: Moving boundaries caused by freely migrating hydrogen ionsELECTROPHORESIS, Issue 2 2005Jozef L. Beckers Abstract We demonstrate that system zones (SZs) can be expected in background electrolytes (BGEs) with a low buffer capacity. The mobilities of this type of SZ (mSZ) could be determined by calculations both based on a mathematical model and by a simulation program. The values of mSZ are increasing for decreasing buffer capacities and lower concentrations of the BGEs. For completely unbuffered BGEs with a pH below 7, the mSZ reached values up to 350×10,9 m2V,1s,1. This value indicates that the existence of this type of SZ originates from migrating hydrogen ions. Although both the mathematical model and simulation program do not consider the influence of the pH of the sample solution, experiments have shown that the pH of the sample solution is also very important. The lower the pH of the sample solution, the larger the mSZ in a specific BGE for cationic SZs. Using completely unbuffered BGEs the hydrogen ions present in the original sample start to migrate freely through the capillary tube with the mobility of a single hydrogen ion and cause stepwise disturbances in the base line of the detector trace. It is remarkable that this type of SZ appears not to affect the electromigration dispersion (EMD) in a strong way and so far only dips could be obtained experimentally. [source] Evaluation of glargine group-start sessions in patients with type 2 diabetes as a strategy to deliver the serviceINTERNATIONAL JOURNAL OF CLINICAL PRACTICE, Issue 2 2007A. A. Tahrani Summary Improving glycaemic control in patients with type 2 diabetes reduces microvascular complications. The national service framework for diabetes and the new general medical service contract have been aiming to direct more focus on improving HbA1c. These measures have resulted in increasing number of patients being initiated on insulin therapy, which increases the workload of diabetes specialist nurses (DSNs). Initiating insulin on a one-to-one basis is time consuming. As a result DSN-led insulin group-start sessions were introduced. To evaluate DSN-led glargine group-start and self-titration as a strategy of providing service. We assessed the impact of this method on the use of DSNs time, HbA1c and on patients' satisfaction. A prospective audit in a district general hospital. Groups of 5,7 patients received two 2-h sessions at weeks 0 and 2. During these sessions, patients were initiated on insulin glargine and received an educational package and a self-titration protocol. DSNs did not see patients after week 2. Patients were able to phone the DSNs for advice till the end of the titration period. Patients completed Diabetes Treatment Satisfaction Questionnaire (DTSQ) at baseline, week 2 and 12 months. Weight and HbA1c were assessed at base line and 12 months later. Twenty-nine consecutive patients were included. Baseline HbA1c improved at 6 months and remained stable at 12 months (medians 10.0, 8.7 and 8.9 respectively, p < 0.001). DTSQ score improved between week 0 and 2 and this was maintained at 12 months (medians 26, 35 and 34 respectively, p < 0.001). After week 2, the DSNs spent a median of 21 min advising patients by phone during the titration period. Weight did not increase significantly. In our centre, DSN-led insulin group-start sessions and self-titration improved glycaemic control. Patients were satisfied with this method of starting insulin. This was achieved with minimal DSNs time and input and proved to be effective, yet less time consuming. [source] Innovation and operation with robotized underwater systemsJOURNAL OF FIELD ROBOTICS (FORMERLY JOURNAL OF ROBOTIC SYSTEMS), Issue 6 2007Vincent Rigaud This paper reports on the status and design of the operational remotely operated vehicle "Victor 6000", rated for 6000 m depth and of the 3000 m depth autonomous underwater vehicle "Asterxx" operated by Ifremer the French Institute for Sea Exploitation. Victor 6000 is part of a global system built of subsystems, "scientific modules," winch and cable, dead weight and umbilical, positioning subsystem using acoustic ultra short base line (USBL) techniques, and software for data and dive management. In 2005 the system was equipped with a second toolsled dedicated to high resolution mapping of the sea bed, with both acoustical and optical devices. Asterxx, the first autonomous underwater vehicle (AUV) developed by IFREMER, is 4.5 m in length with a diameter of 0.69 m. Depending on the payload, its weight is between 600 and 800 kg in air,with a diving depth of 3000 m. Its cruising speed is between 0.5 and 2.5 ms. The AUV is capable of carrying various payloads for a wide spectrum of applications. The vehicle can cruise up to 100 km. For coastal applications this vehicle is operable by a limited crew potentially from a nonspecialized vessel. Both vehicles are used for oceanography as well as to continually evolve underwater systems to address new scientific challenges. © 2007 Wiley Periodicals, Inc. [source] Palatal plate of different designs for the suppression of masseter muscle activity during sleep.JOURNAL OF ORAL REHABILITATION, Issue 9 2002A challenge to the concept of placebo splint Several designs of palatal appliance have been reported for the treatment of craniomandibular disorders. However, the effect of these appliances has not yet been fully clarified. This study aimed to reveal the effect of palatal appliances of different design on masseter muscle activity during sleep. Four volunteers (three women and one man, mean age 36·0 years) were used in this study. For each subject, four different types of palatal appliances were used. The palatal appliances were (1) horse shoe type (1 mm thick), (2) thin appliance which covered the whole palate (1 mm thick), (3) thick appliance which filled the palatal concavity and (4) appliance with half the thickness of the third appliance. These appliances were worn for 1 week in a randomized sequence with 1-week interval between each appliance. Right masseter muscle EMG was recorded three nights per week at home. The EMG signal longer than 0·25 s was classified as a burst and a signal shorter than 0·25 s was classified as a brief burst of fragmentary myoclonus (FM). Mean number of burst per hour for four subjects were 7·99 ± 7·32, 3·52 ± 1·95, 5·90 ± 3·99, 2·87 ± 2·87 and 9·96 ± 6·02 for base line, appliance 1, 2, 3 and 4, respectively. Mean number of FM per hour were 136·86 ± 69·88, 90·53 ± 41·19, 99·49 ± 39·29, 71·66 ± 24·66 and 144·39 ± 42·97, respectively. From these results, it was suggested that the thick palatal appliance suppressed the masseter muscle activity during sleep most effectively. Also, the results for appliance 4 questioned the concept of placebo effect for non-occlusal appliances. [source] Estimation of the base line imagined as a muscular background for myoglobin determination by reflectance spectrometryANIMAL SCIENCE JOURNAL, Issue 6 2004Masatoshi IZUMIMOTO ABSTRACT Liquid extraction method for determination of myoglobin in muscle is not suitable because of changes in the derivatives and time required. Optical reflectance method is useful as a non-destructive and real-time measurement. However, the base line of reflectance method, the muscular reflectance background (MRB) imagined as colorless structural elements, has been unknown because of non-existence. The spectrum of the base line was analyzed as the analogy of bleached meat. A formula was derived for reflectance that had a linear relationship to the reciprocal of wavelength to the power 4. The reflectance characteristic of the MRB was not constant and the curve tended to rise with increasing wavelength. The reflectance at 700 nm was estimated to be 50%. By the characteristic analysis on Commission Internationale l'Eclairage color values and reciprocal reflectance, it was concluded that the reflectance spectrum of the muscular background obtained in this research was fitted as a base line for the reflectance spectrometry. [source] A comparison of different models of stroke on behaviour and brain morphologyEUROPEAN JOURNAL OF NEUROSCIENCE, Issue 7 2003C.L.R. Gonzalez Abstract We compared the effects of three models of permanent ischemia, as well as cortical aspiration, on behaviour and brain morphology. Rats received a stroke either by devascularization or by two different procedures of medial cerebral artery occlusion (MCAO; small vs. large). Animals were trained in a reaching task, forepaw asymmetry, forepaw inhibition, sunflower seed task and tongue extension. Behaviour was assessed 1 week after the lesion and at 2-week intervals for a total of 9 weeks. One week after the surgery all animals were severely impaired on all tasks and although they improved over time they only reached preoperative base lines on tongue extension. Animals with small MCAOs performed better in reaching and sunflower tasks; no other behavioural differences were detected among the groups. Pyramidal cells in forelimb and cingulate areas as well as spiny neurons of the striatum were examined for dendritic branching and spine density using a Golgi,Cox procedure. Each lesion type had a different impact on cell morphology. Overall, different changes (atrophy or hypertrophy) were observed with each kind of lesion and these changes were specific for the region (forelimb, cingulate, striatum) and the condition (intact vs. damaged hemisphere). These results suggest that: (i) different lesions to the motor cortex produce subtle differences in behaviour, and (ii) the method used to induce the lesion produces striking differences in cortical and subcortical plasticity. [source] | ||||||||||||||||