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Tenth Day (tenth + day)
Selected AbstractsScreening for novel ENU-induced rhythm, entrainment and activity mutantsGENES, BRAIN AND BEHAVIOR, Issue 4 2004Y. Bacon Chemical mutagenesis has provided an opportunity to develop and expand the repertoire of behavioural mutants for gene function studies. With this in mind, we have established a screen in mice for mutations affecting circadian rhythms, entrainment to light and other wheel-running parameters. The screen consists of an assessment of mouse wheel-running activity in a 12:12 h light/dark cycle for 7,10 days followed by assessment in constant darkness for up to 20 days. Responses to light are assessed using two protocols; a 15 minute light pulse given at circadian time 16 on the tenth day in constant darkness and an additional 12 h of light upon transition from light/dark conditions to constant darkness. To date, approximately 1300 progeny of chemically mutagenised mice have been screened. Computer-aided assessment of wheel-running parameters has helped in identifying abnormal phenotypes in approximately 5% of all animals screened. Inheritance testing of mice with abnormal phenotypes has confirmed the number of robustly inherited mutant phenotypes to be 1% of the total screened. Confirmed mutants including those affecting free-running period, light-responsiveness and wheel-running endurance have been identified. Thus far, low-resolution map positions have been established for four mutants by completing genome scans in backcross progeny. Mutant loci do not correspond with those previously associated with wheel-running behaviour. This result confirms that phenotype-driven approaches such as this should continue to provide material for mammalian gene function studies. [source] Alveolar capillary dysplasia with antenatal anomalies mimicking trisomy 21JOURNAL OF PAEDIATRICS AND CHILD HEALTH, Issue 1 2001J. McGaughran Abstract: Alveolar capillary dysplasia (ACD) has been described in conjunction with a number of congenital abnormalities. The case reported here was noted in utero to have duodenal atresia and a partial atrioventricular canal defect and a provisional diagnosis of trisomy 21 was considered. A fetal blood sample showed a normal karyotype. The diagnosis of ACD was made at post-mortem following a neonatal death on the tenth day. This case further highlights the range of congenital abnormalities that may be present in cases of ACD that may mimic other conditions, including trisomy 21, on antenatal scan. However, the absence of congenital anomalies, even in the same family, would not exclude the diagnosis of ACD. [source] Changes in Cell Wall-bound Phenolic Compounds and Lignin in Roots of Date Palm Cultivars Differing in Susceptibility to Fusarium oxysporum f. sp. albedinisJOURNAL OF PHYTOPATHOLOGY, Issue 7-8 2000C. El Modafar The roots of date palm contain four cell wall-bound phenolic acids identified as p -hydroxybenzoic, p -coumaric, ferulic and sinapic acids. The ferulic acid represents the major phenolic compound since it constitutes 48.2,55.8% of cell wall-bound phenolic acids. All these phenolic acids were present in the resistant cultivar (BSTN) and the susceptible cultivar (JHL). However, the pre-infection contents of p -coumaric, ferulic and sinapic acids were greater in the resistant cultivar than in the susceptible one. For the contents of p -hydroxybenzoic acid, there was no significant difference between the resistant cultivar and the susceptible cultivar. Similarly, the pre-infection contents of lignin were approximately equal for both cultivars. Inoculation of the date palm roots by Fusarium oxysporum f. sp. albedinis induced important modifications to the contents of the cell wall-bound phenolic compounds and lignin, which made it possible to distinguish between resistant and susceptible cultivars. The post-infection contents of cell wall-bound phenolic compounds underwent a rapid and intense increase with a maximum accumulation on the tenth day for p -hydroxybenzoic acid (1.54 ,mol/g), p -coumaric acid (2.77 ,mol/g) and ferulic acid (2.64 ,mol/g) and on the fifteenth day for sinapic acid (1.85 ,mol/g). The maximum contents accumulated in the resistant cultivar were greater than those in the susceptible cultivar, namely, 11 times for p -hydroxybenzoic acid, 2.6 times for p -coumaric acid, 1.8 times for ferulic acid and 12.3 times for sinapic acid. In the susceptible cultivar, p -coumaric acid and ferulic acid contents also increased after inoculation although they did not reach the pre-infection contents of the resistant cultivar. The contents of p -hydroxybenzoic acid in the susceptible cultivar roots did not present post-infection modification and those of sinapic acid decreased instead. The lignin contents increased in both cultivars with a maximum accumulation on the fifteenth day. However, the maximum contents accumulated in the resistant cultivar roots were 1.5 times greater than those of the susceptible cultivar. These results showed clear differences between the resistant BSTN and the susceptible JHL cultivars. The implication of cell wall-bound phenolic compounds and lignin in the resistance of date palm to F. oxysporum f. sp. albedinis appears to be dependent on the speed and intensity of their accumulation with greater contents in the first stage of infection. Zusammenfassung Die Wurzeln der Dattelpalme enthalten vier zellwandgebundene Phenolsäuren, die als p -Hydroxybenzoesäure, p- Cumarsäure, Ferulasäure und Sinapinsäure identifiziert wurden. Ferulasäure ist die wichtigste phenolische Verbindung, denn sie stellt 48,2 bi 55,8% der zellwandgebundenen Phenolsäuren. Alle vier Phenolsäuren waren in der resistenten Sorte BSTN und in der anfälligen Sorte JHL vorhanden. Die Gehalte an p -Cumarsäure, Ferulasäure und Sinapinsäure waren vor der Infektion bei der resistenten Sorte jedoch höher als bei der anfälligen Sorte. Hinsichtlich des Gehalts an p -Hydroxybenzoesäure unterschieden sich die resistente und die anfällige Sorte nicht signifikant voneinander. Auch die Ligningehalte vor der Infektion waren bei beiden Sorten ungefähr gleich. Die Inokulation von Dattelpalmenwurzeln mit Fusarium oxysporum f. sp. albedinis induziert wichtige Änderungen der Gehalte an zellwandgebundenen phenolischen Verbindungen und Lignin, was eine Unterscheidung resistenter von anfälligen Sorten ermöglicht. Nach der Infektion nehmen die Gehalte an zellwandgebundenen phenolischen Verbindungen rasch und erheblich zu, mit maximaler Akkumulation am 10. Tag bei p -Hydroxybenzoesäure (1,54 ,mol/g), p -Cumarsäure (2,77 ,mol/g) und Ferulasäure (2,64 ,mol/g) sowie am 15. Tag bei Sinapinsäure (1,85 ,mol/g). Die in der resistenten Sorte akkumulierten maximalen Gehalte waren höher als die maximalen Gehalte der anfälligen Sorte , um das Elffache bei p -Hydroxybenzoesäure, das 2,6-fache bei p -Cumarsäure, das 1,8-fache bei Ferulasäure und das 2,3-fache bei Sinapinsäure. Bei der anfälligen Sorte steigen die Gehalte an p -Cumarsäure und Ferulasäure nach der Inokulation ebenfalls an, bleiben aber niedriger als die Werte der resistenten Sorte vor der Infektion. Der p -Hydroxybenzoesäuregehalt in den Wurzeln der anfälligen Sorte ist nach der Infektion nicht verändert, und der Sinapinsäuregehalt nimmt ab. Der Ligningehalt steigt bei beiden Sorten, mit maximaler Akkumulation am 15. Tag. Der maximale Gehalt in den Wurzeln der resistenten Sorte war jedoch 1,5-mal höher als bei der anfälligen Sorte. Diese Ergebnisse zeigen deutliche Unterschiede zwischen der resistenten Sorte (BSTN) und der anfälligen Sorte (JHL). Die Bedeutung der zellwandgebundenen phenolischen Verbindungen und des Lignins für die Resistenz der Dattelpalme gegen F. oxysporum f. sp. albedinis scheint von der Geschwindigkeit und der Intensität der Akkumulation abzuhängen, wobei die Gehalte im ersten Stadium der Infektion höher sind. [source] Severe Legionella pneumonia successfully treated by independent lung ventilation with intrapulmonary percussive ventilationRESPIROLOGY, Issue 3 2008Motoki FUJITA Abstract: A case of severe Legionella pneumonia was successfully treated by independent lung ventilation (ILV) with intrapulmonary percussive ventilation (IPV). A 57-year-old man with lobar pneumonia was intubated and mechanically ventilated because of his deteriorating respiratory status. The diagnosis of Legionella pneumonia was made on the fourth day after admission and appropriate antibiotic therapy was commenced. On the fifth hospital day, ILV was commenced because the right unaffected lung was over-distended, his haemodynamic state was unstable and his left lung was producing copious amounts of purulent sputum. His right lung was ventilated and his left lung was treated with IPV owing to the existence of massive atelectasis. After treatment with antibiotics and ILV combined with IPV, his respiratory and haemodynamic status gradually improved. On the tenth day after admission, ILV was changed to conventional bilateral ventilation. The patient was extubated on the sixteenth hospital day and discharged from the intensive care unit 30 days after admission. The combination of ILV and IPV was therapeutically effective during the acute phase of unilateral severe Legionella pneumonia. [source] | |||||||||||||