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Swim Bladder (swim + bladder)

Distribution by Scientific Domains

Earth and Environmental Science	57%
Life Sciences	42%

Selected Abstracts

Isolation of a Carnobacterium maltaromaticum- like bacterium from systemically infected lake whitefish (Coregonus clupeaformis)

FEMS MICROBIOLOGY LETTERS, Issue 1 2008
Thomas P. Loch
Abstract Herein we report on the first isolation of a Carnobacterium maltaromaticum -like bacterium from kidneys and swim bladders of lake whitefish (Coregonus clupeaformis) caught from Lakes Michigan and Huron, Michigan. Isolates were Gram-positive, nonmotile, facultatively anaerobic, asporogenous rods that did not produce catalase, cytochrome oxidase, or H2S, and did not grow on acetate agar. Except for carbohydrate fermentation, many phenotypic characteristics of lake whitefish isolates coincided with those of C. maltaromaticum, the causative agent of pseudokidney disease. Partial sequencing of 16S and 23S rRNA genes, as well as the piscicolin 126 precursor gene, yielded 97% and 98% nucleotide matches with C. maltaromaticum, respectively (accession numbers EU546836 and EU546837; EU643471). Phylogenetic analyses showed that lake whitefish isolates of this study are highly related, yet not fully identical to C. maltaromaticum. The presence of the C. maltaromaticum -like bacterium was associated with splenomegaly, renal and splenic congestion, and thickening of the swim bladder wall with accumulation of a mucoid exudate. Examination of stained tissue sections revealed renal and splenic congestion, vacuolation and bile stasis within the liver, and hyperplasia within the epithelial lining of the swim bladder. The concurrent presence of pathological changes and the C. maltaromaticum -like bacteria suggests that this bacterium is pathogenic to lake whitefish. [source]

The sampling efficiency of electrofishing for Neogobius species in a riprap habitat: a field experiment

JOURNAL OF APPLIED ICHTHYOLOGY, Issue 5 2008
M. Pola
Summary Even though electrofishing is commonly used to sample Neogobius spp. and other swimbladder-lacking benthic fishes, its efficiency is considered poor especially in habitats with abundant interstitial spaces. To determine the efficiency of electrofishing Neogobius spp. and quantitatively estimate sampling bias in a riprap (shot rock used to armor shorelines against water erosion) mesohabitat, riprap fragments were set up in a natural riverine environment. The experimental setting enabled us to collect all fish remaining in the riprap fragments after these areas had been electrofished. The sampling efficiency of electrofishing Neogobius spp. (dominated by Neogobius melanostomus) varied between 17.6 and 47.4% (mean 29.7%), while percids (possessing a well-developed swim bladder) were collected with 74.6% efficiency. Fish size had no effect on the probability of capturing Neogobius spp. by electrofishing. Within Neogobius spp., N. melanostomus was less susceptible to electrofishing than Neogobius gymnotrachelus (23.7% and 50.1%, respectively). Decreased electrofishing efficiency in areas of rocky substrate should be considered in estimates of total abundance of Neogobius spp., especially if they are to be compared with catches of other species possessing swim bladders. [source]

Production of Isin glass from the swim bladder of sturgeons

JOURNAL OF APPLIED ICHTHYOLOGY, Issue 2006
Anoosheh Koochekian
No abstract is available for this article. [source]

Caspase-dependent induction of apoptosis in barramundi, Lates calcarifer (Bloch), muscle cells by grouper iridovirus

JOURNAL OF FISH DISEASES, Issue 12 2009
P P Chiou
Abstract We recently reported that grouper iridovirus (GIV) can induce apoptosis in barramundi, Lates calcarifer, muscle (BM) and swim bladder (BSB) cell lines. In this paper, we further characterize the molecular mechanism underlying apoptotic death in BM cells triggered by GIV. DNA-laddering and apoptotic cells were observed in BM cells infected with UV-irradiated or untreated GIV but was absent in cells infected with heat-inactivated GIV, indicating the involvement of viral protein in the apoptosis event. In GIV-infected BM cells, the conversion of procaspase-3 to caspase-3 was evident and the level of caspase-8 and -9 increased as early as 30 min post-infection. When treated with a pancaspase inhibitor, the GIV-induced apoptosis event was abolished. These observations indicate that GIV-induced apoptosis is caspase-dependent, and that both the external and internal routes in the caspase-dependent pathway are likely involved in the apoptosis process. [source]

Development and characterization of three new diploid cell lines from Labeo rohita (Ham.)

BIOTECHNOLOGY PROGRESS, Issue 4 2010
Wazir S. Lakra
Abstract Development of cell lines from fish for identifying the pathogenesis of viral diseases and for vaccine production against viral and bacterial diseases is imperative where they are of commercial importance. Three new diploid fish cell lines (RF, RH, and RSB) were developed from fin, heart, and swim bladder of an Indian major carp, Labeo rohita, commonly called Rohu. All the cell lines were optimally maintained at 28°C in Leibovitz-15 medium supplemented with 10% FBS. The propagation of RH and RSB cells was serum dependent, with a low plating efficiency (<16%), whereas RF cells showed 20% efficiency. The cytogenetic analysis revealed a diploid count of 50 chromosomes. The cells of RF and RSB were found to be epithelial, where as the cells of RH were mostly fibroblastic. The viability of the RF, RH, and RSB cell lines was 75, 70 and 72%, respectively after 6 months of storage in liquid nitrogen. The origin of the cell lines was confirmed by the amplification of 496 and 655 bp fragments of 16S rRNA and Cytochrome Oxidase Subunit I (COI) of mtDNA. The new cell lines would facilitate viral disease diagnosis and genomic studies. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010 [source]