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Surrounding Medium (surrounding + medium)
Selected AbstractsInability of Escherichia coli to resuscitate from the viable but nonculturable stateFEMS MICROBIOLOGY ECOLOGY, Issue 1 2007Inés Arana Abstract After induction of the viable but nonculturable (VBNC) state in Escherichia coli populations, we analysed abiotic and biotic factors suggested to promote the resuscitation process. The response to the stressing conditions implied the formation of three subpopulations, culturable, VBNC and nonviable. In most adverse situations studied, the VBNC subpopulation did not represent the dominant fraction, decreasing with time. This suggests that, in most cases, the VBNC is not a successful phenotype. Combining methods of dilution and inhibition of remaining culturable cells, we designed a working protocol in order to distinguish unequivocally between regrowth and resuscitation. Reversion of abiotic factors inducing nonculturability as well as prevention of additional oxidative stress did not provoke resuscitation. Participation of biotic factors was studied by addition of supernatants from different origin without positive results. These results indicate that the E. coli strain used is not able to resuscitate from the VBNC state. VBNC cells release into the surrounding medium, and could thus aid in the survival of persisting culturable cells. The formation of a VBNC subpopulation could thus be considered as an adaptive process, designed for the benefit of the population as a whole. [source] Cover Picture: Biomineralized Polysaccharide Capsules for Encapsulation, Organization, and Delivery of Human Cell Types and Growth Factors (Adv. Funct.ADVANCED FUNCTIONAL MATERIALS, Issue 6 2005Mater. Abstract The cover shows biomineralized polysaccharide capsules with specifiable make-up, which can provide microenvironments for stabilization, growth, and differentiation of human cell types, as reported by Oreffo and co-workers on p.,917. The capsules are amenable to complexation with a range of bioactive molecules and cells, offering tremendous potential as multifunctional scaffolds and delivery vehicles in tissue regeneration of hard and soft tissues. The construction of biomimetic microenvironments with specific chemical and physical cues for the organization and modulation of a variety of cell populations is of key importance in tissue engineering. We show that a range of human cell types, including promyoblasts, chondrocytes, adipocytes, adenovirally transduced osteoprogenitors, immunoselected mesenchymal stem cells, and the osteogenic factor, rhBMP-2 (BMP: bone morphogenic protein), can be successfully encapsulated within mineralized polysaccharide capsules without loss of function in vivo. By controlling the extent of mineralization within the alginate/chitosan shell membrane, degradation of the shell wall and release of cells or rhBMP-2 into the surrounding medium can be regulated. In addition, we describe for the first time the ability to generate bead-in-bead capsules consisting of spatially separated cell populations and temporally separated biomolecule release, entrapped within alginate/chitosan shells of variable thickness, mineralization, and stability. Such materials offer significant potential as multifunctional scaffolds and delivery vehicles in tissue regeneration of hard and soft tissues. [source] Biomineralized Polysaccharide Capsules for Encapsulation, Organization, and Delivery of Human Cell Types and Growth Factors,ADVANCED FUNCTIONAL MATERIALS, Issue 6 2005W. Green Abstract The construction of biomimetic microenvironments with specific chemical and physical cues for the organization and modulation of a variety of cell populations is of key importance in tissue engineering. We show that a range of human cell types, including promyoblasts, chondrocytes, adipocytes, adenovirally transduced osteoprogenitors, immunoselected mesenchymal stem cells, and the osteogenic factor, rhBMP-2 (BMP: bone morphogenic protein), can be successfully encapsulated within mineralized polysaccharide capsules without loss of function in vivo. By controlling the extent of mineralization within the alginate/chitosan shell membrane, degradation of the shell wall and release of cells or rhBMP-2 into the surrounding medium can be regulated. In addition, we describe for the first time the ability to generate bead-in-bead capsules consisting of spatially separated cell populations and temporally separated biomolecule release, entrapped within alginate/chitosan shells of variable thickness, mineralization, and stability. Such materials offer significant potential as multifunctional scaffolds and delivery vehicles in tissue regeneration of hard and soft tissues. [source] Poroelastic model for pile,soil interaction in a half-space porous medium due to seismic wavesINTERNATIONAL JOURNAL FOR NUMERICAL AND ANALYTICAL METHODS IN GEOMECHANICS, Issue 1 2008Jian-Fei Lu Abstract In this paper, frequency domain dynamic response of a pile embedded in a half-space porous medium and subjected to P, SV seismic waves is investigated. According to the fictitious pile methodology, the problem is decomposed into an extended poroelastic half-space and a fictitious pile. The extended porous half-space is described by Biot's theory, while the fictitious pile is treated as a bar and a beam and described by the conventional 1-D structure vibration theory. Using the Hankel transformation method, the fundamental solutions for a half-space porous medium subjected to a vertical or a horizontal circular patch load are established. Based on the obtained fundamental solutions and free wave fields, the second kind of Fredholm integral equations describing the vertical and the horizontal interaction between the pile and the poroelastic half-space are established. Solution of the integral equations yields the dynamic response of the pile to plane P, SV waves. Numerical results show the parameters of the porous medium, the pile and incident waves have direct influences on the dynamic response of the pile,half-space system. Significant differences between conventional single-phase elastic model and the poroelastic model for the surrounding medium of the pile are found. Copyright © 2007 John Wiley & Sons, Ltd. [source] A unified approach for the formulation of interaction problems by the boundary element methodINTERNATIONAL JOURNAL FOR NUMERICAL METHODS IN ENGINEERING, Issue 5 2006Yalcín Mengi Abstract A unified formulation is presented, based on boundary element method, in a form suitable for performing the interaction analyses by substructure method for solid,solid and soil,structure problems. The proposed formulation permits the evaluation of all the elements of impedance and input motion matrices simultaneously at a single step in terms of system matrices of the boundary element method without solving any special problem, such as, unit displacement or load problem, as required in conventional methods. It eliminates further the complicated procedure and the need for using scattering analysis in the evaluation of input motion functions. To explain the formulation, it is first given for an inclusion interacting with an infinite surrounding medium under the influence of a seismic input, where both the inclusion and surrounding medium are treated as viscoelastic. It is shown that the formulation for a rigid inclusion may be obtained from that for flexible inclusion as a special case through a transformation. Then, the formulation is extended to other types of interaction problems: a multi-inclusion problem and an interaction problem involving a foundation embedded in a viscoelastic half-space. It is found that the proposed formulation remains essentially the same for all kinds of interaction problems and it can be used not only in regular interaction analysis, but also in the analysis involving diffraction of waves in a medium containing holes. Copyright © 2005 John Wiley & Sons, Ltd. [source] On optimization of bio-probesINTERNATIONAL JOURNAL FOR NUMERICAL METHODS IN ENGINEERING, Issue 6 2004N. L. Pedersen Abstract The present paper deals with the modelling and optimization of small bio-probes that can be used for biological sensing; the bio-probes can be classified as MicroElectroMechnical Systems (MEMS). The objective is to optimize the structure of the bio-probes in order to maximize the sensing sensitivity. A biological coating results in a prestress on the sensing cantilever when certain molecules are present in the surrounding medium. The mechanical deformation due to the biological material is modelled by applying a prestress in the top layer of the bio-probes. Topology optimization is used to improve the design. In the present work it is necessary to use an interpolation scheme different from the SIMP (power law) approach which is usually used in topology optimization. In calculating the sensitivities, needed for the optimization, complications due to the prestress occur, but also due to the coupling between the elastic field and the electric field which both must be used in an integrated model. These complications are dealt with and analytically obtained sensitivities are presented. Copyright © 2004 John Wiley & Sons, Ltd. [source] Ingredients Necessary for Proton Transfer in EnzymesISRAEL JOURNAL OF CHEMISTRY, Issue 2 2009Steve Scheiner The transfer of a proton across a hydrogen bond can be influenced by a number of factors, including H-bond length, intramolecular angles, and the presence of neighboring groups. The ability of different factors to push a proton across the H-bond is examined for a specific and very important pair of catalytic groups, Ser-195 and His-57, within the context of a serine proteinase enzyme. The influence of residue Asp-102 is considered for different charge states, as is the nature of the surrounding medium. Also examined are the perturbations introduced by the substrate and external ions and dipoles. [source] Application of small-angle scattering to study the effects of moisture content on a native soy proteinJOURNAL OF APPLIED CRYSTALLOGRAPHY, Issue 3 2008Catherine S. Kealley The nano- and microstructure of glycinin, a soybean protein, has been investigated as a function of moisture for moisture contents between 4 and 21,wt%. Glycinin exhibits peaks in the small-angle region whose positions show minimal change with X-rays for samples up to 13% moisture. However, the use of neutron scattering, and the associated enhancement in contrast, results in the Bragg peaks being well resolved up to higher moisture contents; the associated shift in peak positions between 4 and 21% moisture are consistent with the expansion of a hexagonal unit cell as a function of moisture content. A Porod slope of ,,4 indicates that the interface between the `dry' protein powder and the surrounding medium at a length-scale of at least 3,µm down to ,20,nm is smooth and sharp. Scanning electron microscopy indicates that the powders, with low moisture content, have a porous appearance, with the porosity decreasing and microstructure expanding as the moisture content increases. [source] The metabolic component of cellular refractivity and its importance for optical cytometryJOURNAL OF BIOPHOTONICS, Issue 8-9 2009V. TychinskyArticle first published online: 30 JUL 200 Abstract Initially, it has been shown that the phase thickness and refractivity (the latter interpreted as the difference of the refractivity indices of an object and surrounding medium) depend on the functional state of mitochondria. The refractivity of various objects decreased in response to energy depletion. This dependence was then demonstrated for other biological objects such as cyanobacteria, chloroplasts and human cells. This general response brought about the hypothesis of a certain "universal" factor that links the variable (or metabolic) component of refractivity with the object's functional state. However, the origin of this phenomenon remains unknown. Our hypothesis is founded on the dependence of polarization of bound water molecules and the activity of metabolic processes. Here, we show the results of measurements of metabolic component of refractivity different bio-objects (mitochondria, chloroplasts, spores, cancer cells) obtained using the Coherent Phase Microscope "Airyscan". Estimations indicated high (up to n , 1.41,1.45) values for the equivalent refractive index of structured water in cells. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source] Novel materials for bioanalytical and biomedical applications: Environmental response and binding/release capabilities of amphiphilic hydrogels with shape-persistent dendritic junctions,JOURNAL OF POLYMER SCIENCE (IN TWO SECTIONS), Issue 18 2005Chao Zhu Abstract The binding and release capabilities of a hydrogel series, constructed of hydrophilic poly(ethylene glycol) segments and hydrophobic dendritic junctions [poly (benzyl ether)s], are evaluated in aqueous media. The environmental response of the amphiphilic networks is also tested in water at three pH values: 1.5, 7.0, and 10.1. The highest swelling ratio is observed under acidic conditions and varies between 3.7 and 6.5, depending on the crosslinking density and dendrimer generation. Gel specimens with embedded indicators react within 3,6 s with a clear color switch to the change in the pH of the surrounding medium. The experiments with model anionic and cationic indicators and stains show that the hydrogels have basic interiors. The gel binding capabilities depend on the water solubility of the substrate and on the size of the incorporated dendritic fragments. Model release studies have been performed at 37 °C and pHs 1.5, 7.0, and 10.1. The observed phenomena are explained by the transformations in the structure and charge that both the networks and the model compounds undergo with the changes in the pH of the aqueous medium. © 2005 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 43: 4017,4029, 2005 [source] The intensity of the 1602 cm,1 band in human cells is related to mitochondrial activityJOURNAL OF RAMAN SPECTROSCOPY, Issue 5 2009Vishnu Vardhan Pully Abstract We report a Raman band at 1602 cm,1 in the spectra of human cells, which previously had only been observed in mitochondria of yeast cells. This band, which has not yet been assigned to a particular molecular species, was found to occur in HeLa cells, peripheral blood lymphocytes, human mesenchymal stem cells and bovine chondrocytes. The band is proposed as an indicator of the activity of mitochondria in cells. Cells were cultured with and without serum or temporarily deprived of serum. The band can be observed for all these variations in cell culture methodology. The band intensity decreases under the influence of an increase of the calcium ion concentration in the surrounding medium. Copyright © 2009 John Wiley & Sons, Ltd. [source] Structures of the magnetoionic media around the Fanaroff,Riley Class I radio galaxies 3C 31 and Hydra AMONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 2 2008R. A. Laing ABSTRACT We use high-quality Very Large Array (VLA) images of the Fanaroff,Riley Class I radio galaxy 3C 31 at six frequencies in the range 1365,8440 MHz to explore the spatial scale and origin of the rotation measure (RM) fluctuations on the line of sight to the radio source. We analyse the distribution of the degree of polarization to show that the large depolarization asymmetry between the north and south sides of the source seen in earlier work largely disappears as the resolution is increased. We show that the depolarization seen at low resolution results primarily from unresolved gradients in a Faraday screen in front of the synchrotron-emitting plasma. We establish that the residual degree of polarization in the short-wavelength limit should follow a Burn law and we fit such a law to our data to estimate the residual depolarization at high resolution. We discuss how to interpret the structure function of RM fluctuations in the presence of a finite observing beam and how to address the effects of incomplete sampling of RM distribution using a Monte Carlo approach. We infer that the observed RM variations over selected areas of 3C 31, and the small residual depolarization found at high resolution, are consistent with a power spectrum of magnetic fluctuations in front of 3C 31 whose power-law slope changes significantly on the scales sampled by our data. The power spectrum P(f) can only have the form expected for Kolmogorov turbulence [P(f) ,f,11/3] on scales ,5 kpc. On larger scales, we find . We briefly discuss the physical interpretation of these results. We also compare the global variations of RM across 3C 31 with the results of three-dimensional simulations of the magnetic-field fluctuations in the surrounding magnetoionic medium. We infer that the RM variation across 3C 31 is qualitatively as expected from relativistic-jet models of the brightness asymmetry wherein the apparently brighter jet is on the near side of the nucleus and is seen through less magnetoionic material than the fainter jet. We show that our data are inconsistent with observing 3C 31 through a spherically symmetric magnetoionic medium, but that they are consistent with a field distribution that favours the plane perpendicular to the jet axis , probably because the radio source has evacuated a large cavity in the surrounding medium. We also apply our analysis techniques to the case of Hydra A, where the shape and the size of the cavities produced by the source in the surrounding medium are known from X-ray data. We emphasize that it is essential to account for the potential exclusion of magnetoionic material from a large volume containing the radio source when using the RM variations to derive statistical properties of the fluctuations in the foreground magnetic field. [source] On the evolution of young radio-loud AGNMONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 2 2000I. A. G. Snellen This paper describes an investigation of the early evolution of extragalactic radio sources using samples of faint and bright gigahertz peaked spectrum (GPS) and compact steep spectrum (CSS) radio galaxies. Correlations found between their peak frequency, peak flux density and angular size provide strong evidence that synchrotron self-absorption is the cause of the spectral turnovers, and indicate that young radio sources evolve in a self-similar way. In addition, the data seem to suggest that the sources are in equipartition while they evolve. If GPS sources evolve to large size radio sources, their redshift dependent birth-functions should be the same. Therefore, since the lifetimes of radio sources are thought to be short compared to the Hubble time, the observed difference in redshift distribution between GPS and large size sources must be due to a difference in slope of their luminosity functions. We argue that this slope is strongly affected by the luminosity evolution of the individual sources. A scenario for the luminosity evolution is proposed in which GPS sources increase in luminosity and large-scale radio sources decrease in luminosity with time. This evolution scenario is expected for a ram-pressure confined radio source in a surrounding medium with a King profile density. In the inner parts of the King profile, the density of the medium is constant and the radio source builds up its luminosity, but after it grows large enough the density of the surrounding medium declines and the luminosity of the radio source decreases. A comparison of the local luminosity function (LLF) of GPS galaxies with that of extended sources is a good test for this evolution scenario. Unfortunately, only a handful of GPS sources are known at low redshift, and an LLF can only be derived, assuming that their cosmological number density evolution is similar to that of steep spectrum sources. The LLF derived in this way is shown to be in good agreement with the proposed evolution scenario. However, the uncertainties are large, and larger, homogeneously selected samples of GPS sources are needed. [source] Fractional contribution of major ions to the membrane potential of Drosophila melanogaster oocytesARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY (ELECTRONIC), Issue 4 2009Susan M. Munley Abstract In ovarian follicles of Drosophila melanogaster, ion substitution experiments revealed that K+ is the greatest contributor (68%) in setting oocyte steady-state potential (Em), while Mg2+ and a metabolic component account for the rest. Because of the intense use made of Drosophila ovarian follicles in many lines of research, it is important to know how changes in the surrounding medium, particularly in major diffusible ions, may affect the physiology of the cells. The contributions made to the Drosophila oocyte membrane potential (Em) by [Na+]o, [K+]o, [Mg2+]o, [Ca2+]o, [Cl,]o, and pH (protons) were determined by substitutions made to the composition of the incubation medium. Only K+ and Mg2+ were found to participate in setting the level of Em. In follicles subjected to changes in external pH from the normal 7.3 to either pH 6 or pH 8, Em changed rapidly by about 6,mV, but within 8,min had returned to the original Em. Approximately half of all follicles exposed to reduced [Cl,]o showed no change in Em, and these all had input resistances of 330,k, or greater. The remaining follicles had smaller input resistances, and these first depolarized by about 5,mV. Over several minutes, their input resistances increased and they repolarized to a value more electronegative than their value prior to reduction in [Cl,]o. Together, K+ and Mg2+ accounted for up to 87% of measured steady-state potential. Treatment with sodium azide, ammonium vanadate, or chilling revealed a metabolically driven component that could account for the remaining 13%. © 2009 Wiley Periodicals, Inc. [source] Gas and stars in compact (young) radio sourcesASTRONOMISCHE NACHRICHTEN, Issue 2-3 2009R. Morganti Abstract Gas can be used to trace the formation and evolution of galaxies as well as the impact that the nuclear activity has on the surrounding medium. For nearby compact radio sources, we have used observations of neutral hydrogen , that we detected in emission distributed over very large scales , combined with the study of the stellar population and deep optical images to investigate the history of the formation of their host galaxy and the triggering of the activity. For more distant and more powerful compact radio sources, we have used optical spectra and H I , in absorption , to investigate the presence of fast outflows that support the idea that compact radio sources are young radio loud AGN observed during the early stages of their evolution and currently shredding their natal cocoons through extreme circumnuclear outflows. We will review the most recent results obtained from these projects (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source] Mid-Infrared Imaging and Spectroscopic Observations of the Galactic Center with Subaru/COMICSASTRONOMISCHE NACHRICHTEN, Issue S1 2003Y. Okada Abstract We report the results of mid-infrared (7.8,m,13.2 ,m) high-spatial resolution imaging and spectroscopic observations of the Galactic center region with the Cooled Mid-Infrared Camera and Spectrometer (COMICS) on the Subaru telescope. The images clearly show bright infrared sources and small structures in the diffuse emission. The spectra of all the observed positions show the 9.7 ,m silicate absorption feature. After corrected for the empirically-derived extinction, the intrinsic spectra of the infrared sources show either strong silicate emission or absorption, while the intrinsic diffuse emission has a power-law type spectrum. This difference indicates a possibility of dust processing due to the interaction between the infrared sources and their surrounding medium or a different origin of the dust grains surrounding the sources from those in the diffuse region. [source] Effect of Polarity and Structural Design on Molecular Photorefractive Properties of Heteroaromatic-Based Push,Pull DyesCHEMISTRY - A EUROPEAN JOURNAL, Issue 27 2006Graziano Archetti Dr. Abstract A combined experimental (optical and electro-optical absorption measurements) and computational (ab initio RHF and DFT) approach has been used to investigate the molecular low- and high- Tg photorefractive (PR) performances of neutral and zwitterionic heteroaromatic dipolar chromophores in terms of structural and solvent-polarity effects. We have found that the nature of the building units (donor, acceptor, and spacer) and the polarity of the surrounding medium strongly affect all the relevant ground-state and nonlinear optical properties involved in the PR activity, that is, the dipole moment, the polarizability anisotropy, and first hyperpolarizability of the electronic ground-state. The variation of these properties is in turn transferred to molecular low- and high- Tg PR figures of merit. It is shown that PR molecular performance not only relies on a proper choice of structural components but varies by orders of magnitude as a function of the medium polarity, and this suggests that a combination of molecular design and host-matrix engineering is required for optimized performances of PR materials. [source] | |||||||||||||||||||