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Selected AbstractsHairy Root Culture in a Liquid-Dispersed Bioreactor: Characterization of Spatial HeterogeneityBIOTECHNOLOGY PROGRESS, Issue 3 2000Gary R. C. Williams A liquid-dispersed reactor equipped with a vertical mesh cylinder for inoculum support was developed for culture of Atropa belladonna hairy roots. The working volume of the culture vessel was 4.4 L with an aspect ratio of 1.7. Medium was dispersed as a spray onto the top of the root bed, and the roots grew radially outward from the central mesh cylinder to the vessel wall. Significant benefits in terms of liquid drainage and reduced interstitial liquid holdup were obtained using a vertical rather than horizontal support structure for the biomass and by operating the reactor with cocurrent air and liquid flow. With root growth, a pattern of spatial heterogeneity developed in the vessel. Higher local biomass densities, lower volumes of interstitial liquid, lower sugar concentrations, and higher root atropine contents were found in the upper sections of the root bed compared with the lower sections, suggesting a greater level of metabolic activity toward the top of the reactor. Although gas-liquid oxygen transfer to the spray droplets was very rapid, there was evidence of significant oxygen limitations in the reactor. Substantial volumes of non-free-draining interstitial liquid accumulated in the root bed. Roots near the bottom of the vessel trapped up to 3,4 times their own weight in liquid, thus eliminating the advantages of improved contact with the gas phase offered by liquid-dispersed culture systems. Local nutrient and product concentrations in the non-free-draining liquid were significantly different from those in the bulk medium, indicating poor liquid mixing within the root bed. Oxygen enrichment of the gas phase improved neither growth nor atropine production, highlighting the greater importance of liquid-solid compared with gas-liquid oxygen transfer resistance. The absence of mechanical or pneumatic agitation and the tendency of the root bed to accumulate liquid and impede drainage were identified as the major limitations to reactor performance. Improved reactor operating strategies and selection or development of root lines offering minimal resistance to liquid flow and low liquid retention characteristics are possible solutions to these problems. [source] The hidden record: Late Holocene landscapes and settlement archaeology in the Lower Ohio River ValleyGEOARCHAEOLOGY: AN INTERNATIONAL JOURNAL, Issue 2 2002C. Russell Stafford From extensive backhoe trenching and coring in the Great Miami,Ohio River confluence region, in conjunction with a large suite of radiocarbon ages, we have identified large tracts of late Holocene alluvium. Thick blankets of Historic alluvium (post-settlement) may also cover levees of the Ohio River. In conjunction with other studies in the Outer Bluegrass Region of the lower Ohio River valley, it is possible to document an association between floodplain Mollisols, Inceptisols, and Entisols and late Holocene-aged landforms. Levees appear to have stabilized between 1000 and 2000 yr B.P. Although buried soils are rare, archaeological materials were ubiquitous in late Holocene Landform Sediment Assemblages. Because a substantial volume of valley fill is late Holocene in age, Woodland sites of all types are frequently buried, and Fort Ancient sites may be buried by Historic alluvium. Therefore, if Woodland or later settlement studies in this region are based on surface evidence alone, they are likely to be biased. © 2002 John Wiley & Sons, Inc. [source] Olive oil mill waste water as a replacement for fresh water in the manufacture of fired clay bricksJOURNAL OF CHEMICAL TECHNOLOGY & BIOTECHNOLOGY, Issue 8 2006Houda Mekki Abstract This paper describes the introduction of olive mill wastewater (OMW) to replace fresh water normally used in clay brick manufacture. OMW is recognised as the major agro-food industry pollutant in the Mediterranean/North African olive-growing region. The research involved adding OMW to laboratory-produced clay bricks following the same making procedure used at a collaborating Tunisian brick factory. The samples containing OMW were found to be comparable in forming/extrusion performance to a control product that used fresh water. If introduced at the factory scale, this innovation would allow a substantial volume of OMW to be recycled, saving on the fresh water currently used in the brick-forming process. During the subsequent brick drying operation, most of the OMW (,98% water) would be released as vapour. Once in the kiln, the remaining solids in the bricks (calorific value 21,23 MJ kg,1) would liberate additional heat, reducing the gross energy from fossil fuel currently required during firing. Copyright © 2006 Society of Chemical Industry [source] The effect of target lithology on the products of impact meltingMETEORITICS & PLANETARY SCIENCE, Issue 12 2008G. R. OSINSKI Impact events generate pressures and temperatures that can melt a substantial volume of the target; however, there remains considerable discussion as to the effect of target lithology on the generation of impact melts. Early studies showed that for impacts into crystalline targets, coherent impact melt rocks or "sheets" are formed with these rocks often displaying classic igneous structures (e.g., columnar jointing) and textures. For impact structures containing some amount of sedimentary rocks in the target sequence, a wide range of impact-generated lithologies have been described, although it has generally been suggested that impact melt is either lacking or is volumetrically minor. This is surprising given theoretical constraints, which show that as much melt should be produced during impacts into sedimentary targets. The question then arises: where has all the melt gone? The goal of this synthesis is to explore the effect of target lithology on the products of impact melting. A comparative study of the similarly sized Haughton, Mistastin, and Ries impact structures, suggests that the fundamental processes of impact melting are basically the same in sedimentary and crystalline targets, regardless of target properties. Furthermore, using advanced microbeam analytical techniques, it is apparent that, for the structures under consideration here, a large proportion of the melt is retained within the crater (as crater-fill impactites) for impacts into sedimentary-bearing target rocks. Thus, it is suggested that the basic products are genetically equivalent but they just appear different. That is, it is the textural, chemical and physical properties of the products that vary. [source] Making sense of emergency surgery in New South Wales: a position statementANZ JOURNAL OF SURGERY, Issue 3 2010Stephen A. Deane Abstract Background:, Emergency surgery is a major component of the provision of surgical services and makes up a substantial volume of the workload of surgeons in many hospitals. It is often more complex and surgically challenging than elective surgery. However, little attention has been concentrated on the management or resource requirements of emergency surgery. Method:, This article identifies principles for models of emergency surgery care and describes how they can be incorporated into a redesign of emergency surgery. They have been developed and are endorsed by experienced surgical staff routinely coping with the challenges of emergency surgery. Results:, The benefits of redesigning emergency surgery will be realized by an active partnership between managers, surgeons and surgical teams. The anticipated clinical benefits include improved patient outcomes, enhanced patient and surgical team satisfaction, and increased trainee supervision in emergency surgery. Significant management benefits will ensue from high rates of emergency operating theatre utilization, reduced patient cancellations and reduction in after-hours costs. This unplanned but predictable workload will be managed in a planned and predictable fashion. Conclusion:, Reform of emergency surgery services is a necessity and not a choice. The development of the emergency surgery guidelines for New South Wales is a step in the right direction. The principles identified in the guidelines should be adapted and implemented across Australia if sustainable, safe and efficient emergency surgery services are to be provided. Patients will expect nothing less. [source] Dissecting galaxies with quantitative spectroscopy of the brightest stars in the UniverseASTRONOMISCHE NACHRICHTEN, Issue 5 2010R.-P. KudritzkiArticle first published online: 20 MAY 2010 Abstract Measuring distances to galaxies, determining their chemical composition, investigating the nature of their stellar populations and the absorbing properties of their interstellar medium are fundamental activities in modern extragalactic astronomy helping to understand the evolution of galaxies and the expanding universe. The optically brightest stars in the universe, blue supergiants of spectral A and B, are unique tools for these purposes. With absolute visual magnitudes up to MV , -9.5 they are ideal to obtain accurate quantitative information about galaxies through the powerful modern methods of quantitative stellar spectroscopy. The spectral analysis of individual blue supergiant targets provides invaluable information about chemical abundances and abundance gradients, which is more comprehensive than the one obtained from HII regions, as it includes additional atomic species, and which is also more accurate, since it avoids the systematic uncertainties inherent in the strong line studies usually applied to the HII regions of spiral galaxies beyond the Local Group. Simultaneously, the spectral analysis yields stellar parameters and interstellar extinction for each individual supergiant target, which provides an alternative very accurate way to determine extragalactic distances through a newly developed method, called the Flux-weighted Gravity,Luminosity Relationship (FGLR). With the present generation of 10 m-class telescopes these spectroscopic studies can reach out to distances of 10 Mpc. The new generation of 30 m-class telescopes will allow to extend this work out to 30 Mpc, a substantial volume of the local universe (© 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source] Influence of selected factors on the dietary compositions of three targeted and co-occurring temperate species of reef fishes: implications for food partitioningJOURNAL OF FISH BIOLOGY, Issue 6 2010M. E. Platell The dietary compositions of three medium to large targeted fish species, which co-occur over reefs in temperate waters of south-western Australia, were determined. These data were then used to ascertain statistically the extent to which body size, season and habitat influence the diets of these species and the degree to which food resources were partitioned among and within those species, and thus reduced the potential of interspecific and intraspecific competition. On the west coast, Bodianus frenchii (Labridae) and Epinephelides armatus (Serranidae) spent their whole life over prominent limestone reefs, as did Glaucosoma hebraicum (Glaucosomatidae) in all but juvenile life, when it lived over low-relief, limestone substrata. The dietary composition of each species changed with increasing body size, which, in G. hebraicum, was particularly pronounced at c. 300 mm total length (LT) and therefore at the size when this species shifts habitat. When the three species co-occurred over the same reefs, their dietary compositions were significantly different, with that of B. frenchii being by far the most discrete, reflecting a far greater contribution by sedentary taxa. Thus, the diet of B. frenchii was distinguished from those of the other two species in containing substantial volumes of bivalve and gastropod molluscs and echinoid echinoderms and essentially no teleosts. Although the diets of G. hebraicum and particularly E. armatus were dominated by teleosts, and especially for larger individuals, the former species ingested greater volumes of cephalopods and small crustaceans. The pointed jaws of B. frenchii, with their forwardly directed and interlocking anterior incisors, are ideally adapted for biting and retaining their invertebrate prey, which are attached to or reside within reef crevices. In contrast, the mouths of G. hebraicum and E. armatus are broader and rounder and contain numerous small, slender and inward-pointing teeth. These teeth, in conjunction with prominent backward-curved canines in E. armatus, facilitate the capture and retention of fish prey. Observations in situ indicate that G. hebraicum is a suction feeder, while E. armatus is predominantly a ram feeder. Although reef environments on the west and south coasts differ, the diet of B. frenchii on these coasts differed only slightly. Interspecific differences in diet, combined with size-related changes in dietary compositions and the occupation of different habitats by juvenile and adult G. hebraicum, reduce the potential for competition for food resources among and within B. frenchii, G. hebraicum and E. armatus and thus helps facilitate the coexistence of these species which historically have been abundant over reefs in south-western Australia. [source] | |||||||||||||