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Background Values (background + value)
Selected AbstractsMolecular analysis of mutations at the HPRT and TK loci of human lymphoblastoid cells after combined treatments with 3,-azido-3,-deoxythymidine and 2,,3,-dideoxyinosine,ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, Issue 4 2002Quanxin Meng Abstract Combinations of antiretroviral drugs that include nucleoside reverse transcriptase inhibitors (NRTIs) are superior to single-agent regimens in treating or preventing HIV infection, but the potential long-term health hazards of these treatments in humans are uncertain. In earlier studies, our group found that coexposure of TK6 human lymphoblastoid cells to 3,-azido-2,,3,-dideoxythymidine (AZT) and 2,,3,-dideoxyinosine (ddI), the first two NRTIs approved by the FDA as antiretroviral drugs, produced multiplicative synergistic enhancement of DNA incorporation of AZT and mutagenic responses in both the HPRT and TK reporter genes, as compared with single-drug exposures (Meng Q et al. [2000a]: Proc Natl Acad Sci USA 97:12667,12671). The purpose of the current study was to characterize the mutational specificity of equimolar mixtures of 100 ,M or 300 ,M AZT + ddI at the HPRT and TK loci of exposed cells vs. unexposed control cells, and to compare the resulting mutational spectra data to those previously found in cells exposed to AZT alone (Sussman H et al. [1999]: Mutat Res 429:249,259; Meng Q et al. [2000b]: Toxicol Sci 54:322,329). Molecular analyses of HPRT mutant clones were performed by reverse transcription,mediated production of cDNA, PCR amplification, and cDNA sequencing to define small DNA alterations, followed by multiplex PCR amplification of genomic DNA to define the fractions of deletion events. TK mutants with complete gene deletions were distinguished by Southern blot analysis. The observed HPRT mutational categories included point mutations, microinsertions/microdeletions, splicing-error mutations, and macrodeletions including partial and complete gene deletions. The only significant difference or shift in the mutational spectra for NRTI-treated cells vs. control cells was the increase in the frequency of complete TK gene deletions following exposures (for 3 days) to 300 ,M AZT,ddI (P = 0.034, chi-square test of homogeneity); however, statistical analyses comparing the observed mutant fraction values (measured mutant frequency × percent of a class of mutation) between control and NRTI-treated cells for each class of mutation showed that the occurrences of complete gene deletions of both HPRT and TK were significantly elevated over background values (0.34 × 10,6 in HPRT and 6.0 × 10,6 in TK) at exposure levels of 100 ,M AZT,ddI (i.e., 1.94 × 10,6 in HPRT and 18.6 × 10,6 in TK) and 300 ,M AZT,ddI (i.e., 5.6 × 10,6 in HPRT and 34.6 × 10,6 in TK) (P < 0.05, Mann,Whitney U -statistic). These treatment-related increases in complete gene deletions were consistent with the spectra data for AZT alone (ibid.) and with the known mode of action of AZT and ddI as DNA chain terminators. In addition, cotreatments of ddI with AZT led to substantial absolute increases in the mutant fraction of other classes of mutations, unlike cells exposed solely to AZT [e.g., the frequency of point mutations among HPRT mutants was significantly increased by 130 and 323% over the background value (4.25 × 10,6) in cells exposed to 100 and 300 ,M AZT,ddI, respectively]. These results indicate that, at the same time that AZT,ddI potentiates therapeutic or prophylactic efficacy, the use of a second NRTI with AZT may confer a greater cancer risk, characterized by a spectrum of mutations that deviates from that produced solely by AZT. Environ. Mol. Mutagen. 39:282,295, 2002. Published 2002 Wiley-Liss, Inc. [source] A hydrological tracer experiment with LiCl in a high mountain lakeHYDROLOGICAL PROCESSES, Issue 12 2002Hansjörg Thies Abstract The water residence time of the high mountain seepage lake Gossenköllesee (2413 m, Tyrol, Austria) was determined by measuring the flushing rate of a tracer substance. During holomixis in July 1997, when circulation occurred throughout the entire water column, a dilute lithium chloride solution was injected into the lake causing the lithium concentration to rise from the background value of 0·06 to 3·1 ,g L,1. The water residence time was derived from the exponential decline of the lithium mass in the lake between July and October holomixis. The water residence time was about 2 months, i.e. the lake volume was exchanged twice between the two periods of holomictic conditions. Copyright © 2002 John Wiley & Sons, Ltd. [source] A model for saturation correction in meteor photometryMONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 1 2010Jean-Baptiste Kikwaya ABSTRACT In order to correct for the effect of saturation on photometric measurements of meteors, we have developed a numerical model for saturation and apply it to data gathered using two generation III image intensified video systems on two nights (2008 October 31 and 2008 November 6). The two cameras were pointed in the same direction, and the aperture of one camera was set two stops below the aperture of the other. With these conditions, some meteors saturated one camera but not the other (group I); some saturated both cameras (group II); and some did not saturate either of them (group III). A model of meteor saturation has been developed which uses the image background value, angular meteor speed and the lateral width of the meteor image to simulate the true and saturated light curve of meteors. For group I meteors, we computed a saturation correction and applied it to the saturated light curve. We then compared the corrected saturated curve to the unsaturated curve from the other camera to validate the model. For group II meteors, a saturation correction is calculated and applied to both observed light curves, which have different degrees of saturation, and the corrected curves are compared. We collected 516 meteors, of which 30 were of group I, and seven of group II. For meteors in group I, an average residual of less than 0.4 mag was found between the observed unsaturated light curve and the model-corrected saturated light curve. For meteors in group II, the average residual between the two corrected light curves was 0.3 mag. For our data, the saturation correction goes from 0.5 to 1.9 mag for meteors in group I, and 1.2 to 2.5 mag for meteors in group II. Based on the agreement between the observed and modelled light curves (less than 0.4 mag over all meteors of all groups), we conclude that our model for saturation correction is valid. It can be used to extract the true luminosity of a saturated meteor, which is necessary to calculate photometric mass. Our model also demonstrates that fixed corrections to saturated meteor photometry, not accounting for background levels or angular velocities, do introduce significant error to meteor photometric analyses. [source] Logging effects on sediment flux observed in a pollen-based record of overbank deposition in a northern California catchmentEARTH SURFACE PROCESSES AND LANDFORMS, Issue 7 2005José A. Constantine Abstract A palynological approach was used to estimate overbank deposition rates in a forested catchment affected by logging. The palynological approach uses downcore variations in total fossil pollen and fossil pollen assemblage to calculate rates of overbank deposition and has a distinct advantage over radioisotopic approaches in that it is not limited by radioactive decay. Using this approach, we determined that overbank deposition rates increased over 400 per cent within years of logging events and that the increased rates persisted for less than 4 years. After logging-induced deposition peaked, overbank deposition decreased over 60 per cent relative to the pre-logging background values. The decreased deposition rates persisted for over 40 years. The immediate effect of logging in this catchment was to induce mass-wasting events in hollows that produced rapidly travelling sediment pulses. In the subsequent recovery period, reduced sediment loading occurred as a result of a reduction in the volume of sediment available for transport. The reduction in sediment load led to a reduction in overbank deposition rates until subsequent logging disturbances destabilized and emptied other hollows. Copyright © 2005 John Wiley & Sons, Ltd. [source] Molecular analysis of mutations at the HPRT and TK loci of human lymphoblastoid cells after combined treatments with 3,-azido-3,-deoxythymidine and 2,,3,-dideoxyinosine,ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, Issue 4 2002Quanxin Meng Abstract Combinations of antiretroviral drugs that include nucleoside reverse transcriptase inhibitors (NRTIs) are superior to single-agent regimens in treating or preventing HIV infection, but the potential long-term health hazards of these treatments in humans are uncertain. In earlier studies, our group found that coexposure of TK6 human lymphoblastoid cells to 3,-azido-2,,3,-dideoxythymidine (AZT) and 2,,3,-dideoxyinosine (ddI), the first two NRTIs approved by the FDA as antiretroviral drugs, produced multiplicative synergistic enhancement of DNA incorporation of AZT and mutagenic responses in both the HPRT and TK reporter genes, as compared with single-drug exposures (Meng Q et al. [2000a]: Proc Natl Acad Sci USA 97:12667,12671). The purpose of the current study was to characterize the mutational specificity of equimolar mixtures of 100 ,M or 300 ,M AZT + ddI at the HPRT and TK loci of exposed cells vs. unexposed control cells, and to compare the resulting mutational spectra data to those previously found in cells exposed to AZT alone (Sussman H et al. [1999]: Mutat Res 429:249,259; Meng Q et al. [2000b]: Toxicol Sci 54:322,329). Molecular analyses of HPRT mutant clones were performed by reverse transcription,mediated production of cDNA, PCR amplification, and cDNA sequencing to define small DNA alterations, followed by multiplex PCR amplification of genomic DNA to define the fractions of deletion events. TK mutants with complete gene deletions were distinguished by Southern blot analysis. The observed HPRT mutational categories included point mutations, microinsertions/microdeletions, splicing-error mutations, and macrodeletions including partial and complete gene deletions. The only significant difference or shift in the mutational spectra for NRTI-treated cells vs. control cells was the increase in the frequency of complete TK gene deletions following exposures (for 3 days) to 300 ,M AZT,ddI (P = 0.034, chi-square test of homogeneity); however, statistical analyses comparing the observed mutant fraction values (measured mutant frequency × percent of a class of mutation) between control and NRTI-treated cells for each class of mutation showed that the occurrences of complete gene deletions of both HPRT and TK were significantly elevated over background values (0.34 × 10,6 in HPRT and 6.0 × 10,6 in TK) at exposure levels of 100 ,M AZT,ddI (i.e., 1.94 × 10,6 in HPRT and 18.6 × 10,6 in TK) and 300 ,M AZT,ddI (i.e., 5.6 × 10,6 in HPRT and 34.6 × 10,6 in TK) (P < 0.05, Mann,Whitney U -statistic). These treatment-related increases in complete gene deletions were consistent with the spectra data for AZT alone (ibid.) and with the known mode of action of AZT and ddI as DNA chain terminators. In addition, cotreatments of ddI with AZT led to substantial absolute increases in the mutant fraction of other classes of mutations, unlike cells exposed solely to AZT [e.g., the frequency of point mutations among HPRT mutants was significantly increased by 130 and 323% over the background value (4.25 × 10,6) in cells exposed to 100 and 300 ,M AZT,ddI, respectively]. These results indicate that, at the same time that AZT,ddI potentiates therapeutic or prophylactic efficacy, the use of a second NRTI with AZT may confer a greater cancer risk, characterized by a spectrum of mutations that deviates from that produced solely by AZT. Environ. Mol. Mutagen. 39:282,295, 2002. Published 2002 Wiley-Liss, Inc. [source] Enhanced exoenzyme activities in sediments in the presence of deposit-feeding Chironomus riparius larvaeFRESHWATER BIOLOGY, Issue 9 2007PETER STIEFArticle first published online: 10 JUN 200 Summary 1. The combined effects of deposit-feeding, bioturbation and bioirrigation by benthic macrofauna on the enzymatic hydrolysis of organic matter were studied in microcosms. Chironomus riparius larvae (Insecta, Diptera) served as model macrofauna and stinging nettle leaves (Urtica dioica) were used as a detrital food source. 2. In the upper 10 mm of the sediment (the habitat of C. riparius larvae), the activities of several exoenzymes, the contents of several fractions of particulate organic matter (POM), and the concentrations of dissolved oxidants (O2, NO) were measured on a small scale. Fluorescent particles (luminophores) were used to quantify the vertical redistribution of particles within the same layer. 3. In control sediment, the addition of detrital food enhanced exoenzyme activities in the 0,2 mm layer only. In the presence of C. riparius larvae, exoenzyme activities increased to 10 mm depth. Further, the content of POM in the 0,2 mm layer was lower in the presence than in the absence of larvae, suggesting ingestion and subduction of the added detritus. After prolonged incubation without further food addition, exoenzyme activities returned close to background values in both treatments, whereas the vertical distribution of POM remained unchanged. 4. The overall penetration depth of O2 and NO into the sediment was greater in the presence than the absence of C. riparius, the differences being more pronounced after prolonged incubation. Locally high O2 and NO concentrations due to bioirrigation by C. riparius were measured deep in the sediment. Net downward transport of particles was observed only in the presence of C. riparius larvae and only at the beginning of the incubation. 5. I conclude that deposit-feeding and bioturbation by macrofauna can quickly remove freshly deposited POM from the sediment surface and transfer it to less oxygenated sites (i.e. animal guts and deep sediment layers). Bioirrigation also increases the availability of oxidants deep in the sediment. The oscillation of oxidant supply to POM particles by ingestion,egestion, burial and re-burial, and the intermittent bioirrigation of subsurface sediment, is probably the cause of the increased rate of organic matter hydrolysis, the rate-limiting step in mineralization. [source] Permeability of the continental crust: dynamic variations inferred from seismicity and metamorphismGEOFLUIDS (ELECTRONIC), Issue 1-2 2010S. E. INGEBRITSEN Geofluids (2010) 10, 193,205 Abstract The variation of permeability with depth can be probed indirectly by various means, including hydrologic models that use geothermal data as constraints and the progress of metamorphic reactions driven by fluid flow. Geothermal and metamorphic data combine to indicate that mean permeability (k) of tectonically active continental crust decreases with depth (z) according to log k , ,14,3.2 log z, where k is in m2 and z in km. Other independently derived, crustal-scale k,z relations are generally similar to this power-law curve. Yet there is also substantial evidence for local-to-regional-scale, transient, permeability-generation events that entail permeabilities much higher than these mean k,z relations would suggest. Compilation of such data yields a fit to these elevated, transient values of log k , ,11.5,3.2 log z, suggesting a functional form similar to that of tectonically active crust, but shifted to higher permeability at a given depth. In addition, it seems possible that, in the absence of active prograde metamorphism, permeability in the deeper crust will decay toward values below the mean k,z curves. Several lines of evidence suggest geologically rapid (years to 103 years) decay of high-permeability transients toward background values. Crustal-scale k,z curves may reflect a dynamic competition between permeability creation by processes such as fluid sourcing and rock failure, and permeability destruction by processes such as compaction, hydrothermal alteration, and retrograde metamorphism. [source] GEOCHEMICAL AND GEOPHYSICAL ANOMALIES AT THE ZDANICE OIL- AND GASFIELD, SE CZECH REPUBLICJOURNAL OF PETROLEUM GEOLOGY, Issue 1 2008M. Matolin Oil at the Zdanice field in the SE Czech Republic is reservoired at a depth of 900 m in weathered Precambrian granitoids and Lower Miocene sandstones and conglomerates. Tertiary claystones, siltstones and sandstones form the top seal. Surface gamma-ray spectrometry carried out along two profiles across the field (6,880 m and 8,335 m in length, respectively) found perceptible decreases of K, U, and Th concentrations relative to background values. This can be explained in terms of enhanced leaching of these radionuclides by groundwater acids derived from hydrocarbons. Observed increases in radon and thoron in soil gas above the oil-producing zone at Zdanice may be caused by enhanced gas emanation from U- and Th-bearing minerals decomposed by groundwater acids. Laboratory analyses of 444 samples collected from 18 shallow boreholes (20 m deep) pointed to a trend of decreasing K, U and Th concentrations above the oil-producing zone at Zdanice. Decreases in rock porosity and organic carbon content were also noted together with an increase in mineralogical density, magnetic susceptibility and sulphur content. Petrophysical analyses of 330 samples from deep boreholes showed lower values of K and U in samples from oil-producing wells. These observations of minor petrophysical alterations in rocks overlying the Zdanice oilfield may be characteristic and may be applicable to hydrocarbon exploration operations elsewhere. [source] Chemical identification of comet 81P/Wild 2 dust after interacting with molten silica aerogelMETEORITICS & PLANETARY SCIENCE, Issue 8 2009Frans J. M. Rietmeijer The elements in this aerogel background were assimilated in non-vesicular and vesicular glass with the numerous nanometer Fe-Ni-S compound inclusions. After correction for the background values, the chemical data show that this piece of comet Wild 2 dust was probably an aggregate of small (<500 nm) amorphous ferromagnesiosilica grains with many tiny Fe,Ni-sulfide inclusions plus small Ca-poor pyroxene grains. This distinctive Fe- and CI-normalized element distribution pattern is found in several Stardust allocations. It appears to be a common feature in glasses of quenched aerogel melts but its exact nature is yet to be established. [source] Monitoring pollution by proton-transfer-reaction mass spectrometry during paediatric anaesthesia with positive pressure ventilation via the laryngeal mask airway or uncuffed tracheal tubeANAESTHESIA, Issue 7 2002J. Rieder Summary Twenty children aged 2,66 months were randomly allocated for airway management with either the laryngeal mask airway or uncuffed tracheal tube using intermittent positive pressure ventilation with a tidal volume of 8 ml.kg,1 and a respiratory rate adjusted to maintain end-expiratory carbon dioxide concentration at 5.3 kPa. Induction was with fentanyl/propofol and maintenance was with sevoflurane 2.5% in oxygen/air. The airway device was removed when the patients were awake and the patients were transferred to the postanaesthesia care unit 10 min later. Air was sampled from a point 1.5 m above the floor at a location remote from the ventilation outlet and analysed using a proton-transfer-reaction mass spectrometer capable of continuous trace gas analysis at the parts per billion volume (ppbv) level. The concentration of sevoflurane was recorded every minute during three consecutive phases: for 5 min before the introduction of sevoflurane (background); after introduction of sevoflurane until removal of the airway device (intra-operative); and every minute after removal until the concentration returned to background levels. Median (interquartile range [range]) intra-operative sevoflurane concentrations were 200,400 times higher than background values for the laryngeal mask airway 1 (1,2 [0,3]) ppbv vs. 404 (278,523 [83,983]) ppbv, respectively, and the tracheal tube 2 (1,3 [0,5]) ppbv vs. 396 (204,589 [107,1735]) ppbv (both p <,0.0001), and returned to background values within 5 min of removal. There were no differences in sevoflurane concentration between devices intra-operatively or after removal. The performance of the proton-transfer-reaction mass spectrometer was identical at the start and end of the 30-day study. We conclude that peri-operative sevoflurane concentration in a modern operating theatre is similar for the laryngeal mask airway and the uncuffed tracheal tube in paediatric patients receiving intermittent positive pressure ventilation. Intra-operative sevoflurane concentrations are five times lower than occupational safety limit requirements, and 1000 times lower 5 min after removal of the airway device with the patient awake. The proton-transfer-reaction mass spectrometer has potential for monitoring air quality in the operating theatre. [source] Research on Potential Fertilization of Coal Gangue in the Weibei Coalfield, ChinaACTA GEOLOGICA SINICA (ENGLISH EDITION), Issue 3 2008WANG Tong Abstract: Based on the systematic analysis of the coal gangue in Weibei Coalfield, such as petrologic characteristics, chemical composition, nutrient elements, deleterious elements, and the transformation, and compared with the soil element content background values of Loess Plateau and national harmful materials controlling standards, we conclude that the coal gangue in the Weibei Coalfield has huge potential to be used as clay fertilizer. [source] | ||||||||||||||||