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Skin Squamous Cell Carcinoma (skin + squamous_cell_carcinoma)

Distribution by Scientific Domains
Medical Sciences100%
Distribution within Medical Sciences
Dermatology50%

Selected Abstracts

Distinct progression-associated expression of tumor and stromal MMPs in HaCaT skin SCCs correlates with onset of invasion

INTERNATIONAL JOURNAL OF CANCER, Issue 10 2009
Silvia Vosseler
Abstract Matrix metalloproteinases (MMPs) are critically involved in tumor invasion and metastasis. However, failure of broad spectrum MMP inhibitors in clinical trials emphasizes the need for detailed analyses of the specific role of different MMPs in tumor malignancy. Using HaCaT-keratinocyte clones representing distinct stages in skin squamous cell carcinoma (SCC) progression, we demonstrate the expression of specific tumor and stroma-derived MMPs with the onset and maintenance of tumor invasion. Although MMP-9-positive leukocytes are present in benign and malignant tumor transplants at the onset of stromal activation and angiogenesis, mRNA expression of stroma-derived MMP-9 as well as MMP-2, ,13 and ,14 is exclusively found in enhanced malignant tumor transplants. Their expression initiates with the onset of invasion, whereas being absent in early noninvasive stages of malignant transplants. In addition, a high expression of tumor-derived MMP-1, ,2 and ,14 contributes to malignant and invasive tumor growth. However, stroma-derived MMP-3 is exclusively restricted to very late-stage invasive and malignant transplants. The functional contribution of these proteases to invasive growth is supported by the gelatinolytic activity in the tumor transplants that again initiates with the onset of invasive growth suggesting a crucial role of MMP-2, ,9, ,13 and ,14 for the establishment of a reactive stroma that promotes tumor invasion. These data demonstrate a complex cooperation of distinct tumor and stroma-derived MMPs in the establishment of malignant tumors and provide the basis for a more specific use of highly selective MMP inhibitors during distinct stages of tumor progression. © 2009 UICC [source]

Expression of minichromosome maintenance 5 protein in proliferative and malignant skin diseases

INTERNATIONAL JOURNAL OF DERMATOLOGY, Issue 11 2007
Houjun Liu
Background, The entire minichromosome maintenance (MCM) family (MCM2,7) play roles in the initiation and elongation of DNA replication. Many studies have demonstrated that MCM proteins may be better indicators of a wide variety of proliferative or cancer cells in malignant tissues. Objectives, To characterize the pattern and frequency of MCM5 expression in proliferative and malignant skin diseases in comparison with those of proliferating cell nuclear antigen (PCNA). Methods, Twelve normal skin specimens, 12 specimens of psoriasis, 21 specimens of bowenoid papulosis (BP), 16 specimens of Bowen's disease (BD), 38 specimens of skin squamous cell carcinoma (SCC), and 11 specimens of basal cell carcinoma (BCC) were subjected to immunohistochemical staining for MCM5 and PCNA. Results, MCM5 protein was expressed in the lower layers of epidermis in psoriasis, while MCM5 protein were present throughout the tumor cells in BP, BD, and moderately/poorly differentiated SCC. MCM5 protein was preferentially expressed in the periphery of well-differentiated SCC or bigger nests of BCC, although some small nests of BCC seemingly showed diffuse staining patterns. The percentages of MCM5-positive cells were 15.7% in normal skin, 21.8% in psoriasis, 75.9% in BP, 83.8% in BD, 63.5% in well-differentiated SCC, 77.5% in moderately differentiated SCC, 79.8% in poorly differentiated SCC, and 21.2% in BCC in average. Well-differentiated SCC showed a significantly lower percentage of positive cells than did moderately differentiated SCC or poorly differentiated SCC. MCM5 staining basically show a similar staining pattern to that of PCNA, but more cells tended to be stained with MCM5 than with PCNA. Conclusions, Our results demonstrate pattern and frequency of MCM5 expression in various skin diseases and suggest that MCM5 may be a useful marker to detect cell proliferation in skin tissue sections. [source]

Novel initiation genes in squamous cell carcinomagenesis: A role for substrate-specific ubiquitylation in the control of cell survival

MOLECULAR CARCINOGENESIS, Issue 8 2007
Amador Albor
Abstract The study of experimental epidermal carcinogenesis offers several advantages over other epithelial carcinogenesis models, including easy accessibility and a database of research findings spanning over a century. Our studies make use of a clonal in vitro/in vivo keratinocyte carcinogenesis model with low frequency of ras mutation and derivative clonal-initiated lineages with distinct tumor fate. Analysis of this model has yielded candidate genes involved in the stages of initiation and tumorigenic progression, and has revealed novel roles for ubiquitylation in transcriptional control of survival and apoptotic pathways during the early stages of carcinogenesis. The expression of a recently described E3-ubiquitin ligase, Trim32, is elevated during initiation, and ectopic expression of Trim32 confers extended survival in response to terminal differentiation and ultraviolet light (UV) B/TNF-, death signals. Trim32 binds and ubiquitylates Piasy, controlling its stability and accumulation. Piasy is a SUMOylation factor involved in the control of apoptosis, senescence, and NF-,B activation. NF-,B is a survival factor for keratinocytes in response to UV irradiation, the main carcinogenic stimulus for the epidermis. Piasy inhibits NF-,B activity, and promotes keratinocyte apoptosis in response to UV and TNF-,. In human skin squamous cell carcinoma (SCC) samples, we found an inverse correlation between Trim32 and Piasy expression supporting a role for Trim32,Piasy interaction in human epidermal carcinogenesis. Our hypothesis is that increased expression of Trim32 may enhance epidermal carcinogenesis, by increasing the threshold of NF-,B activity through Piasy downmodulation. © 2007 Wiley-Liss, Inc. [source]

Lower Lip Squamous Cell Carcinoma in Disabling Pansclerotic Morphea of Childhood

PEDIATRIC DERMATOLOGY, Issue 1 2009
Ivaylo Petrov M.D.
As DPMC is extremely rare, its association with skin squamous cell carcinoma (SCC) is rarer still. To our knowledge there are only two cases of SCC in patients with DPMC that developed within areas of chronic skin ulceration. We report the first case of lower lip squamous cell carcinoma arising in a young woman with DPMC and discuss the carcinogenic pathway that may have led to its occurrence. [source]