INTERNATIONAL JOURNAL OF OSTEOARCHAEOLOGY, Issue 5 2002
Vedat Onar
Abstract A total of 15 dog skulls and a number of bones have been discovered in two burial chambers dating from 1,000 BC in the necropolises of the Van-Yoncatepe Castle in eastern Turkey. The finds were a dog skeleton in burial chamber M5 and 14 dog skulls and plenty of bones in burial chamber M6. It was determined on examination that the skeleton found in burial chamber M5 was an 11,12-year female dog, and that the skulls unearthed from burial chamber M6 belonged to dogs with an age range of seven to eight months to 12,13 years. In the latter chamber, however, except for one skull whose sex could not be determined, one was ascertained to belong to a female dog and the rest to males. Calculations of 20 different indices and ratios were made on the skulls. This showed that these skulls were of the dolichocephalic type. It was observed that there were signs of a widespread periodontal disease and alveolar recession. Enamel hypoplasia and abscess chambers detected on the teeth, and deformations observed in the hard palate were evidence enough to suggest that these were undernourished dogs. There were also some facial fractures, which were noticed to have occurred before death. On the other hand, examination carried out on the bones revealed that the dogs were of medium size, and that they were likely to have had a withers height of 50,55 cm. The data obtained from both the burial chambers brought to light the fact that these dogs were more of the hunting or working types. Copyright © 2002 John Wiley & Sons, Ltd. [source]

Skeletal and Pigmentation Defects following Retinoic Acid Exposure in Larval Summer Flounder, Paralichthys dentatus

JOURNAL OF THE WORLD AQUACULTURE SOCIETY, Issue 3 2007
Gabriela M. Martinez
Supplementation of larval diets with vitamin A (VA) is routinely and successfully used to stimulate pigmentation development in hatchery-reared flatfishes. However, excess dietary VA can lead to high levels of its metabolite retinoic acid (RA) and has been associated with the occurrence of skeletal deformities, presumably via RA toxicity. We reared summer flounder larvae, Paralichthys dentatus, in water containing 0- to 20-nM RA to assess its effects on postmetamorphic pigmentation and on skeletal development. RA exposure disrupted pigmentation development: treated tanks had a smaller percentage of normally pigmented fish than did controls, with increased numbers of both hypo- and hyperpigmented individuals. Exposure also affected the development of several skeletal features: RA treatment correlated with a significant increase in the severity of defects in jaws, fins, hypurals, and vertebrae compared with control groups. [source]

INHABITANTS OF A SACRED TOWNSCAPE: An Archaeological and Osteological Analysis of Skeletal Remains from Late Viking Age and Medieval Sigtuna, Sweden

ACTA ARCHAEOLOGICA, Issue 2 2005
Anna Kjellström
First page of article [source]

Oversight and Delegation in Corporate Governance: deciding what the board should decide

CORPORATE GOVERNANCE, Issue 1 2006
Michael Useem
American boards of directors increasingly treat their delegation of authority to management as a careful and self-conscious decision. Numerically dominated by non-executives, boards recognize that they cannot run the company, and many are now seeking to provide stronger oversight of the company without crossing the line into management. Based on interviews with informants at 31 major companies, we find that annual calendars and written protocols are often used to allocate decision rights between the board and management. Written protocols vary widely, ranging from detailed and comprehensive to skeletal and limited in scope. While useful, such calendars and protocols do not negate the need for executives to make frequent judgement calls on what issues should go to the board and what should remain within management. Executives still set much of the board's decision-making agenda, and despite increasingly asserting their sovereignty in recent years, directors remain substantially dependent upon the executives' judgement on what should come to the board. At the same time, a norm is emerging among directors and executives that the latter must be mindful of what directors want to hear and believe they should decide. [source]

Chicken gizzard filamin, retina filamin and cgABP260 are respectively, smooth muscle-, non-muscle- and pan-muscle-type isoforms: Distribution and localization in muscles

CYTOSKELETON, Issue 4 2005
Kazuyo Ohashi
Abstract We determined the full cDNA sequences of chicken gizzard filamin and cgABP260 (chicken gizzard actin-binding protein 260). The primary and secondary structures predicted by these sequences were similar to those of chicken retina filamin and human filamins. Like mammals, chickens have 3 filamin isoforms. Comparison of their amino acid sequences indicated that gizzard filamin, retina filamin, and cgABP260 were the counterparts of human FLNa (filamin a), b, and c, respectively. Antibodies against the actin-binding domain (ABD) of these 3 filamin isoforms were raised in rabbits. Using immunoabsorption and affinity chromatography, we prepared the monospecific antibody against the ABD of each filamin. In immunoblotting, the antibody against the gizzard filamin ABD detected a single band in gizzard, but not in striated muscles or brain. In brain, only the antibody against the retina filamin ABD produced a strong single band. The antibody against the cgABP260 ABD detected a single peptide band in smooth, skeletal, and cardiac muscle. In immunofluorescence microscopy of muscular tissues using these antibodies, the antibody against the gizzard filamin ABD only stained smooth muscle cells, and the antibody against the retina filamin ABD strongly stained endothelial cells of blood vessels and weakly stained cells in connective tissue. The antibody against the cgABP260 ABD stained the Z-lines and myotendinous junctions of breast muscle, the Z-lines and intercalated disks of cardiac muscle, and dense plaques of smooth muscle. These findings indicate that chicken gizzard filamin, retina filamin, and cgABP260 are, respectively, smooth muscle-type, non-muscle-type, and pan-muscle-type filamin isoforms. Cell Motil. Cytoskeleton 61:214,225, 2005. © 2005 Wiley-Liss, Inc. [source]

Dystrophin upregulation in pressure-overloaded cardiac hypertrophy in rats

CYTOSKELETON, Issue 1 2003
Masato Maeda
Abstract Dystrophin is a cytoskeletal protein localized to the sarcolemma of skeletal and cardiac muscle, and neurons. We have recently demonstrated that a significant cardiac damage including myocytes injury, inflammation, and fibrosis, was found in dystrophin-deficient myocardium during pressure overload [Kamogawa et al., 2001: Cardiovasc Res 50:509,515]. However, little is known about how the cardiac sarcolemmal cytoskeleton produces qualitative and quantitative changes in response to pressure overload. Accordingly, we investigated dystrophin gene expression and protein accumulation during cardiac hypertrophy. Cardiac hypertrophy was produced by banding of the abdominal aorta of rats. Total RNA from the left ventricle of the heart was used for a quantitative reverse transcription-polymerase chain reaction (RT-PCR). Dystrophin mRNA expression significantly increased by 33 ± 18% at 1 day (P < 0.05) and 45 ± 19% at 2 days (P < 0.01) after banding, while G3PDH mRNA showed no significant change. RT-PCR for dystrophin tissue-specific exon 1 revealed that only muscle type promoter, but not non-muscle type promoter (brain and Purkinje-cell type), was activated immediately after banding. Immunohistochemistry for dystrophin showed intense cellular membrane staining with an increase in the perimeter of the myocytes by 14% at 3 days (46.3 ,m, P < 0.01) and 19% at 7 days (51.2 ,m, P < 0.01) after banding. Western blotting also showed dystrophin protein increased by 14 ± 6% at 2 days (P < 0.05) and by 32 ± 10% at 3 days (P < 0.01) after aortic banding. In conclusion, upregulation of dystrophin mRNA expression and protein accumulation occurs in response to cardiac hypertrophy. These data and the vulnerability of dystrophin-deficient myocardium to pressure overload suggest that dystrophin could play an important role in maintaining the integrity of the sarcolemma. Cell Motil. Cytoskeleton 55:26,35, 2003. © 2003 Wiley-Liss, Inc. [source]

The management of mandibular body fractures in young children

DENTAL TRAUMATOLOGY, Issue 6 2009
Dror Aizenbud
Treatment principles of this fracture type differ from that of adults due to concerns regarding mandibular growth processes and dentition development. The goal of this fracture treatment is to restore the underlying bony architecture to its preinjury position in a stable fashion as non-invasively as possible and with minimal residual esthetic and functional impairment. The management of mandibular body fractures in children depends on the fracture type and the stage of skeletal and dental development; treatment modalities range from conservative non-invasive, through closed reduction and immobilization methods to open reduction with internal fixation. Disruption of the periosteal envelope of the mandibular body may have an unpredictable effect on growth. Thus, if intervention is required closed reduction is favored. [source]

Characterization of Bves expression during mouse development using newly generated immunoreagents

DEVELOPMENTAL DYNAMICS, Issue 6 2006
Travis K. Smith
Abstract Bves (blood vessel/epicardial substance) is a transmembrane protein postulated to play a role in cell,cell interaction/adhesion. It was independently isolated by two groups as a gene product highly enriched in the developing heart. Disagreement exists about its expression during development. Most notably, the expression of Bves in non-muscle cells is disputed. Determining the expression profile of Bves is a critical initial step preceding the characterization of protein function in development and in the adult. We have generated new monoclonal antibodies against mouse Bves and used these immunoreagents to elucidate Bves expression in development. As expected, we detect Bves in myocytes of the developing heart throughout development. In addition, skeletal and smooth muscle cells including those of the coronary system express Bves. Finally, specific, but not all, epithelial derivatives of the three germ layers are stained positively with these monoclonal antibodies. Protein expression in cultured epithelial and muscle cell lines corroborate our in vivo findings. Taken together, these results demonstrate the expression of Bves in a wide range of epithelial and muscle cells during mouse embryogenesis and indicate a broad function for this protein in development, and show that these newly generated reagents will be invaluable in further investigation of Bves. Developmental Dynamics 235:1701,1708, 2006. © 2006 Wiley-Liss, Inc. [source]

Cell fate and timing in the evolution of neural crest and mesoderm development in the head region of amphibians and lungfishes

ACTA ZOOLOGICA, Issue 2009
Rolf Ericsson
Abstract Our research on the evolution of head development focuses on understanding the developmental origins of morphological innovations and involves asking questions like: How flexible (or conserved) are cell fates, patterns of cell migration or the timing of developmental events (heterochrony)? How do timing changes, or changes in life history affect head development and growth? Our ,model system' is a comparison between lungfishes and representatives from all three extant groups of amphibians. Within anuran amphibians, major changes in life history such as the repeated evolution of larval specializations (e.g. carnivory), or indeed the loss of a free-swimming larva, allows us to test for developmental constraints. Cell migration and cell fate are conserved in cranial neural crest cells in all vertebrates studied so far. Patterning and developmental anatomy of cranial neural crest and head mesoderm cells are conserved within amphibians and even between birds, mammals and amphibians. However, the specific formation of hypobranchial muscles from ventral somitic processes shows variation within tetrapods. The evolution of carnivorous larvae in terminal taxa is correlated with changes in both pattern and timing of head skeletal and muscle development. Sequence-heterochronic changes are correlated with feeding mode in terminal taxa and with phylogenetic relatedness in basal branches of the phylogeny. Eye muscles seem to form a developmental module that can evolve relatively independently from other head muscles, at least in terms of timing of muscle differentiation. [source]

Surviving atlanto-occipital dislocation

EMERGENCY MEDICINE AUSTRALASIA, Issue 4 2007
Benjamin M Bloom
Abstract Traumatic atlanto-occipital dislocation carries a significant mortality and morbidity. We present the clinical and radiological features of a case of traumatic skeletal and central nervous system disunion. Thanks to a combination of early resuscitation and luck, the patient survived an improbably severe injury to leave hospital and enjoy a degree of independent life. Such severe injuries are usually fatal and the literature on such extensive cervical disruption is often confined to postmortem evidence. [source]

D-2-Hydroxyglutaric acid inhibits creatine kinase activity from cardiac and skeletal muscle of young rats

EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, Issue 10 2003
C. G. Da Silva
Abstract Background, Tissue accumulation of high amounts of D-2-hydroxyglutaric acid (DGA) is the biochemical hallmark of the inherited neurometabolic disorder D-2-hydroxyglutaric aciduria (DHGA). Patients affected by this disease usually present hypotonia, muscular weakness, hypothrophy and cardiomyopathy, besides severe neurological findings. However, the underlying mechanisms of muscle injury in this disorder are virtually unknown. Materials and methods, In the present study we have evaluated the in vitro role of DGA, at concentrations ranging from 0·25 to 5·0 mm, on total, cytosolic and mitochondrial creatine kinase activities from skeletal and cardiac muscle of 30-day-old Wistar rats. We also tested the effects of various antioxidants on the effects elicited by DGA. Results, We first verified that total creatine kinase (CK) activity from homogenates was significantly inhibited by DGA (22,24% inhibition) in skeletal and cardiac muscle, and that this activity was approximately threefold higher in skeletal muscle than in cardiac muscle. We also observed that CK activities from mitochondrial (Mi-CK) and cytosolic (Cy-CK) preparations from skeletal muscle and cardiac muscle were also inhibited (12,35% inhibition) by DGA at concentrations as low as 0·25 mm, with the effect being more pronounced in cardiac muscle preparations. Finally, we verified that the DGA-inhibitory effect was fully prevented by preincubation of the homogenates with reduced glutathione and cysteine, suggesting that this effect is possibly mediated by modification of essential thiol groups of the enzyme. Furthermore, ,-tocopherol, melatonin and the inhibitor of nitric oxide synthase L-NAME were unable to prevent this effect, indicating that the most common reactive oxygen and nitrogen species were not involved in the inhibition of CK provoked by DGA. Conclusion, Considering the importance of creatine kinase activity for cellular energy homeostasis, our results suggest that inhibition of this enzyme by increased levels of DGA might be an important mechanism involved in the myopathy and cardiomyopathy of patients affected by DHGA. [source]

Early differentiation and migration of cranial neural crest in the opossum, Monodelphis domestica

EVOLUTION AND DEVELOPMENT, Issue 2 2003
Janet L. Vaglia
SUMMARY Marsupial mammals are born at a highly altricial state. Nonetheless, the neonate must be capable of considerable functional independence. Comparative studies have shown that in marsupials the morphogenesis of many structures critical to independent function are advanced relative to overall development. Many skeletal and muscular elements in the facial region show particular heterochrony. Because neural crest cells are crucial to forming and patterning much of the face, this study investigates whether the timing of cranial neural crest differentiation is also advanced. Histology and scanning electron microscopy of Monodelphis domestica embryos show that many aspects of cranial neural crest differentiation and migration are conserved in marsupials. For example, as in other vertebrates, cranial neural crest differentiates at the neural ectoderm/epidermal boundary and migrates as three major streams. However, when compared with other vertebrates, a number of timing differences exist. The onset of cranial neural crest migration is early relative to both neural tube development and somite formation in Monodelphis. First arch neural crest cell migration is particularly advanced and begins before any somites appear or regional differentiation exists in the neural tube. Our study provides the first published description of cranial neural crest differentiation and migration in marsupials and offers insight into how shifts in early developmental processes can lead to morphological change. [source]

Differential sensitivity to calciseptine of L-type Ca2+ currents in a ,lower'vertebrate (Scyliorhinus canicula), a protochordate (Branchiostoma lanceolatum) and an invertebrate (Alloteuthis subulata)

EXPERIMENTAL PHYSIOLOGY, Issue 6 2001
Candida M. Rogers
Voltage-dependent calcium currents in vertebrate (Scyliorhinus canicula), protochordate (Branchiostoma lanceolatum), and invertebrate (Alloteuthis subulata) skeletal and striated muscle were examined under whole-cell voltage clamp. Nifedipine (10 ,M) suppressed and cobalt (5 mM) blocked striated/skeletal muscle calcium currents in all of the animals examined, confirming that they are of the L-type class. Calciseptine, a specific blocker of vertebrate cardiac muscle and neuronal L-type calcium currents, was applied (0.2 ,M) under whole-cell voltage clamp. Protochordate and invertebrate striated muscle L-type calcium currents were suppressed while up to 4 ,M calciseptine had no effect on dogfish skeletal muscle L-type calcium currents. Our results demonstrate the presence of at least two sub-types of L-type calcium current in these different animals, which may be distinguished by their calciseptine sensitivity. We conclude that the invertebrate and protochordate L-type current sub-type that we have examined has properties in common with vertebrate ,cardiac' and ,neuronal' current sub-types, but not the skeletal muscle sub-type of the L-type channel. [source]

Localization of the mosaic transmembrane serine protease corin to heart myocytes

FEBS JOURNAL, Issue 23 2000
John D. Hooper
Corin cDNA encodes an unusual mosaic type II transmembrane serine protease, which possesses, in addition to a trypsin-like serine protease domain, two frizzled domains, eight low-density lipoprotein (LDL) receptor domains, a scavenger receptor domain, as well as an intracellular cytoplasmic domain. In in vitro experiments, recombinant human corin has recently been shown to activate pro-atrial natriuretic peptide (ANP), a cardiac hormone essential for the regulation of blood pressure. Here we report the first characterization of corin protein expression in heart tissue. We generated antibodies to two different peptides derived from unique regions of the corin polypeptide, which detected immunoreactive corin protein of approximately 125,135 kDa in lysates from human heart tissues. Immunostaining of sections of human heart showed corin expression was specifically localized to the cross striations of cardiac myocytes, with a pattern of expression consistent with an integral membrane localization. Corin was not detected in sections of skeletal or smooth muscle. Corin has been suggested to be a candidate gene for the rare congenital heart disease, total anomalous pulmonary venous return (TAPVR) as the corin gene colocalizes to the TAPVR locus on human chromosome 4. However examination of corin protein expression in TAPVR heart tissue did not show evidence of abnormal corin expression. The demonstrated corin protein expression by heart myocytes supports its proposed role as the pro-ANP convertase, and thus a potentially critical mediator of major cardiovascular diseases including hypertension and congestive heart failure. [source]

New synthesis of highly potential efficient bluish-green electroluminescent materials based on 1,3,4-oxadiazole,triazolopyridinone,carbazole derivatives for single-layer devices

HETEROATOM CHEMISTRY, Issue 2 2006
Ming-Hsiang Shin
New potential bluish-green electroluminescent materials of 1,3,4-oxadiazole,triazolopyridin- one,carbazole derivatives were synthesized and characterized for single-layer devices. Carbazole, pyridine, and triazolopyridinone were completely introduced into 1,3,4-oxadiazole skeletal to play assistant roles in controlling fundamental photolytic process due to the electron-donating nature, excellent photoconductivity, and flexible structure properties. Following the spectroscopic studies and the measurements of cyclic voltammogram, 1,3,4-oxadiazole,triazolopyridinone,carbazole derivatives were highly efficient bluish-green electroluminescent materials. © 2006 Wiley Periodicals, Inc. Heteroatom Chem 17:160,165, 2006; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/hc.20201 [source]

Molecular spectrum of SLC22A5 (OCTN2) gene mutations detected in 143 subjects evaluated for systemic carnitine deficiency,

HUMAN MUTATION, Issue 8 2010
Fang-Yuan Li
Abstract Systemic primary carnitine deficiency (CDSP) is caused by recessive mutations in the SLC22A5 (OCTN2) gene encoding the plasmalemmal carnitine transporter and characterized by hypoketotic hypoglycemia, and skeletal and cardiac myopathy. The entire coding regions of the OCTN2 gene were sequenced in 143 unrelated subjects suspected of having CDSP. In 70 unrelated infants evaluated because of abnormal newborn screening (NBS) results, 48 were found to have at least 1 mutation/unclassified missense variant. Twenty-eight of 33 mothers whose infants had abnormal NBS results were found to carry at least 1 mutation/unclassified missense variant, including 11 asymptomatic mothers who had 2 mutations. Therefore, sequencing of the OCTN2 gene is recommended for infants with abnormal NBS results and for their mothers. Conversely, 52 unrelated subjects were tested due to clinical indications other than abnormal NBS and only 14 of them were found to have at least one mutation/unclassified variant. Custom designed oligonucleotide array CGH analysis revealed a heterozygous ,1.6 Mb deletion encompassing the entire OCTN2 gene in one subject who was apparently homozygous for the c.680G>A (p.R227H) mutation. Thus, copy number abnormalities at the OCTN2 locus should be considered if by sequencing, an apparently homozygous mutation or only one mutant allele is identified. ©2010 Wiley-Liss, Inc. [source]

Advanced Material Strategies for Tissue Engineering Scaffolds

ADVANCED MATERIALS, Issue 32-33 2009
Lisa E. Freed
Abstract Tissue engineering seeks to restore the function of diseased or damaged tissues through the use of cells and biomaterial scaffolds. It is now apparent that the next generation of functional tissue replacements will require advanced material strategies to achieve many of the important requirements for long-term success. Here, we provide representative examples of engineered skeletal and myocardial tissue constructs in which scaffolds were explicitly designed to match native tissue mechanical properties as well as to promote cell alignment. We discuss recent progress in microfluidic devices that can potentially serve as tissue engineering scaffolds, since mass transport via microvascular-like structures will be essential in the development of tissue engineered constructs on the length scale of native tissues. Given the rapid evolution of the field of tissue engineering, it is important to consider the use of advanced materials in light of the emerging role of genetics, growth factors, bioreactors, and other technologies. [source]

Fly or die: the role of fat stores in the growth and development of Grey-headed Albatross Diomedea chrysostoma chicks

IBIS, Issue 2 2000
KEITH REID
Chicks of albatrosses, like other Procellariiformes, become independent at a mass similar to their parents but during growth attain a peak mass some 30% or more greater, before losing mass prior to fledging. The current views are that this high peak mass represents chicks storing fat reserves as an energy sink, or as an insurance against periodic food scarcity, or as a Consequence of natural stochastic variation in provisioning rate. We analysed growth and body composition of Grey-headed Albatross Diomedea chrysostoma chicks at Bird Island, South Georgia in 1984 and 1986, two years of very different food availability. In 1984 when overall breeding success was only 28% (the lowest in 20 years and less than halt that in 1986), chicks were significantly smaller in terms of peak mass (by 37%), primary length (by 25%), liver, lung, heart and kidney size (by 18,34%) and fat (by 75,80%) but not significantly different in terms of skeletal (tarsus, culmen, ulna, sternum) or muscle (pectoral, leg) size. Despite these differences, there were some important similarities in the patterns of growth in both years. Up to the attainment of peak mass, most of the growth of organs and of skeletal structures was completed and little fat was deposited. In the remaining part of the chick-rearing period, feather growth and acquisition of fat stores were undertaken. Thus Grey-headed Albatross chicks begin to acquire substantial fat stores only during the later part of the development period; this is contrary to the predictions of any of the existing hypotheses concerning provisioning patterns and the role of fat stores in Procellariiformes. We propose that the deposition of fat in the later stages of chick growth is an adaptation to: (a) ensure against energy demands and/or nutritional stress affecting the quality of flight feathers (many of which are not renewed for up to three years after fledging); and (b) provide an energy reserve for chicks to use in the critical period immediately after independence. [source]

Active and passive behaviors of soft tissues: Pelvic floor muscles

INTERNATIONAL JOURNAL FOR NUMERICAL METHODS IN BIOMEDICAL ENGINEERING, Issue 6 2010
M. P. M. Pato
Abstract A new active-contraction visco-elastic numerical model of the pelvic floor (skeletal) muscle is presented. Our model includes all elements that represent the muscle constitutive behavior, contraction and relaxation. In contrast with the previous models, the activation function can be null. The complete equations are shown and exactly linearized. Small verification and validation tests are performed and the pelvis is modeled using the data from the intra-abdominal pressure tests. Copyright © 2009 John Wiley & Sons, Ltd. [source]

Bizygomatic breadth determination in damaged skulls

INTERNATIONAL JOURNAL OF OSTEOARCHAEOLOGY, Issue 5 2010
C. L. Oskam
Abstract Metric and discriminant function analyses of the skull have been used successfully to determine ancestry and sex from human skeletal remains in both forensic and archaeological contexts. However, skulls are frequently discovered in damaged condition. One structure that is commonly fragmented, even when the rest of the skull is preserved, is the zygomatic arch. The bizygomatic width is an important measurement in craniometry and in forensic facial reconstruction for determining facial width; therefore we propose a simple linear regression model to predict the bizygomatic width of skulls with damaged zygomatic arches. Thirty-one adult skulls originating from the Indian sub-continent were used to measure the bizygomatic width. Then, on the same skulls, a straight steel wire was placed at the superior surface of the temporal and zygomatic origins of the zygomatic arch to simulate the zygomatic arch reconstruction on damaged skulls. These wire measurements were used to fit a simple linear regression model between the bizygomatic widths and the wire measurements, and the estimated regression model; Bizygomatic Width (bone),=,0.61,+,1.02(wire measurement), has a very high R2 value of 0.91. Hence, this model could effectively be used to predict bizygomatic widths based on wire measurements. In addition, the bizygomatic widths and wire measurements were collected from 14 New Zealand European skulls to test the ability of the regression model to determine bizygomatic widths from different ethnic groups. This model accurately predicted the bizygomatic widths in New Zealands of European origin skulls and therefore suggests that this regression model could be used for other ethnic groups. The importance of the bizygomatic width for craniometric analysis makes this regression model particularly useful for analysing archaeological samples. Furthermore, this regression line can be used in the field of forensic facial reconstruction to reconstruct damaged zygomatic arches prior to facial reconstructions. Copyright © 2009 John Wiley & Sons, Ltd. [source]

A funerary rite study of the Phoenician,Punic necropolis of Mount Sirai (Sardinia, Italy)

INTERNATIONAL JOURNAL OF OSTEOARCHAEOLOGY, Issue 2 2010
G. Piga
Abstract A recent excavation in the Phoenician,Punic necropolis of Mount Sirai, located in the southwestern part of Sardinia, Italy, has brought to light a number of tombs contextually attributed to a period from the early 6th to early 5th century BC, which is simultaneous with the beginning of the Carthago influence in Sardinia. Among the interred burials recently brought to light, the skeletal remains, sometimes of two superposed bodies, are found in a primary position and with fine anatomic connection. Some of the bones were visually stained, suggesting they were possibly subjected to fire treatment. In order to ascertain more objectively whether the bodies were subjected to burning, the bones from all the tombs were investigated by powder X-ray diffraction (XRD) and Fourier Transform infra-red (FT-IR) spectroscopy techniques. After excluding the role of important diagenetic effects, from line broadening/sharpening analysis of hydroxylapatite in the bones according to the Rietveld method, it was evaluated that the bodies were probably subjected to a temperature regime from 300 to 700°C. These data were supplemented and confirmed by an analysis of the splitting factor (SF) of apatite phosphate peaks in the infra-red spectrum of the bones. Our results indicate the existence of a rite intermediate between incineration and inhumation. This sort of ,semi-combustion', perhaps limited to the period of the early 5th century BC, appears to be peculiar just to this site. Copyright © 2008 John Wiley & Sons, Ltd. [source]

Intervertebral chondrocalcinosis: an exercise in differential diagnosis in palaeopathology

INTERNATIONAL JOURNAL OF OSTEOARCHAEOLOGY, Issue 1 2009
S. A. Mays
Abstract Intervertebral chondrocalcinosis (calcification of intervertebral discs and associated structures) may arise from a variety of causes. This work presents a discussion of intervertebral chondrocalcinosis and the identification of its most probable cause in skeletal remains, using as a case study a skeleton of an elderly male from medieval Ipswich, UK. The skeleton is examined using gross observation and radiography, and the intervertebral calcifications are subject to chemical analysis. In addition to intervertebral chondrocalcinosis (which has resulted in ankylosis of lumbo-sacral segments), lesions identified include chondrocalcinosis at some synovial joints, various soft tissue calcified bodies, and severe osteoarthritis particularly at the gleno-humeral joints. Interpretation of the results of the chemical analysis of the calcified deposits is complicated by diagenesis, but they are most consistent with apatite and/or whitlockite. Arriving at a most probable cause of the lesions in this case is difficult, but it is tentatively suggested that ochronosis may be the best diagnostic option. Copyright © 2008 John Wiley & Sons, Ltd. [source]

Using ancient DNA analysis in palaeopathology: a critical analysis of published papers, with recommendations for future work

INTERNATIONAL JOURNAL OF OSTEOARCHAEOLOGY, Issue 6 2008
C. Roberts
Abstract Despite an observable increase in the number of studies using ancient DNA analysis to diagnose disease in human remains, there remain issues to be addressed about the quality of the resulting publications. This paper describes the qualitative analysis of published papers that describe the detection of pathogenic DNA in human skeletal and mummified remains from archaeological sites. Its ultimate goal is to provide an overview of the main problematic issues in relationship to standards developed in molecular biology and to make recommendations for future work. Sixty-five papers published between 1993 and 2006 were surveyed and the quality of each was assessed using 15 criteria. Interesting results emerged. Of particular note was the high number of papers that did not acknowledge the use of even basic contamination control (90%) or procedures to validate results independently (85%). This study illustrates that attention to contamination control and authentication of results is needed in future research, if confidence in aDNA analysis in palaeopathology is to be increased. Additionally, methods of analysis must be described in published papers to ensure transparency in processes utilised to generate the data. Copyright © 2008 John Wiley & Sons, Ltd. [source]

Dental pathology and diet at Apollonia, a Greek colony on the Black Sea

INTERNATIONAL JOURNAL OF OSTEOARCHAEOLOGY, Issue 3 2008
A. Keenleyside
Abstract Dental pathology has the potential to provide insight into the composition of the diet and to reveal dietary differences based on age, sex and social status. Human skeletal remains from the Greek colonial site of Apollonia (5th to 2nd centuries BC) on the Black Sea coast of Bulgaria were analysed for various forms of dental pathology in order to: assess the prevalence of dental disease in the population; compare the dental pathology data from Apollonia with dietary data derived from ancient literary texts and from previous stable isotopic analysis of the colonists' remains; explore variations in dental disease with respect to age and sex; and compare the prevalence of dental pathology in the Apollonians with that of other Greek populations. The composition of the diet, as indicated by the dental pathology data, is consistent with the stable isotopic evidence from Apollonia and with the ancient literary texts, both of which indicate the consumption of a relatively soft, high carbohydrate diet. The higher frequency of dental caries, abscesses, calculus, and antemortem tooth loss in older adults compared with younger ones reflects the age-progressive nature of these conditions. The lack of significant sex differences in caries, abscesses, calculus and tooth loss corresponds with the stable carbon and nitrogen isotopic data derived from bone collagen, which indicate no significant sex differences in the consumption of dietary protein. In contrast, these findings conflict with the ancient literary texts, which refer to distinct dietary differences between males and females, and with the stable carbon isotopic values derived from bone carbonate, which indicate sex differences with respect to the overall diet. Despite the lack of marked sex differences in dental pathology, overall trends point to subtle dietary differences between males and females. A greater degree of tooth wear in males also hints at possible sex differences in the use of the teeth as tools. Copyright © 2007 John Wiley & Sons, Ltd. [source]

Dental trauma and antemortem tooth loss in prehistoric Canary Islanders: prevalence and contributing factors

INTERNATIONAL JOURNAL OF OSTEOARCHAEOLOGY, Issue 2 2007
J. R. Lukacs
Abstract Differential diagnosis of the aetiology of antemortem tooth loss (AMTL) may yield important insights regarding patterns of behaviour in prehistoric peoples. Variation in the consistency of food due to its toughness and to food preparation methods is a primary factor in AMTL, with dental wear or caries a significant precipitating factor. Nutritional deficiency diseases, dental ablation for aesthetic or ritual reasons, and traumatic injury may also contribute to the frequency of AMTL. Systematic observations of dental pathology were conducted on crania and mandibles at the Museo Arqueologico de Tenerife. Observations of AMTL revealed elevated frequencies and remarkable aspects of tooth crown evulsion. This report documents a 9.0% overall rate of AMTL among the ancient inhabitants of the island of Tenerife in the Canary Archipelago. Sex-specific tooth count rates of AMTL are 9.8% for males and 8.1% for females, and maxillary AMTL rates (10.2%) are higher than mandibular tooth loss rates (7.8%) Dental trauma makes a small but noticeable contribution to tooth loss among the Guanches, especially among males. In several cases of tooth crown evulsion, the dental root was retained in the alveolus, without periapical infection, and alveolar bone was in the initial stages of sequestering the dental root. In Tenerife, antemortem loss of maxillary anterior teeth is consistent with two potential causal factors: (a) accidental falls while traversing volcanic terrain; and (b) interpersonal combat, including traditional wrestling, stick-fighting and ritual combat. Steep-walled valleys (barrancos) and lava fields (malpaís) required agile locomotion and occasional vaulting with the aid of a wooden staff. Accidental falls involving facial injury may have contributed to AMTL. Traditional conflict resolution involved competitive wrestling (lucha canaria), stick-fighting (juego del palo), and ritualised contests involving manual combat. These activities made a small but recognisable impact on anterior dental trauma and tooth loss. Inter-personal behaviours of such intensity leave their mark on skeletal and dental remains, thereby providing insight into the lives and cultural traditions of the ancient Guanches. Copyright © 2006 John Wiley & Sons, Ltd. [source]

Palaeopathological and palaeogenetic study of 13 cases of developmental dysplasia of the hip with dislocation in a historical population from southern France

INTERNATIONAL JOURNAL OF OSTEOARCHAEOLOGY, Issue 1 2007
B. Mafart
Abstract Dislocation of the hip can be consecutive to developmental dysplasia of the hip that is linked to a complex set of genetic and mechanical factors. The purpose of this report is to describe 13 cases of complete dysplastic hip dislocation observed in the skeletal remains of nine women exhumed from an historical gravesite dating from the 5th to 17th centuries in southern France. Despite the size of this palaeopathological series, which is the largest study published to date, findings indicate that the prevalence of hip dislocation in this historical sample was still lower than in some French areas at the beginning of the 20th century. Mitochondrial DNA sequencing demonstrated possible kinship only between two women, i.e. one who died in the period from the 11th to 13th centuries and another who died in the period from the 16th to 17th centuries. It is suggested that the tight swaddling of young infants in France up to the end of the 19th century could have been a predisposing factor for this highly debilitating disorder. Copyright © 2006 John Wiley & Sons, Ltd. [source]

Estimating age by tooth wear of prehistoric human remains in Brazilian archaeological sites

INTERNATIONAL JOURNAL OF OSTEOARCHAEOLOGY, Issue 5 2006
R. N. Oliveira
Abstract The occlusal surfaces of 298 permanent maxillary and mandibular molar teeth of prehistoric shellfish-gatherer subjects from the Piaçaguera and Tenorio sites (4930 to 1875 BP), near the central-northern coastline of São Paulo, Brazil, were examined for classification of macro-wear stages. Molar tooth wear is an indication of masticatory activity and can be used in the estimation of age at death. The examination of visual and schematic aspects of occlusal macro-wear used a visual chart proposed by Brothwell, which includes the three superior and inferior, left and right, permanent molars. Three examiners performed the macroscopic observations twice under the same conditions. The resulting age estimates were compared with previous information of age estimated by skeletal examination. A reduced intra- and inter-observer variation was observed; all re-examinations indicated discrepancies of less than two years for the upper and lower limit of the age range estimates. The procedure was also considered consistent with the skeletal method used for age estimation of human remains excavated in Brazilian archaeological shell mounds, with a discrepancy of less than 8.22 years between the upper and lower limits of estimates by both methods. Age classification by the occlusal molar wear may be a useful tool for the classification of archaeological findings, mainly when only fragmentary skeletal remains are excavated. The current results indicate that the application of the Brothwell chart for Brazilian archaeological series presented satisfactory results of consistency, and its expanded use may represent a relevant adjunct for research. Copyright © 2006 John Wiley & Sons, Ltd. [source]

The necropolis of Vallerano (Rome, 2nd,3rd century AD): an anthropological perspective on the ancient Romans in the Suburbium

INTERNATIONAL JOURNAL OF OSTEOARCHAEOLOGY, Issue 2 2006
A. Cucina
Abstract The present study investigates the skeletal remains of individuals who were part of a Roman suburban community, in order to assess lifestyle and living conditions in the town's outskirts during the Roman Imperial age. The existence of the community was linked to the functioning of one of the many villas that surrounded the town of Rome at that time. In order to assess health, several indicators were explored, including mortality, oral pathologies and specific (cribra orbitalia) and aspecific (linear enamel hypoplasia) indicators of nutritional and physiological impairment. The sample, which probably represents the labour force of the villa, shows a high number of individuals dying in the early adult age and very few living beyond 50. Subadults were frequently affected by pathological conditions which may indicate anaemia and/or inflammations and infections, as witnessed by the frequency of cribra orbitalia. Growth was also impaired, as the individuals suffered from systemic disturbances during the early years of life that led to the formation of linear enamel hypoplasia (LEH) in their teeth. Frequency of LEH is very high, as well as its multiple occurrence through time (2.44 defects per individual) and its onset occurs from the earliest age classes. Diet, on the other hand, does not seem to have been particularly carbohydrate based. Oral pathologies are very low, which is consistent with meat consumption complementing a diet rich in low-calorific products of agriculture and seemingly low in refined carbohydrates. Copyright © 2005 John Wiley & Sons, Ltd. [source]

Diameter of the human internal acoustic meatus and sex determination

INTERNATIONAL JOURNAL OF OSTEOARCHAEOLOGY, Issue 2 2006
N. Lynnerup
Abstract We report the results of testing the diameter of the internal opening of the acoustic canal in the petrous part of the temporal bone for sex determination of skeletal remains. The method involves measuring the diameter using a suite of ordinary drills. The method is very simple and has the great advantage of utilising one of the sturdiest bone elements of the human skeleton. The method may be especially useful for the analyses of very fragmented skeletal remains or cremated bones, where the petrous bone may still be readily recognisable. The method was tested using a forensic sample of 113 left petrous bones with known sex. Intra- and inter-observer testing was also performed. We found a statistically significant difference in diameter between males and females (means: males: 3.7,mm; females: 3.4,mm; P,<,0.009). However, the low predictive value (70%) for correct sexing using two sectioning points (,<,3.0,mm,=,female; >3.5,mm,=,male) was disappointing. No additional accuracy was gained by employing both left and right petrous bones (a bilateral sample of 60 petrous bones was also tested), although left and right side diameter is highly correlated (R,=,0.778; P,=,0.0001). Copyright © 2005 John Wiley & Sons, Ltd. [source]

Use of the first rib for adult age estimation: a test of one method

INTERNATIONAL JOURNAL OF OSTEOARCHAEOLOGY, Issue 5 2005
H. Kurki
Abstract The human first rib is relatively easy to identify and is often preserved, in comparison with elements such as the fourth rib and pubic symphysis. Therefore it is potentially a valuable skeletal element for estimating age in forensic and archaeological contexts. A method of adult age estimation using the first rib (Kunos et al., 1999) is tested on a sample of known age skeletons from the J.C.B. Grant Collection (n,=,29, mean age,=,55.7 years). The high correlation coefficient (r,=,0.69) and moderate coefficient of determination (r2,=,0.47) demonstrate agreement between the known and estimated ages, suggesting that the first rib demonstates morphological changes with age. The inaccuracy and bias are high (all ages inaccuracy,=,10.4 years, bias,=,4.7 years) but comparable to several other age estimation methods in common use. Although the results are not as good for younger age categories (<,50 years: inaccuracy and bias rank ninth of nine age estimation methods), the inaccuracy and bias for the older age categories are relatively low (60,+ years inaccuracy,=,8.9 years, ranks third out of nine; bias,=,,,5.8 years, ranks first out of nine) compared with other age estimation methods. The first rib method is reasonably precise (93% of individuals fall within the limits of agreement of the mean difference between two trials). The first rib method is therefore a useful addition to the methods available for biological profile reconstructions from skeletal remains, especially if it is suspected that the remains represent an older individual. Copyright © 2005 John Wiley & Sons, Ltd. [source]