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Serum IgE Antibodies (serum + ige_antibody)
Selected AbstractsCross-reactivity among fungal allergens: a clinically relevant phenomenon?MYCOSES, Issue 2 2009Reto Crameri Summary Atopic patients suffering from allergic asthma, allergic rhinitis, or atopic eczema often have detectable levels of serum IgE antibodies to fungi. Although the association between fungal sensitisation and different forms of allergic diseases, including allergic asthma and life-threatening allergic bronchopulmonary aspergillosis, is well established, the clinical relevance of cross-reactivity among different fungal species remains largely unknown. Recent progress in molecular cloning of fungal allergens and the availability of more than 40 completely sequenced fungal genomes facilitates characterisation, cloning, and production of highly pure recombinant allergens, identification of homologous and orthologous allergens widespread among the fungal kingdom, in silico prediction, and experimental in vitro and in vivo verification of cross-reactivity between homologous pan-allergens. These studies indicate that cross-reactivity is an important component of fungal sensitisation. [source] The course of eczema in children aged 5,7 years and its relation to atopy: differences between boys and girlsBRITISH JOURNAL OF DERMATOLOGY, Issue 3 2006M. Möhrenschlager Summary Background, The role of atopy in the pathophysiology of eczema is still under debate. The concept and analyses of the nonatopic and atopic subtypes of eczema have gained increasing interest in recent studies. The course of these subtypes and differences between boys and girls have not been investigated so far. Objectives, To examine the course of nonatopic and atopic eczema in preschool children from Germany with regard to sex. Methods, Repeated cross-sectional studies were performed in 5,7-year-old preschool children from Germany between 1994 and 2000. Individuals with eczema were identified by a dermatological examination. In addition to a questionnaire, skin prick tests and analyses of serum IgE antibodies against seven and five environmental allergens, respectively, were performed. Atopy was defined by sensitization to at least one of five common aeroallergens (birch, grass and mugwort pollen, house dust mites, cat dander). In part of the study population investigations of spare time behaviour and skin function were carried out (including stratum corneum hydration and skin surface pH). Results, A total of 2693 girls and 2783 boys underwent a full dermatological examination of the skin and determination of sensitization. Among the girls, 8·7% demonstrated eczema clinically at the day of investigation in contrast to 6·1% of the boys. In girls, early onset eczema (< 2 years of age) was strongly related to atopy at age 5,7 years [odds ratio (OR) 3·7; 95% confidence interval (CI) 2·7,5·1], whereas late-onset eczema (, 2 years of age) was not (OR 1·0; 95% CI 0·7,1·5). Boys were more often atopic at the age of 5,7 years than girls (28·3% vs. 20·6%), and early and late-onset eczema were related to atopy without such a difference (OR 2·8, 95% CI 2·0,4·0; OR 1·9, 95% CI 1·3,2·8, respectively). The excess of current eczema in 5,7-year-old girls compared with boys was related to the nonatopic type. The higher susceptibility of girls in that age group to develop eczema was reflected by the skin physiological examination: even girls without eczema had significantly higher skin surface pH and lower stratum corneum hydration than boys. Additionally, questionnaire data revealed that girls more often than boys predominantly played indoors, which was associated with more eczema. Conclusions, Atopy and eczema develop differently in boys and girls. Boys are more often atopic, whereas girls suffer significantly more often from eczema without relation to atopy. The nonatopic type of eczema in preschool girls is noticed predominantly after their second birthday. Genetic and lifestyle factors may contribute to this difference. [source] Histamine release test and measurement of antigen‐specific IgE antibody in the diagnosis of allergic conjunctival diseasesJOURNAL OF CLINICAL LABORATORY ANALYSIS, Issue 2 2001Eiichi Uchio Abstract Although systemic allergic laboratory tests for the quantification of allergen‐specific serum IgE antibody have been widely used, in these tests a high titer of serum specific IgE does not necessarily indicate evidence of allergy. We evaluated the diagnostic value of the glass microfiber‐based histamine release test (HRT) using small amounts of whole blood, in 36 cases of allergic conjunctival diseases: 17 cases of allergic conjunctivitis and 19 of atopic keratoconjunctivitis. The patients were evaluated by HRT, capsulated hydrolic carrier polymer (CAP)‐RAST, and conjunctival provocation test (CPT) against ten allergens. The positive rates for all allergens were higher in CAP‐RAST than in HRT. The mean concordance of HRT with CAP‐RAST results was 0.789. The mean concordance of HRT with CPT was 0.892 and that of CAP‐RAST with CPT was 0.693. A significantly higher concordance was observed in HRT than CAP‐RAST for Japanese cedar and mite antigen. The mean sensitivity, specificity, and efficiency of HRT were higher than those of CAP‐RAST. These results indicate that CAP‐RAST is good for the screening of allergens and that HRT has an advantage in the confirmation of clinical allergens in allergic conjunctival diseases because of its high sensitivity, specificity, efficiency, and higher concordance with CPT. J. Clin. Lab. Anal. 15:71–75, 2001. © 2001 Wiley‐Liss, Inc. [source] After 6 years with Xolair; a 3-year withdrawal follow-upALLERGY, Issue 1 2010A. Nopp Abstract Background:, This study reports the clinical and immunological state of patients 3 years after a 6-year period of Xolair treatment for severe allergic asthma. Methods:, The patient's cat allergen sensitivity, measured as CD-sens, IgE and IgE- and IgG4 antibodies, was analysed and compared with asthma severity evaluated from FEV1 and a questionnaire. Results:, Three years after treatment with Xolair was stopped, 12/18 patients reported improved or unchanged asthma compared with ongoing Xolair treatment. Most of the patients were in a stable clinical condition, 16/18 had not increased nightly asthma attacks and 14/18 little or no increase in medication. The CD-sens to cat was still significantly lower (P < 0.02) than untreated patients with allergic asthma and lower than expected from their serum IgE antibody levels. Conclusion:, Most of the patients in this study had, still 3 years after closing of 6 years Xolair treatment, a surprisingly mild and stable asthma. Interestingly, the observed, considerable, downregulation of basophil allergen sensitivity, CD-sens, most likely representing mast cell allergen sensitivity, contributed to the clinical results. [source] | ||||||||||