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Separation Experiments (separation + experiment)
Selected AbstractsGas chromatographic separation of saturated aliphatic hydrocarbon enantiomers on permethylated ,-cyclodextrinCHIRALITY, Issue S1 2003Uwe J. Meierhenrich Abstract Enantiomers of chiral aliphatic hydrocarbons are generally difficult to separate because they lack functional groups to be derivatized in order to generate diastereomers. The systematic and quantitative separation of a series of branched hydrocarbon enantiomers using a chiral cyclodextrin stationary phase and a cryostat-controlled gas chromatograph is described. The use of a cryogenic system allows the improvement of separations for various chiral aliphatic hydrocarbons. The molecular cyclodextrin-based mechanism of the achieved enantiomeric separations is discussed briefly. Possible applications of this analytical technique are summarized, with special emphasis on the planned enantiomeric separation experiment on a cometary nucleus. Chirality 15:S13,S16, 2003. © 2003 Wiley-Liss, Inc. [source] Synthesis, structure, and selective separation behavior of copper-imprinted microporous polymethacrylate beadsAICHE JOURNAL, Issue 12 2009Nguyen To Hoai Abstract Metal ion-imprinted polymethacrylate beads with sizes ranging from 100 to 300 ,m were prepared by suspension polymerization for the application of selective separation of target metal ions. The metal ion contacting area of the beads was enlarged via pore formation (BET 425 m2/g) using toluene as a porogenic agent. The synthesis of the copper-imprinted porous beads was verified using FTIR, SEM, and ESCA. Separation capacity and selectivity were investigated carrying out column separation experiments. The selective adsorption behavior of the imprinted beads was significantly affected by flow rate, pH, and metal ion concentration in the solution. Adsorption of the copper ion, the template metal ion, onto the beads was highly selective, compared with other ions such as nickel and zinc, with the selective coefficients at approximately 5,10. The microporous particles possessing such high selectivity has a potential application as novel column packing materials especially requiring high selective efficiency, which is usually not achievable by commercial ion exchange resins. © 2009 American Institute of Chemical Engineers AIChE J, 2009 [source] Preparation and characterization of monodisperse superparamagnetic poly(vinyl alcohol) beads by reverse spray suspension crosslinkingJOURNAL OF POLYMER SCIENCE (IN TWO SECTIONS), Issue 1 2008Chengli Yang Abstract A novel protocol for preparing magnetic poly(vinyl alcohol) (PVA) beads by reverse spray suspension crosslinking was reported. The hydrophilic Fe3O4 nanoparticles were mixed with PVA, glutaraldehyde, and water to form aqueous phase. Then the aqueous phase was sprayed into vegetable oil by a pressure of nitrogen gas to form water in oil (W/O) suspension. The magnetic PVA beads were obtained in the presence of hydrochloric acid catalyst. It was found that the magnetic PVA beads obtained good properties when the PVA concentration was 10%, and the oil phase temperature was controlled at 40 °C. The mechanical stirring has little impact on the size of magnetic PVA beads in the process of reverse spray suspension crosslinking. The Cibacron Blue (CB) was coupled on the surface of magnetic PVA beads by surface chemical reaction. The morphology, size, and magnetic properties of the magnetic PVA beads were examined by scanning electron microscopy, laser diffraction, and vibrating sample magnetometer, respectively. Compared with the stirring method, it was found that the size of magnetic PVA beads was monodisperse and their saturation magnetization was much higher. Fourier transform infrared and X-ray photoelectron spectroscopy experimental results proved that CB molecules were covalently immobilized onto the surface of the magnetic PVA beads. Meanwhile, the protein affinity separation experiments demonstrated that the magnetic PVA beads can potentially be used as a carrier for large-scale protein separation. © 2007 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 46: 203,210, 2008 [source] Chromatographic separation of cytidine triphosphate from fermentation broth of yeast using anion-exchange cryogelJOURNAL OF SEPARATION SCIENCE, JSS, Issue 4 2008Lianghua Wang Abstract A novel separation method was developed to isolate directly cytidine triphosphate (CTP) from fermentation broth of yeast using anion-exchange supermacroporous cryogel. The anion-exchange cryogel with tertiary amine groups was prepared by graft polymerization. The breakthrough characteristics and elution performance of pure CTP in the cryogel bed were investigated experimentally and the CTP binding capacity was determined. Then the separation experiments of CTP from crude fermentation broth of yeast using the cryogel column were carried out using deionized water and 0.01 M HCl as washing buffer, respectively. The chromatographic behavior was monitored and analyzed. The purity and concentration of the obtained CTP in these processes were determined quantitatively by HPLC. The maximal purity of CTP obtained at the condition of 0.01 M HCl as washing buffer and 0.5 M NaCl in 0.01 M HCl as elution buffer reached 93%. [source] | ||||||||||