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Scaffold Materials (scaffold + material)
Selected AbstractsIn Vitro Characteristics of Surface-Modified Biphasic Calcium Phosphate/Poly(L -Lactide) BiocompositeADVANCED ENGINEERING MATERIALS, Issue 4 2010Weizhong Yang Abstract Surface-modified biphasic calcium phosphate (BCP)/poly(L -lactide) (PLLA) biocomposite is shown to have improved microstructure and mechanical properties compared to the unmodified system. In vitro biodegradation and bioactivity of the composite are investigated in simulate body fluid for up to four weeks. Weight changes of the samples and the pH changes of the SBF are recorded. Surface properties of the composite after immersion are characterized by XRD, SEM and EDX analyses. Cyto-compatibility was determined by MTT assay with L929 mouse fibroblasts. The difference of the degradation behavior between modified BCP/PLLA and the reference unmodified composite are investigated, and mBCP/PLLA composite is proved to be a better as a scaffold material. The surface formed bio-apatite layer after immersion shows the excellent bioactivity of the mBCP/PLLA composite. L929 cells show a high growth rate and proliferation, demonstrating the good cytocompatibility of mBCP/PLLA composite. [source] Evidence of innervation following extracellular matrix scaffold-mediated remodelling of muscular tissuesJOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, Issue 8 2009Vineet Agrawal Abstract Naturally occurring porcine-derived extracellular matrix (ECM) has successfully been used as a biological scaffold material for site-specific reconstruction of a wide variety of tissues. The site-specific remodelling process includes rapid degradation of the scaffold, with concomitant recruitment of mononuclear, endothelial and bone marrow-derived cells, and can lead to the formation of functional skeletal and smooth muscle tissue. However, the temporal and spatial patterns of innervation of the remodelling scaffold material in muscular tissues are not well understood. A retrospective study was conducted to investigate the presence of nervous tissue in a rat model of abdominal wall reconstruction and a canine model of oesophageal reconstruction in which ECM scaffolds were used as inductive scaffolds. Evidence of mature nerve, immature nerve and Schwann cells was found within the remodelled ECM at 28 days in the rat body wall model, and at 91 days post surgery in a canine model of oesophageal repair. Additionally, a microscopic and morphological study that investigated the response of primary cultured neurons seeded upon an ECM scaffold showed that neuronal survival and outgrowth were supported by the ECM substrate. Finally, matricryptic peptides resulting from rapid degradation of the ECM scaffold induced migration of terminal Schwann cells in a concentration-dependent fashion in vitro. The findings of this study suggest that the reconstruction of tissues in which innervation is an important functional component is possible with the use of biological scaffolds composed of extracellular matrix. Copyright © 2009 John Wiley & Sons, Ltd. [source] Mussel-Inspired Polydopamine Coating as a Universal Route to Hydroxyapatite CrystallizationADVANCED FUNCTIONAL MATERIALS, Issue 13 2010Jungki Ryu Abstract Bone tissue is a complex biocomposite material with a variety of organic (e.g., proteins, cells) and inorganic (e.g., hydroxyapatite crystals) components hierarchically organized with nano/microscale precision. Based on the understanding of such hierarchical organization of bone tissue and its unique mechanical properties, efforts are being made to mimic these organic,inorganic hybrid biocomposites. A key factor for the successful designing of complex, hybrid biomaterials is the facilitation and control of adhesion at the interfaces, as many current synthetic biomaterials are inert, lacking interfacial bioactivity. In this regard, researchers have focused on controlling the interface by surface modifications, but the development of a simple, unified way to biofunctionalize diverse organic and inorganic materials remains a critical challenge. Here, a universal biomineralization route, called polydopamine-assisted hydroxyapatite formation (pHAF), that can be applied to virtually any type and morphology of scaffold materials is demonstrated. Inspired by the adhesion mechanism of mussels, the pHAF method can readily integrate hydroxyapatites on ceramics, noble metals, semiconductors, and synthetic polymers, irrespective of their size and morphology (e.g., porosity and shape). Surface-anchored catecholamine moieties in polydopamine enriches the interface with calcium ions, facilitating the formation of hydroxyapatite crystals that are aligned to the c -axes, parallel to the polydopamine layer as observed in natural hydroxyapatites in mineralized tissues. This universal surface biomineralization can be an innovative foundation for future tissue engineering. [source] Growth of osteoblast-like cells on biomimetic apatite-coated chitosan scaffoldsJOURNAL OF BIOMEDICAL MATERIALS RESEARCH, Issue 1 2008I. Manjubala Abstract Porous scaffold materials that can provide a framework for the cells to adhere, proliferate, and create extracellular matrix are considered to be suitable materials for bone regeneration. Interconnected porous chitosan scaffolds were prepared by freeze-drying method, and were mineralized by calcium and phosphate solution by double-diffusion method to form nanoapatite in chitosan matrix. The mineralized chitosan scaffold contains hydroxyapatite nanocrystals on the surface and also within the pore channels of the scaffold. To assess the effect of apatite and porosity of the scaffolds on cells, human osteoblast (SaOS-2) cells were cultured on unmineralized and mineralized chitosan scaffolds. The cell growth on the mineralized scaffolds and on the pure chitosan scaffold shows a similar growth trend. The total protein content and alkaline phosphatase enzyme activity of the cells grown on scaffolds were quantified, and were found to increase over time in mineralized scaffold after 1 and 3 weeks of culture. The electron microscopy of the cell-seeded scaffolds showed that most of the outer macropores became sealed off by a continuous layer of cells. The cells spanned around the pore wall and formed extra cellular matrix, consisting mainly of collagen in mineralized scaffolds. The hydroxyproline content also confirmed the formation of the collagen matrix by cells in mineralized scaffolds. This study demonstrated that the presence of apatite nanocrystals in chitosan scaffolds does not significantly influence the growth of cells, but does induce the formation of extracellular matrix and therefore has the potential to serve for bone tissue engineering. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2008 [source] Skeletal tissue engineering using silk biomaterialsJOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, Issue 2-3 2008Ana C. MacIntosh Abstract Silks have been proposed as potential scaffold materials for tissue engineering, mainly because of their physical properties. They are stable at physiological temperatures, flexible and resist tensile and compressive forces. Bombyx mori (silkworm) cocoon silk has been used as a suture material for over a century, and has proved to be biocompatible once the immunogenic sericin coating is removed. Spider silks have a similar structure to silkworm silk but do not have a sericin coating. This paper provides a general overview on the use of silk protein in biomaterials, with a focus on skeletal tissue engineering. Copyright © 2008 John Wiley & Sons, Ltd. [source] | ||||||||||