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Same Dimension (same + dimension)
Selected AbstractsIdentification and separation of DNA-hybridized nanocolloids by Taylor cone harmonicsELECTROPHORESIS, Issue 18 2009Xinguang Cheng Abstract A rapid (minutes) electrospray bead-based DNA hybridization detection technique is developed by spraying a mixture of hybridized and unhybridized silica nanocolloids. With proper far-field control by external electrodes, the trajectory of the ejected nanobeads from the electrospray is governed by specific harmonics of the Laplace equation, which select discrete polar angles along well-separated field maxima near the conducting Taylor cone. Due to Rayleigh fission and evaporation, beads of different size acquire different total charge after ejection and suffer different normal electrophoretic displacement such that they are ejected along well-separated field maxima and are deposited in distinct rings on an intersecting plane. As the hybridized DNA is of the same dimension as that of the nanocolloid, the nanocolloids are hence easily differentiated from the unhybridized ones. This technique is highly specific as the high shear stress in the microjet shears away any non-specifically bound DNA from the nanocolloid surface. [source] Kinetic studies on aminopeptidase M-mediated degradation of human hemorphin LVV-H7 and its N -terminally truncated productsJOURNAL OF PEPTIDE SCIENCE, Issue 7 2008Harald John Abstract The human hemorphin LVV-H7 belongs to the class of µ-opiod receptor-binding peptides, which also exhibits significant affinity to insulin-regulated aminopeptidase (IRAP) thereby affecting IRAP inhibition. The inhibitory potency towards IRAP is of pharmaceutical interest for the treatment of Alzheimer's disease. Consecutive N -terminal cleavage of the first two amino acid residues of LVV-H7 affects a drastic increase of the binding affinity (V-H7) but ultimately leads to its complete abolition after cleavage of the next amino acid residue (H7). Therefore, we investigated LVV-H7 truncation by aminopeptidase M (AP-M) identified as a LVV-H7 degrading enzyme potentially regulating hemorphin activity towards IRAP in vivo. Using a selective quantitative multi-component capillary zone electrophoretic method (CZE-UV), we analyzed the AP-M-mediated subsequent proteolysis of the hemorphins LVV-H7 (L32 -F41), VV-H7 (V33 -F41), and V-H7 (V34 -F41) in vitro. Incubations were carried out with synthetic hemorphins applied as single substrates or in combination. Maximum velocities (Vmax), catalytic constants (turnover numbers, kcat), and specific enzyme activities (EA) were calculated. L32 cleavage from LVV-H7 happens more than two-times faster (kcat: 140 min,1 ± 9%, EA: 1.0 U/mg ± 9%) than V33 cleavage from VV-H7 (kcat: 61 min,1 ± 10%, EA: 0.43 U/mg ± 10%) or V32 deletion from V-H7 (kcat: 62 min,1 ± 8%, EA: 0.46 U/mg ± 8%). In contrast, we showed that H7 (Y35 -F41) was neither degraded by porcine AP-M nor did it act as an inhibitor for this enzyme. Determined turnover numbers were in the same dimension as those reported for dynorphin degradation. This is the first time that AP-M-mediated truncation of natural underivatized LVV-H7 and its physiological metabolites was analyzed to determine kinetic parameters useful for understanding hemorphin processing and designing hemorphin-derived drug candidates. Copyright © 2008 European Peptide Society and John Wiley & Sons, Ltd. [source] Residual stress analysis of an autofrettaged compound cylinder under machining processMATERIALWISSENSCHAFT UND WERKSTOFFTECHNIK, Issue 3 2009E.-Y. Lee Autofrettage; Schrumpfpassung; Verbundzylinder; Bearbeitungsprozess Abstract The autofrettage process is used to manufacture pressure vessels and cylinders that can withstand high internal pressure. A multi-layered cylinder was assembled by a shrink fit. Autofrettaged compound cylinder can resist higher internal pressure than a thick cylinder having the same dimension and extend its life time. Inner and outer surfaces of the autofrettaged compound cylinder have to be manufactured into exact dimensions. The distribution of residual stress can change after the machining process. The machining procedure of inner and outer surfaces also affects the distribution of residual stress as a function of the machining procedure. In this study, the distribution of residual stresses of an autofrettaged compound cylinder as machining procedure was investigated using analytical and numerical analyses. [source] Aerated autoclaved concrete: Stochastic structure model and elastic propertiesPROCEEDINGS IN APPLIED MATHEMATICS & MECHANICS, Issue 1 2005Ilja Kadaschewitsch Aerated autoclaved concrete (AAC) is a modern and important construction material, whose elastic properties are primarily defined by its porosity. The possibility to predict elastic properties of AAC based on the voids distribution is very important. The report describes simulations of the mechanical properties of AAC, based on a stochastic-geometric model of its structure. The model is the well-known "cherry-pit" model, which presents a random system of partially overlapping spheres. In the mechanical analysis the solid phase is approximated by a network model with the help of the so-called radical tessellation with respect to the hard spheres of the "cherry-pit" model. The network edges are modelled in ANSYS as 3D beams. In this approach, the discretized elements (the edges) have in distinction to FE calculations with small polyhedral same dimension as the air voids and so the numerical costs can be drastically reduced. The FE simulations calculate the elastic constants and energy concentrations, which are responsible for the material failures, in large samples. Comparisons with fracture tests showed good matching between simulations and experiments. (© 2005 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source] A power function model for the basic geometry of solution dolines: considerations from the classical karst of south-central SloveniaEARTH SURFACE PROCESSES AND LANDFORMS, Issue 3 2006France Abstract Solution doline geometry is generally too complex to be expressed in terms of a few parameters. A methodology previously designed by the author, based on acquisition of specific field data and subsequent processing by various Fourier techniques, enabled doline geometry to be considered in its entirety. This paper sets out to investigate which simple rotational body (rotated power function) best fits the geometry of 38 dolines studied within the Classical Karst of Slovenia. It transpires that doline radius to depth ratio changes as a function of volume, whereas the mode of change remains stable regardless of the actual dimensions of individual dolines, and is basically the same at different locations. Doline volumes are very close of those of regular cones of the same dimensions, growing relatively deeper as the volume increases. Two poly-segment models implied by the power function model and based on earlier work are discussed. It transpires that the studied dolines may be relict forms superimposed upon pre-existing hidden shafts formed by epikarstic water. Copyright © 2006 John Wiley & Sons, Ltd. [source] Heat transfer from a plate impinging swirl jetINTERNATIONAL JOURNAL OF ENERGY RESEARCH, Issue 4 2002Kadir Bilen Abstract Heat transfer and flow visualization experiments were conducted to investigate the performance of swirling and multi-channel impinging jets and compare the results with those of a multi-channel impinging jet (MCIJ) and conventional impinging jet (CIJ) for the present work at the same conditions. Swirling impinging jets (SIJs) employed the fixed blade lengths of 12.3 mm with four blades at the exit of the housing tube to divert the air flow through four narrow channels with a desired swirl angle (, of 22.5, 41 and 50°). The MCIJ jet had the same dimensions as the SIJs, except that the narrow channels in the solid insert were vertical (,=0°). The local and surface average Nusselt numbers of MCIJ were generally higher than those of the CIJ and SIJs. The SIJs, however, demonstrated significant improvement in radial uniformity of heat transfer compared to the MCIJ and CIJ. In the region of 2.7,X/D,0 for H/D=8 and Re=20 000, the average Nusselt number for the MCIJ was 11, 33, 72 and 98 per cent higher than that of the CIJ, ,=22.5, ,=41 and ,=50°, respectively. Copyright © 2002 John Wiley & Sons, Ltd. [source] Printed band-notched ultra-wideband quasi-dipole antennaMICROWAVE AND OPTICAL TECHNOLOGY LETTERS, Issue 3 2006Saou-Wen Su Abstract A printed ultra-wideband (UWB, 3.1,10.6 GHz) quasi-dipole antenna with the band-notched characteristic is presented. The antenna mainly comprises two radiating elements: an upper semi-circular disk and a lower semi-circular disk embedded with a pair of narrow slits, whose length is about one quarter-wavelength at the desired notched frequency. Both the upper and lower semi-circular disks are of the same dimensions, thus making the proposed antenna tend to be a dipole structure. With a compact size of 25 × 25 mm2, the proposed antenna showing UWB operation with a notched frequency band at 5 GHz is demonstrated. © 2006 Wiley Periodicals, Inc. Microwave Opt Technol Lett 48: 418,420, 2006; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.21368 [source] Self-Stigma Among Concealable Minorities in Hong Kong: Conceptualization and Unified MeasurementAMERICAN JOURNAL OF ORTHOPSYCHIATRY, Issue 2 2010Winnie W. S. Mak Self-stigma refers to the internalized stigma that individuals may have toward themselves as a result of their minority status. Not only can self-stigma dampen the mental health of individuals, it can deter them from seeking professional help lest disclosing their minority status lead to being shunned by service providers. No unified instrument has been developed to measure consistently self-stigma that could be applied to different concealable minority groups. The present study presented findings based on 4 studies on the development and validation of the Self-Stigma Scale, conducted in Hong Kong with community samples of mental health consumers, recent immigrants from Mainland China, and sexual minorities. Upon a series of validation procedures, a 9-item Self-Stigma Scale,Short Form was developed. Initial support on its reliability and construct validity (convergent and criterion validities) were found among 3 stigmatized groups. Utility of this unified measure was to establish an empirical basis upon which self-stigma of different concealable minority groups could be assessed under the same dimensions. Health-care professionals could make use of this short scale to assess potential self-stigmatization among concealable minorities, which may hamper their treatment process as well as their overall well-being. [source] | ||||||||||