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Automated Microscopy (automate + microscopy)

Distribution by Scientific Domains

Life Sciences	80%

Selected Abstracts

Use of automated microscopy for the detection of disseminated tumor cells in bone marrow samples

CYTOMETRY, Issue 4 2001
Elin Borgen
Abstract The use of automated microscopy has reached the maturity necessary for its routine use in the clinical pathology laboratory. In the following study we compared the performance of an automated microscope system (MDSÔ) with manual method for the detection and analysis of disseminated tumor cells present in bone marrow preparations from breast carcinoma patients. The MDS System detected rare disseminated tumor cells among bone marrow mononuclear cells with higher sensitivity than standard manual microscopy. Automated microscopy also proved to be a method of high reproducibility and precision, the advantage of which was clearly illustrated by problems of variability in manual screening. Accumulated results from two pathologists who had screened 120 clinical slides from breast cancer patients both by manual microscopy and by use of the MDS System revealed only two (3.8%) missed by the automatic procedure, whereas as many as 20 out of 52 positive samples (38%) were missed by manual screening. Cytometry (Comm. Clin. Cytometry) 46:215,221, 2001. © 2001 Wiley-Liss, Inc. [source]

Detailed examination of lymph nodes improves prognostication in colorectal cancer

INTERNATIONAL JOURNAL OF CANCER, Issue 11 2010
Fania S. Doekhie
Abstract Up to 30% of stage II patients with curatively resected colorectal cancer (CRC) will develop disease recurrence. We evaluated whether examination of lymph nodes by multilevel sectioning and immunohistochemical staining can improve prognostication. Lymph nodes (n = 780) from 36 CRC patients who had developed disease recurrence (cases) and 72 patients who showed no recurrence of disease for at least 5 years (controls) were analyzed. Sections of 4 levels at 200-,m interval were immunohistochemically stained for cytokeratin expression. The first level was analyzed by conventional and automated microscopy, and the 3 following levels were analyzed by automated microscopy for the presence of tumor cells. Overall, cases showed more micrometastases (3 patients) than controls (1 patient). Analysis of a second level led to the additional detection of 1 patient with micrometastases (case) and 1 patient with macrometastasis (case). Examining more levels only led to additional isolated tumor cells, which were equally divided between cases and controls. Likewise, automated microscopy resulted only in detection of additional isolated tumor cells when compared with conventional microscopy. In multivariate analysis, micrometastases [odds ratio (OR) 26.3, 95% confidence interval (CI) 1.9,364.8, p = 0.015], T4 stage (OR 4.8, 95% CI 1.4,16.7, p = 0.013) and number of lymph nodes (OR 0.9, 95% CI 0.8,1.0, p = 0.028) were independent predictors for disease recurrence. Lymph node analysis of 2 levels and immunohistochemical staining add to the detection of macrometastases and micrometastases in CRC. Micrometastases were found to be an independent predictor of disease recurrence. Isolated tumor cells were of no prognostic significance. [source]

Fetomaternal hemorrhage in relation to chorionic villus sampling revisited

PRENATAL DIAGNOSIS, Issue 3 2006
Denise M. Pelikan
Abstract Objective To investigate whether chorionic villus sampling (CVS) results in a proportional increase of alpha-fetoprotein (AFP) and fetal red cells in maternal blood. Methods Blood samples were collected before and after CVS. The AFP concentration was measured and supervised automated microscopy of Kleihauer,Betke slides was applied to quantify fetal red cells. Results AFP analysis was performed in 53 paired samples and automated microscopic scanning in 59 paired samples. Median AFP concentrations before and after CVS were 12.0 µg/L (range 6.4,36.4) and 18.7 µg/L (range 8.2,668.9), respectively, indicating a significant increase (p < 0.0001). Median numbers of fetal red cells detected before and after CVS were 0 (range 0,36) and 0 (range 0,31), respectively. No significant increase of fetal cells was observed (p = 0.72). The delta (,) fetal red cells and the , AFP correlated poorly (, = ,0.22, p = 0.11). The amount of villi correlated moderately with the , AFP (, = 0.32, p = 0.02) and did not correlate with the , fetal red cells (, = ,0.11, p = 0.43). Conclusions Although the AFP concentration after CVS increased, no increase of fetal red cells was detected. These findings suggest that CVS results in a leakage of proteins due to placental tissue damage, rather than increased trafficking of fetal cells. Copyright © 2006 John Wiley & Sons, Ltd. [source]