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Rosemary Leaves (rosemary + leaf)
Selected AbstractsCooperative antitumor effects of vitamin D3 derivatives and rosemary preparations in a mouse model of myeloid leukemiaINTERNATIONAL JOURNAL OF CANCER, Issue 12 2006Hagar Sharabani Abstract 1,,25-dihydroxyvitamin D3 (1,25D3) is a powerful differentiation agent, which has potential for treatment of myeloid leukemias and other types of cancer, but the calcemia produced by pharmacologically active doses precludes the use of this agent in the clinic. We have shown that carnosic acid, the major rosemary polyphenol, enhances the differentiating and antiproliferative effects of low concentrations of 1,25D3 in human myeloid leukemia cell lines (HL60, U937). Here we translated these findings to in vivo conditions using a syngeneic mouse leukemia tumor model. To this end, we first demonstrated that as in HL60 cells, differentiation of WEHI-3B D, murine myelomonocytic leukemia cells induced by 1 nM 1,25D3 or its low-calcemic analog, 1,25-dihydroxy-16-ene-5,6-trans-cholecalciferol (Ro25-4020), can be synergistically potentiated by carnosic acid (10 ,M) or the carnosic acid-rich ethanolic extract of rosemary leaves. This effect was accompanied by cell cycle arrest in G0+G1 phase and a marked inhibition of cell growth. In the in vivo studies, i.p. injections of 2 ,g Ro25-4020 in Balb/c mice bearing WEHI-3B D, tumors produced a significant delay in tumor appearance and reduction in tumor size, without significant toxicity. Another analog, 1,25-dihydroxy-16,23Z-diene-20-epi-26,27-hexafluoro-19-nor-cholecalciferol (Ro26-3884) administered at the same dose was less effective than Ro25-4020 and profoundly toxic. Importantly, combined treatment with 1% dry rosemary extract (mixed with food) and 1 ,g Ro25-4020 resulted in a strong cooperative antitumor effect, without inducing hypercalcemia. These results indicate for the first time that a plant polyphenolic preparation and a vitamin D derivative can cooperate not only in inducing leukemia cell differentiation in vitro, but also in the antileukemic activity in vivo. These data may suggest novel protocols for chemoprevention or differentiation therapy of myeloid leukemia. © 2006 Wiley-Liss, Inc. [source] Identification of Irradiated Spices Using the Novel Technique of DNA Comet AssayJOURNAL OF FOOD SCIENCE, Issue 2 2002A.A. Khan ABSTRACT: Microgel electrophoresis of single cells or nuclei (DNA comet assay) was investigated to identify irradiated spices. Ten spices treated with radiation doses in the range of 0 to 20 kGy were analyzed. After electrophoresis, radiation-damaged DNA appeared as a comet, whereas in non irradiated spices round or conical spots appeared. Shape, length, and intensity of comets were also dose-dependent. Detection was successful in poppy seeds, cardamom seeds, caraway seeds, and nigella seeds, but not in pomegranate seeds, ginger root, and juniper berries, where lysis was insufficient, and also not in black peppercorns, nutmeg seed, and rosemary leaves, where extraction of cells or nuclei failed. Nevertheless, for some irradiated foods the DNA comet assay is a rapid and inexpensive screening test. [source] IMPACT OF DRYING AND STORAGE TIME ON SENSORY CHARACTERISTICS OF ROSEMARY (ROSMARINUS OFFICINALIS L.)JOURNAL OF SENSORY STUDIES, Issue 1 2007M.C. DÍAZ-MAROTO ABSTRACT The effect of drying treatment on the aroma characteristics of rosemary (Rosmarinus Officinalis L.) was studied using the Quantitative Descriptive Analysis (QDA) with a trained panel and by Free Choice Profiling (FCP) analysis with a consumer group. Differences between samples were observed after applying Principal Component Analysis to the QDA data and Generalized Procrustes Analysis to the FCP data. The highest differences were found between fresh rosemary samples and dried samples. However, dried samples (obtained by oven-drying at 45C and commercial samples purchased at the markets and stored for 6 months) showed significant differences in their sensory characteristics. Odor detection thresholds of the fresh rosemary leaves were calculated in water and in potato sauce, and significant differences were found. [source] Supercritical CO2 Extraction of Essential Oil from Algerian Rosemary (Rosmarinus officinalis,L.)CHEMICAL ENGINEERING & TECHNOLOGY (CET), Issue 3 2010A. Zermane Abstract The present study presents experimental results concerning the supercritical CO2 extraction of essential oil from Algerian rosemary leaves. The effects of key operating parameters such as pressure, temperature, particle size and CO2 mass flow rate on the extraction yield were investigated. The obtained yields were in the range of 0.95,3.52,g oil/g dry rosemary, and the best value was observed at a pressure of 22,MPa, a temperature of 40,°C, a flow rate of 7,g/min, and a particle size of 1,mm. The performance of the local rosemary used was assessed by comparison of the obtained yields with values reported in the literature for essential oils derived from different rosemary sources. The GC and the GC-MS analyses showed that the major compound detected in the essential oil was camphor, at 48.89,wt,%. 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