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Remote Part (remote + part)
Selected AbstractsStructure determination of a Galectin-3,carbohydrate complex using paramagnetism-based NMR constraintsPROTEIN SCIENCE, Issue 7 2008Tiandi Zhuang Abstract The determination of the location and conformation of a natural ligand bound to a protein receptor is often a first step in the rational design of molecules that can modulate receptor function. NMR observables, including NOEs, often provide the basis for these determinations. However, when ligands are carbohydrates, interactions mediated by extensive hydrogen-bonding networks often reduce or eliminate NOEs between ligand and protein protons. In these cases, it is useful to look to other distance- and orientation-dependent observables that can constrain the geometry of ligand,protein complexes. Here we illustrate the use of paramagnetism-based NMR constraints, including pseudo-contact shifts (PCS) and field-induced residual dipolar couplings (RDCs). When a paramagnetic center can be attached to the protein, field-induced RDCs and PCS reflect only bound-state properties of the ligand, even when averages over small fractions of bound states and large fractions of free states are observed. The effects can also be observed over a long range, making it possible to attach a paramagnetic center to a remote part of the protein. The system studied here is a Galectin-3,lactose complex. A lanthanide-binding peptide showing minimal flexibility with respect to the protein was integrated into the C terminus of an expression construct for the Galectin-3,carbohydrate-binding domain. Dysprosium ion, which has a large magnetic susceptibility anisotropy, was complexed to the peptide, making it possible to observe both PCSs and field-induced RDCs for the protein and the ligand. The structure determined from these constraints shows agreement with a crystal structure of a Galectin-3,N -acetyllactosamine complex. [source] Decadal changes (1996,2006) in coastal ecosystems of the Chagos archipelago determined from rapid assessmentAQUATIC CONSERVATION: MARINE AND FRESHWATER ECOSYSTEMS, Issue 6 2009Andrew R.G. Price Abstract 1.The atolls and islands comprising Chagos are a biodiversity hotspot of global conservation significance in a remote part of the central Indian Ocean. 2.This study examines the condition of the archipelago's coastal ecosystems by rapid environmental assessment at 21 sites/islands, which were also investigated a decade earlier using the same methodology. Major changes in ecosystem structure and environmental disturbance were determined. 3.Coral fish abundance was significantly lower in 2006 than 1996. Decrease in the physical structural complexity of the reefs, as a result of coral bleaching and mortality induced by the 1998 warming event, may have been a contributing factor. 4.Evidence of collecting/fishing was significantly greater in 2006 than 1996. This is attributed mainly to an illegal fishery for holothurians (sea cucumbers), which has expanded over recent years and now exerts substantial pressure on the resource. The significant decline observed in beach wood, a readily accessible fuel for fishing camps, is consistent with this. 5.Solid waste on islands was high (median 2 to 20 items m,1 beach) in both 1996 and 2006. Potentially harmful biological impacts, determined from other studies, include entanglement, toxic effects and provision of transport for invasives or other ,hitchhiker' species. 6.Significantly higher bird abundances were recorded in protected areas than ,unprotected' areas, attributed mainly to absence of predation by rats. 7.Rapid assessment augments more comprehensive ecosystem investigations. It provides a valuable snapshot of environmental conditions based upon a broad suite of features (ecosystems and disturbances) determined, concurrently, within the same site inspection quadrats and using the same scale of assessment. Copyright © 2009 John Wiley & Sons, Ltd. [source] Farming flexibility and food security under climatic uncertainty: Manang, Nepal HimalayaAREA, Issue 2 2010Tor Halfdan Aase Climate change is predicted to cause a substantial decrease in food production in poor regions during this century. But since reliable models of future climates have not been produced at local level as yet, this article focuses on the flexibility of farming systems in terms of adaptability to changing conditions of production, whatever those changes may turn out to be. Defining flexibility as ,uncommitted potentiality for change'Bateson 1972, the aim of the article is to identify such potentialities among subsistence farmers in a remote part of the Himalayas. Our analysis reveals four ,uncommitted potentialities' for adaptation to a future situation that will be climatologically different from the present. In order to maintain local food security under changing climate conditions, farmers in the study valley of Manang can reclaim abandoned land, they can depend more on barley, they may reduce the conspicuous exhibition of horses, and relocate farming from the slope to the valley bottom. The inherent flexibility in their farming system renders Mananges quite robust in facing future uncertainties. Thus, Manang is more appropriately labelled ,dynamic' than ,fragile', which is a term often ascribed to high Himalayan communities and environments. [source] Effects of construction noise on behaviour of and exhibit use by Snow leopards Uncia uncia at Basel zooINTERNATIONAL ZOO YEARBOOK, Issue 1 2008C. E. SULSER Noise caused by human activities can cause stress in animals. We examined whether noise from construction sites affects the behaviour of and exhibit use by three Snow leopards Uncia uncia at Basel zoo. The behaviour and location of the animals were recorded at 1 minute intervals, using the instantaneous scan sampling method over a period of 216 hours (104 hours on noisy days and 112 hours on quiet days). The animals differed individually in their responses to the construction noise. On noisy days, the Snow leopards generally spent less time in locomotion and more time resting, but even on quiet days, resting was the predominant behaviour performed. Under noisy conditions, they increased social resting and decreased resting alone. Walking and social walking were also reduced on noisy days. Furthermore, the Snow leopards spent considerably more time in the remote off-exhibit enclosure under noisy conditions. Independent of background noise, they stayed more than half of the time in the caves and the forecourts of the outdoor enclosure. On quiet days, the Snow leopards used more sectors of their exhibit than on noisy days. The results indicate that the Snow leopards responded to construction noise by increasing the amount of time spent resting and by withdrawing to the remote parts of their exhibit. [source] Protocols, particularities, and problematising Indigenous ,engagement' in community-based environmental management in settled AustraliaTHE GEOGRAPHICAL JOURNAL, Issue 3 2010JENNIFER CARTER Many Aboriginal Australians in regional and urban Australia hold attachments to their homelands that have been compromised by policies of removal and dispossession. Government agencies and community groups have ,protocols' for engaging with Aboriginal communities, but these protocols have been transferred from remote parts of Australia where land tenure and rights are relatively secure and people can readily claim their community of belonging. The efficacy and applicability of engagement protocols are rarely evaluated, and have not been evaluated with respect to the differing tenure regimes of settled Australia under which rights to land and its resources remain contested and unfolding. This paper describes research conducted in three study areas of regional Australia, where resource management practitioners apply projects according to engagement protocols transferred from remote Australia. Analysis of government and community-based documents, and interviews with agency staff and Aboriginal people, identifies that genuine participation, cultural awareness, agreement-making, appropriate representation and the unique place-based factors affecting engagement remain key barriers to effective engagement with Aboriginal people by institutions in urbanising Australia. In particular, appropriate representation and a need for place-based approaches emerge as critical to engagement in settled Australia. This paper recommends that engagement be considered as a multi-layered approach in which generic ,engagement' threads are selected and re-selected in different combinations to suit contexts, places and purposes. Thus each place-based engagement initiative is not readily typified at the local scale, but taken together, make up a regional mosaic of different engagement structures and processes. [source] Betting on the evidence: Reported gambling problems among the Indigenous population of the Northern TerritoryAUSTRALIAN AND NEW ZEALAND JOURNAL OF PUBLIC HEALTH, Issue 6 2009Matthew Stevens Abstract Objectives: To address a shortfall in evidence with which to justify gambling-specific interventions for the Indigenous population, we analysed two surveys (2002 National Aboriginal and Torres Strait Islander Social Survey and General Social Survey) that contain information on reported gambling problems for the NT. Methods: Estimates of reported gambling problems are presented for each state and territory by remoteness for the Indigenous and total population for 2002. Factor analysis was used to identify the relationship between gambling problems and other negative life events for the NT Indigenous and total population. Results: High levels of reported gambling problems were apparent for the Indigenous population particularly in the remote parts of the NT and Queensland. Gambling problems were associated with other stressors relating to social transgressions. Among the NT Indigenous population, gambling problems were correlated with levels of crowding, community involvement, personal and community violence and self-assessed health status. Conclusions: The high levels of reported gambling problems suggest that gambling is causing significant problems for Indigenous people. The multivariable adjusted associations indicate that gambling-related problems are intimately connected to a range of community contexts. Implications: Policies of intervention need to address broader social and environmental contexts that are intrinsically associated with gambling (and associated problems), in addition to public education in harm associated with gambling and provision of counselling services to assist problem gamblers. [source] | |||||||||||||