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Rapid Assessment (rapid + assessment)
Selected AbstractsRapid assessment of ,-asarone content of Acorus calamus by micellar electrokinetic capillary chromatographyELECTROPHORESIS, Issue 4-5 2005Kim M. Hanson Abstract This report outlines a rapid, reproducible method for the determination of ,-asarone, a known carcinogen, using micellar electrokinetic capillary chromatography (MEKC)-UV-vis absorbance and a simple alcohol extraction. The MEKC method is based on a running buffer comprised of 100,mM sodium dodecyl sulfate (SDS), pH,10. The method is reproducible and provides baseline separation of ,-asarone and ,-asarone. This protocol was used to determine the ,-asarone content of Acorus calamus rhizome of a diploid variety harvested from the wetlands of the United States and the triploid variety from India obtained commercially. The results indicate raw product that originated from India contained 4.4%,w/w ,-asarone, while that from the United States contained 0.2%,w/w ,-asarone. Neither sample contained detectable concentrations of ,-asarone. This is the first report of the use of MEKC to determine asarone in a natural source. [source] Rapid assessment of in vivo cholinergic transmission by amperometric detection of changes in extracellular choline levelsEUROPEAN JOURNAL OF NEUROSCIENCE, Issue 6 2004Vinay Parikh Abstract Conventional microdialysis methods for measuring acetylcholine (ACh) efflux do not provide sufficient temporal resolution to relate cholinergic transmission to individual stimuli or behavioral responses, or sufficient spatial resolution to investigate heterogeneities in such regulation within a brain region. In an effort to overcome these constraints, we investigated a ceramic-based microelectrode array designed to measure amperometrically rapid changes in extracellular choline as a marker for cholinergic transmission in the frontoparietal cortex of anesthetized rats. These microelectrodes exhibited detection limits of 300 nm for choline and selectivity (> 100 : 1) of choline over interferents such as ascorbic acid. Intracortical pressure ejections of choline (20 mm, 66,400 nL) and ACh (10 and 100 mm, 200 nL) dose-dependently increased choline-related signals that were cleared to background levels within 10 s. ACh, but not choline-induced signals, were significantly attenuated by co-ejection of the acetylcholinesterase inhibitor neostigmine (Neo; 100 mm). Pressure ejections of drugs known to increase cortical ACh efflux, potassium (KCl; 70 mm, 66, 200 nL) and scopolamine (Scop; 10 mm, 200 nL), also markedly increased extracellular choline signals, which again were inhibited by Neo. Scop-induced choline signals were also found to be tetrodotoxin-sensitive. Collectively, these findings suggest that drug-induced increases in current measured with these microelectrode arrays reflect the oxidation of choline that is neuronally derived from the release and subsequent hydrolysis of ACh. Choline signals assessed using enzyme-selective microelectrode arrays may represent a rapid, sensitive and spatially discrete measure of cholinergic transmission. [source] Rapid assessment of the sex of codling moth Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae) eggs and larvaeJOURNAL OF APPLIED ENTOMOLOGY, Issue 4 2009I. Fuková Abstract Two different methods were tested to identify the sex of the early developmental stages of the codling moth Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae) with a WZ/ZZ (female/male) sex chromosome system. First, it was shown that the sex of all larval stages can be easily determined by the presence or absence of sex chromatin, which is formed by the female-specific W chromosome in interphase nuclei. This trait can also be used to identify the sex of newly hatched larvae but it does require care and accuracy. Secondly, a new sexing technique was developed based on a molecular marker of the codling moth W chromosome. Flanking regions of an earlier described W-specific sequence (CpW2) were isolated and sequenced and a 2.74 kb sequence (CpW2- EcoRI), specific for the W chromosome, was obtained. Several PCR tests were conducted, which confirmed that the CpW2- EcoRI sequence is a reliable marker for the sex identification in codling moth samples of different geographical origin. In addition, a fragment of a codling moth gene, period (Cpper) was isolated and sequenced. Results of southern hybridization of the Cpper probe with female and male genomic DNA suggested that the Cpper gene is located on the Z chromosome. Then a multiplex PCR assay was developed, which co-amplified the CpW2- EcoRI sequence to identify the W chromosome and the Z-linked Cpper sequence, which served as a positive control of accurate processing of tested samples. The multiplex PCR provides an easy and rapid identification of the sex of embryos and early larval instars of the codling moth. [source] Rapid assessment of a helpdesk service supporting severe acute respiratory syndrome patients and their relativesJOURNAL OF CLINICAL NURSING, Issue 6 2004Joanne WY Chung PhD Background., To contain severe acute respiratory syndrome, the Hong Kong Hospital Authority set a policy that stipulated there should be no visitors to hospital wards. A helpdesk service was established with the goal of providing immediate emotional and communication support to relatives while severe acute respiratory syndrome patients were isolated during the acute phase of the illness. Aim., This study describes the results of a rapid assessment of the effectiveness of a helpdesk service designed to meet the immediate needs of relatives of severe acute respiratory syndrome patients in Hong Kong. Design., Survey. Method., Eighty-three respondents, representing about 46.3% of relatives (179), attending the helpdesk on the day of the study were recruited. Service evaluation data was collected using a self-administered questionnaire completed by respondents. Results., Nearly 100% of respondents who used the service found the delivery service with on-site counselling useful for alleviating their anxiety. However, about half of these relatives complained of insufficient information regarding the patient's condition and progress. The majority of respondents were satisfied with the service. In describing the most important traits of the service providers, caring and enthusiasm were mentioned most frequently by respondents who stated that they were very satisfied with the service. Conclusion., The results support the value of the service, and demonstrate that the service is effective in meeting relatives' immediate needs. These needs include information, aid in fulfilling their role as caretaker for the patient (delivering prepared soup) and psychological support. The results suggest that facilitation of visitation of patients by relatives via video conferencing and education of the public on the nature and course of severe acute respiratory syndrome to reduce the social stigma of having a potentially life-threatening disease should be introduced in Hong Kong. Relevance to clinical practice., The results highlight important attributes that helpers (nurses) should have in order to alleviate the suffering of severe acute respiratory syndrome patients and their relatives. [source] Rapid assessment of internodal myelin integrity in central nervous system tissueJOURNAL OF NEUROSCIENCE RESEARCH, Issue 4 2010Daniel A. Kirschner Abstract Monitoring pathology/regeneration in experimental models of de-/remyelination requires an accurate measure not only of functional changes but also of the amount of myelin. We tested whether X-ray diffraction (XRD), which measures periodicity in unfixed myelin, can assess the structural integrity of myelin in fixed tissue. From laboratories involved in spinal cord injury research and in studying the aging primate brain, we solicited "blind" samples and used an electronic detector to record rapidly the diffraction patterns (30 min each pattern) from them. We assessed myelin integrity by measuring its periodicity and relative amount. Fixation of tissue itself introduced ±10% variation in periodicity and ±40% variation in relative amount of myelin. For samples having the most native-like periods, the relative amounts of myelin detected allowed distinctions to be made between normal and demyelinating segments, between motor and sensory tracts within the spinal cord, and between aged and young primate CNS. Different periodicities also allowed distinctions to be made between samples from spinal cord and nerve roots and between well-fixed and poorly fixed samples. Our findings suggest that, in addition to evaluating the effectiveness of different fixatives, XRD could also be used as a robust and rapid technique for quantitating the relative amount of myelin among spinal cords and other CNS tissue samples from experimental models of de- and remyelination. © 2009 Wiley-Liss, Inc. [source] Low-resolution remotely sensed images of winegrape vineyards map spatial variability in planimetric canopy area instead of leaf area indexAUSTRALIAN JOURNAL OF GRAPE AND WINE RESEARCH, Issue 1 2008A. HALL Abstract Background and Aims:, Knowledge of the spatial variability of grapevine canopy density is useful in managing the variability of grape composition and yield. Rapid assessment of the characteristics of vineyards by remote sensing offers distinct advantages over ground-based measurements. In an effort to capture such advantages, this study aimed to assess the relative contribution to LAI of grapevine canopy density and grapevine canopy area derived from high-spatial-resolution airborne digital imagery. Methods and Results:, High-spatial-resolution airborne NDVI imagery of minimally pruned, unconfined (i.e. not confined by trellising) grapevines was used to partition image pixels into grapevine-only and non-grapevine groupings. An evaluation of the relative contributions of grapevine planimetric area (number of grapevine pixels across a single row) and leaf layers (NDVI of grapevine-only pixels) found that the variability observed across the vineyard was dominated by changes in canopy area rather than grapevine-only NDVI. Conclusion:, The primary predictive variable of grapevine LAI is canopy area. Low-spatial-resolution NDVI imagery of minimally pruned, unconfined vineyards is therefore effective in mapping spatial variability in planimetric canopy area, rather than LAI. Significance of the Study:, The process of estimating grapevine LAI from mixed pixels has incorrectly assumed that both components of LAI within a pixel's footprint, namely the number of leaf layers and planimetric canopy area, produce a consistent response in NDVI. Correlations between NDVI and LAI reported in previous studies based on low-resolution imagery most likely relied on the proxy relationship between NDVI and canopy area. [source] Rapid assessment and safe management of severe pulmonary hypertension with milrinone during orthotopic liver transplantationCLINICAL TRANSPLANTATION, Issue 4 2010Kyota Fukazawa Fukazawa K, Poliac LC, Pretto EA. Rapid assessment and safe management of severe pulmonary hypertension with milrinone during orthotopic liver transplantation. Clin Transplant 2010: 24: 515,519. © 2009 John Wiley & Sons A/S. Abstract:, The incidence of porto-pulmonary hypertension (PPHN) in patients with end stage liver disease is 8.5%. Evidence indicates that proceeding with orthotopic liver transplantation (OLT) in patients diagnosed with severe PPHN (mean pulmonary artery pressure [mPAP] > 45 mmHg) at the time of OLT surgery is associated with high perioperative mortality. We describe a case of severe PPHN that was diagnosed by right heart catheterization at the time of surgery. We quickly determined the reversibility of PPHN with a bolus of milrinone and proceeded with OLT. Further episodes of pulmonary hypertension were successfully managed with continuous milrinone infusion and transesophageal echocardiography monitoring. Reversibility via vasodilator trial after identification of high pulmonary artery pressures (PAP) may be an important indication of the feasibility of OLT. Milrinone may be useful for the rapid identification of the reversibility of high PAP and may be an effective agent to control abrupt increases in PAP during OLT. [source] Rapid humanitarian assessments and rationality: a value-of-information study from Iraq, 2003-04DISASTERS, Issue 1 2007Aldo Benini Rapid assessments are one of the standard informational tools in humanitarian response and are supposed to contribute to rational decision-making.1 The extent to which the assessment organisation itself behaves rationally, however, is an open question. This can be evaluated against multiple criteria, such as the cost and value of the information it collects and its ability to adapt flexibly design or samples when the survey environment changes unforeseeably. An unusual data constellation from two concurrent recent (2003,04) rapid assessments in northern Iraq permits us to model part of the actual assessment behaviour in terms of geographical, community and prior substantive information attributes. The model correctly predicts the decisions, in 79 per cent of the 2,425 local communities in focus, that data collector teams in the Emergency Mine Action Survey made to visit or not to visit. The analysis demonstrates variably rational behaviour under conditions of insecurity, repeated regrouping and incomplete sampling frames. A pronounced bias towards very small rural settlements is irrational for the overall results, but may be a rational strategy of individual survey workers seeking to prolong their employment. Implications for future assessments are sketched in the areas of tools for urban surveys, greater adaptability, including early feedback from users, and sensibility to value-of-information concepts. [source] Planning Environmental Sanitation Programmes in EmergenciesDISASTERS, Issue 2 2005Peter A. Harvey Environmental sanitation programmes are vital for tackling environmental-related disease and ensuring human dignity in emergency situations. If they are to have maximum impact they must be planned in a rapid but systematic manner. An appropriate planning process comprises five key stages: rapid assessment and priority setting; outline programme design; immediate action; detailed programme design; and implementation. The assessment should be based on carefully selected data, which are analysed via comparison with suitable minimum objectives. How the intervention should be prioritised is determined through objective ranking of different environmental sanitation sector needs. Next, a programme design outline is produced to identify immediate and longer-term intervention activities and to guarantee that apposite resources are made available. Immediate action is taken to meet acute emergency needs while the detailed programme design takes shape. This entails in-depth consultation with the affected community and comprehensive planning of activities and resource requirements. Implementation can then begin, which should involve pertinent management and monitoring strategies. [source] The Application of Geographic Information Systems and Global Positioning Systems in Humanitarian Emergencies: Lessons Learned, Programme Implications and Future ResearchDISASTERS, Issue 2 2003Reinhard Kaiser Geographic information systems (GIS), global positioning systems and remote sensing have been increasingly used in public health settings since the 1990s, but application of these methods in humanitarian emergencies has been less documented. Recent areas of application of GIS methods in humanitarian emergencies include hazard, vulnerability, and risk assessments; rapid assessment and survey methods; disease distribution and outbreak investigations; planning and implementation of health information systems; data and programme integration; and programme monitoring and evaluation. The main use of GIS in these areas is to provide maps for decision-making and advocacy, which allow overlaying types of information that may not normally be linked. GIS is also used to improve data collection in the field (for example, for rapid health assessments or mortality surveys). Development of GIS methods requires further research. Although GIS methods may save resources and reduce error, initial investment in equipment and capacity building may be substantial. Especially in humanitarian emergencies, equipment and methodologies must be practical and appropriate for field use. Add-on software to process GIS data needs to be developed and modified. As equipment becomes more user-friendly and costs decrease, GIS will become more of a routine tool for humanitarian aid organisations in humanitarian emergencies, and new and innovative uses will evolve. [source] The Use of Cluster Sampling to Determine Aid Needs in Grozny, Chechnya in 1995DISASTERS, Issue 3 2000Sean Drysdale War broke out in Chechnya in November 1994 following a three-year economic blockade. It caused widespread destruction in the capital Grozny. In April 1995 Medical Relief International - or Merlin, a British medical non-governmental organisation (NGO) - began a programme to provide medical supplies, support health centres, control communicable disease and promote preventive health-care in Grozny. In July 1995 the agency undertook a city-wide needs assessment using a modification of the cluster sampling technique developed by the Expanded Programme on Immunisation. This showed that most people had enough drinking-water, food and fuel but that provision of medical care was inadequate. The survey allowed Merlin to redirect resources earmarked for a clean water programme towards health education and improving primary health-care services. It also showed that rapid assessment by a statistically satisfactory method is both possible and useful in such a situation. [source] Review of dyspnoea quantification in the emergency department: Is a rating scale for breathlessness suitable for use as an admission prediction tool?EMERGENCY MEDICINE AUSTRALASIA, Issue 5 2007Amanda Saracino Abstract Acute shortness of breath is a potential marker of serious cardiopulmonary disease and requires rapid assessment. In our current health-care system, increasing pressure on the ED to limit costs and waiting times has resulted in the development of many clinical decision aids and admission prediction tools designed to assist ED physicians in meeting these demands. However, most of these tools are disease specific, and none are currently available for application to patients presenting to the ED with shortness of breath. Although somewhat limited, current evidence supports the utilization of a simple dyspnoea rating scale, to assist in the streamlining of clinical severity assessments and urgency evaluations, and to potentially provide useful information to facilitate rapid and accurate site-of-care decisions in this setting. [source] Metal toxicity inferred from algal population density, heterotrophic substrate use, and fatty acid profile in a small streamENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 4 2000Robert B. Genter Abstract The purpose of this study was to examine relations between metal concentrations in periphyton and the abundance of algal species, heterotrophic use of 95 carbon sources, and phospholipid fatty acids (PLFA) of the periphyton in a small stream spanning a mine in Lemhi County, Idaho, USA. Two upstream, two mine, and two downstream sites were examined. Elevated concentrations of As and Cu at the mine sites were associated with communities that were depleted of diatoms and filamentous blue-green algae and characterized by a low-diversity community dominated by a single blue-green alga and patchy populations of the diatom Achnanthidium minutissimum and a filamentous green alga. Carbon source use and PLFA profiles provided a rapid assessment of stream conditions that were consistent with algal taxonomy and with our hypotheses constructed from previous reports on periphyton responses to metal stress. [source] A Smart Nanoprobe Based On Fluorescence-Quenching PEGylated Nanogels Containing Gold Nanoparticles for Monitoring the Response to Cancer TherapyADVANCED FUNCTIONAL MATERIALS, Issue 6 2009Motoi Oishi Abstract A biocompatible, caspase-3-responsive, and fluorescence-quenching smart apoptosis nanoprobe based on a PEGylated nanogel that contains gold nanoparticles (GNPs) (fluorescence quenchers) in the cross-linked polyamine gel core and fluorescein isothiocyanate (FITC)-labeled DEVD peptides at the tethered PEG chain ends is prepared for monitoring the cancer response to therapy. FITC,DEVD,nanogel,GNP shows very little fluorescence in the absence of activated caspase-3 (normal cells) through the fluorescence resonance energy transfer (FRET) process between the GNPs and the FITC molecules, while pronounced fluorescence signals are observed in apoptotic cells because of the cleavage of the DEVD peptide by activated caspase-3 present in the cells, which results in the release of FITC molecules. Thus, remarkable quenching and dequenching of fluorescence signals in response to activated caspase-3 is observed. Apoptotic cells are detected in human hepatocyte (HuH-7) multicellular tumor spheroids (MCTSs), a commonly used three-dimensional in vitro model mimicking the in vivo biology of tumors, as early as one day post-treatment with staurosporine, an apoptosis-inducing agent; while growth inhibition (i.e., change in size) of the HuH-7 MCTSs is only observed after a delay of three days (i.e., on day 4). This demonstrates the effectiveness of the FITC,DEVD,nanogel,GNP probe as a smart nanoprobe for real-time monitoring as well as a more rapid assessment of the early response to cancer therapy. [source] Pyrosequencing for detection of mutations in the connexin 26 (GJB2) and mitochondrial 12S RNA (MTRNR1) genes associated with hereditary hearing loss,HUMAN MUTATION, Issue 4 2002Alessandro Ferraris Abstract Hereditary hearing loss (HHL) is one of the most common congenital disorders and is highly heterogeneous. Mutations in the connexin 26 (CX26) gene (GJB2) account for about 20% of all cases of childhood deafness, and approach 50% in documented recessive cases of non-syndromic hearing loss. In addition, a single mitochondrial DNA mutation, mt1555A>G, in the 12S rRNA gene (MTRNR1), is associated with familial cases of progressive deafness. Effective screening of populations for HHL necessitates rapid assessment of several of these potential mutation sites. Pyrosequencing links a DNA synthesis protocol for determining sequence to an enzyme cascade that generates light whenever pyrophosphate is released during primer strand elongation. We assessed the ability of Pyrosequencing to detect common mutations causing HHL. Detection of the most common CX26 mutations in individuals of Caucasian (35delG), Ashkenazi (167delT), and Asian (235delC, V37I) descent was confirmed by Pyrosequencing. A total of 41 different mutations in the CX26 gene and the mitochondrial mt1555A>G mutation were confirmed. Genotyping of up to six different adjacent mutations was achieved, including simultaneous detection of 35delG and 167delT. Accurate and reproducible results were achieved taking advantage of assay flexibility and experimental conditions easily optimized for a high degree of standardization and cost-effectiveness. The standardized sample preparation steps, including target amplification by PCR and preparation of single-stranded template combined with automated sequence reaction and automated genotype scoring, positions this approach as a potentially high throughput platform for SNP/mutation genotyping in a clinical laboratory setting. Hum Mutat 20:312,320, 2002. © 2002 Wiley-Liss, Inc. [source] Directions of preferential flow in a hillslope soil, 1.HYDROLOGICAL PROCESSES, Issue 4 2005Quasi-steady flow Abstract Preferred infiltration is mainly perceived as vertically down whereas subsurface storm flow is thought to occur parallel to slopes. The transition from vertical to lateral flow in a layered hillslope soil is the focus of the contribution. Transient flow is assumed to move as a wetting front. Three time-domain reflectometry (TDR) wave-guides, each 0·15 m long, were mounted in the shape of a truncated tetrahedron with its peak pointing down. Each wave-guide focuses the front velocity along its axis. The three front-velocity vectors are decomposed into their x, y and z components, which are then assembled to the resultant velocity vector. The volume density flux of preferred flow is the product of the front velocity and the mobile water content. The latter is the amplitude of transient soil moisture measured with each wave-guide. The resultant vector of the volume flux density is computed similarly to the velocity vector. The experimental approach allows for the rapid assessment of transient flows without relying on the variation of water potentials. The experiments indicate that the directions of the resultant vectors of velocity and volume flux density can be estimated if the moisture variations of the three TDR wave-guides are strongly correlated during the passing of the wetting front. Copyright © 2004 John Wiley & Sons, Ltd. [source] In vivo analysis of gut function and disease changes in a zebrafish larvae model of inflammatory bowel disease: A feasibility studyINFLAMMATORY BOWEL DISEASES, Issue 7 2010Angeleen Fleming PhD Abstract Background: The aim of this study was to develop a model of inflammatory bowel disease (IBD) in zebrafish larvae, together with a method for the rapid assessment of gut morphology and function in vivo thereby enabling medium-throughput compound screening. Methods: Assays were performed using larval zebrafish from 3,8 days postfertilization (d.p.f.) in 96-well plates. Gut morphology and peristalsis were observed in vivo using fluorescent imaging following ingestion of fluorescent dyes. IBD was induced by addition of 2,4,6-trinitrobenzenesulfonic acid (TNBS) to the medium within the well. Pathology was assessed in vivo using fluorescent imaging and postmortem by histology, immunohistochemistry, and electron microscopy. Therapeutic compounds were evaluated by coadministration with TNBS. Results: A novel method of investigating gut architecture and peristalsis was devised using fluorescent imaging of live zebrafish larvae. Archetypal changes in gut architecture consistent with colitis were observed throughout the gut. Significant changes in goblet cell number and tumor necrosis factor alpha (TNF-,) antibody staining were used to quantify disease severity and rescue. Prednisolone and 5-amino salicylic acid treatment ameliorated the disease changes. Candidate therapeutic compounds (NOS inhibitors, thalidomide, and parthenolide) were assessed and a dissociation was observed between efficacy assessed using a single biochemical measure (TNF-, staining) versus an assessment of the entire disease state. Conclusions: Gut physiology and pathology relevant to human disease state can be rapidly modeled in zebrafish larvae. The model is suitable for medium-throughput chemical screens and is amenable to genetic manipulation, hence offers a powerful novel premammalian adjunct to the study of gastrointestinal disease. (Inflamm Bowel Dis 2010) [source] Evaluation of Ssp I polymerase chain reaction assay in the detection of Wuchereria bancrofti infection in field-collected Culex quinquefasciatus and its application in the transmission studies of lymphatic filariasisJOURNAL OF APPLIED ENTOMOLOGY, Issue 7-8 2002S. L. Hoti The evaluation of the assay was carried out using pools of vector mosquitoes collected from areas under filariasis survey and control trial projects, in comparison with the standard dissection and microscopy technique. In the filariasis survey area the infection rate as determined by the dissection and microscopy technique was 0.89% whereas it was 1.7% by PCR assay. In the Bacillus sphaericus trial area the infection rates as assessed by the conventional technique were 6.6 and 4.5% in the treated and check areas, respectively, whereas those obtained by the PCR assay were 4.7 to 2.2%. Although the infection rates determined by the PCR assay are slightly higher or lower than the rates obtained by the conventional technique, the difference was not statistically significant (P=0.451 for filariasis survey area, and P=0.203 and 0.161 for B. sphaericus trial area). When the pool size of Cx. quinquefasciatus was increased to 50 the sensitivity of the PCR was affected. The changes in infection rates as influenced by the antifilarial chemotherapy were similar when determined by PCR assay and the standard method. The major advantage of the PCR assay over the conventional technique is that thousands of mosquitoes can be processed within a short duration and this attribute has potential application in rapid assessment of disease prevalence and monitoring of the transmission dynamics. [source] Rapid assessment of a helpdesk service supporting severe acute respiratory syndrome patients and their relativesJOURNAL OF CLINICAL NURSING, Issue 6 2004Joanne WY Chung PhD Background., To contain severe acute respiratory syndrome, the Hong Kong Hospital Authority set a policy that stipulated there should be no visitors to hospital wards. A helpdesk service was established with the goal of providing immediate emotional and communication support to relatives while severe acute respiratory syndrome patients were isolated during the acute phase of the illness. Aim., This study describes the results of a rapid assessment of the effectiveness of a helpdesk service designed to meet the immediate needs of relatives of severe acute respiratory syndrome patients in Hong Kong. Design., Survey. Method., Eighty-three respondents, representing about 46.3% of relatives (179), attending the helpdesk on the day of the study were recruited. Service evaluation data was collected using a self-administered questionnaire completed by respondents. Results., Nearly 100% of respondents who used the service found the delivery service with on-site counselling useful for alleviating their anxiety. However, about half of these relatives complained of insufficient information regarding the patient's condition and progress. The majority of respondents were satisfied with the service. In describing the most important traits of the service providers, caring and enthusiasm were mentioned most frequently by respondents who stated that they were very satisfied with the service. Conclusion., The results support the value of the service, and demonstrate that the service is effective in meeting relatives' immediate needs. These needs include information, aid in fulfilling their role as caretaker for the patient (delivering prepared soup) and psychological support. The results suggest that facilitation of visitation of patients by relatives via video conferencing and education of the public on the nature and course of severe acute respiratory syndrome to reduce the social stigma of having a potentially life-threatening disease should be introduced in Hong Kong. Relevance to clinical practice., The results highlight important attributes that helpers (nurses) should have in order to alleviate the suffering of severe acute respiratory syndrome patients and their relatives. [source] Bridging the gap between evidence and practice in acute decompensated heart failure managementJOURNAL OF HOSPITAL MEDICINE, Issue S6 2008FACP, Franklin A. Michota Jr MD Abstract Registry data indicate a gap between evidence-based guidelines and current management of patients with acute decompensated heart failure (ADHF). Bridging this gap is crucial given the frequency and cost of hospitalization for this disorder. Patients with ADHF require rapid assessment to determine appropriate treatment location and initial therapy. Patients with impending respiratory failure or cardiogenic shock should be managed in an intensive care setting, patients with congestion that is expected to require prolonged intravenous therapy should be admitted to the hospital, and patients with congestion that is likely to respond within 12,24 hours can be managed in an observation unit. Clinical status should guide selection of initial therapy. Initially, therapeutic response should be assessed every couple of hours. Once effective acute therapy has been established, it is important to implement strategies to improve long-term outcomes. These strategies include ensuring that care complies with established core performance measures, providing patient education in a manner suited to ensure comprehension and retention, and arranging for appropriate outpatient follow-up, ideally in a comprehensive heart failure disease management program. The purpose of this review is (1) to examine evidence-based guidelines for the treatment of ADHF, (2) to present a practical algorithm for patient assessment and treatment derived from these guidelines and personal experience, and (3) to discuss systems to enhance the ultimate transition of patient care from the inpatient to outpatient setting. Journal of Hospital Medicine 2008;3(Suppl 6):S7,S15. ©2008 Society of Hospital Medicine. [source] N-in-1 dosing pharmacokinetics in drug discovery: Experience, theoretical and practical considerationsJOURNAL OF PHARMACEUTICAL SCIENCES, Issue 7 2008Kan He Abstract N-in-1 (or cassette) dosing pharmacokinetics (PK) has been used in drug discovery for rapid assessment of PK properties of new chemical entities. However, because of potential for drug,drug interactions this procedure is still controversial. This study was to retrospectively evaluate the N-in-1 dosing approach in drug discovery with an emphasis on the potential for drug,drug interactions. The systemic clearance, volume of distribution, oral bioavailability, and renal excretion of the 31 lead compounds in rats, dogs or chimpanzees were significantly correlated between the N-in-1 dosing and discrete studies with r values of 0.69, 0.91, 0.53, and 0.83 (p,<,0.005 for all), respectively. PK parameters for 11 quality control compounds which were involved in 194 N-in-1 studies for screening approximately 1000 compounds had coefficient of variations of less than 70%. The intrinsic microsomal clearances generated from the N-in-1 and discrete incubations were nearly identical (r,=,0.97, p,<,0.0001). The intrinsic clearances of quality control compound from the N-in-1 incubations were consistent with its discrete CLint estimate (cv: 5.4%). Therefore, N-in-1 dosing is a useful approach in drug discovery to quickly obtain initial PK estimates. Potential drug,drug interactions that result in confounding PK estimates do not occur as frequently as expected. © 2007 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 97:2568,2580, 2008 [source] Positive end-expiratory pressure optimization using electric impedance tomography in morbidly obese patients during laparoscopic gastric bypass surgeryACTA ANAESTHESIOLOGICA SCANDINAVICA, Issue 7 2006K. Erlandsson Background:, Morbidly obese patients have an increased risk for peri-operative lung complications and develop a decrease in functional residual capacity (FRC). Electric impedance tomography (EIT) can be used for continuous, fast-response measurement of lung volume changes. This method was used to optimize positive end-expiratory pressure (PEEP) to maintain FRC. Methods:, Fifteen patients with a body mass index of 49 ± 8 kg/m2 were studied during anaesthesia for laparoscopic gastric bypass surgery. Before induction, 16 electrodes were placed around the thorax to monitor ventilation-induced impedance changes. Calibration of the electric impedance tomograph against lung volume changes was made by increasing the tidal volume in steps of 200 ml. PEEP was titrated stepwise to maintain a horizontal baseline of the EIT curve, corresponding to a stable FRC. Absolute FRC was measured with a nitrogen wash-out/wash-in technique. Cardiac output was measured with an oesophageal Doppler method. Volume expanders, 1 ± 0.5 l, were given to prevent PEEP-induced haemodynamic impairment. Results:, Impedance changes closely followed tidal volume changes (R2 > 0.95). The optimal PEEP level was 15 ± 1 cmH2O, and FRC at this PEEP level was 1706 ± 447 ml before and 2210 ± 540 ml after surgery (P < 0.01). The cardiac index increased significantly from 2.6 ± 0.5 before to 3.1 ± 0.8 l/min/m2 after surgery, and the alveolar dead space decreased. PaO2/FiO2, shunt and compliance remained unchanged. Conclusion:, EIT enables rapid assessment of lung volume changes in morbidly obese patients, and optimization of PEEP. High PEEP levels need to be used to maintain a normal FRC and to minimize shunt. Volume loading prevents circulatory depression in spite of a high PEEP level. [source] A chromametric method for the rapid assessment of deep frying oil qualityJOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 13 2003Xin-Qing Xu Abstract A rapid chromametric method was developed for the assessment of deep frying oil quality based on the strong correlation between colour index and total polar compounds in deep frying oil. Colour indices of frying oil samples, measured by chromameter, decreased significantly during frying and were strongly correlated with frying time (r , 0.95, p < 0.001). Colour indices of a set of oil samples taken from 0 to 80 h of deep frying were also significantly correlated with total polar compounds of the same samples determined using the official method of the American Oil Chemists' Society (r = 0.96, p < 0.001). The equation for conversion of the colour index (x) to the content of total polar compounds (y) in an oil sample is y = 0.0174x2 , 2.9506x + 124.34. In addition, colour indices of 10 different types of frying oils were strongly correlated with the corresponding contents of total polar compounds in the oils with samples taken from 0 to 80 h of deep frying in duplicate (r = 0.95, p < 0.001, n = 220). The results of colour index analyses agreed well with the results of chemical and sensory analyses of the frying oils tested. This chromametric method is rapid, convenient and reliable. Copyright © 2003 Society of Chemical Industry [source] GIS-based rapid assessment of erosion risk in a small catchment in the wet/dry tropics of AustraliaLAND DEGRADATION AND DEVELOPMENT, Issue 5 2001G. Boggs Abstract Assessing the impact of various land uses on catchment erosion processes commonly requires in-depth research, monitoring and field data collection, as well as the implementation of sophisticated modelling techniques. This paper describes the evaluation of a geographic information system (GIS)-based rapid erosion assessment method, which allows the user to quickly acquire and evaluate existing data to assist in the planning of more detailed monitoring and modelling programmes. The rapid erosion assessment method is based on a simplified version of the revised universal soil loss equation (RUSLE), and allows the rapid parameterization of the model from widely available land unit and elevation datasets. The rapid erosion assessment method is evaluated through the investigation of the effects of elevation data resolution on erosion predictions and field data validation. The use of raster digital elevation model (DEM)-derived data, as opposed to vector land unit relief data, was found to greatly improve the validity of the rapid erosion assessment method. Field validation of the approach, involving the comparison of predicted soil loss ratios with adjusted in-stream sediment yields on a subcatchment basis, indicated that with decreasing data resolution, the results are increasingly overestimated for larger catchments and underestimated for smaller catchments. However, the rapid erosion assessment method proved to be a valuable tool that is highly useful as an initial step in the planning of more detailed erosion assessments. Copyright © 2001 Commonwealth of Australia. [source] Detecting the effects of introduced species: a case study of competition between Apis and BombusOIKOS, Issue 3 2006Diane M. Thomson Developing tools for rapid assessment of introduced species impacts is one of the most important challenges in invasion ecology. Most assessments of impact rely on correlational data or other indirect measures. Yet few studies have evaluated invasion effects using multiple, simultaneously applied monitoring and experimental approaches, in order to compare easily obtained metrics with more difficult but direct measures of reproductive success or population dynamics. In this study, I use data from an experimental test of introduced honey bee (Apis mellifera) impacts on native bumble bees (Bombus spp.) to address two major questions: 1) how well did observational data on niche overlap and spatial correlations between Apis and Bombus predict the results of experimental tests of competitive effects? and 2) how well did effects of the experimental Apis manipulations on Bombus foragers, which are easy to observe, predict changes in reproductive success of colonies, which are difficult to measure? Niche overlap between Apis and Bombus varied substantially, but increased to levels as high as 80,90% during periods of resource scarcity. Correlations between numbers of Apis foragers and numbers of Bombus foragers were also highly variable, but I detected a significant negative relationship in only one of the seven months observed. In contrast, the experimental results showed that mean numbers of Bombus foragers observed on a given transect increased significantly with greater distance from introduced Apis colonies. Of these three measures (niche overlap, correlations in abundances, and effects of experimental introductions), only the experimental data on forager abundances accurately estimated competitive effects on colony reproductive success previously reported for the same experiment, and the correlational data in particular completely failed to predict the effects observed in the experimental study. This work suggests that great caution is warranted in making assessments of invasion impact on the basis of spatial or temporal correlations between invasive and native species. Thus, investing in even small and limited experimental studies may be more valuable than extensive observational work in quantifying invasion impacts. [source] Rapid prenatal diagnosis of aneuploidies and zygosity in multiple pregnancies by amniocentesis with single insertion of the needle and quantitative fluorescent PCRPRENATAL DIAGNOSIS, Issue 8 2003Vincenzo Cirigliano Abstract Objective To investigate amniotic fluid (AF) samples retrieved in multiple pregnancies by single insertion of the needle, for rapid assessment of chromosome copy number, zygosity, and cross-contamination between fetuses, using Quantitative Fluorescent Polymerase Chain Reaction (QF-PCR) amplification of highly polymorphic microsatellite markers. Methods Fifty-two multiple pregnancies were selected (47 twins, 5 triplets) and 108 samples of amniotic fluid were sampled between 12 to 20 weeks of gestation (mean 15.5) using the single-needle technique. Aneuploidy screening by QF-PCR amplification of short tandem repeats (STRs) on chromosomes X, Y, 21, 13, and 18 was carried out within 24 h of collection. Owing to the sampling procedure, the eventual presence of contamination between fetuses was also evaluated in every case. Results Normal and aneuploid fetuses were readily identified by QF-PCR. Fetal reduction was made available, for trisomic fetuses, without further waiting for completion of fetal karyotyping. In twin gestations, the ultrasound examination of chorionicity was always in agreement with the molecular assessment of zygosity. Contamination between fetuses due to the sampling procedure with a single puncture was never observed. Conclusion Rapid prenatal diagnosis of aneuploidies by QF-PCR is a sensitive, efficient, and reliable assay. When applied in multiple pregnancies, it has the added value of allowing the assessment of zygosity in all cases, independently of chorionicity and fetal sex. Copyright © 2003 John Wiley & Sons, Ltd. [source] Point-of-care testingANAESTHESIA, Issue 8 2001apparatus Point-of-care testing of coagulation parameters provides a more rapid assessment of test results compared with laboratory testing. A new coagulation monitor (GEM® PCL, Instrumentation Laboratory, Kirchheim, Germany) was evaluated. Point-of-care data for activated partial thromboplastin time and prothrombin time (expressed as the international normalised ratio) and turn-around-time were compared. Coagulation parameters were compared in the blood of 57 patients with and without heparin therapy. The point-of-care and laboratory test results showed a bias (SD) of ,,0.26 (4.55) s for activated partial thromboplastin time and ,,0.011 (0.150) s for prothrombin time. The average turn-around-time was 3 min for point-of-care testing vs. 52 min for laboratory testing. We conclude that the reliability of point-of-care testing is sufficient for clinical use. [source] Effect of unionized ammonia, viscosity and protozoan contamination on the enzyme activity of the rotifer Brachionus plicatilisAQUACULTURE RESEARCH, Issue 4 2000A B. De Araujo Substrates that are cleaved to yield fluorescent products can be used to quickly quantify enzyme activity in vivo with image analysis or in vitro with fluorometry. This study was carried out to determine whether enzyme activity in rotifers is useful for assessing the physiological condition of rotifers. Neonates of Brachionus plicatilis Müller hatched from cysts were exposed to a concentration series of unionized ammonia ranging from 0 to 9.8 p.p.m., increasing seawater viscosity relative to 1.17 to control sea water by the addition of methyl cellulose and the addition of the protozoan Euplotes sp. to a density of 40 mL,1. Rotifer glucosidase and esterase activities decreased with increasing unionized ammonia and viscosity respectively. Activities of glucosidase and phospholipase decreased with increasing protozoan contamination. There was a significant relationship between enzyme activities and rotifer population growth. In vivo activities of certain rotifer enzymes can therefore serve as biomarkers for the rapid assessment of environmental stressors in rotifer mass cultures. [source] Have we neglected the societal importance of sand dunes?AQUATIC CONSERVATION: MARINE AND FRESHWATER ECOSYSTEMS, Issue 4 2010An ecosystem services perspective Abstract 1.Coastal sand dunes are widespread worldwide, including around the coasts of the British Isles and Europe, providing a wide range of functions some of which are recognized for their socio-economic benefits. 2.In some localities, their contribution to coastal defence and to tourism and regional character have been acknowledged in local plans, but this is far from ubiquitous. 3.A rapid assessment was undertaken of the range of ecosystem services provided by coastal sand dune systems, using the Millennium Ecosystem Assessment ecosystem services classification augmented with habitat- and locally-appropriate additions. 4.Sand dunes were shown to provide a wide range of provisioning, regulatory, cultural and supporting services, many of which remain substantially overlooked. 5.Although the importance of coastal sand dune for a diversity of characteristic and often rare organisms from a variety of taxa has been addressed, many of the broader ecosystem services that these habitats provide to society have been overlooked. This suggests that coastal sand dune systems are neglected ecosystems of significant and often under-appreciated societal value. Copyright © 2010 John Wiley & Sons, Ltd. [source] Decadal changes (1996,2006) in coastal ecosystems of the Chagos archipelago determined from rapid assessmentAQUATIC CONSERVATION: MARINE AND FRESHWATER ECOSYSTEMS, Issue 6 2009Andrew R.G. Price Abstract 1.The atolls and islands comprising Chagos are a biodiversity hotspot of global conservation significance in a remote part of the central Indian Ocean. 2.This study examines the condition of the archipelago's coastal ecosystems by rapid environmental assessment at 21 sites/islands, which were also investigated a decade earlier using the same methodology. Major changes in ecosystem structure and environmental disturbance were determined. 3.Coral fish abundance was significantly lower in 2006 than 1996. Decrease in the physical structural complexity of the reefs, as a result of coral bleaching and mortality induced by the 1998 warming event, may have been a contributing factor. 4.Evidence of collecting/fishing was significantly greater in 2006 than 1996. This is attributed mainly to an illegal fishery for holothurians (sea cucumbers), which has expanded over recent years and now exerts substantial pressure on the resource. The significant decline observed in beach wood, a readily accessible fuel for fishing camps, is consistent with this. 5.Solid waste on islands was high (median 2 to 20 items m,1 beach) in both 1996 and 2006. Potentially harmful biological impacts, determined from other studies, include entanglement, toxic effects and provision of transport for invasives or other ,hitchhiker' species. 6.Significantly higher bird abundances were recorded in protected areas than ,unprotected' areas, attributed mainly to absence of predation by rats. 7.Rapid assessment augments more comprehensive ecosystem investigations. It provides a valuable snapshot of environmental conditions based upon a broad suite of features (ecosystems and disturbances) determined, concurrently, within the same site inspection quadrats and using the same scale of assessment. Copyright © 2009 John Wiley & Sons, Ltd. [source] | |||||||||||||||||||||||||||||||