Home  About us  Contact
 

Putative Parents (putative + parent)

Distribution by Scientific Domains
Life Sciences100%

Selected Abstracts

Towards natural polyploid model organisms

MOLECULAR ECOLOGY, Issue 8 2008
RICHARD J. A. BUGGS
Abstract Populations of natural allopolyploids with available and well-developed genomic resources are currently hard to come by. These are needed because whole genome duplication and hybridization , both combined in allopolyploids , are significant processes in evolution, especially the evolution of plants. The new characterization of a naturally occurring allopolyploid in the genus Mimulus by Sweigart et al. in this issue of Molecular Ecology is therefore to be welcomed. Mimulus is rapidly emerging as a model system for evolutionary functional genomics. Sequences of the whole genome and 200 000 expressed sequence tags of diploid M. guttatus, a putative parent of the polyploid described in this issue, will soon be available. These will facilitate investigation of the fates of genes duplicated by whole genome duplication, and their effects on morphology, mating system and ecology in natural populations. [source]

Statistical confidence in parentage analysis with incomplete sampling: how many loci and offspring are needed?

MOLECULAR ECOLOGY, Issue 5 2000
B. D. Neff
Abstract We have recently presented models to estimate parentage in breeding systems with multiple mating and incomplete sampling of the candidate parents. Here we provide formulas to calculate the statistical confidence and the optimal trade-off between the number of loci and offspring. These calculations allow an understanding of the statistical significance of the parentage estimates as well as the appropriate sampling regime required to obtain a desired level of confidence. We show that the trade-off generally depends on the parentage of the putative parents. When parentage is low, sampling effort should concentrate on increasing the number of loci. Otherwise, there are similar benefits from increasing the number of loci or offspring. We demonstrate these methods using genetic data from a nest of the bluegill sunfish (Lepomis macrochirus). [source]

Genetic diversity in hazelnut (Corylus avellana L.) cultivars from Black Sea countries assessed using SSR markers

PLANT BREEDING, Issue 4 2010
K. Gürcan
With 6 figures and 6 tables Abstract European hazelnut (Corylus avellana L.) is an important crop in Turkey, Georgia and Azerbaijan, where cultivars were selected from the native vegetation. Accessions from Turkey have been assigned to the Black Sea group, and cultivars from Georgia and Azerbaijan have a similar phenotype. Genetic diversity was investigated in 88 accessions from these three countries and compared with cultivars from Spain and Italy using 12 microsatellite loci. A high level of genetic diversity (He = 0.71, Ho = 0.70) was observed in the Black Sea accessions. Six Turkish accessions in the US hazelnut collections were found to be synonyms of cultivars in the Turkish collection in Giresun. An unweighted pair-group method using arithmetic average dendrogram and principal component analysis of 109 unique accessions showed a tendency to form subgroups by country of origin, and high diversity within each subgroup. A moderate shift in allelic frequencies (FST = 0.114,0.131) was seen between accessions from the Black Sea and the Spanish-Italian accessions. Simple sequence repeat analysis identified the putative parents of two Turkish cultivars. [source]

Development of a 25-plex SNP assay for traceability in cattle

ANIMAL GENETICS, Issue 3 2009
B. Karniol
Summary Single nucleotide polymorphisms (SNPs) are amenable to automation and therefore have become the marker of choice for DNA profiling. SNaPshot, a primer extension-based method, was used to multiplex 25 SNPs that have been previously validated as useful for identity control. Detection of extended products was based on four different fluorochromes and extension primers with oligonucleotide tails of differing lengths, thus controlling the concise length of the entire chromatogram to 81 bases. Allele frequencies for Holstein, Simmental, Limousin, Angus, Charolais and Tux Cattle were estimated and significant positive Pearson-correlation coefficients were obtained among the analysed breeds. The probability that two randomly unrelated individuals would share identical genotypes for all 25 loci varied from 10,8 to 10,10 for these breeds. For parentage control, the exclusion power was found to be 99.9% when the genotypes of both putative parents are known. A traceability test of duplicated samples indicated a high genotyping precision of >0.998. This was further corroborated by analysis of 60 cases of parent,sib pairs and trio families. The 25-plex SNaPshot assay is adapted for low- and high-throughput capacity and thus presents an alternative for DNA-based traceability in the major commercial cattle breeds. [source]