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PSA mRNA (psa + mrna)

Distribution by Scientific Domains

Medical Sciences	100%

Selected Abstracts

Adenoid cystic carcinoma of the prostate: A case report with immunohistochemical and in situ hybridization staining for prostate-specific antigen

INTERNATIONAL JOURNAL OF UROLOGY, Issue 8 2001
Sadatsugu Minei
Abstract A 43-year-old man with urinary outlet obstruction was referred to our hospital. A digital rectal examination revealed an elastic hard prostate. The serum prostate-specific antigen (PSA), serum prostatic acid phosphate and ,-seminoprotein levels were found to be within the normal range, and transrectal ultrasound sonography provided normal findings. The patient underwent a subcapsular prostatectomy under a diagnosis of benign prostatic hyperplasia. Histopathologically, the lesion was diagnosed as an adenoid cystic carcinoma of the prostate. Because a further examination revealed a pathologic extension into the urinary bladder, a radical cystoprostatectomy was performed. The expression of PSA protein and PSA mRNA was studied by means of immunohistochemistry and an in situ hybridization technique. The adenoid cystic carcinoma in the patient did not show any positive signs for PSA protein or PSA mRNA. [source]

Androgen receptor or estrogen receptor-, blockade alters DHEA-, DHT-, and E2 -induced proliferation and PSA production in human prostate cancer cells

THE PROSTATE, Issue 11 2007
Julia T. Arnold
Abstract BACKGROUND Dehydroepiandrosterone (DHEA) is an endogenous steroid that is metabolized to androgens and/or estrogens in the human prostate. DHEA levels decline with age, and use of DHEA supplements to retard the aging process is of unproved effectiveness and safety. LNCaP and LAPC-4 prostate cancer cells were used to determine whether DHEA-modulated proliferation and prostate specific antigen (PSA) production were mediated via the androgen receptor (AR) and/or ER,. METHODS Cells were treated with DHEA, DHT, or E2 and antagonists to AR (Casodex®-bicalutamide) or ER (ICI 182,780) or siRNA to the respective receptors. Proliferation was assessed by MTT assay and PSA mRNA and protein secretion were measured by quantitative real-time PCR and ELISA. Associations of AR and ER, were analyzed by co-immunoprecipitation studies and fluorescent confocal microscopy. RESULTS DHEA-, T-, and E2 -induced proliferation of LNCaP cells was blunted by Casodex but not by ICI treatment. In LNCaP cells, Casodex and ICI suppressed hormone-induced PSA production. In LAPC-4 cells, DHT-stimulated PSA mRNA was inhibited by Casodex and ICI, and the minimal stimulation by DHEA was inhibited by ICI. Use of siRNAs confirmed involvement of AR and ER, in hormone-induced PSA production while AR-ER, co-association was suggested by immunoprecipitation and nuclear co-localization. CONCLUSIONS These findings support involvement of both AR and ER, in mediating DHEA-, DHT-, and E2 -induced PSA expression in prostate cancer cells. Prostate 67: 1152,1162, 2007. © 2007 Wiley-Liss, Inc. [source]

Analysis of peripheral blood for prostate cells after autologous transfusion given during radical prostatectomy

BJU INTERNATIONAL, Issue 3 2005
John T. Stoffel
OBJECTIVES To determine if cells expressing prostate-specific antigen (PSA) can be detected in blood collected by a cell-saver during radical prostatectomy (RP) or in the peripheral blood after intraoperative autotransfusion (IAT). PATIENTS AND METHODS In all, 112 men with clinical T1c,T2 prostate cancer undergoing RP were prospectively assessed. A cell-saver system was used in each to collect blood from the surgical field after prostate manipulation. IAT was given based on clinical indications. Standardized peripheral blood samples were collected from patients before RP, in the recovery room afterward, and at 3,5 weeks after surgery. A reverse-transcriptase-polymerase chain reaction assay for PSA mRNA was used to detect prostate cells in cell-saver and peripheral blood samples. Patients were followed after surgery with PSA measurements to assess biochemical failure. RESULTS PSA-expressing cells were detected in 88% of cell-saver reservoir and 13% of preoperative blood samples. No PSA-expressing prostate cells were detected in any peripheral blood samples collected 3,5 weeks after surgery. Analysis of data with 40 months of follow-up showed IAT was not an independent predictor of biochemical failure in multivariate analysis. CONCLUSIONS Although IAT blood contains PSA-expressing cells, none could be detected 3,5 weeks after surgery. IAT during RP was not associated with a greater risk of biochemical failure. [source]