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Protection Analysis (protection + analysis)

Distribution by Scientific Domains

Life Sciences	25%

Selected Abstracts

LOPA misapplied: Common errors can lead to incorrect conclusions,,

PROCESS SAFETY PROGRESS, Issue 4 2009
Karen A. Study
Abstract Layer of Protection Analysis is a powerful tool for quantitative risk assessments. If applied correctly, it can provide quick and efficient guidance on what additional safeguards are needed, if any, to protect against a given scenario. If misapplied, an overly conservative calculation of risk may result in over-instrumentation, additional life-cycle costs, and spurious trips. A nonconservative calculation of risk could result in an under-protected system and unacceptable risk of an undesired consequence occurring. This article describes several categories of common errors, some overly conservative and some nonconservative. Case studies of actual plant scenarios are used to illustrate. © 2009 American Institute of Chemical Engineers Process Saf Prog 2009 [source]

Improved integration of LOPA with HAZOP analyses,

PROCESS SAFETY PROGRESS, Issue 4 2009
Dick Baum
Abstract Integrating Layer of Protection Analysis (LOPA) with Hazard and Operability Analysis (HAZOP) has many advantages over performing these studies separately. The merits include: fewer actions from the combined effort compared to performing only a HAZOP; team continuity resulting from the combined effort as opposed to two separate teams having possibly differing points of view; and, ultimately, a time and cost savings realized by the combination. This integration defines the risk associated with a given scenario, enabling better decisions that impact business assurance. By using the Center for Chemical Process Safety guidelines to define the independent protection layers upfront, the gray areas can often be reduced or eliminated; thereby enabling a more thorough LOPA. Examples include taking credit if a unit has two independent operators (outside and inside) responding to critical alarms, or taking credit for centralized control rooms that may allow immediate operator interaction and response. This article shows how the guidelines have been used successfully in joint HAZOP/LOPA studies, and describes an initial preparation protocol that can ensure high-quality results. © 2009 American Institute of Chemical Engineers Process Saf Prog, 2009 [source]

Initiating events and independent protection layers

PROCESS SAFETY PROGRESS, Issue 4 2009
John F. Murphy
Abstract Layer of protection analysis (LOPA) is a semiquantitative tool for analyzing and assessing process risk. The tool has grown greatly in popularity and usefulness since the publication of the first CCPS/AIChE guidebook on the subject, Layer of Protection Analysis, Simplified Process Risk Assessment (LOPA). This article is a summary of a new CCPS guideline book that includes the following: Initiating events (IEs). Independent protection layers (IPLs). Criteria for identifying the value of each prospective IE and prospective IPL. Example IE and IPL data. This book is a necessary reference for those applying the LOPA methodology. © 2009 American Institute of Chemical Engineers Process Saf Prog, 2009 [source]

Translational regulation of a novel testis-specific RNF4 transcript

MOLECULAR REPRODUCTION & DEVELOPMENT, Issue 1 2003
Raffaela Pero
Abstract The RING-finger protein SNURF/RNF4, a modulator of both steroid receptor dependent and basal transcription, is expressed at very high levels in testis and at much lower levels in several other tissues. In somatic tissues, the RNF4 gene is expressed as a 3-kb transcript while an additional shorter sized transcript (1.6 kb) was found in mouse testis. In murine germ cells, RNF4 protein expression is strongly modulated during progression of spermatogonia to spermatids, with a peak in spermatocytes. The expression of 3-kb transcript correlated with protein levels in the different germ cell populations. Conversely, the 1.6-kb transcript was abundantly and specifically expressed in spermatids, in which RNF4 protein was detected at very low levels. We have then examined possible mechanisms underlying this discrepancy. Primer extension and RNase protection analyses demonstrated that the 1.6- and 3.0-kb transcripts originate from the same promoter, encode for the same protein and differ in the 3, UTR. In vitro assays showed that protein degradation is not involved in the regulation of RNF4 protein level. Finally, polysome analysis revealed that only a slight fraction of the testis-specific transcript is engaged in translation, thus providing a feasible mechanism for the quantitative differences of RNF4 mRNA and protein levels. Present results demonstrate that RNF4 short transcript is poorly translated suggesting that this mechanism could be essential for normal spermatogenesis. Mol. Reprod. Dev. 66: 1,7, 2003. © 2003 Wiley-Liss, Inc. [source]

Identification of BOIP, a novel cDNA highly expressed during spermatogenesis that encodes a protein interacting with the orange domain of the hairy-related transcription factor HRT1/Hey1 in Xenopus and mouse

DEVELOPMENTAL DYNAMICS, Issue 4 2003
Reginald Van Wayenbergh
Abstract Hairy-related transcription factor (HRT/Hey) genes encode a novel subfamily of basic helix-loop-helix (bHLH) transcription factors related to the Drosophila hairy and Enhancer-of-split (E(spl)) and the mammalian HES proteins that function as downstream mediators of Notch signaling. Using the yeast two-hybrid approach, a previously uncharacterized protein was identified in Xenopus that interacts with XHRT1 (originally referred to as bc8), one member of the HRT/Hey subclass. This protein is evolutionarily conserved in chordates. It binds to sequences adjacent to the bHLH domain of XHRT1 known as the Orange domain and has been named bc8 Orange interacting protein (BOIP). BOIP shows a rather uniform subcellular localization and is recruited to the nucleus upon binding to XHRT1. In Xenopus, XBOIP mRNA is detected by RNase protection analysis throughout embryogenesis. In the adult, the strongest expression is detected in testis. In the mouse, high levels of BOIP mRNA are also found in adult testis. No expression is detected in the embryo and in any of the other adult organs tested. In situ hybridization revealed that BOIP transcripts were detected almost exclusively in round spermatids and that this expression overlaps with that of Hey1 (HRT1), which is expressed throughout spermatogenesis. In view of the importance of the Orange domain for HRT/Hey function, the newly identified BOIP proteins may serve as regulators specifically of HRT1/Hey1 activity. Developmental Dynamics 228:716,725, 2003. © 2003 Wiley-Liss, Inc. [source]

Initiating events and independent protection layers

Using layer of protection analysis to define safety integrity level requirements

PROCESS SAFETY PROGRESS, Issue 3 2007
Raymond Freeman
The recent publication of ANSI/ISA standard S84.01 , 2004 defines the life safety cycle for the design and installation of safety instrumented functions (SIFs) using safety instrumented systems (SISs). However, the determination of the required safety integrity level (SIL) is an activity that the process designers need to complete prior to detailed design. This article outlines an approach for the determination of the required SIL using the layer of protection analysis (LOPA) method. An example is presented along with a suggested format for documenting the LOPA, SIL, and SIF requirements in a manner that can be transferred to the detailed design team for implementation. © 2007 American Institute of Chemical Engineers Process Saf Prog, 2007 [source]