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Atom Bombardment Mass Spectrometry (atom + bombardment_mass_spectrometry)

Distribution by Scientific Domains
Chemistry75%

Kinds of Atom Bombardment Mass Spectrometry

  • fast atom bombardment mass spectrometry
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    Selected Abstracts

    Recombinant glycodelin carrying the same type of glycan structures as contraceptive glycodelin-A can be produced in human kidney 293 cellsbut not in Chinese hamster ovary cells

    FEBS JOURNAL, Issue 15 2000
    Ingrid M. Van den Nieuwenhof
    We have produced human recombinant glycodelin in human kidney 293 cells and in Chinese hamster ovary (CHO) cells. Structural analyses by lectin immunoassays and fast atom bombardment mass spectrometry showed that recombinant human glycodelin produced in CHO cells contains only typical CHO-type glycans and is devoid of any of the N,N,- diacetyllactosediamine (lacdiNAc)-based chains previously identified in glycodelin-A (GdA). By contrast, human kidney 293 cells produced recombinant glycodelin with the same type of carbohydrate structures as GdA. The presence of a ,1,4- N- acetylgalactosaminyltransferase functioning in the synthesis of lacdiNAc-based glycans in human kidney 293 cells is concluded to be the cause of the occurrence of lacdiNAc-based glycans on glycodelin produced in these cells. Furthermore, human kidney 293 cells were found to be particularly suited for the production of recombinant glycodelin when they were cultured in high glucose media. Lowering the glucose concentration and the addition of glucosamine resulted in higher relative amounts of oligomannosidic-type glycans and complex glycans with truncated antennae. Human glycodelin is an attractive candidate for the development of a contraceptive agent, and this study gives valuable information for selecting the proper expression system and cell culture conditions for the production of a correctly glycosylated recombinant form. [source]

    Discrimination between pentose oligosaccharides containing D -xylopyranose or L -arabinofuranose as non-reducing terminal residue using fast atom bombardment mass spectrometry,

    JOURNAL OF MASS SPECTROMETRY (INCORP BIOLOGICAL MASS SPECTROMETRY), Issue 4 2001
    Vladimír Ková
    Abstract Collisional-induced dissociation (CID) mass spectra of the [M + H]+ and [M , H], ions obtained under fast atom bombardment conditions of a number of methyl glycoside di-, tri- and tetrasaccharides, containing D -xylopyranosyl and/or L -arabinofuranosyl residues at the non-reducing terminus, do not provide information about their ring size. This information could only be obtained from a careful comparison of the intensity ratio of the [M + Na , 90]+ and [M + Na , 104]+ ions (0,2Xt/1,5Xt) in the high-energy CID spectra of the sodium-cationized di-, tri- and probably also tetrasaccharide compounds. Copyright © 2001 John Wiley & Sons, Ltd. [source]

    Organosoluble star polymers from a cyclodextrin core

    JOURNAL OF POLYMER SCIENCE (IN TWO SECTIONS), Issue 21 2005
    Khaled Karaky
    Abstract Well-defined star polymers were synthesized with a combination of the core-first method and atom transfer radical polymerization. The control of the architecture of the macroinitiator based on ,-cyclodextrin bearing functional bromide groups was determined by 13C NMR, fast atom bombardment mass spectrometry, and elemental analysis. In a second step, the polymerization of the tert -butyl acrylate monomer was optimized to avoid a star,star coupling reaction and allowed the synthesis of a well-defined organosoluble polymer star. The determination of the macromolecular dimensions of these new star polymers by size exclusion chromatography/light scattering was in agreement with the structure of armed star polymers in a large range of predicted molecular weights. This article describes a new approach to polyelectrolyte star polymers by postmodification of poly(tert -butyl acrylate) by acrylic arm hydrolysis in a water-soluble system. © 2005 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 43: 5186,5194, 2005 [source]

    A new dihydroflavonol from Pinus sylvestris L.

    MAGNETIC RESONANCE IN CHEMISTRY, Issue 4 2005
    Jari Sinkkonen
    Abstract A novel dihydroflavonol, C-6,O-7 -dimethylaromadendrin, was isolated from a 70% aqueous acetone extract of pine (Pinus sylvestris L.) bark. Its structure was determined by high-resolution negative fast atom bombardment mass spectrometry and NMR spectroscopy. The complete assignment of proton and carbon signals was achieved by 2D NMR experiments: HSQC, HMBC, DQF-COSY and NOESY. Copyright © 2005 John Wiley & Sons, Ltd. [source]