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Processing Rate (processing + rate)
Selected AbstractsMechanisms of functional response and resource exploitation in browsing roe deerJOURNAL OF ANIMAL ECOLOGY, Issue 5 2002Andrew W. Illius Summary 1The functional responses of roe deer were examined using 11 plant species. A technique to discriminate between encounter- and handling-limited processes was used, and it can be concluded that the functional response applicable to patch browsing by roe deer is governed not by the rate of encounter but by the rate of oral processing. 2The large differences between plant species were due to variations in both parameters of the functional response: h , the time lost in biting, and Rmax , the maximum processing rate. Removing the thorns from three of the species affected these parameters differently, according to the size and density of thorns. 3Animals took larger bites from larger patches (branches), and bite mass declined as patch exploitation progressed, implying that animals were selecting the larger items to eat first. It was demonstrated experimentally that depletion of the larger bites does occur first, and it was concluded that prey selection is an important component of herbivore foraging behaviour. 4The gain curves for deer feeding on the different plant species are calculated as being virtually linear. Patch depression did not, in general, occur because increasing bite rate compensated for declining bite mass. Our mechanistic approach is contrasted with other approaches to describing the gain curve in the literature. 5A priori and empirical grounds are presented for rejecting the hypothesis that resource exploitation by browsing mammals is governed by optimal patch use. Diet optimization, involving a trade-off between diet quality and quantity, offers a better explanation of herbivore foraging behaviour. [source] Hyperosmotic Stress in Murine Hybridoma Cells: Effects on Antibody Transcription, Translation, Posttranslational Processing, and the Cell CycleBIOTECHNOLOGY PROGRESS, Issue 2 2004Zhe Sun Mechanisms for increased antibody production in batch cultures of murine hybridoma cells in response to hyperosmotic stress were investigated. The rates of immunoglobulin transcription and protein translation and posttranslational processing were determined in control and hyperosmotic cultures. Changes in immunoglobulin transcription played a minor role in the increase in antibody production in response to hyperosmotic stress. In contrast, protein translation increased substantially in response to osmotic stress. However, the antibody translation rate remained relatively constant after correcting for the overall increase in protein translation. Cell size and intracellular antibody pool also increased in response to hyperosmolarity. The intracellular antibody pool increased proportionately with the increase in cell size, indicating that hyperosmotic cultures do not selectively increase their intracellular antibody population. Changes in cell cycle distribution in response to osmotic stress and the relationship between the cell cycle and antibody production were also evaluated. Hyperosmotic stress altered the cell cycle distribution, increasing the fraction of the cells in S-phase. However, this change was uncorrelated with the increase in antibody production rate. Immunoglobulin degradation was relatively low (,15%) and remained largely unchanged in response to hyperosmotic stress. There was no apparent increase in immunoglobulin stability as a result of osmotic stress. Antibody secretion rates increased approximately 50% in response to osmotic stress, with a commensurate increase in the antibody assembly rate. The rate of transit through the entire posttranslational processing apparatus increased, particularly for immunoglobulin light chains. The levels of endoplasmic reticulum chaperones did not increase as a fraction of the total cellular protein but were increased on a per cell basis as the result of an increase in total cellular protein. A difference in the interactions between the immunoglobulin heavy chains and BiP/GRP78 was observed in response to hyperosmotic conditions. This change in interaction may be correlated with the decrease in transit time through the posttranslational pathways. The increase in the posttranslational processing rate appears to be commensurate with the increase in antibody production in response to hyperosmotic stress. [source] Development of a Segmented Model for a Continuous Electrophoretic Moving Bed Enantiomer SeparationBIOTECHNOLOGY PROGRESS, Issue 6 2003Brian M. Thome With the recent demonstration of a continuous electrophoretic "moving bed" enantiomer separation at mg/h throughputs, interest has now turned to scaling up the process for use as a benchtop pharmaceutical production tool. To scale the method, a steady-state mathematical model was developed that predicts the process response to changes in input feed rate and counterflow or "moving bed" velocities. The vortex-stabilized apparatus used for the separation was modeled using four regions based on the different hydrodynamic flows in each section. Concentration profiles were then derived on the basis of the properties of the Piperoxan-sulfated ,-cyclodextrin system being studied. The effects of different regional flow rates on the concentration profiles were evaluated and used to predict the maximum processing rate and the hydrodynamic profiles required for a separation. Although the model was able to qualitatively predict the shapes of the concentration profiles and show where the theoretical limits of operation existed, it was not able to quantitatively match the data from actual enantiomer separations to better than 50% accuracy. This is believed to be due to the simplifying assumptions involved, namely, the neglect of electric field variations and the lack of a competitive binding isotherm in the analysis. Although the model cannot accurately predict concentrations from a separation, it provides a good theoretical framework for analyzing how the process responds to changes in counterflow rate, feed rate, and the properties of the molecules being separated. [source] Effects of drying regime on microbial colonization and shredder preference in seasonal woodland wetlandsFRESHWATER BIOLOGY, Issue 3 2008MARTYN D. INKLEY Summary 1. Energy budgets of wetlands in temperate deciduous forests are dominated by terrestrially derived leaf litter that decays under different drying conditions depending on autumn precipitation. We compared decay rates and microbial colonization of maple leaves under different inundation schedules in a field experiment, and then conducted a laboratory study on shredder preference. In the field, litter bags either remained submerged (permanent), were moved to a dried part of the basin once and then returned (semi-permanent), or were alternated between wet and dry conditions for 8 weeks (temporary). 2. There was no difference in decay rates among treatments, but leaves incubated under permanent and semi-permanent conditions had higher fungal and bacterial biomass, and lower C : N ratios than those incubated under alternating drying and wetting conditions. 3. To determine the effects of these differences in litter nutritional quality on shredder preference, we conducted a laboratory preference test with larvae of leaf-shredding caddisflies that inhabit the wetland. Caddisflies spent twice as much time foraging on permanent and semi-permanent litter than on litter incubated under temporary conditions. 4. There is considerable variation among previous studies in how basin drying affects litter breakdown in wetlands, and no previous information on shredder preference. We found that frequent drying in a shallow wetland reduces the nutritional quality of leaf litter (lower microbial biomass and nitrogen content), and therefore preference by invertebrate shredders. These results suggest that inter-annual shifts in drying regime should alter detritus processing rates, and hence the mobilization of the energy and nutrients in leaf litter to the wetland food web. [source] Evaluation of macrofaunal effects on leaf litter breakdown rates in aquatic and terrestrial habitatsAUSTRAL ECOLOGY, Issue 6 2006AUGUSTO C. DE A. RIBAS Abstract Decomposition of the organic matter is a key process in the functioning of aquatic and terrestrial ecosystems, although different factors influence processing rates between and within these habitats. Most patterns were described for temperate regions, with fewer studies in tropical, warmer sites. In this study, we carried out a factorial experiment to compare processing rates of mixed species of leaf litter between terrestrial and aquatic habitats at a tropical site, using ,ne and coarse mesh cages to allow or prevent colonization by macroinvertebrates. The experiment was followed for 10 weeks, and loss of leaf litter mass through time was evaluated using exponential models. We found no interaction between habitat and mesh size and leaf litter breakdown rates did not differ between ,ne and coarse mesh cages, suggesting that macroinvertebrates do not influence leaf litter decomposition in either habitat at our studied site. Leaf breakdown rates were faster in aquatic than in terrestrial habitats and the magnitude of these differences were comparable to studies in temperate regions, suggesting that equivalent factors can influence between-habitat differences detected in our study. [source] |