ProRoot MTA (proroot + mta)

Distribution by Scientific Domains

Selected Abstracts

Apatite-forming ability (bioactivity) of ProRoot MTA

M. G. Gandolfi
Gandolfi MG, Taddei P, Tinti A, Prati C. Apatite-forming ability (bioactivity) of ProRoot MTA. International Endodontic Journal, 43, 917,929, 2010. Abstract Aim, Apatite-forming ability, considered as an index of bioactivity (bond-to-bone ability), was tested on ProRoot MTA cement after immersion in phosphate-containing solution (DPBS). Methodology, Disk samples were prepared and immersed in DPBS for 10 min, 5 h, 1 and 7 days. The cement surface was studied by attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, by micro-Raman spectroscopy and by environmental scanning electron microscope with energy dispersive X-ray (ESEM-EDX) analyses. The pH of the storage solution was also investigated. Results, Spectroscopic analyses revealed calcium phosphate bands after 5-h immersion in DPBS. After 1 day, an even coating composed of apatite spherulites (0.1,0.8 micron diameter) was observed by ESEM/EDX. After 7 days, its thickness had increased. Apatite nucleation had already occurred after 5-h immersion. At this time, the presence of portlandite (i.e. Ca(OH)2, calcium hydroxide) on the cement surface was also observed; at longer times, this component was released into the medium, which underwent a remarkable pH increase. Conclusions, The study confirms the ability of ProRoot MTA to form a superficial layer of apatite within hours. The excellent bioactivity of ProRoot MTA might provide a significant clinical advantage over the traditional cements used for root-end or root-perforation repair. [source]

The effect of pH on surface hardness and microstructure of mineral trioxide aggregate

M. S. Namazikhah
Abstract Aim, To evaluate the surface microhardness of mineral trioxide aggregate (MTA) specimens following exposure of their surface to a range of acidic environments during hydration. In addition, the morphological microstructure features of samples were studied by scanning electron microscopy (SEM). Methodology, White ProRoot MTA (Dentsply Tulsa Dental, Johnson City, TN, USA) was mixed and packed into cylindrical polycarbonate tubes. Four groups, each of 10 specimens, were formed using a pressure of 3.22 MPa and exposed to pH 4.4, 5.4, 6.4 and 7.4, respectively, for 4 days. Vickers microhardness of the surface of each specimen was measured after exposure. Four groups of two specimens were prepared and treated in the same way prior to qualitative examination by SEM. Data were subjected to one-way anova and post hoc Tukey's test. Result, The greatest mean surface hardness values (53.19 ± 4.124) were observed following exposure to pH 7.4 with the values decreasing to 14.34 ± 6.477 following exposure to pH 4.4. The difference between these values at the 95% CI (33.39,44.30) was statistically significant (P < 0.0001). There were no distinct morphological differences between groups in terms of the internal microstructure. However, a trend was observed that the more acidic the solution, the more extensive the porosity of the specimens. Conclusion, Under the conditions of this study, surface hardness of MTA was impaired in an acidic environment. [source]

Prostaglandin E2 production and viability of cells cultured in contact with freshly mixed endodontic materials

K. K. Melegari
Abstract Aim, To determine whether commonly used endodontic sealers could either induce or increase the release of prostaglandin E2 (PGE2) when in contact with cell types found in the periapical tissues. Methodology, Freshly mixed samples of Roth 801 sealer, Sealapex® and ProRoot® mineral trioxide aggregate (MTA) were placed in contact with cultured macrophages and fibroblasts for 24 h. The supernatant from the cultures was assayed for PGE2 using enzyme-linked immunosorbent assay. Cell viability counts were made. As a positive control, similar cultures were also exposed to lipopolysaccharide and the supernatant analysed for PGE2. Data were compared by anova. Results, The three materials examined in these experiments did not stimulate increased PGE2 release from either of the cell lines. In control cultures, lipolysaccharide increased PGE2 release from macrophages but not from fibroblasts. Viability counts revealed that, whilst Roth 801 sealer caused some cell death in both fibroblasts and macrophages, Sealapex® led to cell death only in the macrophage cultures. ProRoot® MTA did not lead to statistically significant cell death in either culture. Conclusions, Under 24-h culture conditions, the three freshly mixed test materials did not increase directly either production or release of PGE2 from either macrophages or gingival fibroblasts. Roth 801 decreased cell viability counts for both fibroblasts and macrophages. Sealapex® decreases macrophage viability. ProRoot® MTA did not affect viability in either cell line. [source]

Scanning electron microscopy evaluation of the hard tissue barrier after pulp capping with calcium hydroxide, mineral trioxide aggregate (MTA) or ProRoot MTA

Eduardo Galia Reston dds
Abstract The aim of this study was to investigate the morphology and localisation of calcium hydroxide- and mineral trioxide aggregate (MTA)-induced hard tissue barriers after pulpotomy in dogs' teeth. Pulpotomies were performed on maxillary and mandibular premolars of five dogs. The teeth were assigned into three groups according to the pulp-capping agent used. The pulpal wounds were capped with calcium hydroxide (Ca(OH)2, control), MTA or ProRoot MTA, and the cavities were restored with amalgam. After a 90-day follow-up period, the dogs were euthanised and the teeth were examined under scanning electron microscopy (SEM). An image-processing and analysis software was used to delimit the perimeters of the root canal area and the hard tissue barrier to determine the percentage of root canal obliteration. SEM data were used to assess the morphology, localisation and extension of the reparative hard tissue barriers. ProRoot MTA was statistically different from MTA and Ca(OH)2 (P < 0.05) regarding tissue barrier morphology. Localisation data showed that ProRoot MTA was significantly different from Ca(OH)2 (P < 0.05) and similar to MTA (P > 0.01; P > 0.05). No statistically significant difference (P > 0.01; P > 0.05) was observed between MTA and Ca(OH)2. A larger number of complete (centroperipheral) hard tissue barriers with predominance of dentinal tubules was observed to the ProRoot MTA when compared with the Ca(OH)2 group. [source]