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Pretreatment Process (pretreatment + process)

Distribution by Scientific Domains

Life Sciences	42%
Chemistry	42%

Selected Abstracts

Effect of varying feedstock,pretreatment chemistry combinations on the formation and accumulation of potentially inhibitory degradation products in biomass hydrolysates

BIOTECHNOLOGY & BIOENGINEERING, Issue 3 2010
Bowen Du
Abstract A variety of potentially inhibitory degradation products are produced during pretreatment of lignocellulosic biomass. Qualitative and quantitative interrogation of pretreatment hydrolysates is paramount to identifying potential correlations between pretreatment chemistries and microbial inhibition in downstream bioconversion processes. In the present study, corn stover, poplar, and pine feedstocks were pretreated under eight different chemical conditions, which are representative of leading pretreatment processes. Pretreatment processes included: 0.7% H2SO4, 0.07% H2SO4, liquid hot water, neutral buffer solution, aqueous ammonia, lime, lime with oxygen pressurization, and wet oxidation. Forty lignocellulosic degradation products resulting from pretreatment were analyzed using high performance liquid chromatography in combination with UV spectroscopy or tandem mass spectrometry detection (HPLC-PDA-MS/MS) and ion chromatography (IC). Of these compounds, several have been reported to be inhibitory, including furfural, hydroxymethyl furfural, ferulic acid, 3,4-dihydroxybenzaldehyde, syringic acid among others. Formation and accumulation of monitored compounds in hydrolysates is demonstrated to be a function of both the feedstock and pretreatment conditions utilized. Biotechnol. Bioeng. 2010;107: 430,440. © 2010 Wiley Periodicals, Inc. [source]

Impact of dilute acid pretreatment on the structure of bagasse for bioethanol production

INTERNATIONAL JOURNAL OF ENERGY RESEARCH, Issue 3 2010
Wei-Hsin Chen
Abstract Dilute acid pretreatment is a commonly used pretreatment method in the course of producing bioethanol from lignocellulosics and the structure variation of the lignocellulosics is highly related to the pretreatment process. To understand the impact of dilute acid pretreatment on the structure of bagasse, four different pretreatment conditions by varying heating time are considered where the bagasse and the pretreated materials are examined using a variety of analysis methods. The obtained results indicate that the thermogravimetric analysis (TGA) is able to provide a useful insight into the recognition of lignocellulosic structure. Specifically, the peak of the TGA of the pretreated materials moves toward the low temperature region, revealing that the lignocellulosic structure is loosened. However, the characteristic of crystal structure of cellulose remains in the pretreated materials. Increasing heating time enhances the pretreatment procedure; as a result, the average particle size of the investigated materials increases with heating time. This swelling behavior may be attributed to the enlarged holes inside the particles in that the surface area decreases with increasing heating time. In addition, when the heating time is increased to a certain extent (e.g. 15,min), some fragments are found at the surface and they tend to peel off from the surface. It follows that the dilute acid pretreatments have a significant effect on the bagasse structure. Copyright © 2009 John Wiley & Sons, Ltd. [source]

Stabilization of biomass-derived pyrolysis oils

JOURNAL OF CHEMICAL TECHNOLOGY & BIOTECHNOLOGY, Issue 5 2010
R.H. Venderbosch
Abstract BACKGROUND: Biomass is the only renewable feedstock containing carbon, and therefore the only alternative to fossil-derived crude oil derivatives. However, the main problems concerning the application of biomass for biofuels and bio-based chemicals are related to transport and handling, the limited scale of the conversion process and the competition with the food industry. To overcome such problems, an integral processing route for the conversion of (non-feed) biomass (residues) to transportation fuels is proposed. It includes a pretreatment process by fast pyrolysis, followed by upgrading to produce a crude-oil-like product, and finally co-refining in traditional refineries. RESULTS: This paper contributes to the understanding of pyrolysis oil upgrading. The processes include a thermal treatment step and/or direct hydroprocessing. At temperatures up to 250 °C (in the presence of H2 and catalyst) parallel reactions take place including re-polymerization (water production), decarboxylation (limited CO2 production) and hydrotreating. Water is produced in small quantities (approx. 10% extra), likely caused by repolymerization. This repolymerization takes place faster (order of minutes) than the hydrotreating reactions (order of tens of minutes, hours). CONCLUSIONS: In hydroprocessing of bio-oils, a pathway is followed by which pyrolysis oils are further polymerized if H2 and/or catalyst is absent, eventually to char components, or, with H2/catalyst, to stabilized components that can be further upgraded. Results of the experiments suggest that specifically the cellulose-derived fraction of the oil needs to be transformed first, preferably into alcohols in a ,mild hydrogenation' step. This subsequently allows further dehydration and hydrogenation. Copyright © 2010 Society of Chemical Industry [source]

Therapeutic monitoring of imipramine and desipramine by micellar liquid chromatography with direct injection and electrochemical detection

BIOMEDICAL CHROMATOGRAPHY, Issue 5 2005
Devasish Bose
Abstract A micellar liquid chromatographic (MLC) procedure was developed for the clinical monitoring of imipramine and its active metabolite, desipramine. The determination of these highly hydrophobic substances was carried out after direct injection of the serum samples using a mobile phase composed of 0.15 m SDS,6% (v/v) pentanol buffered at pH 7, pumped at 1.5 mL/min into a C18 column (250 × 4.6 mm), and electrochemical detection at 650 mV. Using this MLC method, calibration was linear (r > 0.995) and the limits of detection (ng/mL) were 0.34 and 0.24 for imipramine and desipramine, respectively. Repeatabilities and intermediate precision were tested at three different concentrations in the calibration range and a CV (%) below 2.2 was obtained. In this MLC procedure, the serum is determined without treatment, thus allowing repeated serial injections without changes in retention factors, and reducing the time and consumables required to carry out the pretreatment process. The assay method can be applied to the routine determination of serum imipramine and its metabolite in therapeutic drug monitoring. Copyright © 2004 John Wiley & Sons, Ltd. [source]

Pretreatment of hybrid poplar by aqueous ammonia

BIOTECHNOLOGY PROGRESS, Issue 2 2009
Rajesh Gupta
Abstract Enzymatic hydrolysis of hybrid poplar treated by ammonia recycle percolation (ARP) was studied applying cellulase enzyme supplemented with additional xylanase or pectinase. The effect of xylanase addition was much more significant than pectinase addition. Conversion of ARP-treated hybrid poplar to ethanol was carried out by simultaneous saccharification and fermentation (SSF) and SS and cofermentation (SSCF). The maximum ethanol yield observed from the SSCF experiment was 78% of theoretical maximum based on the total carbohydrate (glucan + xylan). The same feedstock was also treated by soaking in aqueous ammonia (SAA), a batch pretreatment process with lower severity than ARP. The test results indicated that relatively high severity is required to attain acceptable level of digestibility of hybrid poplar. In order to lower the severity of the pretreatment, addition of H2O2 was attempted in the SAA. Addition of H2O2 significantly enhanced delignification of hybrid poplar due to its oxidative degradation of lignin. Several different H2O2 feeding schemes and different temperature profiles were attempted in operation of the SAA to investigate the effects of H2O2 on degradation of lignin and carbohydrates in hybrid poplar. More than 60% of lignin in hybrid poplar was removed with stepwise-increase of temperature (60,120°C after 4h of reaction). Increase of carbohydrate degradation was also observed under this condition. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009 [source]

Effect of Modified Enzymatic Catalysis on the Extraction of Diosgenin from Dioscorea zingiberensis C.,H.

CHEMICAL ENGINEERING & TECHNOLOGY (CET), Issue 11 2007
Wright
Abstract Multi-enzymatic catalysis combined with acid hydrolysis is studied in order to enhance the efficiency of the enzymatic catalysis and reduce the mass transfer resistance from starch and cellulose in the extraction of diosgenin from Dioscorea zingiberensis C.,H. Wright. The cellulase is modified by polyethylene to increase its optimal reaction temperature and pH value. The modified cellulase shows better thermostability and resistance to alkali. The modified cellulase, , -amylase and , -glycosidase are used to construct the multi-enzyme and multi-enzyme catalysis is used as a pretreatment process. Compared to primary industrial techniques including acid hydrolysis, spontaneous fermentation and enzymatic catalysis, conventional techniques are optimized by using multi-enzymatic catalysis together with acid hydrolysis because of the higher reaction efficiency and lower levels of manipulation required. The purity of the product is more than 96,% with this technique, and the melting point is 205,207,°C. The diosgenin yield rate and the extraction rate reached are 2.43,% and 98,%, respectively. IR and 1H NMR spectroscopy were used to confirm the structure of the product. [source]

Effect of varying feedstock,pretreatment chemistry combinations on the formation and accumulation of potentially inhibitory degradation products in biomass hydrolysates