Home About us Contact
|
Home About us Contact | ||
|
|
||
Predicted Molecular Weight (predicted + molecular_weight)
Selected AbstractsMolecular characterization of two novel esterase genes from carmine spider mite, Tetranychus cinnabarinus (Acarina: Tetranychidae)INSECT SCIENCE, Issue 2 2010Wei Sun Abstract, Two novel esterase complementary DNAs were identified and cloned from the insecticide-susceptible strain of Tetranychus cinnabarinus (Boisduval) (Acarina: Tetranychidae), which were designated as TCE1 and TCE2, respectively. The cDNA of TCE1 gene contained an open reading frame (ORF) of 1701 bp encoding 567 amino acids, and a predicted molecular weight of 62.75 kDa, the cDNA of TCE2 contained an ORF of 1680 bp encoding 560 amino acids, and a predicted molecular weight of 63.14 kDa. TCE1 and TCE2 were submitted to GenBank, accession number EU130461 and EU130462. The well-conserved sequence motif, GXSXG, used as a signature pattern in the esterase family are present in both TCE1 and TCE2 (GQSAG in TCE1, whereas GESAG in TCE2), indicating that these two genes are predicted to be esterases. Comparison of the deduced amino acid sequence with the published mite esterase sequence coming from Boophilus microplus showed that TCE1 shares 33.98% identity and TCE2 shares 33.46% identity. TCE1 and TCE2 share 46.4% identity. Quantitative real-time polymerase chain reaction revealed that expression level of the TCE2 gene was relatively higher than that of the TCE1 in all instars examined except the protonymph, and the expression level of these two esterase genes in adults of T. cinnabarinus was significantly higher than that in any other instars, respectively. T. cinnabarinus is an important agricultural mite pest and esterases are important in the metabolisms of insects and mites; the genomic information obtained in this study will contribute to esterase molecular biological study on mite pest species. [source] Analysis of a late gene, orf101 from Helicoverpa armigera single nucleocapsid nucleopolyhedrovirusINSECT SCIENCE, Issue 5 2005SHI-HENG AN Abstract Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus open reading frame 101 (ha101) is 762 nts in length and encodes a 254 amino acid peptide with predicted 29 kDa molecular weight. The homologues of ha101 were explored using BLASTP searching tool in the updated GenBank/EMBL and SWISS-PROT databases. The results showed that the homologues of ha101 were present in all the completely sequenced lepidopteran nucleopolyhedroviruses and granuloviruses, suggesting that ha101 might be a functional gene associated with their lepidopteran hosts. Sequence alignment of ha101 and its homologues revealed that 10 amino acids were completely conserved. RT-PCR analysis of ha101 manifested that the transcript of ha101 was first detected at 24 hpi and remained detectable at up to 122 hpi, suggesting that ha101 was transcribed during late stages of infection. Ha101 was expressed using Bac to Bac system in Tn5B-1-4 cells. The product of ha101 expressed in Tn5B-1-4 cells was approximately 29 kDa, consistent with the predicted molecular weight, and the results were confirmed by western blot analysis. The subcellular localization indicated that ha101 was aggregated along nuclear envelope during the early stages of infection and spread out to the entire nucleus including virogenic stroma in late stages of infection, suggesting that ha101 may play a specific role in virion assembly process or virogenic stroma arrangement. [source] The aggregation-promoting factor of Lactobacillus crispatus M247 and its genetic locusJOURNAL OF APPLIED MICROBIOLOGY, Issue 4 2004H. Marcotte Abstract Aims:, Characterization of the aggregation-promoting factor (APF) of the human intestinal isolate Lactobacillus crispatus M247 and its homologous nonaggregating mutant Mu5. Methods and Results:, Western blot analysis revealed that the supernatant of both M247 and Mu5 contains a 28-kDa protein which cross reacts with the antiserum produced against the APF of Lact. gasseri 4B2. The apf genes of M247 and Mu5 strains were identical and were shown to be 672 nucleotides in length and encoding a protein of 223 amino acids with a predicted molecular weight of 24·0 kDa. Conclusion:, Our results shows that the lost of aggregation in Mu5 is not related to a defect in secretion of the APF protein or a mutation in the apf gene. Significance and Impact of the Study:, These results suggest that the mutation in Mu5 may be contained in another molecule involved in aggregation such as a possible receptor for APF. [source] Zebrafish Cx35: Cloning and characterization of a gap junction gene highly expressed in the retinaJOURNAL OF NEUROSCIENCE RESEARCH, Issue 6 2003Elizabeth McLachlan Abstract The vertebrate connexin gene family encodes protein subunits of gap junction channels, which provide a route for direct intercellular communication. Consequently, gap junctions play a vital role in many developmental and homeostatic processes. Aberrant functioning of gap junctions is implicated in many human diseases. Zebrafish are an ideal vertebrate model to study development of the visual system as they produce transparent embryos that develop rapidly, thereby facilitating morphological and behavioral testing. In this study, zebrafish connexin35 has been cloned from a P1 artificial chromosome (PAC) library. Sequence analysis shows a high degree of similarity to the Cx35/36 orthologous group, which are expressed primarily in nervous tissue, including the retina. The gene encodes a 304-amino acid protein with a predicted molecular weight of approximately 35 kDa. Injection of zebrafish Cx35 RNA into paired Xenopus oocytes elicited intercellular electrical coupling with weak voltage sensitivity. In development, Cx35 is first detectable by Northern analysis and RT-PCR, at 2 days post-fertilization (2 dpf), and in the adult it is expressed in the brain and retina. Immunohistochemical analysis revealed that the Cx35 protein is expressed in two sublaminae of the inner plexiform layer of the adult retina. A similar pattern was seen in the 4 and 5 dpf retina, but no labeling was detected in the retina of earlier embryos. © 2003 Wiley-Liss, Inc. [source] Synthesis of new crosslinkable polymers by chemoselective polymerizations of 2-(1-aziridinyl)ethyl methacrylateJOURNAL OF POLYMER SCIENCE (IN TWO SECTIONS), Issue 9 2003Takashi Ishizone The anionic vinyl polymerization of 2-(1-aziridinyl)ethyl methacrylate (1) quantitatively proceeded with diphenylmethylpotassium in the presence of diethylzinc in tetrahydrofuran at ,78 °C for 12 h to form a polymethacrylate (2) with an aziridine ring in each repeating unit. The resulting polymer 2 possessed the predicted molecular weight and narrow molecular weight distribution (weight-average molecular/number-average molecular weight < 1.1) and became readily insoluble by treatment with adipic acid via the crosslinking of the pendant aziridinyl moiety. A poly(ethylene imine) bearing methacryloyl pendant groups was obtained with triethyloxonium tetrafluoroborate via the cationic ring-opening polymerization of 1 in heptane/dichloromethane at 0 °C for 2 h. [source] | ||||||||||