			Home About us Contact

Powerful Methodology (powerful + methodology)

Distribution by Scientific Domains

Business, Economics, Finance and Accounting	33%

Selected Abstracts

Targeted gene expression by the Gal4-UAS system in zebrafish

DEVELOPMENT GROWTH & DIFFERENTIATION, Issue 6 2008
Kazuhide Asakawa
Targeted gene expression by the Gal4-UAS system is a powerful methodology for analyzing function of genes and cells in vivo and has been extensively used in genetic studies in Drosophila. On the other hand, the Gal4-UAS system had not been applied effectively to vertebrate systems for a long time mainly due to the lack of an efficient transgenesis method. Recently, a highly efficient transgenesis method using the medaka fish Tol2 transposable element was developed in zebrafish. Taking advantage of the Tol2 transposon system, we and other groups developed the Gal4 gene trap and enhancer trap methods and established various transgenic fish expressing Gal4 in specific cells. By crossing such Gal4 lines with transgenic fish lines harboring various reporter genes and effector genes downstream of UAS (upstream activating sequence), specific cells can be visualized and manipulated in vivo by targeted gene expression. Thus, the Gal4 gene trap and enhancer trap approaches together with various UAS lines should be important tools for investigating roles of genes and cells in vertebrates. [source]

Supported Lipid Bilayer on Nanocrystalline Diamond: Dual Optical and Field-Effect Sensor for Membrane Disruption

ADVANCED FUNCTIONAL MATERIALS, Issue 1 2009
Priscilla Kailian Ang
Abstract It is demonstrated that a good biomimetic model lipid membrane with dynamic fluidity can be established on optically transparent nanocrystalline diamond (OTND) with surface roughness below 10,nm. Maigainin II, an antimicrobial peptide, is chosen to investigate the permeation of artificial bacterial membranes constructed on OTND. Due to the unique combination of optical transparency and highly sensitive surface conducting channel, intrinsic OTND affords the possibility of dual-mode sensing based on optical and field effect properties. This opens up new possibilities for making integrated biomolecule,semiconductor microdevices, or sensors where the binding of biomolecules can be tracked using confocal microscopy whilst the associated changes in charge density during membrane perforation can be tracked using the space charge effect in the semiconductor. Such a synergistic approach may provide a powerful methodology for the screening of specific bactericidal activity on biomimetic membrane systems. [source]

Effects of Concentrated Ownership and Owner Management on Small Business Debt Financing,

JOURNAL OF SMALL BUSINESS MANAGEMENT, Issue 4 2007
Zhenyu Wu
Using unique data and a new powerful Monte Carlo-based statistical tool, we examine the effects of concentrated ownership and owner,management (CO-OM) on the creditor,shareholder agency conflicts in small firms. A significant CO-OM effect from the small business owner's view, but insignificant from the commercial lenders' perspective, is found. Special features of informational asymmetry problems in small firms with CO-OM are also highlighted. Theoretical and empirical contributions are made to the small business management and corporate governance literature. Findings obtained from this research have important implications for small business practitioners as well as researchers, and this study can serve as a reference for policymakers and institutional lenders to assist small firms in successfully raising money through debt financing. In addition, a new powerful methodology is introduced to deal with various potential statistical biases and can be further applied to this line of research. [source]

Embedding HPT: Improving police performance by implementing human performance technology in the Royal Canadian Mounted Police

PERFORMANCE IMPROVEMENT, Issue 3 2009
William Pullen
Good police performance enables social capital, strengthens communities, and helps build civil society. Since 2003, Canada's national police force, the Royal Canadian Mounted Police (RCMP), has used human performance technology to improve performance in more than 300 sites across Canada and also as a core part of the RCMP leadership talent pipeline. The lessons learned may be of interest to others contemplating a large-scale distributed use of this powerful methodology. [source]

Current Applications of Microarrays in Head and Neck Cancer Research

THE LARYNGOSCOPE, Issue 2 2004
FRCS Eng, Giles C. Warner MSc
Abstract Objectives/Hypothesis: The objective was to introduce microarray technology and its applications in cancer research to the head and neck clinician. Study Design: Literature review combined with methodology and examples from the authors' experiences with microarray analysis of tumors of the head and neck. Methods: Search of literature and the authors' experience was made for technical details, alternative methods of data analysis, available bioinformatics tools, and applications of microarrays in cancer research. Results: Microarrays allow the simultaneous analysis of the expression of thousands of genes. The use of a well-developed microarray study design leads to informative results. There are various bioinformatics resources widely available to aid in the analysis of microarray data. However, there is not yet a gold standard for analysis because this methodology is still evolving. Conclusion: Microarray studies may allow researchers to identify genetic changes relevant to diagnosis and prognosis in patients with head and neck cancer. Although still relatively new, this powerful methodology has immense potential to aid in understanding of the genetic changes that are important in head and neck cancer. [source]

Engineering Chiral Catalysts through Asymmetric Activation and Super High Throughput Screening (SHTS)

CHINESE JOURNAL OF CHEMISTRY, Issue 6 2001
Koichi Mkami
Abstract A conceptually new strategy for asymmetric catalysis, namely asymmetric activation, in which a chiral activator selectively activates one enantiomer of a racemic chiral catalyst, and a highly efficient screening system for finding the most effective catalysts, namely super high throughput screening (SHTS), by which the reaction can be conducted in parallel and the ee% of the product is allowed to determine within minutes, are summarized in the present account. It is reasonable to believe that SHTS technique combined with asymmetric activation or deactivation principle will provide a very powerful methodology for finding the new catalysts and the best catalyst tuning for asymmetric reactions. [source]