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Pollen Allergic Patients (pollen + allergic_patient)

Distribution by Scientific Domains
Medical Sciences83%

Kinds of Pollen Allergic Patients

  • grass pollen allergic patient
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    Selected Abstracts

    Simultaneous flow cytometric detection of basophil activation marker CD63 and intracellular phosphorylated p38 mitogen-activated protein kinase in birch pollen allergy,

    CYTOMETRY, Issue 1 2009
    Nicolaas E. Aerts
    Abstract Background: Phosphorylation of p38 MAPK is a crucial step in IgE-receptor signaling in basophils. The relation of p38 MAPK to the well-validated diagnostic cell surface marker CD63 has not been evaluated in a clinical allergy model. Methods: Expression of CD63 and phosphorylation of p38 MAPK were analyzed flow cytometrically in anti-IgE-gated basophils from 18 birch pollen allergic patients, five grass pollen allergic patients, and five healthy individuals, after 3 and 20 min of stimulation with recombinant major birch pollen allergen (rBet v 1). Additional time points and the influence of p38 MAPK inhibitor SB203580 were studied in birch pollen allergic patients. Results: Phospho-p38 MAPK and CD63 were expressed dose-dependently in birch pollen allergic patient basophils within 1 minute of rBet v 1 stimulation. P38 MAPK phosphorylation was fastest and subsided gradually while CD63 expression remained elevated for at least 20 min. Inhibition of p38 MAPK significantly inhibited CD63 upregulation. With optimal stimulation of the cells (1 ,g/mL), sensitivity and specificity for the discrimination between patients and a group of control individuals (grass pollen allergic patients and healthy controls) were 94% and 100% for CD63 at 3 and 20 min and for phospho-p38 MAPK at 3 min. Conclusion: Antigen-induced p38 MAPK phosphorylation in human basophils essentially contributes to CD63 upregulation. It is a sensitive and specific intracellular marker for allergy diagnosis and offers new insight into the mechanisms of basophil activation. © 2008 Clinical Cytometry Society [source]

    Time-series nasal epithelial transcriptomics during natural pollen exposure in healthy subjects and allergic patients

    ALLERGY, Issue 2 2010
    P. Mattila
    To cite this article: Mattila P, Renkonen J, Toppila-Salmi S, Parviainen V, Joenväärä S, Alff-Tuomala S, Nicorici D, Renkonen R. Time-series nasal epithelial transcriptomics during natural pollen exposure in healthy subjects and allergic patients. Allergy 2010; 65: 175,183. Abstract Background:, The role of epithelium has recently awakened interest in the studies of type I hypersensitivity. Objective:, We analysed the nasal transcriptomics epithelial response to natural birch pollen exposure in a time series manner. Methods:, Human nasal epithelial cell swabs were collected from birch pollen allergic patients and healthy controls in winter season. In addition, four specimens at weekly intervals were collected from the same subjects during natural birch pollen exposure in spring and transcriptomic analyses were performed. Results:, The nasal epithelium of healthy subjects responded vigorously to allergen exposure. The immune response was a dominating category of this response. Notably, the healthy subjects did not display any clinical symptoms regardless of this response detected by transcriptomic analysis. Concomitantly, the epithelium of allergic subjects responded also, but with a different set of responders. In allergic patients the regulation of dyneins, the molecular motors of intracellular transport dominated. This further supports our previous hypothesis that the birch pollen exposure results in an active uptake of allergen into the epithelium only in allergic subjects but not in healthy controls. Conclusion:, We showed that birch pollen allergen causes a defence response in healthy subjects, but not in allergic subjects. Instead, allergic patients actively transport pollen allergen through the epithelium to tissue mast cells. Our study showed that new hypotheses can arise from the application of discovery driven methodologies. To understand complex multifactorial diseases, such as type I hypersensitivity, this kind of hypotheses might be worth further analyses. [source]

    Micro-arrayed wheat seed and grass pollen allergens for component-resolved diagnosis

    ALLERGY, Issue 7 2009
    C. Constantin
    Background:, Wheat is a potent allergen source and can cause baker's asthma, food and pollen allergy. The aim of the study was to develop an allergen micro-array for differential diagnosis of baker's asthma, wheat-induced food allergy and grass pollen allergy. Methods:, We analysed the immunoglobulin-E reactivity profiles of patients suffering from baker's asthma, wheat-induced food allergy and grass pollen allergy to micro-arrayed recombinant wheat flour allergens and grass pollen allergens and compared these results with clinical results and diagnostic tests based on crude wheat flour, wheat pollen and grass pollen allergen extracts. Results:, We identified recombinant wheat flour allergens, which are specifically recognized by patients suffering from baker's asthma, but not from patients with food allergy to wheat or pollen allergy. rPhl p 1 and rPhl p 5 were identified as marker allergens specific for grass pollen allergy. They can be used to replace grass pollen extracts for allergy diagnosis and to identify grass pollen allergic patients among patients suffering from baker's asthma and wheat-induced food allergy. Profilin was identified as a cross-reactive allergen recognized by patients suffering from baker's asthma, food and pollen allergy. Conclusions:, Our results indicate that it will be possible to design serological tests based on micro-arrayed recombinant wheat seed and grass pollen allergens for the discrimination of baker's asthma, wheat-induced food allergy and grass pollen allergy. [source]

    Specific IgE serum concentration is associated with symptom severity in children with seasonal allergic rhinitis

    ALLERGY, Issue 10 2008
    C. Rolinck-Werninghaus
    Background:, The impact of allergen-specific and total IgE serum levels before and during the pollen season on symptom severity as well as efficacy of treatment with anti-IgE requires further delineation. Methods:, Birch and grass pollen allergic patients aged 6,17 years with seasonal allergic rhinitis (SAR) were analyzed for the association of IgE serum concentration with symptom severity and rescue medication use (combination: symptom load, SL) during the grass pollen season. Reference group A (n = 53) received placebo, while group B (n = 54) received Omalizumab (anti-IgE) monotherapy before and during the grass pollen season. Results:, Patients on placebo with high baseline specific grass pollen IgE (>50 kU/l) had a significantly higher SL compared with those with low IgE levels (,50 kU/l): SL 1.28 vs 0.61, P = 0.015. This association was nonexistent in patients treated with anti-IgE. In contrast, baseline total IgE levels did not correlate with SL in any group. Patients with anti-IgE treatment and high free total IgE levels (>16.7 ng/ml) had a significantly higher SL compared with those with low free total IgE levels (,16.7 ng/ml): SL 0.63 vs 0.23, P = 0.031. Conclusions:, Baseline specific IgE, but not total IgE, is associated with symptom severity during the pollen season in children with SAR. Likewise, the symptom load in SAR patients with anti-IgE correlates with free total IgE levels. Although further research in larger populations is needed to confirm our findings, our data suggest that specific IgE can be used as a parameter for patient selection for this kind of treatment. [source]

    Quantification of group 5 grass pollen allergens in house dust

    CLINICAL & EXPERIMENTAL ALLERGY, Issue 11 2000
    B. Fahlbusch
    Background It is widely known and accepted that grass pollen is a major outdoor cause of hay fever. However, it is of virtual importance for grass pollen allergic patients with symptoms all the year round to know the concentration of grass pollen allergens in their homes. Objective The main objective of this study was to quantify the amount of grass pollen allergen in mass units (,g Phl p 5) in dust settled indoors and to detect the distribution of allergenic activity in different sampling locations of homes. Furthermore, we studied the seasonal fluctuation of allergen content in dust samples. Methods We adapted the two site binding assay for detection of group 5 grass pollen allergens in samples from randomly selected homes in Hamburg (n = 371), Erfurt (n = 396), Hettstedt (n = 353), Zerbst (n = 289) and Bitterfeld (n = 226), Germany. Dust samples were collected from floor of living room (LR), bedroom (BR) or children's room (CR) and mattress (MA) during period of June 1995 to August 1998. The amount of the major grass group 5 allergens was detected in ,g/g dust. Results Phl p 5 was detected in 67% of the samples analysed (n = 4760). The range was between undetectable (< 0.03 ,g/g dust) and 81 ,g/g dust. Phl p 5 levels were significantly higher in the dust from LR (geometric mean 0.117 ,g/g dust) or BR/CR floors (geometric mean 0.098 ,g/g dust) than in mattresses (geometric mean 0.043 ,g/g dust). We observed seasonal fluctuation of indoor Phl p 5 levels with peak in June but also annual differences. Thus Phl p 5 content indoors reflects also the different quantities of pollen counts of annual courses. During pollination period we found two times higher Phl p 5 levels (0.172 ,g/g dust, P < 0.001) than outside of grass pollination season (0.095 ,g/g dust). The indoor Phl p 5 levels outside of season seem to be independent of pollination before. We suppose that settled pollen grains or allergenic material from outdoor particles carried indoors via footwear and clothes accumulates in house dust. Conclusion Although we not known how the allergens in settled dust are equilibrated with those in the air, the considerable high level of Phl p 5 in indoor dust even during periods when no grass pollen is present in the atmosphere may be an important cause of pollen-allergy symptoms outside of season. [source]

    Rationale for a five-grass pollen vaccine

    CLINICAL & EXPERIMENTAL ALLERGY REVIEWS, Issue 1 2008
    P. Moingeon
    Summary Grass pollen allergic patients are naturally exposed and sensitized to multiple pollens from various Pooideae species. The question arises as to whether such patients should be desensitized with extracts based on a single pollen or a pollen mixture. Neither conventional diagnosis based on IgE reactivity nor pollen counts enable the identification of which grass species are involved in patient sensitization. Significant cross-immunogenicity is observed between allergens from Pooideae pollens due to their conserved amino acid sequences (e.g. >90% for group 1, 55,80% for group 5, 30,60% for other allergens, including minor allergens). Nevertheless, pollen allergens also contain species-specific T or B cell epitopes, and there is evidence that at least 50% of allergic patients are sensitized to such distinct epitopes. In addition, substantial quantitative differences exist in the allergen (e.g. group 1, 2/3, 4, 5, 6, 10, 13) content of pollens obtained from distinct grass species. In this context, we recommend a vaccine consisting of a combination of pollens from five common and well characterized Pooideae including Anthoxanthum odoratum, Dactylis glomerata, Lolium perenne, Phleum pratense and Poa pratensis for desensitization purposes. This 5-grass mixture is recommended because, (i) it has been validated, in terms of safety and efficacy, by established clinical practice; (ii) it better reflects natural exposure and sensitization conditions at the molecular level than a single pollen; and (iii) it provides a consistent and well-balanced composition of critical allergens, while extending the repertoire of T and B cell epitopes. [source]