Ash Weight (ash + weight)

Distribution by Scientific Domains

Selected Abstracts

Effects of different pre-freezing blanching procedures on the physicochemical properties of Brassica rapa leaves (Turnip Greens, Grelos)

Alicia del Carmen Mondragón-Portocarrero
Summary For optimal freeze storage, green vegetables should first be blanched. The present study compared four different procedures for the blanching of grelos (leaves of Brassica rapa L.): steaming for 2 min, immersion in boiling water for 2 min, immersion in boiling water containing 1% citric acid for 1 min, and immersion in boiling water containing 5% citric acid for 1 min. After blanching, the grelos were stored for up to 120 days at ,18 °C, with sampling at two-weekly intervals for analysis of physicochemical properties (ash weight, vitamin C content, pH, acid value, moisture content and CIEL*a*b* colour variables). In almost all respects steam blanching gave the best results: notably, vitamin C losses were markedly lower, while moisture content and colour remained closer to those of the fresh product. [source]

RANKL Inhibition with Osteoprotegerin Increases Bone Strength by Improving Cortical and Trabecular bone Architecture in Ovariectomized Rats,,

Michael S Ominsky
Abstract Introduction: Ovariectomy (OVX) results in bone loss caused by increased bone resorption. RANKL is an essential mediator of bone resorption. We examined whether the RANKL inhibitor osteoprotegerin (OPG) would preserve bone volume, density, and strength in OVX rats. Materials and Methods: Rats were OVX or sham-operated at 3 mo of age. Sham controls were treated for 6 wk with vehicle (Veh, PBS). OVX rats were treated with Veh or human OPG-Fc (10 mg/kg, 2/wk). Serum RANKL and TRACP5b was measured by ELISA. BMD of lumbar vertebrae (L1,L5) and distal femur was measured by DXA. Right distal femurs were processed for bone histomorphometry. Left femurs and the fifth lumbar vertebra (L5) were analyzed by ,CT and biomechanical testing, and L6 was analyzed for ash weight. Results: OVX was associated with significantly greater serum RANKL and osteoclast surface and with reduced areal and volumetric BMD. OPG markedly reduced osteoclast surface and serum TRACP5b while completely preventing OVX-associated bone loss in the lumbar vertebrae, distal femur, and femur neck. Vertebrae from OPG-treated rats had increased dry and ash weight, with no significant differences in tissue mineralization versus OVX controls. ,CT showed that trabecular compartments in OVX-OPG rats had significantly greater bone volume fraction, vBMD, bone area, trabecular thickness, and number, whereas their cortical compartments had significantly greater bone area (p < 0.05 versus OVX-Veh). OPG improved cortical area in L5 and the femur neck to levels that were significantly greater than OVX or sham controls (p < 0.05). Biomechanical testing of L5 and femur necks showed significantly greater maximum load values in the OVX-OPG group (p < 0.05 versus OVX-Veh). Bone strength at both sites was linearly correlated with total bone area (r2 = 0.54,0.74, p < 0.0001), which was also significantly increased by OPG (p < 0.05 versus OVX). Conclusions: OPG treatment prevented bone loss, preserved trabecular architecture, and increased cortical area and bone strength in OVX rats. [source]

Estimating the Timing of Long Bone Fractures: Correlation Between the Postmortem Interval, Bone Moisture Content, and Blunt Force Trauma Fracture Characteristics,

Danielle A.M. Wieberg M.A.
Abstract:, There is very limited knowledge about how long perimortem fracture characteristics persist into the postmortem interval (PMI). Therefore, in this study, 60 porcine long bones were exposed to natural taphonomic conditions and fractured with a steel bone breaking apparatus every 28 days throughout a 141-day period. Differences between macroscopic blunt force trauma fracture characteristics (fracture angle, surface morphology, and outline) were examined to determine if they varied over time or in relationship to bone moisture content (ash weight) and overall assessment. There are significant relationships between (1) PMI and percent ash weight (%AW), fracture surface, and fracture angle and (2) %AW and fracture surface and fracture angle. Bone moisture content correlates significantly with fracture morphology and other characteristics commonly used by forensic anthropologists to determine the timing of traumatic injuries. However, fracture characteristics normally associated with perimortem trauma can persist long into the PMI. [source]


Naomi Nishii
SUMMARY 1High-blood pressure or diabetes may be related to the loss of bone mass or the development of osteoporosis. We examined the mechanical bone properties of the SHR/NDmcr-cp (SHR-cp) rat, an obese strain that develops hypertension, hyperlipidaemia and insulin-independent diabetes. 2The mechanical properties of the femur of 22-week-old Wistar-Kyoto (WKY) and SHR-cp rats were measured by Peng's three-point bending procedure modified by Shintani. Femurs were then defatted and dried. After weighing, the dried bones were ashed and the ash was weighed. The values of the dry weight, ash weight and ash weight/dry weight (%) were used as a description of the physical parameters of the bone. 3All values of stiffness, strength, toughness and ductility in SHR-cp were significantly lower than those of WKY rats (P < 0.05). The value of ash weight/dry weight (%) was lower in SHR-cp rats (P < 0.01). These results showed that bone fragility was greater in SHR-cp rats, indicative of osteopenia. [source]