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PCL Scaffolds (pcl + scaffold)

Distribution by Scientific Domains

Polymers and Materials Science	50%
Medical Sciences	50%

Selected Abstracts

Dual-Growth-Factor-Releasing PCL Scaffolds for Chondrogenesis of Adipose-Tissue-Derived Mesenchymal Stem Cells,

ADVANCED ENGINEERING MATERIALS, Issue 1-2 2010
Sung Mook Lim
Polycaprolactone/Pluronic F127 porous scaffolds are prepared using a modified melt-molding particulate-leaching method. The scaffolds are highly porous (about 90% porosity) and have open-cellular pore structures. Growth factors (TGF- ,2, BMP-7 or dual TGF- ,2/BMP-7) can be easily immobilized on the pore surfaces of the PCL/F127 scaffolds via binding with heparin. The growth-factor-immobilized scaffolds can induce the chondrogenesis of ATMSCs seeded onto them. Using TGF-,2 and BMP-7 growth factors together leads to a better chondrogenic differentiation behavior than using single-growth-factor immobilized scaffolds. [source]

Bladder wall grafting in rats using salt-modified and collagen-coated polycaprolactone scaffolds: Preliminary report

INTERNATIONAL JOURNAL OF UROLOGY, Issue 10 2007
Dah-Shyong Yu
Aim: A rat model was used for the evaluation of collagen-coated and salt-modified polycaprolactone (PCL) scaffolds for bladder grafting after hemicystectomy. Methods: SD rats underwent partial cystectomy and cystoplasty with collagen-coated and salt-modified polycaprolactone scaffolds. The grafts of the regenerated bladder wall were harvested at different intervals and tissue regeneration was evaluated microscopically. Anatomic and functional characters were evaluated by cystography and urodynamics. Results: At harvesting, after 1 and 2 months, we found good preservation of the bladder shape and volume in all 16 rats receiving PCL cystorrhaphy. No stone formation was observed. Good epithelialization and ingrowth of smooth muscle cells were seen after 2 months grafting. Collagen-coated PCL scaffolds showed considerable encrustation, which appeared to be absorbed and disappear with time. The cystographic and urodynamic examinations revealed intact contour and a well-accommodated bladder with reservoir volume and contractility. Conclusions: In the rat model, we have successfully demonstrated the applicability of collagen coated and salt-modified PCL in reconstruction of the partial cystectomized bladder. [source]

Evaluation of articular cartilage repair using biodegradable nanofibrous scaffolds in a swine model: a pilot study

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, Issue 1 2009
Wan-Ju Li
Abstract The aim of this study was to evaluate a cell-seeded nanofibrous scaffold for cartilage repair in vivo. We used a biodegradable poly(,-caprolactone) (PCL) nanofibrous scaffold seeded with allogeneic chondrocytes or xenogeneic human mesenchymal stem cells (MSCs), or acellular PCL scaffolds, with no implant as a control to repair iatrogenic, 7 mm full-thickness cartilage defects in a swine model. Six months after implantation, MSC-seeded constructs showed the most complete repair in the defects compared to other groups. Macroscopically, the MSC-seeded constructs regenerated hyaline cartilage-like tissue and restored a smooth cartilage surface, while the chondrocyte-seeded constructs produced mostly fibrocartilage-like tissue with a discontinuous superficial cartilage contour. Incomplete repair containing fibrocartilage or fibrous tissue was found in the acellular constructs and the no-implant control group. Quantitative histological evaluation showed overall higher scores for the chondrocyte- and MSC-seeded constructs than the acellular construct and the no-implant groups. Mechanical testing showed the highest equilibrium compressive stress of 1.5 MPa in the regenerated cartilage produced by the MSC-seeded constructs, compared to 1.2 MPa in the chondrocyte-seeded constructs, 1.0 MPa in the acellular constructs and 0.2 MPa in the no-implant group. No evidence of immune reaction to the allogeneically- and xenogeneically-derived regenerated cartilage was observed, possibly related to the immunosuppressive activities of MSCs, suggesting the feasibility of allogeneic or xenogeneic transplantation of MSCs for cell-based therapy. Taken together, our results showed that biodegradable nanofibrous scaffolds seeded with MSCs effectively repair cartilage defects in vivo, and that the current approach is promising for cartilage repair. Copyright © 2008 John Wiley & Sons, Ltd. [source]

Development of Electrospun Three-arm Star Poly(, -caprolactone) Meshes for Tissue Engineering Applications

MACROMOLECULAR BIOSCIENCE, Issue 8 2010
Dario Puppi
Abstract We have developed three-dimensional electrospun microfibrous meshes of a novel star branched three-arm poly(, -caprolactone) (*PCL) as potential scaffolds for tissue engineering applications. The processing conditions required to obtain uniform fibers were optimized by studying their influence on fiber morphology and size. Polymer molecular weight and solution feed rate influenced both the mesh microstructure and the tensile properties of the developed mats. Electrospun samples were also tested for their mechanical properties in wet conditions, showing higher yield strength and strain in comparison to that observed in dry conditions. Cell culture experiments employing MC3T3-E1 osteoblast like cells showed good cell viability adhesion and collagen production on the *PCL scaffolds. [source]