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Past Several Years (past + several_year)
Selected AbstractsBETTER GUN ENFORCEMENT, LESS CRIME,CRIMINOLOGY AND PUBLIC POLICY, Issue 4 2005JENS LUDWIG Research Summary: Project Safe Neighborhoods (PSN), which for the past several years has been the major federal initiative to combat gun violence, includes several elements (such as gun locks and other efforts to reduce gun availability) that research suggests are likely to have at best modest effects on gun crime. In general, enforcement activities targeted at the "demand side" of the underground gun market currently enjoy stronger empirical support. However much of PSN's budget has been devoted to increasing the severity of punishment, such as by federaliz-ing gun cases, which seems to be less effective than targeted street-level enforcement designed to increase the probability of punishment for gun carrying or use in crime. Policy Implications: PSN and other enforcement activities could be made more effective by redirecting resources toward activities such as targeted patrols against illegal gun carrying. Given the substantial social costs of gun violence, an efficiency argument can also be made for increasing funding beyond previous levels. [source] Genetics of anxiety disorders: the complex road from DSM to DNA,DEPRESSION AND ANXIETY, Issue 11 2009Jordan W. Smoller M.D. Sc.D. Abstract Anxiety disorders are among the most common psychiatric disorders, affecting one in four individuals over a lifetime. Although our understanding of the etiology of these disorders is incomplete, familial and genetic factors are established risk factors. However, identifying the specific casual genes has been difficult. Within the past several years, advances in molecular and statistical genetic methods have made the genetic dissection of complex disorders a feasible project. Here we provide an overview of these developments, with a focus on their implications for genetic studies of anxiety disorders. Although the genetic and phenotypic complexity of the anxiety disorders present formidable challenges, advances in neuroimaging and experimental animal models of anxiety and fear offer important opportunities for discovery. Real progress in identifying the genetic basis of anxiety disorders will require integrative approaches that make use of these biologic tools as well as larger-scale genomic studies. If successful, such efforts may yield novel and more effective approaches for the prevention and treatment of these common and costly disorders. Depression and Anxiety, 2009. © 2009 Wiley-Liss, Inc. [source] 5-Aminolevulinic Acid Photodynamic Therapy: Where We Have Been and Where We Are GoingDERMATOLOGIC SURGERY, Issue 8 2004Michael H. Gold MD Background. Photodynamic therapy, utilizing the topical administration of 20% 5-aminolevulinic acid, has generated a great deal of interest in the dermatology community over the past several years. Objective. The purpose of this article is to review the history of photodynamic therapy in dermatology and to review recent new advances with this technology that will increase its appeal to all dermatologists. Methods. A literature review and results of new clinical trials with regards to photorejuvenation and acne vulgaris treatments with 5-aminolevulinic acid photodynamic therapy are presented. Results. Short-contact, full-face 5-aminolevulinic acid photodynamic therapy treatments with a variety of lasers and light sources have shown to be successful in treating all facets of photorejuvenation and the associated actinic keratoses as well as disorders of sebaceous glands, including acne vulgaris. The treatments are relatively pain-free, efficacious, and safe. They are also making already available laser/light source therapies work better for acne vulgaris and photorejuvenation. Conclusions. The use of 5-aminolevulinic acid photodynamic therapy with short-contact, full-face broad-application therapy is now able to bridge the world of medical and cosmetic dermatologic surgery. This therapy is available for all dermatologists to utilize in the care of their patients. [source] Improvement of Dermatochalasis and Periorbital Rhytides With a High-Energy Pulsed CO2 Laser: A Retrospective StudyDERMATOLOGIC SURGERY, Issue 4 2004Tina S. Alster MD Background. Upper eyelid dermatochalasis is typically treated with excisional blepharoplasty. The role of the CO2 laser previously had been confined to that of a vaporizing, incisional, or hemostatic tool. Over the past several years, however, ablative CO2 laser skin resurfacing has been popularized as an adjunctive treatment to blepharoplasty to minimize periorbital rhytides through its vaporizing as well as skin-tightening action. Objective. To evaluate the safety and efficacy of a high-energy pulsed CO2 laser as a stand-alone treatment for dermatochalasis and periorbital rhytides. Methods. Sixty-seven patients (skin phototypes I,IV) with mild-to-severe upper eyelid dermatochalasis and periorbital rhytides received periocular CO2 laser skin treatment. Global assessment scores of dermatochalasis and rhytides were determined by a side-by-side comparison of periocular photographs preoperatively and 1, 3, and 6 months postoperatively. In addition, caliper measurements of upper eyelids before and 1, 3, and 6 months after treatment were obtained. Results. Both dermatochalasis and periorbital rhytides were significantly improved after periocular CO2 laser skin resurfacing. Patients with more severe dermatochalasis and rhytides showed greater improvement after CO2 laser treatment than did those with mild or moderate involvement. Side effects were limited to erythema and transient hyperpigmentation. No scarring, hypopigmentation, or ectropion were observed. Conclusions. Periocular skin resurfacing with a CO2 laser can safely and effectively improve upper eyelid dermatochalasis and periorbital rhytides. [source] Powered Liposuction: An Evaluation of Currently Available InstrumentationDERMATOLOGIC SURGERY, Issue 5 2002Timothy Corcoran Flynn MD background. Over the past several years, powered liposuction instruments have become available to the dermatologic surgeon. These instruments all move the cannula forward and backward 2,12 mm with rapidity. This motion simulates the standard human movement of the cannula and facilitates movement through the tissue. Powered liposuction may help to prevent surgeon fatigue and has recently been shown to aid in the efficacy of fat removal. objective. To compare and contrast currently available powered liposuction instruments, to obtain objective engineering data on the instruments, and to compare the instruments when used on actual liposuction cases. methods. Five currently available powered liposuction systems were evaluated by the author. Each instrument was used with a 3 mm dual side port cannula on patients undergoing tumescent liposuction. An independent engineering firm, who collected objective measurements such as weight, stroke force, temperature, and vibration measurements on the instruments, evaluated the units. Noise measurements were obtained. results. Each of the five systems showed advantages and disadvantages. Important issues were the size and weight of the units, as well as whether they were driven electrically or pneumatically. Stroke force is variable (range 9.5,30 pounds). The noise of the units ranged between 60 and 87 dB. Vibration measurements varied from instrument to instrument. Units produced a variable amount of heat ranging from 77°F to 102°F. Most instruments are autoclavable, and one system is disposable. Build quality and reliability varied with the instrument. conclusion. Instruments for powered liposuction have emerged in the last 2 years, with each instrument having distinct advantages and disadvantages. This article reviews five currently available powered liposuction instruments and presents objective measurements as well as the author's experience using the instruments. [source] Comparison of Long-Pulsed Diode and Long-Pulsed Alexandrite Lasers for Hair Removal: A Long-Term Clinical and Histologic StudyDERMATOLOGIC SURGERY, Issue 7 2001Christiane Handrick MD Background. Unwanted facial and body hair is a common problem, generating a high level of interest for treatment innovations. Advances in laser technology over the past several years has led to the development and distribution of numerous red and infrared lasers and light sources to address this issue. Despite the impressive clinical results that have been reported with the use of individual laser hair removal systems, long-term comparative studies have been scarce. Objective. To compare the clinical and histologic efficacy, side effect profile, and long-term hair reduction of long-pulsed diode and long-pulsed alexandrite laser systems. Methods. Twenty women with Fitzpatrick skin types I,IV and dark terminal hair underwent three monthly laser-assisted hair removal sessions with a long-pulsed alexandrite laser (755 nm, 2-msec pulse, 10 mm spot) and a long-pulsed diode laser (800 nm, 12.5 msec or 25 msec, 9 mm spot). Axillary areas were randomly assigned to receive treatment using each laser system at either 25 J/cm2 or 40 J/cm2. Follow-up manual hair counts and photographs of each area were obtained at each of the three treatment visits and at 1, 3, and 6 months after the final laser session. Histologic specimens were obtained at baseline, immediately after the initial laser treatment, and 1 and 6 months after the third treatment session. Results. After each laser treatment, hair counts were successively reduced and few patients found it necessary to shave the sparsely regrown hair. Optimal clinical response was achieved 1 month after the second laser treatment, regardless of the laser system or fluence used. Six months after the third and final treatment, prolonged clinical hair reduction was observed with no significant differences between the laser systems and fluences used. Histologic tissue changes supported the clinical responses observed with evidence of initial follicular injury followed by slow follicular regeneration. Side effects, including treatment pain and vesiculation, were rare after treatment with either laser system, but were observed more frequently with the long-pulsed diode system at the higher fluence of 40 J/cm2. Conclusion. Equivalent clinical and histologic responses were observed using a long-pulsed alexandrite and a long-pulsed diode laser for hair removal with minimal adverse sequelae. While long-term hair reduction can be obtained in most patients after a series of laser treatments, partial hair regrowth is typical within 6 months, suggesting the need for additional treatments to improve the rate of permanent hair removal. [source] Therapy of other viral infections: herpes to hepatitisDERMATOLOGIC THERAPY, Issue 6 2004Arun Chakrabarty ABSTRACT:, Over the past several years, there has been an increase in knowledge pertaining to the diagnosis and management strategies for the herpes family (Types 1,8), the pox viruses, mumps, measles, rubella, and parvovirus B19 as well as the viral etiologies of hepatitis. Various antiviral treatments, such as nucleoside analogs and interferon therapy, have been available to reduce the signs and symptoms of these common viral infections. This article summarizes the preferred treatment strategies to be employed for each of the viruses for reducing severity, duration, recurrences (notably in the herpes family), transmission rates, as well as preventive alternatives. The majority of the therapeutic options attenuate the course of disease. Treatment decisions are driven by knowledge of the natural history and often are tailored to incorporate clinical circumstances for individual patients. Promotion of community awareness and the development of vaccines should be emphasized in the battle against these common viruses, particularly the herpes simplex viruses, the pox viruses, and hepatitis B. [source] Cutaneous infections in the elderly: diagnosis and managementDERMATOLOGIC THERAPY, Issue 3 2003Jeffrey M. Weinberg ABSTRACT:, Over the past several years there have been many advances in the diagnosis and treatment of cutaneous infectious diseases. This review focuses on the three major topics of interest in the geriatric population: herpes zoster and postherpetic neuralgia (PHN), onychomycosis, and recent advances in antibacterial therapy. Herpes zoster in adults is caused by reactivation of the varicella-zoster virus (VZV) that causes chickenpox in children. For many years acyclovir was the gold standard of antiviral therapy for the treatment of patients with herpes zoster. Famciclovir and valacyclovir, newer antivirals for herpes zoster, offer less frequent dosing. PHN refers to pain lasting ,2 months after an acute attack of herpes zoster. The pain may be constant or intermittent and may occur spontaneously or be caused by seemingly innocuous stimuli such as a light touch. Treatment of established PHN through pharmacologic and nonpharmacologic therapy will be discussed. In addition, therapeutic strategies to prevent PHN will be reviewed. These include the use of oral corticosteroids, nerve blocks, and treatment with standard antiviral therapy. Onychomycosis, or tinea unguium, is caused by dermatophytes in the majority of cases, but can also be caused by Candida and nondermatophyte molds. Onychomycosis is found more frequently in the elderly and in more males than females. There are four types of onychomycosis: distal subungual onychomycosis, proximal subungual onychomycosis, white superficial onychomycosis, and candidal onychomycosis. Over the past several years, new treatments for this disorder have emerged which offer shorter courses of therapy and greater efficacy than previous therapies. The treatment of bacterial skin and skin structure infections in the elderly is an important issue. There has been an alarming increase in the incidence of gram-positive infections, including resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) and drug-resistant pneumococci. While vancomycin has been considered the drug of last defense against gram-positive multidrug-resistant bacteria, the late 1980s saw an increase in vancomycin-resistant bacteria, including vancomycin-resistant enterococci (VRE). More recently, strains of vancomycin-intermediate resistant S. aureus (VISA) have been isolated. Gram-positive bacteria, such as S. aureus and Streptococcus pyogenes are often the cause of skin and skin structure infections, ranging from mild pyodermas to complicated infections including postsurgical wound infections, severe carbunculosis, and erysipelas. With limited treatment options, it has become critical to identify antibiotics with novel mechanisms of activity. Several new drugs have emerged as possible therapeutic alternatives, including linezolid and quinupristin/dalfopristin. [source] The Blood,Brain Barrier and EpilepsyEPILEPSIA, Issue 11 2006Emily Oby Summary:, During the past several years, there has been increasing interest in the role of the blood,brain barrier (BBB) in epilepsy. Advances in neuroradiology have enhanced our ability to image and study the human cerebrovasculature, and further developments in the research of metabolic deficiencies linked to seizure disorders (e.g., GLUT1 deficiency), neuroinflammation, and multiple drug resistance to antiepileptic drugs (AEDs) have amplified the significance of the BBB's relationship to epilepsy. Prior to 1986, BBB research in epilepsy focused on three main areas: ultrastructural studies, brain glucose availability and transport, and clinical uses of AEDs. However, contrast-based imaging techniques and medical procedures such as BBB disruption provided a framework that demonstrated that the BBB could be reversibly disrupted by pathologic or iatrogenic manipulations, with important implications in terms of CNS drug delivery to "multiple drug resistant" brain. This concept of BBB breakdown for therapeutic purposes has also unveiled a previously unrecognized role for BBB failure as a possible etiologic mechanism in epileptogenesis. Finally, a growing body of evidence has shown that inflammatory mechanisms may participate in the pathological changes observed in epileptic brain, with increasing awareness that blood-borne cells or signals may participate in epileptogenesis by virtue of a leaky BBB. In this article we will review the relationships between BBB function and epilepsy. In particular, we will illustrate consensus and divergence between clinical reality and animal studies. [source] A methodology for simulating power system vulnerability to cascading failures in the steady stateEUROPEAN TRANSACTIONS ON ELECTRICAL POWER, Issue 8 2008Murali Kumbale Abstract Simulations of power system conditions and event sequences leading to either local or widespread blackout has acquired increasing importance following wide-impact network collapses that have occurred over the past several years in North America and Europe. This paper summarizes an analytical framework that has been developed, implemented, and practically used by Southern Company to evaluate system vulnerability to cascading failures using the steady state model. This methodology was first used by Southern Company in 1999. The studies performed at Southern Company have already influenced and motivated certain transmission development projects. Future improvements to the method could include better modeling and sequencing of cascading steps, including time sequence of failures using equipment response time. Significant interest exists in developing preventive methods and procedures and in the application of this technology in the infrastructure security arena. Copyright © 2008 John Wiley & Sons, Ltd. [source] Contemporary Models of Youth Development and Problem Prevention: Toward an Integration of Terms, Concepts, and ModelsFAMILY RELATIONS, Issue 1 2004Stephen Small Over the past several years, increased interest in preventing youth problems and promoting healthy youth development has led youth and family practitioners, policy makers, and researchers to develop a wide range of approaches based on various theoretical frameworks. Although the growth in guiding frameworks has led to more complex models and a greater diversity in the options available to scholars and practitioners, the lack of an integrative conceptual scheme and consistent terminology has led to some confusion in the field. Here, we provide an overview of three approaches to youth development and problem prevention, critically examine their strengths and weaknesses, and offer some elaborations to help clarify, extend, and integrate the models. We conclude by discussing some general implications for researchers, practitioners, and policy makers. [source] Hydrogen Storage in Metal,Organic FrameworksADVANCED MATERIALS, Issue 20 2010Yun Hang Hu Abstract Metal,organic frameworks (MOFs) are highly attractive materials because of their ultra-high surface areas, simple preparation approaches, designable structures, and potential applications. In the past several years, MOFs have attracted worldwide attention in the area of hydrogen energy, particularly for hydrogen storage. In this review, the recent progress of hydrogen storage in MOFs is presented. The relationships between hydrogen capacities and structures of MOFs are evaluated, with emphasis on the roles of surface area and pore size. The interaction mechanism between H2 and MOFs is discussed. The challenges to obtain a high hydrogen capacity at ambient temperature are explored. [source] Is there a link between adult neurogenesis and learning?HIPPOCAMPUS, Issue 3 2006Benedetta Leuner Abstract During the past several years, evidence has accumulated suggesting a relationship between newly born cells in the hippocampus and various types of learning and memory. However, most of the evidence is correlational and some of it does not agree. This review discusses both sides of this issue, considering the effects of learning on the production of new neurons in the dentate gyrus and the question of whether newly born cells participate in learning and memory. © 2006 Wiley-Liss, Inc. [source] The effects of organizational learning culture and job satisfaction on motivation to transfer learning and turnover intentionHUMAN RESOURCE DEVELOPMENT QUARTERLY, Issue 3 2004Toby Marshall Egan Although organizational learning theory and practice have been clarified by practitioners and scholars over the past several years, there is much to be explored regarding interactions between organizational learning culture and employee learning and performance outcomes. This study examined the relationship of organizational learning culture, job satisfaction, and organizational outcome variables with a sample of information technology (IT) employees in the United States. It found that learning organizational culture is associated with IT employee job satisfaction and motivation to transfer learning. Turnover intention was found to be negatively influenced by organizational learning culture and job satisfaction. Suggestions for future study of learning organizational culture in association with job satisfaction and performance-related outcomes are discussed. [source] Robust design of communication systems: The case of expedited forwarding of voice traffic in differentiated services networksINTERNATIONAL JOURNAL OF COMMUNICATION SYSTEMS, Issue 10 2007Hyoup-Sang Yoon Abstract Design of experiments (DOE) is gaining acceptance in the community of telecommunication researchers, especially during the past several years. In this paper, a state-of-the-art review on the use of DOE in the field of communication networks is presented, and the need for introducing a systematic robust design methodology to network simulations or testbed experiments is identified in ensuring robust behaviours of a network against uncontrollable sources of variation. Then, the Taguchi robust design methodology is applied for optimizing the expedited forwarding (EF) of voice traffic in a differentiated services network, and its step-by-step procedures are described in detail. The experimental data are collected using the ns-2 simulator, and the SN ratio, a robustness measure, is analysed to determine an optimal design condition for each performance characteristic. The analysis results show that ,type of queue scheduling schemes' is a major control factor for ensuring robust behaviours of one-way delay and jitter while ,EF queue size' is for throughput and loss rate. Finally, a compromised optimal design condition is identified using a desirability function approach adapted to multi-characteristic robust design problems. Copyright © 2007 John Wiley & Sons, Ltd. [source] Mechanism of light-induced translocation of arrestin and transducin in photoreceptors: Interaction-restricted diffusionIUBMB LIFE, Issue 1 2008Vladlen Z. Slepak Abstract Many signaling proteins change their location within cells in response to external stimuli. In photoreceptors, this phenomenon is remarkably robust. The G protein of rod photoreceptors and rod transducin concentrates in the outer segments (OS) of these neurons in darkness. Within ,30 minutes after illumination, rod transducin redistributes throughout all of the outer and inner compartments of the cell. Visual arrestin concurrently relocalises from the inner compartments to become sequestered primarily within the OS. In the past several years, the question of whether these proteins are actively moved by molecular motors or whether they are redistributed by simple diffusion has been extensively debated. This review focuses on the most essential works in the area and concludes that the basic principle driving this protein movement is diffusion. The directionality and light dependence of this movement is achieved by the interactions of arrestin and transducin with their spatially restricted binding partners. © 2007 IUBMB IUBMB Life, 60(1): 2,9, 2008 [source] A Chemical Approach Towards Understanding the Mechanism and Reversal of Drug Resistance in Plasmodium falciparum: Is it Viable?IUBMB LIFE, Issue 4-5 2002Kelly Chibale Abstract Genetic and biochemical approaches to studies of drug resistance mechanisms in Plasmodium falciparum have raised controversies and contradictions over the past several years. A different and novel chemical approach to this important problem is desirable at this point in time. Recently, the molecular basis of drug resistance in P. falciparum has been associated with mutations in the resistance genes, Chloroquine Resistance Transporter (PfCRT) and the P-glycoprotein homologue (Pgh1). Although not the determinant of chloroquine resistance in P. falciparum, mutations in Pgh1 have important implications for resistance to other antimalarial drugs. Because it is mutations in the aforementioned resistance genes rather than overexpression that has been associated with drug resistance in malaria, studies on mechanisms of drug resistance and its reversal by chemosensitisers should benefit from a chemical approach. Target-oriented organic synthesis of chemosensitisers against proteins implicated in drug resistance in malaria should shed light on mechanism of drug resistance and its reversal in this area. The effect of structurally diverse chemosensitisers should be examined on several putative resistance genes in P. falciparum to deal with antimalarial drug resistance in the broadest sense. Therefore, generating random mutations of these resistance proteins and subsequent screening in search of a specific phenotype followed by a search for mutations and/or chemosensitisers that affect a specific drug resistance pathway might be a viable strategy. This diversity-oriented organic synthesis approach should offer the means to simultaneously identify resistance proteins that can serve as targets for therapeutic intervention (therapeutic target validation) and chemosensitisers that modulate the functions of these proteins (chemical target validation). [source] Glial connexins and gap junctions in CNS inflammation and diseaseJOURNAL OF NEUROCHEMISTRY, Issue 3 2008Tammy Kielian Abstract Gap junctions facilitate direct cytoplasmic communication between neighboring cells, facilitating the transfer of small molecular weight molecules involved in cell signaling and metabolism. Gap junction channels are formed by the joining of two hemichannels from adjacent cells, each composed of six oligomeric protein subunits called connexins. Of paramount importance to CNS homeostasis are astrocyte networks formed by gap junctions, which play a critical role in maintaining the homeostatic regulation of extracellular pH, K+, and glutamate levels. Inflammation is a hallmark of several diseases afflicting the CNS. Within the past several years, the number of publications reporting effects of cytokines and pathogenic stimuli on glial gap junction communication has increased dramatically. The purpose of this review is to discuss recent observations characterizing the consequences of inflammatory stimuli on homocellular gap junction coupling in astrocytes and microglia as well as changes in connexin expression during various CNS inflammatory conditions. [source] Evaluating the Performance of Third-Party Logistics Arrangements: A Relationship Marketing PerspectiveJOURNAL OF SUPPLY CHAIN MANAGEMENT, Issue 1 2004A. Michael Knemeyer SUMMARY By 2005, users of third-party logistics services may be spending an average of nearly one-third of their total logistics budgets (compared to 20 percent today) to support 3PL services (Gooley 2000). Yet, very little research has examined managerial activities that might influence the performance of these logistics outsourcing relationships. Over the past several years, the management approach that views relationships as key assets of the organization has gained increased prominence in the priorities and practices of many companies (Gruen, Summers and Acito 2000). The current study utilizes this relationship marketing perspective as the basis for evaluating the perceived performance of third-party logistics arrangements. In particular, the current study examines the influence of six key relationship marketing dimensions on a customer's perceptions of their 3PL provider's performance. In so doing, the article builds on research (e.g., Goldsby and Stank 2000) that focuses on potential linkages between logistical performance metrics and managerial activities. The results suggest linkages between relationship marketing activities and the perceived performance of the 3PL arrangement. [source] USING MARYLAND'S STREAM CORRIDOR ASSESSMENT SURVEY TO PRIORITIZE WATERSHED RESTORATION EFFORTS,JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION, Issue 4 2002Kenneth T. Yetman ABSTRACT: The Stream Corridor Assessment survey has been developed by the Maryland Department of Natural Resources as a watershed management tool to identify environmental problems and to help prioritize restoration opportunities on a watershed basis. Potential environmental problems commonly identified during the survey include: stream channel alterations, excessive bank erosion, exposed pipes, inadequate stream buffers, fish migration blockages, trash dumping sites, near stream construction, pipe outfalls, and unusual conditions. In addition, the survey records information on the location of potential wetlands creation sites and collects data on the general condition of instream and riparian habitats. Over the past several years, in collaboration with the Maryland Conservation Corps, watershed associations, and local governments, more than 3,293 km (2,046 miles) of Maryland streams have been surveyed. Overall, the survey has proven to be a cost effective starting point for many watershed restoration efforts. [source] Public Safety Outcomes of Virginia's Detention Assessment InstrumentJUVENILE AND FAMILY COURT JOURNAL, Issue 3 2007SCOTT REINER ABSTRACT The placement of juveniles in secure detention has been the subject of considerable attention recently. For the past several years, Virginia has been engaged in efforts to improve detention practices at both the state and local levels. One important policy and practice change is the implementation of a standardized, structured decision-making instrument to guide detention decisions by intake officers of the Virginia Department of Juvenile Justice (DJJ). This report will present a brief background on the Detention Assessment Instrument (DAI); an analysis of the performance of the DAI on key public safety outcomes; and an overview of Virginia's participation in the Juvenile Detention Alternatives Initiative (JDAI). [source] Discussion of laser-assisted liposuction,LASERS IN SURGERY AND MEDICINE, Issue 10 2009Brian D. Zelickson MD Abstract There has been a significant advancement in the use of lasers for body contouring over the past several years. This article will review the recent past and discuss the future of this trend. Introduction There is much confusion over the definition of procedures using lasers for lipolysis and liposuction. This review article discusses the definitions of suction-assisted liposuction, laser lipolysis, and laser-assisted liposuction in detail. The development of these procedures is then discussed and pertinent articles are reviewed. Materials and Methods Pubmed and FDA.gov web sites were searched for published articles and FDA approved devices employing lasers for targeting fat for body contouring. This information along with the authors' personal experience was used to review and discuss this topic. Discussion There is a specific difference between suction-assisted liposuction, laser-assisted liposuction, and laser lipolysis. This review article clarifies these definitions and highlights the recent articles employing lasers for minimally invasive fat reduction. Lasers Surg. Med. 41:709,713, 2009. © 2009 Wiley-Liss, Inc. [source] CSRP2, TIMP-1, and SM22, promoter fragments direct hepatic stellate cell-specific transgene expression in vitro, but not in vivoLIVER INTERNATIONAL, Issue 1 2004Jens Herrmann Abstract: Background/Aims: The activation of hepatic stellate cells (HSC) and their transdifferentiation into myofibroblasts (MFB) is the key step for development of liver fibrosis. Over the past several years, significant progress has been made in the understanding of the critical pathways involved incells undergoing activation. Cellular activation in the course of transdifferentiation involves, among other biochemical modifications, functionally relevant changes in the control of gene expression. These include the up-regulation of transcription factors, different extracellular matrix proteins, cell adhesion molecules, smooth muscle specific genes, and proteins involved in matrix remodelling, or cytoskeletal organization. The corresponding regulatory elements of these genes have afforded us the opportunity to express transgenes with antifibrotic potential in a cell type- and/or transdifferentiation-dependent manner. Methods: In the present study, we have tested several promoters for their ability to mediate cell-specific expression, including those for CSRP2, SM22,, and TIMP-1 (CSRP2, gene encoding the LIM domain protein CRP2; SM22,, smooth muscle-specific gene encoding a 22-kDa protein; TIMP-1, gene encoding the tissue inhibitor of metalloproteinases-1), which in liver are specifically expressed in HSC or become strongly activated during the acute remodelling into MFB. We constructed adenoviral reporter vectors in which relevant portions of the promoters were fused to the green fluorescent protein. Results and Conclusion: Our experiments demonstrate that each of these promoters is sufficient to achieve strong or partially selective expression in vitro but none is able to direct a specific or inducible expression of transgenes in HSC/MFB in vivo. [source] The dynamic microbe: green fluorescent protein brings bacteria to lightMOLECULAR MICROBIOLOGY, Issue 5 2002Carolyn M. Southward Summary The demonstration that the green fluorescent protein (GFP) from the jellyfish Aequorea victoria required no jellyfish-specific cofactors and could be expressed as a fluorescent protein in heterologous hosts including both prokaryotes and eukaryotes sparked the development of GFP as one of the most common reporters in use today. Over the past several years, the utility of GFP as a reporter has been optimized through the isolation and engineering of variants with increased folding rates, different in vivo stabilities and colour variants with altered excitation and emission spectral properties. One of the great utilities of GFP is as a probe for characterizing spatial and temporal dynamics of gene expression, protein localization and protein,protein interactions in living cells. The innovative application of GFP as a reporter in bacteria has made a significant contribution to microbial cell biology. This review will highlight recent studies that demonstrate the potential of GFP for real-time analysis of gene expression, protein localization and the dynamics of signalling transduction pathways through protein,protein interactions. [source] Characterization of Bet v 1-related allergens from kiwifruit relevant for patients with combined kiwifruit and birch pollen allergyMOLECULAR NUTRITION & FOOD RESEARCH (FORMERLY NAHRUNG/FOOD), Issue S2 2008Christina Oberhuber Abstract Allergy to kiwifruit appears to have become more common in Europe and elsewhere during the past several years. Seven allergens have been identified from kiwifruit so far, with actinidin, kiwellin and the thaumatin-like protein as the most relevant ones. In contrast to other fruits, no Bet v 1 homologues were characterized from kiwifruit so far. We cloned, purified, and characterized recombinant Bet v 1-homologous allergens from green (Actinidia deliciosa, Act d 8) and gold (Actinidia chinensis, Act c 8) kiwifruit, and confirmed the presence of its natural counterpart by inhibition assays. Well-characterized recombinant Act d 8 and Act c 8 were recognized by birch pollen/kiwifruit (confirmed by double-blind placebo-controlled food challenge) allergic patients in IgE immunoblots and ELISA experiments. The present data point out that Bet v 1 homologues are allergens in kiwifruit and of relevance for patients sensitized to tree pollen and kiwifruit, and might have been neglected so far due to low abundance in the conventional extracts used for diagnosis. [source] Manganese-enhanced magnetic resonance imaging (MEMRI)NMR IN BIOMEDICINE, Issue 8 2004Alan P. Koretsky Abstract Manganese ion (Mn2+) is an essential metal that participates as a cofactor in a number of critical biological functions, such as electron transport, detoxification of free radicals and synthesis of neurotransmitters. Mn2+ can enter excitable cells using some of the same transport systems as Ca2+ and it can bind to a number of intracellular sites because it has high affinity for Ca2+ and Mg2+ binding sites on proteins and nucleic acids. Paramagnetic forms of manganese ions are potent MRI relaxation agents. Indeed, Mn2+ was the first contrast agent proposed for use in MRI. Recently, there has been renewed interest in combining the strong MRI relaxation effects of Mn2+ with its unique biology, in order to further expand the already broad assortment of useful information that can be measured by MRI. Such an approach has been continuously developed in the past several years to provide unique tissue contrast, to assess tissue viability, to act as a surrogate marker of calcium influx into cells and to trace neuronal connections. This special issue of NMR in Biomedicine on manganese-enhanced MRI (MEMRI) is aimed at providing the readers of this journal with an extensive review of some of the most prominent applications of MEMRI in biological systems. Written by several of the leaders in the field, the reviews and original research articles featured in this special issue are likely to offer an exciting and inspiring view of the broad range of applications of MEMRI. Copyright © 2004 John Wiley & Sons, Ltd. [source] China's "Soft" Naval Power in the Indian OceanPACIFIC FOCUS, Issue 1 2010Toshi Yoshihara For the past several years, Beijing has been attempting to "shape" the diplomatic and strategic environment in maritime Asia, projecting an image of itself as an innately trustworthy great power. As a part of this public relations campaign, Chinese leaders have retailed the story of Zheng He, the Ming Dynasty eunuch admiral who voyaged to destinations throughout the South China Sea and the Indian Ocean six centuries ago. They have touted the feats of Zheng He, who sojourned in maritime Asia without attempting military conquest, as a metaphor for China's current peaceful ascent in the maritime domain. In doing so, Beijing hopes to convince key audiences in Southeast Asia and South Asia that it remains pacific in outlook , and thus can be counted on not to abuse the sea power it is amassing. An attractive vision of China, they hope, will avert the tendency of regional states to band together to balance Chinese power. Until now, Chinese diplomats have had the luxury of telling their story how they wanted to, as deployments of China's naval forces beyond East Asia remained abstract. Beijing neither saw the need nor boasted the capacity to maintain strong forces far from Chinese shores. However, the headline-grabbing dispatch of two destroyers and a combat logistics ship to the Gulf of Aden on counter-piracy duty in late 2008 has put China squarely in the spotlight. By depicting itself as an inherently defensive power, China has set a high standard for its behavior at sea. Fellow Asian powers will hold Beijing to this lofty benchmark , measuring its actions against the storyline Chinese leaders have developed around Zheng He's voyages. Beijing's anti-piracy mission thus offers an ideal opportunity to empirically test the efficacy of Chinese soft power at sea. To this end, this paper explores the motives behind the Zheng He narrative and assesses the key messages that Chinese leaders are attempting to convey to Asian capitals. This study then examines the extent to which China's unprecedented naval presence in the Indian Ocean has dovetailed with the Zheng He storyline and with the larger strategy of easing regional misgivings about Chinese maritime power. Finally, the paper analyzes how India, a target audience, is responding to China's narrative, drawing several preliminary conclusions about the effectiveness and the prospects of Chinese soft power in the Indian Ocean. [source] Transforming the US,ROK Alliance: Changes in Strategy, Military and Bases,PACIFIC FOCUS, Issue 1 2009Jae-Jung Suh Since the waning days of the Cold War, the US,ROK alliance has gone through a number of changes. Its transformation has accelerated for the past several years in no small part due to the Bush administration's new strategy, military transformation, and global base realignment as well as the Roh government's desire for self-reliant defense. This article outlines the ways in which the three changes have affected the alliance, and assesses the impacts they are likely to have on the security of the Korean peninsula and Northeast Asia. It concludes with a consideration of the modifications that the new governments in Seoul and Washington are likely to make to the transformation of the alliance in the near future. [source] Polychromatic Light Similar to the Terrestrial Solar Spectrum Without its UV Component Stimulates DNA Synthesis in Human Peripheral Blood Lymphocytes In Vivo and In VitroPHOTOCHEMISTRY & PHOTOBIOLOGY, Issue 5 2006Natalya A. Zhevago ABSTRACT Immunosuppressive effects of the minor component of the terrestrial solar spectrum, UV radiation, have been substantiated over the past several years. This raises the question of what influence the dominant part of the solar spectrum,visible and IR light,would have on the human immune system. In the present randomized, placebo-controlled double-blind study a small area of the body surface of volunteers was irradiated with polychromatic light (480,3400 nm), simulating the significant part of the terrestial sunlight irradiance spectrum and its power density. An average 2.5-fold to three-fold increase in spontaneous and phytohemagglutinin-induced DNA synthesis in peripheral blood lymphocytes (Lym) was revealed at 0.5,24 h after irradiation at a therapeutic dose (12 J/cm2) in subjects with low preirradiation levels of both processes. The in vivo findings were echoed in parallel in vitro experiments, when blood drawn from the same subjects was directly irradiated (2.4 J/cm2), or when the irradiated blood was mixed 1:10 with nonirradiated autolo-gous blood to model events in the circulation following transcutaneous blood photomodification. Our data suggest that exposure of the human body to polychromatic visible + IR light may photomodify blood in the dermal vasculature of the irradiated area to lead to an immediate transfer of the light-induced effects to Lym of the entire circulating blood, which can result in modulation of Lym functional state at the systemic level. [source] On Spectral Relaxation in Proteins,,§PHOTOCHEMISTRY & PHOTOBIOLOGY, Issue 4 2000Joseph R. Lakowicz ABSTRACT During the past several years there has been debate about the origins of nonexponential intensity decays of intrinsic tryptophan (trp) fluorescence of proteins, especially for single tryptophan proteins (STP). In this review we summarize the data from diverse sources suggesting that time-dependent spectral relaxation is a ubiquitous feature of protein fluorescence. For most proteins, the observations from numerous laboratories have shown that for trp residues in proteins (1) the mean decay times increase with increasing observation wavelength; (2) decay associated spectra generally show longer decay times for the longer wavelength components; and (3) collisional quenching of proteins usually results in emission spectral shifts to shorter wavelengths. Additional evidence for spectral relaxation comes from the time-resolved emission spectra that usually shows time-dependent shifts to longer wavelengths. These overall observations are consistent with spectral relaxation in proteins occurring on a subnanosecond timescale. These results suggest that spectral relaxation is a significant if not dominant source of nonexponential decay in STP, and should be considered in any interpretation of nonexponential decay of intrinsic protein fluorescence. [source] | ||||||||||||||||||||||||||||