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Parental Clones (parental + clone)
Selected AbstractsHeritability of Phenols in the Resistance of Theobroma cacao against Phytophthora megakarya, the Causal Agent of Black Pod DiseaseJOURNAL OF PHYTOPATHOLOGY, Issue 9 2007P. F. Djocgoue Abstract The black pod disease caused by Phytophthora megakarya is responsible for 80% of the cocoa production loss in Cameroon. To assess the resistance of cocoa plants against this pathogen, necrotic lesions, phenolic content and qualitative alteration of phenolics were conducted in ICS84 and ICS95 clones (two Trinitario introduced from Trinidad) and their hybrids (families F30 and F25) derived from reciprocal cross breeding between these two parental clones after inoculation. The existence of strong hybrid vigour has been shown. Ninety percentage of the hybrid's genotypes manifested a positive heterosis effect for the development of lesion size. This suggests the existence of hybrid vigour with a genetic additive effect. F3086, F2509, F2552 and F2586 hybrids were characterized by localized lesions. Those hybrids genotypes can be considered as elite clones. In relation to analysis of total phenolics and lesion size, no maternal effect was detected in the transmission of these characters. A significant and negative correlation (r = ,0.683) (P < 0.01) has been observed between necrosis evolution and phenolics accumulation. The values of the heritability of lesion size and the total phenolic content in offsprings did not permit to show the maternal effect. Qualitative analyses of phenolics showed high flavonones content in cocoa leaves. Qualitative analyses of phenolics in ICS84, ICS95 clones and their hybrids showed a modification of the phenolics profiles, notably concerning apigenin and luteolin derivatives due to the inoculation. These compounds, along with others that were not identified, could have a role in the reaction and mechanism of defence of cocoa against P. megakarya. [source] Inheritance of useful traits in cassava grown in subhumid conditionsPLANT BREEDING, Issue 2 2006N. T. Cach Abstract A diallel study among nine parental clones of cassava was conducted in the subhumid environment on the northern coast of Colombia. Analysis of variance suggested significant effects for the six variables analysed: fresh-root yield, harvest index, root dry matter content, height of first branching, reaction to thrips and plant-type scores. General and specific combining ability effects and their interaction with the environment were significant for most of the variables as well. Results suggested that dominance plays a particularly important role in the cases of fresh-root yield and harvest index but had relatively little importance in the reaction to thrips, dry matter content or height of first branching. Specific breeding approaches are suggested for these traits, depending on the relative importance of additive or non-additive effects in their inheritance. The correlations among different traits were also analysed and in several cases their magnitude reached statistical and biological significance. [source] Characterization of gene localization and accessibility in DHFR-amplified CHO cellsBIOTECHNOLOGY PROGRESS, Issue 1 2009Zhou Jiang Abstract Efficient transcription is critical for high yields of recombinant proteins by mammalian cells. We previously reported that dihydrofolate reductase (DHFR)-mediated gene amplification can augment transcriptional rates as well as increasing gene copy numbers in Chinese hamster ovary (CHO) cells.1 In an attempt to elucidate the mechanisms involved, we have employed several approaches to identify the epigenetic differences between cell clones with varying transcriptional rates. Transgene placement and accessibility varies between unrelated parental cell clones with differential transcriptional rates. However, we did not observe any apparent epigenetic differences between parental clones and their amplified progeny, indicating undiscovered regulatory mechanisms are responsible for the augmentation of transcriptional rates upon DHFR-mediated amplification. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009 [source] Limitations to the Development of Humanized Antibody Producing Chinese Hamster Ovary Cells Using Glutamine Synthetase-Mediated Gene AmplificationBIOTECHNOLOGY PROGRESS, Issue 3 2006Seung Chul Jun Recombinant Chinese hamster ovary (CHO) cells expressing a humanized antibody were obtained by transfection of an antibody expression vector (pKC-GS-HC-huS) into CHO-K1 cells and subsequent glutamine synthetase (GS)-mediated gene amplification in media containing different concentrations of methionine sulfoximine (MSX). Concentrations consisted of 25, 200, 500, and 1000 ,M of MSX. The highest producer (HP) subclones were isolated from each MSX level by the limiting dilution method and were characterized with respect to antibody production. No positive relationship was observed between specific antibody productivity (qAb) and MSX concentration. Furthermore, it was found that the antibody production stability of these subclones was very poor even in the presence of selection pressure. During long-term cultures in the presence of the corresponding concentrations of MSX, qAb of all HP subclones significantly decreased for the first six passages and thereafter stabilized. Southern and slot blot analyses showed that the loss of antibody gene copies was only partially responsible for the decreased qAb. Fluorescence in situ hybridization (FISH) analysis revealed some cytogenetic features indicative of antibody production instability. Unstable chromosomal structures including dicentrics, rings, and extremely long chromosomes were observed. Amplified sequences enclosed in nuclear projections were often observed. The telomeric repeat sequence, which may be involved in the stabilization of amplified arrays, was found to be absent at the ends of most marker chromosomes. Furthermore, FISH analysis revealed that the overall chromosome content was duplicated in some HP subclones. When metaphase of 12 high producing parental clones was examined, the frequency of occurrence of the polyploidy was 25%. Taken together, the data obtained here suggests that instability could be a concern in the development of CHO cells with GS-mediated gene amplification. [source] | ||||||||||