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P Availability (p + availability)
Selected AbstractsPhosphorus-limited growth dynamics in two Baltic Sea cyanobacteria, Nodularia sp. and Aphanizomenon sp.FEMS MICROBIOLOGY ECOLOGY, Issue 3 2006Jenny Degerholm Abstract Rates of carbon (C) specific growth and nitrogen (N2) fixation were monitored in cultures of Baltic Sea Nodularia and Aphanizomenon exposed to gradual limitation by inorganic phosphorus (P). Both cyanobacteria responded by decreased cellular P content followed by lowered rates of growth and N2 fixation. C-specific growth and cellular N content changed faster in Aphanizomenon both when inorganic P was lowered as well as during reintroduction of P. Aphanizomenon also showed a more rapid increase in N-specific N2 fixation associated with increased C-specific growth. When ambient concentrations of inorganic P declined, both cyanobacteria displayed higher rates of alkaline phosphatase (APase) activity. Lower substrate half-saturation constants (KM) and higher Vmax : KM ratio of the APase enzyme associated with Nodularia suggest a higher affinity for dissolved organic P (DOP) substrate than Aphanizomenon. Aphanizomenon, which appears more sensitive to changes in ambient dissolved inorganic P, may be adapted to environments with elevated concentrations of P or repeated intrusions of nutrient-rich water. Nodularia on the other hand, with its higher tolerance to increased P starvation may have an ecological advantage in stratified surface waters of the Baltic Sea during periods of low P availability. [source] Could rising aquatic carbon dioxide concentrations favour the invasion of elodeids in isoetid-dominated softwater lakes?FRESHWATER BIOLOGY, Issue 9 2009P. SPIERENBURG Summary 1. During the past century, isoetid vegetation types in softwater lakes have often been invaded by faster-growing elodeids. In these C-limited systems, this may be related to rising aquatic CO2 levels. 2. In a laboratory experiment we tested the growth response of two elodeid species, Myriophyllum alterniflorum and Callitriche hamulata, at four different CO2 levels, ranging from 20 to 230 ,mol L,1. In addition, we tested the effect of the nutrient status of the sediment on the growth of C. hamulata at the different CO2 levels. 3. Shoot and root growth increased with rising CO2 availability. Irrespective of sediment type, growth was minimal to negative at the lowest CO2 treatment level, while becoming positive at CO2 levels around 40,50 ,mol L,1. Substantial growth was only obtained when the macrophytes were growing on mesotrophic sediments. The plants reached close to maximal growth at CO2 levels of c. 100 ,mol L,1. 4. Within this experiment, the growth of C. hamulata at CO2 levels above 90 ,mol L,1 may have been limited by N and P availability in both sediment types. The growth rate of M. alterniflorum did not seem to be limited by N and P availability, most likely due to its much higher relative root production. 5. The experimental results show that neither M. alterniflorum nor C. hamulata is able to invade isoetid-dominated softwater lakes at very low aquatic CO2 concentrations. However, if the sediments contain enough nutrients, a rise in aquatic CO2 could allow the invasion of elodeid species leading to the subsequent disappearance of slow-growing isoetids. [source] The effects of Daphnia on nutrient stoichiometry and filamentous cyanobacteria: a mesocosm experiment in a eutrophic lakeFRESHWATER BIOLOGY, Issue 7 2002M. J. PATERSON 1.,Stoichiometric theory predicts that the nitrogen : phosphorus (N : P) ratio of recycled nutrients should increase when P-rich zooplankton such as Daphnia become dominant. We used an enclosure study to test the hypothesis that an increased biomass of Daphnia will increase the relative availability of N versus P sufficiently to decrease the abundance of filamentous cyanobacteria. The experiment was conducted in artificially enriched Lake 227 (L227) in the Experimental Lakes Area (ELA), north-western Ontario, Canada. Previous studies in L227 have shown that the dominance of filamentous, N-fixing cyanobacteria is strongly affected by changes in the relative loading rates of N and P. 2.,We used a 2 × 2 factorial design with the addition or absence of D. pulicaria and high or low relative loading rates of N and P (+NH4, ,NH4) in small enclosures as treatment variables. If Daphnia can strongly affect filamentous cyanobacteria by altering N and P availability, these impacts should be greatest with low external N : P loading rates. The phytoplankton community of L227 was predominantly composed of filamentous Aphanizomenon spp. at the start of the experiment. 3.,Daphnia strongly reduced filamentous cyanobacterial density in all enclosures to which they were added. The addition of NH4 had only a small impact on algal community composition. Hence, we conclude that Daphnia did not cause reductions in cyanobacteria by altering the N : P ratio of available nutrients. 4.,Despite the lack of evidence that Daphnia affected filamentous cyanobacteria by altering the relative availability of N and P, we found changes in nutrient cycling consistent with other aspects of stoichiometric theory. In the presence of Daphnia, total P in the water column decreased because of an increase in P sedimentation. In contrast to P, a decrease in suspended particulate N was offset by an increase in dissolved N (especially NH4). Hence, dissolved and total N : P ratios in the water column increased with Daphnia as a result of differences in the fate of suspended particulate N versus P. There was minimal accumulation and storage of P in Daphnia biomass in the enclosures. 5.,Our experiment demonstrated that Daphnia can strongly limit filamentous cyanobacterial abundance and affect the biogeochemical cycling of nutrients. In our study, changes in nutrient cycling were apparently insufficient to cause the changes in phytoplankton community composition that we observed. Daphnia therefore limited filamentous cyanobacteria by other mechanisms. [source] Seasonal changes in herbage production and soil phosphorus contents in Japanese lawngrass (Zoysia japonica Steud.) and tall fescue (Festuca arundinacea Schreb.) pasturesGRASSLAND SCIENCE, Issue 1 2008Makoto Kaneko Abstract Seasonal changes in the above-ground phosphorus (P), soil total P (TP), soil Olsen P (OP) and soil microbial biomass P (MBP) were investigated for 2 years in Japanese lawngrass (Zy) and tall fescue (Tf) pastures on Japanese Andosol, with the goal of clarifying P characteristics in the Zy pasture in comparison with the Tf pasture. The soil P attributes were measured in two soil layers (root mat layer, 0,2.5 cm depth; under layer, 5,10 cm depth). The P concentration of the above-ground herbage in the Zy pasture, which was higher than the standard value and similar to those in the Tf pasture, might have contributed to the large amounts of the above-ground P mass. The lack of plowing management and the coverage with Japanese lawngrass might have changed soil TP. The TP, the OP and the OP/TP in the Zy pasture were higher than those in the Tf pasture, and the TP, the OP and the OP/TP at the root mat layer were higher than those at the under layer. A large amount of the TP and high P availability in the soil caused the large amounts of OP. Soil pH, soil microorganisms and MBP might have affected soil P availability in the Zy pasture. Plant litter in the root mat layer of the Zy pasture may have increased soil P accumulation and its availability, which might be reasons for the high P uptake in the present study. Japanese lawngrass pasture may be a system with improved soil P utilization efficiency based on P cycling. [source] Influence of Tithonia diversifolia and triple superphosphate on dissolution and effectiveness of phosphate rock in acidic soilJOURNAL OF PLANT NUTRITION AND SOIL SCIENCE, Issue 5 2006Isaac Savini Abstract An incubation and a pot experiment were conducted to evaluate the dissolution and agronomic effectiveness of a less reactive phosphate rock, Busumbu soft ore (BPR), in an Oxisol in Kenya. Resin (anion and anion + cation)-extractable P and sequentially extracted P with 0.5 M NaHCO3, 0.1 M NaOH, and 1 M HCl were analyzed. Dissolution was determined from the increase in anion resin (AER),, NaHCO3 -, and NaOH-extractable P in soil amended with PR compared with the control soil. Where P was applied, resin P significantly increased above the no-P treatment. Busumbu-PR solubility was low and did not increase significantly in 16 weeks. Anion + cation (ACER)-extractable P was generally greater than AER-P. The difference was greater for PR than for triple superphosphate (TSP). The ACER extraction may be a better estimate of plant P availability, particularly when poorly soluble P sources are used. Addition of P fertilizers alone or in combination with Tithonia diversifolia (TSP, BPR, TSP + Tithonia, and BPR + Tithonia) increased the concentration of labile inorganic P pools (NaHCO3 - and NaOH-Pi). Cumulative evolved CO2 was significantly correlated with cumulative N mineralized from Tithonia (r, 0.51, p < 0.05). Decrease in pH caused NH -N accumulation while NO -N remained low where Tithonia was incorporated at all sampling times. However, when pH was increased, NH -N declined with a corresponding rise in NO -N. Tithonia significantly depressed soil exchangeable acidity relative to control with time. A significant increase (p < 0.05) was observed for P uptake but not dry-mass production in maize where BPR was applied. The variations in yield and P uptake due to source and rates of application were statistically significant. At any given P rate, highest yields were obtained with Tithonia alone. Combination of Busumbu PR with TSP or Tithonia did not enhance the effectiveness of the PR. The poor dissolution and plant P uptake of BPR may be related to the high Fe content in the PR material. [source] Effect of phosphate fertilization on crop yield and soil phosphorus status,JOURNAL OF PLANT NUTRITION AND SOIL SCIENCE, Issue 5 2003Anne Gallet Abstract To evaluate the effect of three phosphorus (P) fertilization regimes (no P, P input equivalent to P off-take by crops, P input higher than P off-take) on crop yield, P uptake, and soil P availability, seven field experiments (six in crop rotations, one under permanent grassland) were conducted in Switzerland during nine years (six trials) or 27 years (one trial). Soil total P (Pt), inorganic P (Pi), organic P (Po), and the amount of isotopically exchangeable soil P were measured in the 0,20,cm and 30,50,cm layers of the arable soils and in the 0,10,cm layer of the permanent grassland soil. Omitting P fertilization resulted in significant yield decreases only in one field crop trial as the amount of P isotopically exchangeable within one minute (E1min) reached values lower than 5 mg P (kg soil),1. In the absence of P fertilization Pi decreased on average from 470 to 410 mg P (kg soil),1 in the upper horizon of 6 sites while Po decreased only at two sites (from 510 to 466 mg P (kg soil),1 on average). In all the treatments of the trials started in 1989 the E1min values of the upper horizon decreased on average from 15.6 to 7.4 mg P (kg soil),1 between 1989 and 1998. These decreases were also observed when P inputs were higher than crops needs, showing that in these soils the highest P inputs were not sufficient to maintain the high initial available P levels. Finally for the six arable trials the values of the isotopic exchange kinetics parameters (R/r1, n, CP) and P exchangeable within 1 minute (E1min) at the end of the experiment could be estimated from the values measured at the beginning of trial and the cumulated P balance. Der Einfluss der Phosphordüngung auf den Pflanzenertrag und den Phosphorstatus des Bodens Die Begrenzung der Düngung mit Phosphat (P) bei Böden, die bereits hohe Gehalte an verfügbarem P aufweisen, kann zur Verringerung der P-Verluste in Oberflächen- und Grundwasser beitragen. Sieben Feldversuche (6 Versuche mit Ackerkulturen, 1 Versuch mit Dauerwiese) wurden während 9 Jahren (6 Versuche) resp. 27 Jahren (1 Versuch) durchgeführt mit dem Ziel, den Einfluss von drei Düngungsraten (keine P-Gabe, P-Gabe entsprechend dem P-Entzug durch die Pflanzen und P-Gabe höher als der P-Entzug durch die Pflanzen) auf Pflanzenertrag, P-Aufnahme und P-Verfügbarkeit zu studieren. Die Gehalte an anorganischem P (Pi) und organischem P (Po), sowie deren Summe (Pt) und die Gehalte an isotopisch austauschbarem P wurden in den Bodentiefen von 0,20,cm und 30,50,cm der ackerbaulichen Versuche und in einer Bodentiefe von 0,10,cm in der Dauerwiese gemessen. Der Verzicht auf P-Düngung führte nur in einem der ackerbaulichen Versuche zu einer signifikanten Abnahme des Pflanzenertrags, wobei der Gehalt an isotopisch austauschbarem P (E1min) unter 5 mg P (kg Boden),1 fiel. Pi sank bei Verzicht auf P-Düngung im oberen Horizont von 6 Böden durchschnittlich von 470 auf 410 mg P (kg Boden),1, während Po nur in 2 Böden absank (durchschnittlich von 510 auf 466 mg P (kg Boden),1). Ueber alle Düngungsverfahren jener Versuche, die 1989 begonnen hatten, sank E1min im oberen Horizont von 1989 bis 1998 durchschnittlich von 15.6 auf 7.4 mg P (kg Boden),1. Die Abnahme wurde auch beobachtet, wenn die P-Gabe höher war als der P-Entzug durch die Pflanzen, was zeigt, dass in diesen Böden selbst die höchste P-Gabe nicht genügte, um den ursprünglich hohen Gehalt an verfügbarem P aufrecht zu erhalten. Schließlich wurden in den 6 ackerbaulichen Versuchen die Parameter der Isotopenaustauschkinetik (R/r1, n, CP) und E1min am Ende des Versuches mit den ursprünglichen Werten zu Beginn des Versuchs verglichen. Es ergaben sich signifikante Beziehungen zwischen den Werten am Ende des Versuchs einerseits und den ursprünglichen Werten und der P-Bilanz andererseits. [source] Phosphorus status on Canadian organic farmsJOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 15 2007Ralph C Martin Abstract In eastern North America, many conventional livestock farms, especially dairy farms with high inputs of feed and fertiliser have excess soil phosphorus levels and an annual phosphorus surplus. However, a Canadian dairy farm in transition to organic, without fertiliser inputs reduced its farm P surplus to a marginal level. On long-term organic dairy farms in Ontario, most soils tested low to very low in available P as measured by a standard soil test. Canadian Prairie organic grain farms also consistently demonstrate deficiencies in available soil P. Organic producers have few viable alternatives for P management. Phosphate rock can be acceptable to organic standards (provided they are low in heavy metals, and not processed synthetically), but the P in these becomes available slowly, especially in high pH soils common on most organic farms in Canada. An alternative is to increase soil P availability. Enhanced microbial activity in organically managed soils may make P more available. Livestock manures are rich sources of available phosphorus, but a majority of organic farmers in Canada do not keep livestock. Off-farm manure sources are subject to organic regulations and hauling costs, both of which may be prohibitive. Furthermore, manure from conventional farms in Canada may be contaminated by genetically modified material from corn and soybean feed. Additional research is required to improve short-term availability of soil P and long-term replacement. Copyright © 2007 Society of Chemical Industry [source] Nitrogen and phosphorus availability limit N2 fixation in beanNEW PHYTOLOGIST, Issue 2 2000E. O. LEIDI Availability of nitrogen (N) and phosphorus (P) might significantly affect N2 fixation in legumes. The interaction of N and P was studied in common bean (Phaseolus vulgaris), considering their effects on nodulation and N2 fixation, nitrate reductase activity, and the composition of N compounds in xylem sap. The effect of N on the uptake of P by plants was estimated by analysing rhizospheric pH and P concentration in xylem sap and in plant shoots. Inoculated bean plants were grown in pots containing perlite/vermiculite in two experiments with different amounts of P and N. In a third experiment, bean plants were grown on two soil types or on river sand supplied with different concentrations of N. At harvest, shoot growth, number of nodules and mass, and nitrogenase activity were determined. Xylem sap was collected for the determination of ureides, amino acids, nitrate and phosphate concentration. At low nitrate concentration (1 mM), increasing amounts of P promoted both nodule formation and N2 fixation, measured as ureide content in the xylem sap. However, at high nitrate concentration (10 mM), nodulation and N2 fixation did not improve with increased P supply. Glutamine and aspartate were the main organic N compounds transported in the xylem sap of plants grown in low nitrate, whereas asparagine was the dominant N compound in xylem sap from plants grown in high nitrate. Nitrate reductase activity in roots was higher than in shoots of plants grown with low P and high N. In both soils and in the sand experiment, increased application of N decreased nodule mass and number, nitrogenase activity and xylem ureides but increased the concentration of asparagine in xylem sap. Increasing P nutrition improved symbiotic N2 fixation in bean only at low N concentrations. It did not alleviate the inhibitory effect of high nitrate concentration on N2 fixation. A decrease in plant P uptake was observed, as indicated by a lower concentration of P in the xylem sap and shoots, correlating with the amount of N supplied. Simultaneously with the specific inhibition of N2 fixation, high nitrate concentrations might decrease P availability, thus inhibiting even further the symbiotic association because of the high P requirement for nodulation and N2 fixation. [source] Litter Decomposition Within the Canopy and Forest Floor of Three Tree Species in a Tropical Lowland Rain forest, Costa RicaBIOTROPICA, Issue 3 2010Catherine L. Cardelús ABSTRACT The rain forest canopy hosts a large percentage of the world's plant biodiversity, which is maintained, in large part, by internal nutrient cycling. This is the first study to examine the effects of site (canopy, forest floor) and tree species (Dipteryx panamensis, Lecythis ampla, Hyeronima alchorneoides) on decay rates of a common substrate and in situ leaf litter in a tropical forest in Costa Rica. Decay rates were slower for both substrates within the canopy than on the forest floor. The slower rate of mass loss of the common substrate in the canopy was due to differences in microclimate between sites. Canopy litter decay rates were negatively correlated with litter lignin:P ratios, while forest floor decay rates were negatively correlated with lignin concentrations, indicating that the control of litter decay rates in the canopy is P availability while that of the forest floor is carbon quality. The slower cycling rates within the canopy are consistent with lower foliar nutrient concentrations of epiphytes compared with forest floor-rooted plants. Litter decay rates, but not common substrate decay rates, varied among tree species. The lack of variation in common substrate decay among tree species eliminated microclimatic variation as a possible cause for differences in litter decay and points to variation in litter quality, nutrient availability and decomposer community of tree species as the causal factors. The host tree contribution to canopy nutrient cycling via litter quality and inputs may influence the quality and quantity of canopy soil resources. Abstract in Spanish is available at http://www.blackwell-synergy.com/loi/btp [source] | |||||||||||||