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Overlapping Bands (overlapping + bands)
Selected AbstractsQualification and Quantification of Fish Protein in Prepared Surimi CrabstickJOURNAL OF FOOD SCIENCE, Issue 5 2008Z.H. Reed ABSTRACT:, Species identification and protein quantification in surimi crabstick were achieved using sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). When the Lowry and Kjeldahl protein determination methods were compared, the former showed more consistent results. Densitometric scanning of the gels was used for quantification of total fish protein as well as total egg white protein. The lower molecular weight proteins, 30 kDa and lower, proved to be the most useful in fish species identification as well as egg white protein addition. Using a combination of the myosin heavy chain band and the species-specific myosin light chain (Alaska pollock: 22.5 kDa; Pacific whiting: 24.4 kDa) proved the most accurate in calculating fish protein content of the crabstick sample, while for those samples that contained egg white, quantification was accomplished from the densitometric analysis of the overlapping bands of actin (45 kDa) from fish and ovalbumin from egg white. Lysozyme (14.3 kDa) proved to be a unique protein band in determining the presence of egg white when the content of dried egg white was equal to or exceeded 0.5% of the total weight of the final crabstick. [source] Raman spectroscopic study of the vivianite arsenate mineralsJOURNAL OF RAMAN SPECTROSCOPY, Issue 10 2003Ray L. Frost Abstract The molecular structures of the vivianite-type arsenate minerals were studied using a combination of Raman and infrared spectroscopy. The Raman spectra of the hydroxyl-stretching regions are complex with overlapping bands at 3419, 3209, 3185 and 3010 cm,1. This complexity is reflected in the water HOH bending modes with strong infrared bands in the 1660,1685 cm,1 region indicating strong hydrogen bonding to arsenate anions in adjacent layers. The Raman arsenate AsO stretching region shows strong similarity between the vivianite arsenate minerals. In the infrared spectra complexity exists with multiple antisymmetric stretching vibrations observed, indicating a reduction of symmetry. Strong infrared bands around 700 and 560 cm,1 are attributed to librational modes of water. Vibrational spectra enable the structure of the minerals to be determined and, whilst similarities exist in the spectral patterns, sufficient differences exist to determine the identification of the minerals. In particular, Raman spectroscopy assists in the identification of the complex isomorphous substitution in these vivianite arsenate minerals. Copyright © 2003 John Wiley & Sons, Ltd. [source] Pressure- and temperature-dependent Raman studies of KNbW2O9 hexagonal tungsten bronzeJOURNAL OF RAMAN SPECTROSCOPY, Issue 3 2003czka Abstract Pressure- and temperature-dependent Raman studies were performed on KNbW2O9 ferroelectric hexagonal tungsten bronze single crystal. The results suggest that some structural changes, possibly phase transitions, connected with tilting of the WO6 octahedra occur in this material at around 290,350 K and 1.0,1.4 GPa. No changes in the spectra could be observed at the 523 and 543,553 K phase transitions. The data indicate that these phase transitions lead to only weak changes in the vibrational properties of KNbW2O9, which were not observed owing to the large bandwidth of the Raman bands. The Raman studies suggest, therefore, that the structure of the unknown phase, existing below 553 K, differs slightly form the orthorhombic structure present above 553 K. The pressure-dependent study revealed that the 52 cm,1 band, assigned to external vibrations of the WO6 octahedron, consists at ambient temperature and pressure of two overlapping bands. These bands become well separated at high pressure. Copyright © 2003 John Wiley & Sons, Ltd. [source] Combined Wavelet Transform with Curve-fitting for Objective Optimization of the Parameters in Fourier Self-deconvolutionCHINESE JOURNAL OF CHEMISTRY, Issue 10 2001Xiu-Qi Zhang Abstract Fourier self-deconvolution was the most effective technique in resolving overlapping bands, in which deconvolution function results in deconvolution and apodization smoothes the magnified noise. Yet, the choice of the original half-width of each component and breaking point for truncation is often very subjective. In this paper, the method of combined wavelet transform with curve fitting was described with the advantages of an enhancement of signal to noise ratio as well as the improved fitting condition, and was applied to objective optimization of the original half-widths of components in unresolved bands for Fourier self-deconvolution. Again, a noise was separated from a noisy signal by wavelet transform, therefore, the breaking point of apodization function can be determined directly in frequency domain. Accordingly, some artifacts in Fourier self-deconvolution were minimized significantly. [source] | ||||||||||