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Ovarian Proteins (ovarian + protein)
Selected AbstractsAltered actin polymerization of Plutella xylostella (L.) in response to ovarian calyx components of an endoparasitoid Cotesia plutellae (Kurdjumov)PHYSIOLOGICAL ENTOMOLOGY, Issue 2 2009MADANAGOPAL NALINI AbstractCotesia plutellae (Kurdjumov) (Hymenoptera: Braconidae), a solitary braconid endoparasitoid wasp, parasitizes the diamondback moth Plutella xylostella (L.) (Lepidoptera: Yponomeutidae) by suppressing the host defense response, thereby resulting in successful parasitization. During parasitization, ovarian calyx fluid is also delivered into the haemocoel of the host along with the wasp egg. The effect of calyx fluid constituents on haemocyte-spreading behaviour of P. xylostella is analysed by measuring F-actin development in the haemocytes. For this purpose, the calyx fluid of C. plutellae is separated into ovarian protein and C. plutellae bracovirus (CpBV). The ovarian protein consists of a wide range of molecular weight proteins, which are apparently different from those of CpBV. When nonparasitized P. xylostella haemocytes are incubated with either ovarian protein or CpBV for 1 or 2 h, haemocytes lose their responsiveness to a cytokine, plasmatocyte-spreading peptide, in a dose-dependent manner for each calyx component and fail to exhibit haemocyte-spreading behaviour. Some CpBV genes are expressed within 1 h of parasitization. The inhibition of haemocyte-spreading could be explained by measuring F-actin contents, in which parasitization by C. plutellae inhibits F-actin development in the haemocytes of P. xylostella. Either ovarian protein or CpBV could inhibit F-actin development in the nonparasitized haemocytes. In addition, co-incubation of ovarian protein and CpBV results in significant additive inhibition of both haemocyte-spreading and F-actin development in the haemocytes in response to cytokine. These results suggest that both components of C. plutellae calyx fluid function in a synergistic manner, leading to immunosuppression during the early stage of parasitization. [source] Role of venom and ovarian proteins in immune suppression of Ostrinia furnacalis (Lepidoptera: Pyralidae) larvae parasitized by Macrocentrus cingulum (Hymenoptera: Braconidae), a polyembryonic parasitoidINSECT SCIENCE, Issue 2 2007YONG LI Abstract Venom and ovarian proteins in braconid and ichneumonid wasps play an important role in the successful parasitism of their host, especially for immune suppression immediately after oviposition. In this study, we compared the effect of venom and ovarian proteins collected from the female wasps of Macrocentrus cingulum, a polyembryonic parasitoid of the larvae of Ostrinia funacalis, on the encapsulation capacity of Sephadex A-25 beads at 4 h and 24 h post-injection both in vivo and in vitro. The results showed that the ovarian proteins significantly interfered with the encapsulation capacity of hemocytes in a dose-dependent manner. Spreading and viability of hemocytes in O. furnacalis was not disrupted by venom and ovarian proteins at various concentrations injected. It seems likely that the ovarian proteins from M. cingulum play a role in suppressing the encapsulation capacity of host hemocytes. [source] Heat Shock Protein 70 and Sex Steroid Receptors in the Follicular Structures of Induced Ovarian CystsREPRODUCTION IN DOMESTIC ANIMALS, Issue 5 2009NR Salvetti Contents The purpose of this study was to estimate the expression and relative amounts of estrogen (ER) and progesterone receptors (PR) and their isoforms as well as heat shock protein 70 (HSP70) in ovaries of rats with induced cystic ovarian disease (COD). Primary, secondary, tertiary, atretic and cystic follicles were evaluated by immunohistochemistry and total ovarian proteins were analyzed by Western blot. In the granulosa layer, growing and cystic follicles in the treated group have a higher expression of ER, than growing follicles of control individuals. In the theca interna layer, tertiary follicles presented a significantly higher expression of ER, in the treated group. An increase in total ER, protein was detected in the treated group. Granulosa cells of all growing, atretic and cystic follicles show a lower expression of ER, in animals with COD, and the total protein expression of ER, was lower in this group. The expression of PR was lower in the granulosa cell layer of tertiary and cystic follicles in treated animals, and theca interna layer had less intense immunostaining in this group. Although there were no differences in the expression of PR-B by Western blotting, the expression of PR-A was higher and the expression of PR-C was smaller in the treated group. An intense HSP70 immunostaining was observed in the cells of cystic follicles. By Western blotting, higher protein expression of HSP70 was detected in the ovarian samples of the control group than those of the treated ones. Ovaries of animals with COD exhibited an altered steroid receptor expression and subtype balance as compared with control animals, and an increase in HSP70 immunoexpression. [source] | ||||||||||