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Other Nucleotides (other + nucleotide)

Distribution by Scientific Domains
Chemistry50%

Selected Abstracts

DHPLC is superior to SSCP in screening p53 mutations in esophageal cancer tissues

INTERNATIONAL JOURNAL OF CANCER, Issue 1 2005
Osamu Yamanoshita
Abstract Mutations of the p53 tumor-suppressor gene universally occur on exons 5,8 in human cancer. We analyzed these mutations in esophageal cancer tissue from 207 patients in China using 2 methods, single-strand conformation polymorphism (SSCP), one of the most frequently used methods, and the recently developed denaturing high-performance liquid chromatography (DHPLC), and compared their sensitivity and efficiency. Exons 5,8 of p53 were amplified from esophageal cancer tissue genomes, screened for fragments of mutations and polymorphisms by SSCP and DHPLC in a blind study and confirmed by direct sequencing to detect the mutations and polymorphisms. The numbers detected by DHPLC were greater than those detected by SSCP, though the rate of mutations and polymorphisms was lower in SSCP than in DHPLC, which appeared to detect smaller mutations (substitutions and 1 bp insertions/deletions). Of the mutations with substitutions detected by DHPLC but not by SSCP, 50% substituted adenosine for other nucleotides, suggesting that these mutations are often missed when SSCP is used. According to these data, the sensitivity of SSCP and DHPLC was 81% and 97%, respectively, and the specificity was 97% and 85%, respectively. Our results suggest that DHPLC may be recommended over SSCP when screening gene mutations. Thus, rates of p53 mutations and polymorphisms in esophageal cancer tissue in Chinese patients were 49% and 41% by DHPLC and SSCP, respectively. © 2004 Wiley-Liss, Inc. [source]

Molecular imprinting of AMP by an ionic-noncovalent dual approach

JOURNAL OF SEPARATION SCIENCE, JSS, Issue 19 2009
Florent Breton
Abstract In order to mimic recognition properties of adenylate kinase, molecularly imprinted polymers (MIPs) were prepared for adenosine 5,-monophosphate (AMP), a substrate of the enzyme. Different functional monomers interacting with the phosphate moiety were tested, and the MIP giving the best specific binding of AMP was composed with one equivalent of 2-(dimethylamino)ethyl methacrylate and ten equivalents of acrylamide compared to AMP. Packed into solid phase cartridge, this polymer showed similar characteristics than the enzyme, since it was specific for AMP toward other nucleotides. [source]

Dietary nucleotide supplementation enhances growth and immune responses of grouper, Epinephelus malabaricus

AQUACULTURE NUTRITION, Issue 2 2009
Y.-H. LIN
Abstract Basal diet containing 0.5, 1.0, 1.5 and 2.0 g kg,1 mixture of inosine monophosphate (IMP), adenosine monophosphate (AMP), guanosine monophosphate (GMP), uridine monophosphate (UMP) and cytidine monophosphate (CMP) (1 : 1 : 1 : 1 : 1) (mixed-NT; Experiment 1) and 1.5 g kg,1 from each nucleotides and mixed-nucleotides (NT; Experiment 2) were fed to triplicate groups of grouper for 8 weeks. Basal diet without NT was used as control in both Experiments. In Experiment 1, fish fed the diet with 1.5 g mixed-NT kg,1 had higher (P < 0.05) weight gain (WG) than the control group. The superoxide anion (O2,) production ratio was higher in fish fed diets with 1.0,1.5 g mixed-NT kg,1 than the fish fed diets with ,0.5 g mixed-NT kg,1. In Experiment 2, fish fed diets with nucleotides had higher WG than the control group. The O2, production ratio was higher in fish fed the diet with 1.5 g AMP kg,1, followed by fish fed diets with 1.5 g UMP and mixed-NT kg,1, and lowest in the control group. These results suggest that growth and immune responses were enhanced in grouper fed diet with 1.5 g mixed-NT kg,1 diet. Diet with 1.5 g kg,1 of AMP seems to be more beneficial on the immune responses in fish than other nucleotides. [source]

Near-atomic resolution crystal structure of an A-­DNA decamer d(CCCGATCGGG): cobalt hexammine interaction with A-DNA

ACTA CRYSTALLOGRAPHICA SECTION D, Issue 1 2003
Boopathy Ramakrishnan
The structure of the DNA decamer d(CCCGATCGGG) has been determined at 1.25,Å resolution. The decamer crystallized in the tetragonal space group P43212, with unit-cell parameters a = b = 44.3, c = 24.8,Å and one strand in the asymmetric unit. The structure was solved by the molecular-replacement method and refined to Rwork and Rfree values of 16.3 and 18.5%, respectively, for 5969 reflections. The decamer forms the A-form DNA duplex, with the abutting crystal packing typical of A-DNA. The crystal packing interactions seem to distort the local conformation: A5 adopts the trans/trans conformation for the torsion angles , and , instead of the usual gauche,/gauche+ conformations, yielding G*(G·C) base triplets. The highly hydrated [Co(NH3)6]3+ ion adopts a novel binding mode to the DNA duplex, binding directly to phosphate groups and connecting to N7 and O6 atoms of guanines by water bridges. Analysis of thermal parameters (B factors) shows that the nucleotides involved in abutting crystal packing are thermally more stable than other nucleotides in the duplex. [source]