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Oxygen Consumption Rate (oxygen + consumption_rate)
Selected AbstractsDifferences in temperature, organic carbon and oxygen consumption among lowland streamsFRESHWATER BIOLOGY, Issue 12 2005KAJ SAND-JENSEN Summary 1. Temperature, organic carbon and oxygen consumption were measured over a year at 13 sites in four lowlands streams within the same region in North Zealand, Denmark with the objectives of determining: (i) spatial and seasonal differences between open streams, forest streams and streams with or without lakes, (ii) factors influencing the temperature dependence of oxygen consumption rate, (iii) consequences of higher temperature and organic content in lake outlets on oxygen consumption rate, and (iv) possible consequences of forecasted global warming on degradation of organic matter. 2. High concentrations of easily degradable dissolved (DOC) and particulate organic carbon (POC) were found in open streams downstream of plankton-rich lakes, while high concentrations of recalcitrant DOC were found in a forest brook draining a forest swamp. Concentrations of predominantly recalcitrant POC and DOC were low in a groundwater-fed forest spring. Overall, DOC concentration was two to 18 times higher than POC concentrations. 3. Oxygen consumption rate at 20 °C was higher during summer than winter, higher in open than shaded streams and higher in lake outlets than inlets. Rate was closely related to concentrations of chlorophyll and POC but not to DOC. The ratio of oxygen consumption rate to total organic concentrations (DOC + POC), serving as a measure of organic degradability, was highest downstream of lakes, intermediate in open streams and lowest in forest streams. 4. Temperature coefficients describing the exponential increase of oxygen consumption rate between 4 and 20 °C averaged 0.121 °C,1 (Q10 of 3.35) in 70 measurements and showed no significant variations between seasons and stream sites or correlations with ambient temperature and organic content. 5. Oxygen consumption rate was enhanced downstream of lakes during summer because of higher temperature and, more significantly, greater concentrations of degradable organic carbon. Oxygen consumption rates were up to seven times higher in the stream with three impoundments than in a neighbouring unshaded stream and 21 times higher than in the groundwater-fed forest spring. 6. A regional climate model has calculated a dramatic 4,5 °C rise in air temperature over Denmark by 2070,2100. If this is realised, unshaded streams are estimated to become 2,3 °C warmer in summer and winter and 5,7 °C warmer in spring and, thereby, increase oxygen consumption rates at ambient temperature by 30,40% and 80,130%, respectively. Faster consumption of organic matter and dissolved oxygen downstream of point sources should increase the likelihood of oxygen stress of the stream biota and lead to the export of less organic matter but more mineralised nutrients to the coastal waters. [source] Physiological responses of pink abalone Haliotis corrugata (Gray, 1828) exposed to different combinations of temperature and salinityAQUACULTURE RESEARCH, Issue 7 2010Zarina Medina Romo Abstract Physiological responses of pink abalone Haliotis corrugata were determined under different temperature and salinity conditions. Oxygen consumption rate was not affected by temperature and salinity. Ammonium excretion of pink abalone was inversely related to salinity. The O:N ratio indicated that abalone maintained in lower salinities had an interval of 4.9,7.7, which is indicative of a protein-dominated metabolism, whereas the O:N in 35, was 28.8,35.5 for both temperatures, suggesting that carbohydrates were used as energy substrate. Haemolymph osmolality of abalone exposed to 20 and 24 °C was slightly hyperiso-osmoconformic in salinity ranges of 20,35,. The results of this study suggested that for optimized culture, pink abalone should be cultivated at 24 °C at a salinity of 35,. [source] Effect of intermittent starvation on growth and some antioxidant indexes of Macrobrachium nipponense (De Haan)AQUACULTURE RESEARCH, Issue 5 2009Zhi-Hua Li Abstract The effect of different periods of starvation (0, 2, 4 and 8 days) followed by re-feeding on growth, feed utilization, oxygen consumption and some immune indexes [reactive oxygen intermediates (ROIs), activities of superoxide dismutase (SOD) and catalase (CAT)] was evaluated over an 18-day experimental period in shrimp Macrobrachium nipponense (De Haan) that had an initial body weight of 0.52 g. As a result of compensatory growth, indicated by an increase in specific growth rate (SGR), feeding rate (FR) and feed conversion efficiency (FCE) after re-feeding, final body weight of shrimp starved for 2 days (0.63 g) and 4 days (0.65 g) did not differ (P>0.05) from the control group (0.64 g), with feed withholding for 8 days presenting a significant lower value (0.63 g). Oxygen consumption rate (OCR) decreased during the starvation period in all groups, followed by a gradual increase to a similar (P>0.05) value than found in the control group (0.47 mg kg,1 h,1) at the end of the experiment. Although ROIs and the activity of SOD and CAT fluctuated during starvation in the feed-deprived groups, values at the termination of the experiment were comparable (P>0.05) to those found for the control group. [source] Differences in temperature, organic carbon and oxygen consumption among lowland streamsFRESHWATER BIOLOGY, Issue 12 2005KAJ SAND-JENSEN Summary 1. Temperature, organic carbon and oxygen consumption were measured over a year at 13 sites in four lowlands streams within the same region in North Zealand, Denmark with the objectives of determining: (i) spatial and seasonal differences between open streams, forest streams and streams with or without lakes, (ii) factors influencing the temperature dependence of oxygen consumption rate, (iii) consequences of higher temperature and organic content in lake outlets on oxygen consumption rate, and (iv) possible consequences of forecasted global warming on degradation of organic matter. 2. High concentrations of easily degradable dissolved (DOC) and particulate organic carbon (POC) were found in open streams downstream of plankton-rich lakes, while high concentrations of recalcitrant DOC were found in a forest brook draining a forest swamp. Concentrations of predominantly recalcitrant POC and DOC were low in a groundwater-fed forest spring. Overall, DOC concentration was two to 18 times higher than POC concentrations. 3. Oxygen consumption rate at 20 °C was higher during summer than winter, higher in open than shaded streams and higher in lake outlets than inlets. Rate was closely related to concentrations of chlorophyll and POC but not to DOC. The ratio of oxygen consumption rate to total organic concentrations (DOC + POC), serving as a measure of organic degradability, was highest downstream of lakes, intermediate in open streams and lowest in forest streams. 4. Temperature coefficients describing the exponential increase of oxygen consumption rate between 4 and 20 °C averaged 0.121 °C,1 (Q10 of 3.35) in 70 measurements and showed no significant variations between seasons and stream sites or correlations with ambient temperature and organic content. 5. Oxygen consumption rate was enhanced downstream of lakes during summer because of higher temperature and, more significantly, greater concentrations of degradable organic carbon. Oxygen consumption rates were up to seven times higher in the stream with three impoundments than in a neighbouring unshaded stream and 21 times higher than in the groundwater-fed forest spring. 6. A regional climate model has calculated a dramatic 4,5 °C rise in air temperature over Denmark by 2070,2100. If this is realised, unshaded streams are estimated to become 2,3 °C warmer in summer and winter and 5,7 °C warmer in spring and, thereby, increase oxygen consumption rates at ambient temperature by 30,40% and 80,130%, respectively. Faster consumption of organic matter and dissolved oxygen downstream of point sources should increase the likelihood of oxygen stress of the stream biota and lead to the export of less organic matter but more mineralised nutrients to the coastal waters. [source] Relationships between metabolic rate, muscle electromyograms and swim performance of adult chinook salmonJOURNAL OF FISH BIOLOGY, Issue 4 2003D. R. Geist Oxygen consumption rates of adult spring chinook salmon Oncorhynchus tshawytscha increased with swim speed and, depending on temperature and fish mass, ranged from 609 mg O2 h,1 at 30 cm s,1 (c. 0·5 BL s,1) to 3347 mg O2 h,1 at 170 cm s,1 (c. 2·3 BL s,1). Corrected for fish mass, these values ranged from 122 to 670 mg O2 kg,1 h,1, and were similar to other Oncorhynchus species. At all temperatures (8, 12·5 and 17° C), maximum oxygen consumption values levelled off and slightly declined with increasing swim speed >170 cm s,1, and a third-order polynomial regression model fitted the data best. The upper critical swim speed (Ucrit) of fish tested at two laboratories averaged 155 cm s,1 (2·1 BL s,1), but Ucrit of fish tested at the Pacific Northwest National Laboratory were significantly higher (mean 165 cm s,1) than those from fish tested at the Columbia River Research Laboratory (mean 140 cm s,1). Swim trials using fish that had electromyogram (EMG) transmitters implanted in them suggested that at a swim speed of c. 135 cm s,1, red muscle EMG pulse rates slowed and white muscle EMG pulse rates increased. Although there was significant variation between individual fish, this swim speed was c. 80% of the Ucrit for the fish used in the EMG trials (mean Ucrit 168·2 cm s,1). Bioenergetic modelling of the upstream migration of adult chinook salmon should consider incorporating an anaerobic fraction of the energy budget when swim speeds are ,80% of the Ucrit. [source] Regulation of expression of terminal oxidases in Paracoccus denitrificansFEBS JOURNAL, Issue 8 2001Marijke F. Otten In order to study the induction of terminal oxidases in Paracoccus denitrificans, their promoters were fused to the lacZ reporter gene and analysed in the wild-type strain, in an FnrP-negative mutant, in a cytochrome bc1 -negative mutant, and in six single or double oxidase-negative mutant strains. The strains were grown under aerobic, semi-aerobic, and denitrifying conditions. The oxygen-sensing transcriptional-regulatory protein FnrP negatively regulated the activity of the qox promoter, which controls expression of the ba3 -type quinol oxidase, while it positively regulated the activity of the cco promoter, which controls expression of the cbb3 -type cytochrome c oxidase. The ctaDII and ctaC promoters, which control the expression of the aa3 -type cytochrome c oxidase subunits I and II, respectively, were not regulated by FnrP. The activities of the latter two promoters, however, did decrease with decreasing oxygen concentrations in the growth medium, suggesting that an additional oxygen-sensing mechanism exists that regulates transcription of ctaDII and ctaC. Apparently, the intracellular oxygen concentration (as sensed by FnrP) was not the only signal to which the oxidase promoters responded. At given extracellular oxygen status, both the qox and the cco promoters responded to mutations in terminal oxidase genes, whereas the ctaDII and ctaC promoters did not. The change of electron distribution through the respiratory network, resulting from elimination of one or more oxidase genes, may have changed intracellular signals that affect the activities of the qox and cco promoters. On the other hand, the re-routing of electron distribution in the respiratory mutants hardly affected the oxygen consumption rate as compared to that of the wild-type. This suggests that the mutants adapted their respiratory network in such a way that they were able to consume oxygen at a rate similar to that of the wild-type strain. [source] Differences in temperature, organic carbon and oxygen consumption among lowland streamsFRESHWATER BIOLOGY, Issue 12 2005KAJ SAND-JENSEN Summary 1. Temperature, organic carbon and oxygen consumption were measured over a year at 13 sites in four lowlands streams within the same region in North Zealand, Denmark with the objectives of determining: (i) spatial and seasonal differences between open streams, forest streams and streams with or without lakes, (ii) factors influencing the temperature dependence of oxygen consumption rate, (iii) consequences of higher temperature and organic content in lake outlets on oxygen consumption rate, and (iv) possible consequences of forecasted global warming on degradation of organic matter. 2. High concentrations of easily degradable dissolved (DOC) and particulate organic carbon (POC) were found in open streams downstream of plankton-rich lakes, while high concentrations of recalcitrant DOC were found in a forest brook draining a forest swamp. Concentrations of predominantly recalcitrant POC and DOC were low in a groundwater-fed forest spring. Overall, DOC concentration was two to 18 times higher than POC concentrations. 3. Oxygen consumption rate at 20 °C was higher during summer than winter, higher in open than shaded streams and higher in lake outlets than inlets. Rate was closely related to concentrations of chlorophyll and POC but not to DOC. The ratio of oxygen consumption rate to total organic concentrations (DOC + POC), serving as a measure of organic degradability, was highest downstream of lakes, intermediate in open streams and lowest in forest streams. 4. Temperature coefficients describing the exponential increase of oxygen consumption rate between 4 and 20 °C averaged 0.121 °C,1 (Q10 of 3.35) in 70 measurements and showed no significant variations between seasons and stream sites or correlations with ambient temperature and organic content. 5. Oxygen consumption rate was enhanced downstream of lakes during summer because of higher temperature and, more significantly, greater concentrations of degradable organic carbon. Oxygen consumption rates were up to seven times higher in the stream with three impoundments than in a neighbouring unshaded stream and 21 times higher than in the groundwater-fed forest spring. 6. A regional climate model has calculated a dramatic 4,5 °C rise in air temperature over Denmark by 2070,2100. If this is realised, unshaded streams are estimated to become 2,3 °C warmer in summer and winter and 5,7 °C warmer in spring and, thereby, increase oxygen consumption rates at ambient temperature by 30,40% and 80,130%, respectively. Faster consumption of organic matter and dissolved oxygen downstream of point sources should increase the likelihood of oxygen stress of the stream biota and lead to the export of less organic matter but more mineralised nutrients to the coastal waters. [source] The effect of some water parameters on the oxygen consumption rate of embryos and larvae of the Chinese Sturgeon (Acipenser sinensis)JOURNAL OF APPLIED ICHTHYOLOGY, Issue 2006Jian-Yi. First page of article [source] Antarctic fish can survive prolonged exposure to elevated temperaturesJOURNAL OF FISH BIOLOGY, Issue 7 2008E. Robinson The Antarctic notothenioid Pagothenia borchgrevinki was collected from the stenothermal waters of McMurdo Sound in the summers of 2004, 2005 and 2006. Acclimation ability at 4° C was tested in healthy P. borchgrevinki and in individuals infected with x-cell gill disease. All healthy fish successfully acclimated to 4° C, establishing compensatory changes in resting oxygen consumption rate (Rrest) and critical swimming speed (Ucrit) during a 1 month acclimation period, which were maintained during a longer, 6 month acclimation period. In contrast, individuals infected with x-cell disease were unable to acclimate to 4° C, demonstrating significantly reduced survival rates compared with healthy individuals at 4° C. Measurements of Rrest suggest that limitations in the ability of x-cell fish to uptake oxygen from the external milieu may have a negative effect on their survival at 4° C. [source] Comparison of Assays for Metmyoglobin Reducing Ability in Beef Inside and Outside Semimembranosus MuscleJOURNAL OF FOOD SCIENCE, Issue 3 2002L.M. Sammel ABSTRACT: The relationships of 6 assays for metmyoglobin (Metmb) reducing ability to color stability and the chemical differences between the inside (ISM) and outside (OSM) beef semimembranosus (SM) muscle after 5 or 14 d storage were investigated. The ISM had less (p < 0.05) color stability than the OSM regardless of time post mortem, and both muscle portions were more color-stable when stored for 5 d rather than 14 d. Among the assays, aerobic reducing ability correlated best with visual color scores (r =,0.58) and Metmb accumulation (r =,0.61) in the SM. The ISM had less reducing ability than the OSM, which can be attributed partially to lower oxygen consumption rate and NAD concentrations (p < 0.05). [source] Reactive Oxygen Species Scavenging Enzymes and Down-Adjustment of Metabolism Level in Mitochondria Associated with Desiccation-Tolerance Acquisition of Maize EmbryoJOURNAL OF INTEGRATIVE PLANT BIOLOGY, Issue 7 2009Jing-Hua Wu Abstract It is a well-known fact that a mature seed can survive losing most of its water, yet how seeds acquire desiccation-tolerance is not well understood. Through sampling maize embryos of different developmental stages and comparatively studying the integrity, oxygen consumption rate and activities of antioxidant enzymes in the mitochondria, the main origin site of reactive oxygen species (ROS) production in seed cells, we found that before an embryo achieves desiccation-tolerance, its mitochondria shows a more active metabolism, and might produce more ROS and therefore need a more effective ROS scavenging system. However, embryo dehydration in this developmental stage declined the activities of most main antioxidant enzymes and accumulated thiobarbituric acid-reactive products in mitochondria, and then destroyed the structure and functional integrity of mitochondria. In physiologically-matured embryos (dehydration-tolerant), mitochondria showed lower metabolism levels, and no decline in ROS scavenging enzyme activities and less accumulation of thiobarbituric acid-reactive products after embryo dehydration. These data indicate that seed desiccation-tolerance acquisition might be associated with down-adjustment of the metabolism level in the late development stage, resulting in less ROS production, and ROS scavenging enzymes becoming desiccation-tolerant and then ensuring the structure and functional integrity of mitochondria. [source] Cardiac 17O MRI: Toward direct quantification of myocardial oxygen consumptionMAGNETIC RESONANCE IN MEDICINE, Issue 6 2010Kyle S. McCommis Abstract A new 17O-labeled blood contrast agent was injected intravenously in control dogs. Electrocardiogram (ECG)-triggered myocardial T1, imaging was performed to obtain spin-locking T1,-weighted myocardial signals for the detection of resultant metabolite H217O water in the heart. Bolus and slow injection methods of various doses of the 17O-labeled and 16O-labeled agents were carried out in order to evaluate the sensitivity of this method and determine the optimal injection method. Bolus injection provided approximately 1% signal reduction, whereas slow injection with larger amount of agent yielded 11.9 ± 0.6% signal reduction. Myocardial oxygen consumption rate was determined by a technique to quantify cerebral oxygenation consumption rate previously developed in 17O brain studies. With either injection method, myocardial oxygen consumption rate at rest was 5.0 , 5.6 ,mol/g/min. Therefore, it appears feasible to detect metabolically generated HO water in vivo in the heart, using the 17O-labeled blood tracer. Myocardial oxygen consumption rate can then be quantified in vivo, which may open new doors for the assessment of myocardial metabolism. Magn Reson Med 63:1442,1447, 2010. © 2010 Wiley-Liss, Inc. [source] EPR oximetry in the beating heart: Myocardial oxygen consumption rate as an index of postischemic recoveryMAGNETIC RESONANCE IN MEDICINE, Issue 4 2004Govindasamy Ilangovan Abstract Oxygen plays a critical role in the pathophysiology of myocardial injury during both ischemia and subsequent reperfusion (I/R). Thus, oxygen concentration is an important variable to measure during I/R. In the present work, electron paramagnetic resonance (EPR)-based oximetry was used to measure the oxygen concentration during a series of I/R episodes and oxygenation levels were correlated with the contractile and hemodynamic functions of the heart. A custom-developed electronically tunable surface coil resonator working at 1.1 GHz was used to determine tissue pO2 in the beating heart. Microcrystalline particulate of lithium phthalocyanine was used as an EPR oximetry probe. Isolated and perfused rat hearts were subjected to 1 or 3 hr durations of preischemic perfusion, followed by 15-min I/R cycles. In hearts perfused for 3 hr prior to 15-min I/R cycles, the myocardial pO2 decreased gradually on subsequent reperfusions of three successive I/R cycles. However, in hearts perfused for 1 hr there was almost 100% recovery of myocardial pO2 in all three I/R cycles. The extent of oxygenation recovered in each reperfusion cycle correlated with the recovery of hemodynamic and contractile function. The results also showed that the oxygen consumption rate of the heart at the end of each I/R episode decreased in direct proportion to the functional recovery. In summary, it was observed that the amount of myocardial oxygen consumption during I/R could provide a reliable index of functional impairment in the heart. Magn Reson Med 51:835,842, 2004. © 2004 Wiley-Liss, Inc. [source] REDUCTIONS IN OXYGEN CONSUMPTION DURING DIVES AND ESTIMATED SUBMERGENCE LIMITATIONS OF STELLER SEA LIONS (EUMETOPIAS JUBATUS)MARINE MAMMAL SCIENCE, Issue 2 2007Gordon D. Hastie Abstract Accurate estimates of diving metabolic rate are central to assessing the energy needs of marine mammals. To circumvent some of the limitations inherent with conducting energy studies in both the wild and captivity, we measured diving oxygen consumption of two trained Steller sea lions (Eumetopias jubatus) in the open ocean. The animals dived to predetermined depths (5,30 m) for controlled periods of time (50,200 s). Rates of oxygen consumption were measured using open-circuit respirometry before and after each dive. Mean resting rates of oxygen consumption prior to the dives were 1.34 (±0.18) and 1.95 (±0.19) liter/min for individual sea lions. Mean rates of oxygen consumption during the dives were 0.71 (±0.24) and 1.10 (±0.39) liter/min, respectively. Overall, rates of oxygen consumption during dives were significantly lower (45% and 41%) than the corresponding rates measured before dives. These results provide the first estimates of diving oxygen consumption rate for Steller sea lions and show that this species can exhibit a marked decrease in oxygen consumption relative to surface rates while submerged. This has important consequences in the evaluation of physiological limitations associated with diving such as dive duration and subsequent interpretations of diving behavior in the wild. [source] Theoretical Simulation of Oxygen Transport to Brain by Networks of Microvessels: Effects of Oxygen Supply and Demand on Tissue HypoxiaMICROCIRCULATION, Issue 4 2000T.W. SECOMB ABSTRACT Objective: Simulations of oxygen delivery by a three-dimensional network of microvessels in rat cerebral cortex were used to examine how the distribution of partial pressure of oxygen (PO2) in tissue depends on blood flow and oxygen consumption rates. Methods: Network geometry was deduced from previously published scanning electron micrographs of corrosion casts. A nonlinear least-squares method, using images obtained at three different angles, was used to estimate vessel locations. The network consisted of 50 segments in a region 140 µm × 150 µm × 160 µm. A Green's function method was used to predict the PO2 distribution. Effects of varying perfusion and consumption were examined, relative to a control state with consumption 10 cm3O2/100 g per min and perfusion 160 cm3/100 g per min. Results: In the control state, minimum tissue PO2 was 7 mm Hg. A Krogh-type model with the same density of vessels, but with uniform spacing, predicted a minimum tissue PO2 of 23 mm Hg. For perfusion below 60% of control, tissue hypoxia (PO2 <1 mm Hg) was predicted. When perfusion was reduced by 75%, the resulting hypoxia could be eliminated by a 31% reduction in oxygen consumption rate. Conclusions: The simulations suggest that tissue hypoxia resulting from a severe decrease in brain perfusion, as can occur in stroke, may be avoided by a moderate decrease in oxygen consumption rate. [source] Quantifying oxygen diffusion in paraffin oil used in oocyte and embryo cultureMOLECULAR REPRODUCTION & DEVELOPMENT, Issue 12 2009Yvonne M. StokesArticle first published online: 11 AUG 200 Oxygen diffusion through oil is important in the culture of oocytes and embryos. A diffusion coefficient two orders of magnitude smaller than that of oxygen in water has been thought possible, and this has led to concerns of anoxia in cultures. Using an assay for determining the oxygen consumption rate of embryos and oocytes, along with a mathematical model, it is here shown that the oxygen diffusion rate in paraffin oil at 37°C is about two-thirds of that in water at the same temperature. Although not previously recognised for the assay in question, the geometry is such that anoxia does occur for a period of time in excess of 1,hr and, by the completion of the assay, 30,40% of the medium is anoxic. Hence the quantity of oxygen consumed is less than would be consumed in conditions of plentiful oxygen supply. Nevertheless, using a model with a concentration dependent oxygen consumption rate, the oxygen consumption rate can be estimated. Mol. Reprod. Dev. 76: 1178,1187, 2009. © 2009 Wiley-Liss, Inc. [source] Supplementary oxygen and temperature management during live transportation of greenlip abalone, Haliotis laevigata (Donovan, 1808)AQUACULTURE RESEARCH, Issue 7 2009Erin J Bubner Abstract Live greenlip abalone, Haliotis laevigata, are highly valued in Australian export markets with demand increasingly being met with cultured stock. Live transportation of abalone requires the maintenance of favourable conditions within transport containers for periods exceeding 35 h. We examined the combined effects of temperature regulation (ice provision) and of supplemental oxygen (60% and 100% concentrations) on mortality rates of abalone over 7 days following a 35-h simulated live-transport experiment. We also examined the physiological condition of greenlip abalone (oxygen consumption rate, haemolymph pH and weight) during the simulation experiment. The provision of ice and supplementary oxygen reduced abalone mortalities. Omission of ice and supplementary oxygen during the transport simulation resulted in mortality rates ranging from 70% to 100%. The addition of ice to containers with ambient oxygen concentrations decreased average mortality rates by 50%. While supplementary oxygen further reduced these rates, the provision of both ice and 100% oxygen was by far the most effective combination, reducing mortalities to between 2% and 6%. Supplementary oxygen increased oxygen consumption rates of abalone above those transported at ambient oxygen concentrations. Live-transport decreased haemolymph pH in all treatments but was most pronounced in treatments without ice or supplementary oxygen. On average, abalone lost 7,13% of their weight during the simulation but this loss was independent of transport treatment. [source] Effect of body size, photophase, feeding and emersion on the oxygen consumption of the east coast rock lobster Panulirus homarus rubellusAQUACULTURE RESEARCH, Issue 7 2009Justin O G Kemp Abstract An understanding of the effect of key intrinsic and extrinsic factors on the oxygen consumption of Panulirus homarus rubellus is imperative for the development of optimized transport, holding and growout protocols. This study investigated the effect of body weight, photophase, feeding and emersion on the oxygen consumption rate of P. h. rubellus. Body weight was positively correlated to both standard and active oxygen consumption (mg O2 h,1) while body weight was negatively correlated to mass-specific standard oxygen uptake (mg O2 g,1 h,1). Diurnal rhythm exhibited a strong effect on the lobsters' oxygen consumption, with average nighttime values 67% greater than those recorded during the day. This was related to activity driven by intrinsic nocturnal foraging behaviour. Feeding resulted in a classic specific dynamic action response, with postprandial oxygen consumption increasing to a peak before decreasing gradually to preprandial levels. Emersion caused a significant increase in oxygen consumption following re-immersion. Lobsters rapidly recovered to pre-emersion levels after 4 h following a 1-h emersion and after 13.3 h following an emersion period of 6 h. The implications of these results with regard to the holding/culture system and protocol design are discussed. [source] Stoichiometric model and metabolic flux analysis for Leptospirillum ferrooxidansBIOTECHNOLOGY & BIOENGINEERING, Issue 4 2010M.P. Merino Abstract A metabolic model for Leptospirillum ferrooxidans was developed based on the genomic information of an analogous iron oxidizing bacteria and on the pathways of ferrous iron oxidation, nitrogen and CO2 assimilation based on experimental evidence for L. ferrooxidans found in the literature. From this metabolic reconstruction, a stoichiometric model was built, which includes 86 reactions describing the main catabolic and anabolic aspects of its metabolism. The model obtained has 2 degrees of freedom, so two external fluxes were estimated to achieve a determined and observable system. By using the external oxygen consumption rate and the generation flux biomass as input data, a metabolic flux map with a distribution of internal fluxes was obtained. The results obtained were verified with experimental data from the literature, achieving a very good prediction of the metabolic behavior of this bacterium at steady state. Biotechnol. Bioeng. 2010;107:696,706. © 2010 Wiley Periodicals, Inc. [source] Limitation of oxygenic photosynthesis and oxygen consumption by phosphate and organic nitrogen in a hypersaline microbial mat: a microsensor studyFEMS MICROBIOLOGY ECOLOGY, Issue 1 2006Rebecca Ludwig Abstract Microbial mats are characterized by high primary production but low growth rates, pointing to a limitation of growth by the lack of nutrients or substrates. We identified compounds that instantaneously stimulated photosynthesis rates and oxygen consumption rates in a hypersaline microbial mat by following the short-term response (c. 6 h) of these processes to addition of nutrients, organic and inorganic carbon compounds, using microsensors. Net photosynthesis rates were not stimulated by compound additions. However, both gross photosynthesis and oxygen consumption were substantially stimulated (by a minimum of 25%) by alanine (1 mM) and glutamate (3.5 mM) as well as by phosphate (0.1 mM). A low concentration of ammonium (0.1 mM) did not affect photosynthesis and oxygen consumption, whereas a higher concentration (3.5 mM) decreased both process rates. High concentrations of glycolate (5 mM) and phosphate (1 mM) inhibited gross photosynthesis but not oxygen consumption, leading to a decrease of net photosynthesis. Photosynthesis was not stimulated by addition of inorganic carbon, nor was oxygen consumption stimulated by organic compounds like glycolate (5 mM) or glucose (5 mM), indicating that carbon was efficiently cycled within the mat. Photosynthesis and oxygen consumption were apparently tightly coupled, because stimulations always affected both processes to the same extent, which resulted in unchanged net photosynthesis rates. These findings illustrate that microsensor techniques, due to their ability to quantify all three processes, can clarify community responses to nutrient enrichment studies much better than techniques that solely monitor net fluxes. [source] Differences in temperature, organic carbon and oxygen consumption among lowland streamsFRESHWATER BIOLOGY, Issue 12 2005KAJ SAND-JENSEN Summary 1. Temperature, organic carbon and oxygen consumption were measured over a year at 13 sites in four lowlands streams within the same region in North Zealand, Denmark with the objectives of determining: (i) spatial and seasonal differences between open streams, forest streams and streams with or without lakes, (ii) factors influencing the temperature dependence of oxygen consumption rate, (iii) consequences of higher temperature and organic content in lake outlets on oxygen consumption rate, and (iv) possible consequences of forecasted global warming on degradation of organic matter. 2. High concentrations of easily degradable dissolved (DOC) and particulate organic carbon (POC) were found in open streams downstream of plankton-rich lakes, while high concentrations of recalcitrant DOC were found in a forest brook draining a forest swamp. Concentrations of predominantly recalcitrant POC and DOC were low in a groundwater-fed forest spring. Overall, DOC concentration was two to 18 times higher than POC concentrations. 3. Oxygen consumption rate at 20 °C was higher during summer than winter, higher in open than shaded streams and higher in lake outlets than inlets. Rate was closely related to concentrations of chlorophyll and POC but not to DOC. The ratio of oxygen consumption rate to total organic concentrations (DOC + POC), serving as a measure of organic degradability, was highest downstream of lakes, intermediate in open streams and lowest in forest streams. 4. Temperature coefficients describing the exponential increase of oxygen consumption rate between 4 and 20 °C averaged 0.121 °C,1 (Q10 of 3.35) in 70 measurements and showed no significant variations between seasons and stream sites or correlations with ambient temperature and organic content. 5. Oxygen consumption rate was enhanced downstream of lakes during summer because of higher temperature and, more significantly, greater concentrations of degradable organic carbon. Oxygen consumption rates were up to seven times higher in the stream with three impoundments than in a neighbouring unshaded stream and 21 times higher than in the groundwater-fed forest spring. 6. A regional climate model has calculated a dramatic 4,5 °C rise in air temperature over Denmark by 2070,2100. If this is realised, unshaded streams are estimated to become 2,3 °C warmer in summer and winter and 5,7 °C warmer in spring and, thereby, increase oxygen consumption rates at ambient temperature by 30,40% and 80,130%, respectively. Faster consumption of organic matter and dissolved oxygen downstream of point sources should increase the likelihood of oxygen stress of the stream biota and lead to the export of less organic matter but more mineralised nutrients to the coastal waters. [source] Mitochondrial function and apoptotic susceptibility in aging skeletal muscleAGING CELL, Issue 1 2008Béatrice Chabi Summary During aging, skeletal muscle undergoes sarcopenia, a condition characterized by a loss of muscle cell mass and alterations in contractile function. The origin of these decrements is unknown, but evidence suggests that they can be partly attributed to mitochondrial dysfunction. To characterize the nature of this dysfunction, we investigated skeletal muscle contractile properties, subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondrial biogenesis and function, as well as apoptotic susceptibility in young (6 months old) and senescent (36 months old) Fischer 344 Brown Norway rats. Muscle mass and maximal force production were significantly lower in the 36-month group, which is indicative of a sarcopenic phenotype. Furthermore, contractile activity in situ revealed greater fatigability in the 36-month compared to the 6-month animals. This decrement could be partially accounted for by a 30% lower mitochondrial content in fast-twitch muscle from 36-month animals, as well as lower protein levels of the transcriptional coactivator peroxisome proliferator-activated receptor , coactivator-1,. Enzyme activities and glutamate-induced oxygen consumption rates in isolated SS and IMF mitochondria were similar between age groups. However, mitochondrial reactive oxygen species (ROS) production during state 3 respiration was ~1.7-fold greater in mitochondria isolated from 36-month compared to 6-month animals, and was accompanied by a 1.8-fold increase in the DNA repair enzyme 8-oxoguanine glycosylase 1 in fast-twitch muscle. Basal rates of release of cytochrome c and endonuclease G in SS mitochondria were 3.5- to 7-fold higher from senescent animals. These data suggest that the age-related sarcopenia and muscle fatigability are associated with enhanced ROS production, increased mitochondrial apoptotic susceptibility and reduced transcriptional drive for mitochondrial biogenesis. [source] IMPACT OF IRON LIMITATION ON THE PHOTOSYNTHETIC APPARATUS OF THE DIATOM CHAETOCEROS MUELLERI (BACILLARIOPHYCEAE)JOURNAL OF PHYCOLOGY, Issue 6 2001Margaret Davey Iron starvation induced marked increases in flavodoxin abundance and decreases in light-saturated and light-limited photosynthesis rates in the diatom Chaetoceros muelleri. Consistent with the substitution of flavodoxin for ferredoxin as an early response to iron starvation, increases of flavodoxin abundance were observed before declines of cell division rate or chl a specific photosynthesis rates. Changes in the abundance of flavodoxin after the addition of iron to iron-starved cells indicated that flavodoxin was not actively degraded under iron-replete conditions. Greater declines in light-saturated oxygen evolution rates than dark oxygen consumption rates indicated that the mitochondrial electron transfer chain was not affected as greatly by iron starvation as the photosynthetic electron transfer chain. The carbon:nitrogen ratio was unaffected by iron starvation, suggesting that photosynthetic electron transfer was a primary target of iron starvation and that reductions in nitrate assimilation were due to energy limitation (the C:N ratio would be expected to rise under nitrogen-limited but energy-replete conditions). Parallel changes were observed in the maximum light-saturated photosynthesis rate and the light-limited initial slope of the photosynthesis-light curve during iron starvation and recovery. The lowest photosynthesis rates were observed in iron-starved cells and the highest values in iron-replete cells. The light saturation parameter, Ik, was not affected by iron starvation, nor was the chl-to-C ratio markedly reduced. These observations were consistent with iron starvation having a similar or greater effect on photochemical charge separation in PSII than on downstream electron transfer steps. Declines of the ratio of variable to maximum fluorescence in iron-starved cells were consistent with PSII being a primary target of iron starvation. The functional cross-section of PSII was affected only marginally (<20%) by iron starvation, with the largest values observed in iron-starved cells. The rate constant for electron transfer calculated from fast repetition rate fluorescence was found to covary with the light-saturated photosynthesis rate; it was lowest in the most severely starved cells. [source] Theoretical Simulation of Oxygen Transport to Brain by Networks of Microvessels: Effects of Oxygen Supply and Demand on Tissue HypoxiaMICROCIRCULATION, Issue 4 2000T.W. SECOMB ABSTRACT Objective: Simulations of oxygen delivery by a three-dimensional network of microvessels in rat cerebral cortex were used to examine how the distribution of partial pressure of oxygen (PO2) in tissue depends on blood flow and oxygen consumption rates. Methods: Network geometry was deduced from previously published scanning electron micrographs of corrosion casts. A nonlinear least-squares method, using images obtained at three different angles, was used to estimate vessel locations. The network consisted of 50 segments in a region 140 µm × 150 µm × 160 µm. A Green's function method was used to predict the PO2 distribution. Effects of varying perfusion and consumption were examined, relative to a control state with consumption 10 cm3O2/100 g per min and perfusion 160 cm3/100 g per min. Results: In the control state, minimum tissue PO2 was 7 mm Hg. A Krogh-type model with the same density of vessels, but with uniform spacing, predicted a minimum tissue PO2 of 23 mm Hg. For perfusion below 60% of control, tissue hypoxia (PO2 <1 mm Hg) was predicted. When perfusion was reduced by 75%, the resulting hypoxia could be eliminated by a 31% reduction in oxygen consumption rate. Conclusions: The simulations suggest that tissue hypoxia resulting from a severe decrease in brain perfusion, as can occur in stroke, may be avoided by a moderate decrease in oxygen consumption rate. [source] Effect of salinity on survival, growth, oxygen consumption and ammonia-N excretion of juvenile whiteleg shrimp, Litopenaeus vannameiAQUACULTURE RESEARCH, Issue 12 2009Peidong Zhang Abstract In this study, we tested the lower salinity tolerance of juvenile shrimps (Litopenaeus vannamei) at a relatively low temperature (20 °C). In the first of two laboratory experiments, we first abruptly transferred shrimps (6.91 ± 0.05 g wet weight, mean ± SE) from the rearing salinity (35 000 mg L,1) to salinities of 5000, 15 000, 25 000, 35 000 (control) and 40 000 mg L,1 at 20 °C. The survival of L. vannamei juvenile was not affected by salinities from 15 000 to 40 000 mg L,1 during the 96-h exposure periods. Shrimps exposed to 5000 mg L,1 were significantly affected by salinity, with a survival of 12.5% after 96 h. The 24-, 48- and 96-h lethal salinity for 50% (LS50) were 7020, 8510 and 9540 mg L,1 respectively. In the second experiment, shrimps (5.47 ± 0.09 g wet weight, mean ± SE) were acclimatized to the different salinity levels (5000, 15 000, 25 000, 35 000 and 40 000 mg L,1) and then maintained for 30 days at 20 °C. Results showed that the survival was significantly lower at 5000 mg L,1 than at other salinity levels, but the final wet weight under 5000 mg L,1 treatment was significantly higher than those under other treatments (P<0.05). Feed intake (FI) of shrimp under 5000 mg L,1 was significantly lower than those of shrimp under 150 00,40 000 mg L,1; food conversion efficiency (FCE), however, showed a contrasting change (P<0.05). Furthermore, salinity significantly influenced the oxygen consumption rates, ammonia-N excretion rates and the O/N ratio of test shrimps (P<0.05). The results obtained in our work provide evidence that L. vannamei juveniles have limited capacity to tolerate salinities <10 000 mg L,1 at a relatively low temperature (20 °C). Results also show that L. vannamei juvenile can recover from the abrupt salinity change between 15 000 and 40 000 mg L,1 within 24 h. [source] Supplementary oxygen and temperature management during live transportation of greenlip abalone, Haliotis laevigata (Donovan, 1808)AQUACULTURE RESEARCH, Issue 7 2009Erin J Bubner Abstract Live greenlip abalone, Haliotis laevigata, are highly valued in Australian export markets with demand increasingly being met with cultured stock. Live transportation of abalone requires the maintenance of favourable conditions within transport containers for periods exceeding 35 h. We examined the combined effects of temperature regulation (ice provision) and of supplemental oxygen (60% and 100% concentrations) on mortality rates of abalone over 7 days following a 35-h simulated live-transport experiment. We also examined the physiological condition of greenlip abalone (oxygen consumption rate, haemolymph pH and weight) during the simulation experiment. The provision of ice and supplementary oxygen reduced abalone mortalities. Omission of ice and supplementary oxygen during the transport simulation resulted in mortality rates ranging from 70% to 100%. The addition of ice to containers with ambient oxygen concentrations decreased average mortality rates by 50%. While supplementary oxygen further reduced these rates, the provision of both ice and 100% oxygen was by far the most effective combination, reducing mortalities to between 2% and 6%. Supplementary oxygen increased oxygen consumption rates of abalone above those transported at ambient oxygen concentrations. Live-transport decreased haemolymph pH in all treatments but was most pronounced in treatments without ice or supplementary oxygen. On average, abalone lost 7,13% of their weight during the simulation but this loss was independent of transport treatment. [source] Characterization of a Hollow Fiber Bioartificial Liver DeviceARTIFICIAL ORGANS, Issue 5 2005Susan Fugett Abu-Absi Abstract:, A three-compartment bioartificial liver (BAL) has been developed for potential treatment of fulminant hepatic failure. It has been shown previously that viability and liver-specific functions were maintained in laboratory-scale bioreactors of such design. In this study, the performance of hepatocytes in a clinical-scale bioartificial liver was verified by sustained specific production rates of albumin and urea, along with oxygen consumption rates for up to 56 h and liver-specific gene expression for up to 72 h. In addition, transmission of porcine endogenous retrovirus and other type C retroviral particles across the hollow fibers was not detected under both normal and extreme operating fluxes. These results demonstrate that the clinical-scale BAL performs at a level similar to the laboratory scale ,and, that, it, offers, a, viral, barrier, against, porcine , retroviruses. [source] Method for determining oxygen consumption rates of static cultures from microplate measurements of pericellular dissolved oxygen concentrationBIOTECHNOLOGY & BIOENGINEERING, Issue 3 2005Richard D. Guarino The original article to which this Erratum refers was published in Biotechnol Bioeng 2004;86:775,787 [source] Axial Distribution of Oxygen Concentration in Different Airlift Bioreactor Scales: Mathematical Modeling and SimulationCHEMICAL ENGINEERING & TECHNOLOGY (CET), Issue 9 2006H. Znad Abstract Steady and unsteady state oxygen concentration distributions in the liquid and gas phases along the axial direction of different airlift bioreactor scales have been simulated for various gas flow rates and oxygen consumption rates by applying the axial dispersion model to the riser and the downcomer, and a complete mixing model for the top (separator) and the bottom sections of the bioreactor. The results show that the dissolved oxygen concentration is very low at the lower part of the downcomer when the rate of oxygen consumption by microorganisms is very high. Furthermore, the shorter (small) bioreactor shows relatively more uniform axial dissolved oxygen concentrations than the longer (large) bioreactor, due to the effect of the hydrostatic pressure along the bioreactor. One of the most important geometric factors for mass transfer is the reactor height, which dominates the mean pressure and thus influences the saturation concentration and mass transfer driving force. The presented model can be applied for modeling and scale-up of practical airlift bioreactors. [source] | |||||||||||||||||||