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Mucosa Equivalents (mucosa + equivalent)

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Medical Sciences100%

Selected Abstracts

Skin and oral mucosa equivalents: construction and performance

ORTHODONTICS & CRANIOFACIAL RESEARCH, Issue 1 2010
J Liu
To cite this article: Liu J, Bian Z, Kuijpers-Jagtman AM, Von den Hoff JW: Skin and oral mucosa equivalents: construction and performance Orthod Craniofac Res 2010;13:11,20 Abstract Authors,,, Liu J, Bian Z, Kuijpers-Jagtman AM, Von den Hoff JW The skin and the oral mucosa act as a barrier against the external environment. Loss of this barrier function causes dehydration and a high risk of infection. For the treatment of extensive skin wounds such as in severe burns, autologous skin for transplantation is often not available in sufficient amounts. Reconstructions in the oral cavity, as required after tumor resections or cleft palate repair, are often complicated by similar problems. In the last two decades, the field of tissue engineering has provided new solutions to these problems. Techniques have been developed for the culture of epithelial grafts, dermal substitutes, and the combination of these two to a ,functional' skin or mucosa equivalent. The present review focuses on developments in the field of tissue engineering of skin and oral mucosa. The performance of different types of engineered grafts in animal models and clinical studies is discussed. Recent developments such as the use of epithelial stem cells, and gene therapy with transduced skin grafts are also discussed. [source]

Elastin in oral connective tissue modulates the keratinization of overlying epithelium

JOURNAL OF CLINICAL PERIODONTOLOGY, Issue 8 2010
Po-Chen Hsieh
Hsieh P-C, Jin Y-T, Chang C-W, Huang C-C, Liao S-C, Yuan K. Elastin in oral connective tissue modulates the keratinization of overlying epithelium. J Clin Periodontol 2010; 37: 705-711 doi: 10.1111/j.1600-051X.2010.01542.x Abstract Aim: One of the most discernable differences between keratinized and non-keratinized oral mucosas is the quantity of elastin they contain in the connective tissues. Whether elastin modulates the keratin expression of oral epithelial cells is unknown. Methods: Four specimens containing both keratinized and non-keratinized mucosas were processed for immunohistochemical (IHC) stainings for elastin and four keratins. Six keratinized and non-keratinized portions of oral mucosas were dissected and cultured on an organ culture system. Purified elastin and elastase were added separately to the media. After 14 days, the mucosas were examined for four keratin expressions. Cell cultures of keratinized and non-keratinized gingival fibroblasts were established and tested for elastin expression. Oral mucosa equivalents were then engineered and tested for keratin expression. Results: Keratinized epithelium exclusively expressed keratin-1 and -10 (K1/10), while non-keratinized epithelium expressed keratin-4 and -13 (K4/13). Only non-keratinized fibroblasts expressed elastin in cell culture. Both the native and the engineered keratinized gingiva changed phenotypes and expressed K4/13 when treated with exogenous elastin. On the contrary, the native non-keratinized mucosa started to express K1/10 when elastase eradicated inherent elastin. Conclusions: Our study demonstrated that the elastin in the oral connective tissue is important for the non-keratinized phenotypes of overlaying epithelium. [source]

Skin and oral mucosa equivalents: construction and performance

ORTHODONTICS & CRANIOFACIAL RESEARCH, Issue 1 2010
J Liu
To cite this article: Liu J, Bian Z, Kuijpers-Jagtman AM, Von den Hoff JW: Skin and oral mucosa equivalents: construction and performance Orthod Craniofac Res 2010;13:11,20 Abstract Authors,,, Liu J, Bian Z, Kuijpers-Jagtman AM, Von den Hoff JW The skin and the oral mucosa act as a barrier against the external environment. Loss of this barrier function causes dehydration and a high risk of infection. For the treatment of extensive skin wounds such as in severe burns, autologous skin for transplantation is often not available in sufficient amounts. Reconstructions in the oral cavity, as required after tumor resections or cleft palate repair, are often complicated by similar problems. In the last two decades, the field of tissue engineering has provided new solutions to these problems. Techniques have been developed for the culture of epithelial grafts, dermal substitutes, and the combination of these two to a ,functional' skin or mucosa equivalent. The present review focuses on developments in the field of tissue engineering of skin and oral mucosa. The performance of different types of engineered grafts in animal models and clinical studies is discussed. Recent developments such as the use of epithelial stem cells, and gene therapy with transduced skin grafts are also discussed. [source]