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Appropriate Level (appropriate + level)
Selected AbstractsIs there a role for thyroid hormone on spermatogenesis?MICROSCOPY RESEARCH AND TECHNIQUE, Issue 11 2009Marcia Santos Wagner Abstract Appropriate level of thyroid hormone is essential for normal development and metabolism in most vertebrate tissues and altered thyroid status impacts adversely on them. For many years the testis was regarded as a thyroid hormone unresponsive organ, but consistent evidence accumulated in the past two decades has definitively changed this classical view. Currently, the concept that thyroid hormone plays a critical role in testis development, in rats and other vertebrate species, is clearly established. Although the effects of thyroid hormone on Sertoli and Leydig cells in the immature testis are well described, its role on the adult organ remains controversial. In this review, we summarize and discuss the recent development on the thyroid hormone effects in immature and adult testes. Particularly, we have attempted to address the role of thyroid hormone in the regulation of spermatogenesis, emphasizing recent data that suggest its involvement in germ cells differentiation and survival. Microsc. Res. Tech. 2009. © 2009 Wiley-Liss, Inc. [source] Waterfall attractions in coastal tourist areas: the Yorkshire coast and Queensland's Gold Coast comparedINTERNATIONAL JOURNAL OF TOURISM RESEARCH, Issue 4 2003Brian J. Hudson Abstract A comparative study of tourism development on the Yorkshire coast and Queensland's Gold Coast illustrates the importance of waterfalls as attractions, even in beach orientated tourist areas catering mainly for seaside holidaymakers. A relative decline in the importance of the Yorkshire coast waterfall attractions whereas those of the Gold Coast hinterland continue to play a major role is attributed to differences in quantity and quality of these scenic resources, problems of access and the availability of other, and, for many, superior, waterfall attractions within easy reach. Appropriate levels of development at and access to waterfall sites are discussed. Copyright © 2003 John Wiley &Sons, Ltd. [source] DEFINING STANDARD OF CARE IN THE DEVELOPING WORLD: THE INTERSECTION OF INTERNATIONAL RESEARCH ETHICS AND HEALTH SYSTEMS ANALYSISDEVELOPING WORLD BIOETHICS, Issue 2 2005ADNAN A. HYDER ABSTRACT In recent years there has been intense debate regarding the level of medical care provided to ,standard care' control groups in clinical trials in developing countries, particularly when the research sponsors come from wealthier countries. The debate revolves around the issue of how to define a standard of medical care in a country in which many people are not receiving the best methods of medical care available in other settings. In this paper, we argue that additional dimensions of the standard of care have been hitherto neglected, namely, the structure and efficiency of the national health system. The health system affects locally available medical care in two important ways: first, the system may be structured to provide different levels of care at different sites with referral mechanisms to direct patients to the appropriate level of care. Second, inefficiencies in this system may influence what care is available in a particular locale. As a result of these two factors locally available care cannot be equated with a national ,standard'. A reasonable approach is to define the national standard of care as the level of care that ought to be delivered under conditions of appropriate and efficient referral in a national system. This standard is the minimum level of care that ought to be provided to a control group. There may be additional moral arguments for higher levels of care in some circumstances. This health system analysis may be helpful to researchers and ethics committees in designing and reviewing research involving standard care control groups in developing country research. [source] Schizophrenia treatment: content versus deliveryACTA PSYCHIATRICA SCANDINAVICA, Issue 2009J. Van Os Objective:, To review the evidence supporting the importance of ensuring that patients with psychiatric disorders receive an optimal and appropriate level of non-pharmacological treatment, and how Assertive Community Treatment (ACT) may be able to contribute to this aim. Method:, Analysis of data from selected individual published studies on ACT, in addition to reviews from the Cochrane Library, and other study groups. Results:, Treatment management using ACT appears to offer benefits in terms of reduction in hospitalisation, although there is some debate as to whether this is the most representative outcome measure. Preliminary indications using remission as an outcome measure have also shown promising results in favour of ACT. Conclusion:, While further investigation and validation are necessary, current data indicate that ACT may be an appropriate strategy to facilitate the delivery of treatment to patients with psychotic disorders. [source] Behavioural Syndromes in Urban and Rural Populations of Song SparrowsETHOLOGY, Issue 7 2010Jackson Evans Animals in urban habitats are often noticeably bold in the presence of humans. Such boldness may arise due to habituation, as urban animals learn, through repeated exposure, that passing humans do not represent a threat. However, there is growing research suggesting that: (1) inherent traits, as opposed to learned behaviour, influence which species invade urban habitats, and (2) individuals exhibit individual personality traits that limit behavioural flexibility, with the possible result that not all individuals would be able to demonstrate an appropriate level of boldness in urban environments. As a result, perhaps only birds with inherently bold personalities could successfully settle in an area of high human disturbance, and further, we might also expect to see the existence of behavioural syndromes, where boldness is correlated with variation in other behavioural traits such as aggression. In this study, we examined boldness and territorial aggression in urban and rural populations of song sparrows. We found that urban birds were bolder towards humans and that urban birds also showed higher levels of territorial aggression. We also found an overall correlation between boldness and territorial aggression, suggesting that urban boldness may be part of a behavioural syndrome. However, we see no correlation between boldness and aggression in the urban population, and thus, more work is needed to determine the mechanisms accounting for high levels of boldness and aggression urban song sparrows. [source] Policy analysis for tropical marine reserves: challenges and directionsFISH AND FISHERIES, Issue 1 2003Murray A Rudd Abstract Marine reserves are considered to be a central tool for marine ecosystem-based management in tropical inshore fisheries. The arguments supporting marine reserves are often based on both the nonmarket values of ecological amenities marine reserves provide and the pragmatic cost-saving advantages relating to reserve monitoring and enforcement. Marine reserves are, however, only one of a suite of possible policy options that might be used to achieve conservation and fisheries management objectives, and have rarely been the focus of rigorous policy analyses that consider a full range of economic costs and benefits, including the transaction costs of management. If credible analyses are not undertaken, there is a danger that current enthusiasm for marine reserves may wane as economic performance fails to meet presumed potential. Fully accounting for the value of ecological services flowing from marine reserves requires consideration of increased size and abundance of focal species within reserve boundaries, emigration of target species from reserves to adjacent fishing grounds, changes in ecological resilience, and behavioural responses of fishers to spatially explicit closures. Expanding policy assessments beyond standard cost,benefit analysis (CBA) also requires considering the impact of social capital on the costs of managing fisheries. In the short term, the amount of social capital that communities possess and the capacity of the state to support the rights of individuals and communities will affect the relative efficiency of marine reserves. Reserves may be the most efficient policy option when both community and state capacity is high, but may not be when one and/or the other is weak. In the longer term, the level of social capital that a society possesses and the level of uncertainty in ecological and social systems will also impact the appropriate level of devolution or decentralization of fisheries governance. Determining the proper balance of the state and the community in tropical fisheries governance will require broad comparative studies of marine reserves and alternative policy tools. [source] Establishing a minimum standard for collaborative research in federal environmental agencies,INTEGRATED ENVIRONMENTAL ASSESSMENT AND MANAGEMENT, Issue 3 2008Kalle E Matso Abstract There is a general consensus that,given the magnitude of the challenges facing our nation's natural resource managers,the rate, efficiency, and effectiveness of linking research to decision making must be enhanced. Many reports have touched on this issue, most of them culminating with the exhortation to "foster more interactions between scientists and users," but very few documents provide details or assign responsibility to drive the interactions that most agree should happen. As a result, many natural science and engineering programs "talk the talk",that is, they say they do collaborative research with intended users; however, upon inspection, few of them "walk the walk" by effectively supporting collaboration throughout the research process. Moreover, when called to support transition to application in specific ways, research agencies often balk, most often objecting that research programs cannot afford to take any support away from funding more research. They may also argue that science works best for society when it is freed from concerns related to application. In this paper we will 1) review the cultural conflict that often underlies disagreements about collaborative research, 2) offer details on the basic ingredients required to achieve a minimum standard for collaborative research, 3) suggest an approach for determining the appropriate level of support for collaborative research, given various research goals, and 4) recommend specific steps for motivating scientists and stakeholders to participate in collaborative research. [source] Bank Mergers in Europe: The Public Policy IssuesJCMS: JOURNAL OF COMMON MARKET STUDIES, Issue 3 2000Jean Dermine A very large merger wave in the banking industry has taken place in Europe over the last 15 years. Public policy-makers need to assess how bank mergers , be they domestic intra-industry, across-industry, or cross-border , affect their mission of protecting investors and ensuring financial stability, an appropriate level of competition, and the competitiveness of national firms. Moreover, as the banking world is becoming increasingly international, there is a need to reassess the structure of bank regulation and supervision which has been assumed historically by each nation-state. [source] Ecosystem science and human,environment interactions in the Hawaiian archipelagoJOURNAL OF ECOLOGY, Issue 3 2006PETER VITOUSEK Summary 1Tansley's ecosystem concept remains a vital framework for ecological research in part because the approach facilitates interdisciplinary analyses of ecological systems. 2Features of the Hawaiian Islands , particularly the nearly orthogonal variation in many of the factors that control variation among ecosystems elsewhere , make the archipelago a useful model system for interdisciplinary research designed to understand fundamental controls on the state and dynamics of ecosystems, and their consequences for human societies. 3Analyses of rain forest sites arrayed on a substrate age gradient from c. 300 years to over 4 million years across the Hawaiian archipelago demonstrate that the sources of calcium and other essential cations shift from > 80% rock-derived in young sites to > 80% derived from marine aerosol on substrates older than 100 000 years. Rock-derived phosphorus is retained longer within ecosystems, but eventually long-distance transport of continental dust from Asia becomes the most important source of phosphorus. 4A biogeochemical feedback from low nutrient availability to efficient resource use by trees to slow decomposition and nutrient regeneration accentuates the geochemically driven pattern of low phosphorus availability and phosphorus limitation to net primary productivity in the oldest site. 5Variations in ecosystem biogeochemistry across the archipelago shaped the development and sustainability of Polynesian agricultural systems in the millennium between their discovery of Hawai'i and contact by Europeans. Irrigated pondfields were largely confined to stream valleys on the older islands, while rain-fed dryland systems occupied a narrow zone of fertile, well-watered soils on the younger islands. 6The ecosystem approach often represents the most appropriate level of organization for analyses of human influences on ecological systems; it can play a central role in the design and analysis of alternative agricultural, industrial and residential systems that could reduce the human footprint on the Earth. [source] Multiresolution analysis on identification and dynamics of clusters in a circulating fluidized bedAICHE JOURNAL, Issue 3 2009Tung-Yu Yang Abstract A new wavelet-threshold criterion was developed to distinguish the cluster and the void phases from the transient solids holdup/concentration fluctuation signals when measured in a 108 mm-i.d. × 5.75 m-high circulating fluidized bed with FCC particles (dp = 78 ,m, ,p = 1,880 kg/m3). An appropriate level of approximation subsignal was systematically specified as a threshold for cluster identification, based on multiresolution analysis (MRA) of wavelet transformation. By the established threshold, the dynamic properties of clusters including the appearance time fraction of clusters Fcl, average cluster duration time ,cl, cluster frequency fcl, and local average solids holdup in clusters ,sc, at different radial and axial positions were determined under the turbulent, transition and fast fluidization flow regimes. The results also describe the dynamic properties of clusters and flow patterns in the splash zone along with the dense bottom region of the circulating fluidized beds. © 2009 American Institute of Chemical Engineers AIChE J, 2009 [source] Outsiders at the Bank of England's MPCJOURNAL OF MONEY, CREDIT AND BANKING, Issue 6 2009PETRA GERLACH-KRISTEN monetary policy committees; Bank of England; outsiders; recession aversion The monetary policy committee (MPC) of the Bank of England consists of five internal and four external members. We study the voting record and show that outsiders dissent more often than insiders and tend to prefer lower rates, especially during economic downturns. Moreover, dissents by outsiders help forecast future interest rate changes, in contrast to dissents by insiders. A model in which outsiders in contrast to insiders are "recession averse" and more uncertain regarding the appropriate level of interest rates replicates the observed voting pattern well. [source] IMPROVEMENT OF PHYSICAL PROPERTIES OF NONFAT FERMENTED MILK DRINK BY USING WHEY PROTEIN CONCENTRATEJOURNAL OF TEXTURE STUDIES, Issue 3 2009ASLI E. OZEN ABSTRACT The use of whey protein concentrate (WPC) for the improvement of physical properties of nonfat fermented milk drink was investigated. Drinks were prepared from nonfat milk powder and WPC at different proportions. Rheological properties, serum separation and particle size of the drinks were measured. The effect of WPC on the physical properties of the drinks was evaluated by comparison with those of commonly used stabilizers, including propylene glycol alginate and locust bean gum. WPC addition caused an increase in the consistency coefficient and thixotropy and a decrease in the particle size of the samples. There was no serum separation in the sample with 2% WPC. Large unstable aggregates were observed in the sample with 3% WPC, which also exhibited the highest serum separation. WPC up to a level of 2% positively influenced the physical properties of nonfat fermented milk drink similar to stabilizers. PRACTICAL APPLICATIONS Fermented milk drinks are consumed especially for their beneficial health effects. Physical properties of fermented milk drinks influence their quality and consumer acceptability. Hydrocolloid stabilizers are used for the improvement of physical properties of fermented milk products. Whey protein concentrates (WPC) with high protein content can be used to substitute hydrocolloid stabilizers. In this study, the effect of the addition of WPC with 75% protein in place of a part of the nonfat milk powder on the physical properties of nonfat fermented milk drink with 6% dry matter was investigated. Use of an appropriate level of WPC was found to be important for obtaining a desirable effect on the physical properties of nonfat fermented milk drink. The effect of WPC was found to be comparable to those of commonly used hydrocolloid stabilizers. Use of WPC also enhances the nutritional value of the product as whey proteins have a high biological value. [source] Non-empirical calculations of NMR indirect carbon,carbon coupling constants.MAGNETIC RESONANCE IN CHEMISTRY, Issue 8 2004Monocycloalkanes, Part Abstract Carbon,carbon and carbon,hydrogen spin,spin coupling constants were calculated in the series of the first six monocycloalkanes using SOPPA and SOPPA(CCSD) methods, and very good agreement with the available experimental data was achieved, with the latter method showing slightly better results in most cases, at least in those involving calculations of J(C,C). Benchmark calculations of all possible 21 coupling constants J(C,C), J(C,H) and J(H,H) in chair cyclohexane revealed the importance of using the appropriate level of theory and adequate quality of the basis sets. Many unknown couplings in this series were predicted with high confidence and several interesting structural trends (hybridization effects, multipath coupling transmission mechanisms, hyperconjugative interactions) were elucidated and are discussed based on the present calculations of spin,spin couplings. Copyright © 2004 John Wiley & Sons, Ltd. [source] Design principles for virtual patients: a focus group study among studentsMEDICAL EDUCATION, Issue 6 2009Sören Huwendiek Objectives, This study aimed to examine what students perceive as the ideal features of virtual patient (VP) design in order to foster learning with a special focus on clinical reasoning. Methods, A total of 104 Year 5 medical students worked through at least eight VPs representing four different designs during their paediatric clerkship. The VPs were presented in two modes and differed in terms of the authenticity of the user interface (with or without graphics support), predominant question type (long- versus short-menu questions) and freedom of navigation (relatively free versus predetermined). Each mode was presented in a rich and a poor version with regard to the use of different media and questions and explanations explicitly directed at clinical reasoning. Five groups of between four and nine randomly selected students (n = 27) participated in focus group interviews facilitated by a moderator using a questioning route. The interviews were videotaped, transcribed and analysed. Summary reports were approved by the students. Results, Ten principles of VP design emerged from the analysis. A VP should be relevant, of an appropriate level of difficulty, highly interactive, offer specific feedback, make optimal use of media, help students focus on relevant learning points, offer recapitulation of key learning points, provide an authentic web-based interface and student tasks, and contain questions and explanations tailored to the clinical reasoning process. Conclusions, Students perceived the design principles identified as being conducive to their learning. Many of these principles are supported by the results of other published studies. Future studies should address the effects of these principles using quantitative controlled designs. [source] On testing predictions of species relative abundance from maximum entropy optimisationOIKOS, Issue 4 2010Stephen H. Roxburgh A randomisation test is described for assessing relative abundance predictions from the maximum entropy approach to biodiversity. The null model underlying the test randomly allocates observed abundances to species, but retains key aspects of the structure of the observed communities; site richness, species composition, and trait covariance. Three test statistics are used to explore different characteristics of the predictions. Two are based on pairwise comparisons between observed and predicted species abundances (RMSE, RMSESqrt). The third statistic is novel and is based on community-level abundance patterns, using an index calculated from the observed and predicted community entropies (EDiff). Validation of the test to quantify type I and type II error rates showed no evidence of bias or circularity, confirming the dependencies quantified by Roxburgh and Mokany (2007) and Shipley (2007) have been fully accounted for within the null model. Application of the test to the vineyard data of Shipley et al. (2006) and to an Australian grassland dataset indicated significant departures from the null model, suggesting the integration of species trait information within the maximum entropy framework can successfully predict species abundance patterns. The paper concludes with some general comments on the use of maximum entropy in ecology, including a discussion of the mathematics underlying the Maxent optimisation algorithm and its implementation, the role of absent species in generating biased predictions, and some comments on determining the most appropriate level of data aggregation for Maxent analysis. [source] Recent advances in permafrost modellingPERMAFROST AND PERIGLACIAL PROCESSES, Issue 2 2008Daniel Riseborough Abstract This paper provides a review of permafrost modelling advances, primarily since the 2003 permafrost conference in Zürich, Switzerland, with an emphasis on spatial permafrost models, in both arctic and high mountain environments. Models are categorised according to temporal, thermal and spatial criteria, and their approach to defining the relationship between climate, site surface conditions and permafrost status. The most significant recent advances include the expanding application of permafrost thermal models within spatial models, application of transient numerical thermal models within spatial models and incorporation of permafrost directly within global circulation model (GCM) land surface schemes. Future challenges for permafrost modelling will include establishing the appropriate level of integration required for accurate simulation of permafrost-climate interaction within GCMs, the integration of environmental change such as treeline migration into permafrost response to climate change projections, and parameterising the effects of sub-grid scale variability in surface processes and properties on small-scale (large area) spatial models. Copyright © 2008 John Wiley & Sons, Ltd. [source] Minimum Reporting Requirements for Proteomics: A MIAPE PrimerPROTEINS: STRUCTURE, FUNCTION AND BIOINFORMATICS, Issue S2 2006Chris F. Taylor Dr.Article first published online: 9 OCT 200 Amongst other functions, the Human Proteome Organization's Proteomics Standards Initiative (HUPO PSI) facilitates the generation by the proteomics community of guidelines that specify the appropriate level of detail to provide when describing the various components of a proteomics experiment. These guidelines are codified as the MIAPE (Minimum Information About a Proteomics Experiment) specification, the first modules of which are now finalized. This primer describes the structure and scope of MIAPE, places it in context amongst reporting specifications for other domains, briefly discusses related informatics resources and closes by considering the ramifications for the proteomics community. [source] Aldosterone responsiveness of the epithelial sodium channel (ENaC) in colon is increased in a mouse model for Liddle's syndromeTHE JOURNAL OF PHYSIOLOGY, Issue 2 2008Marko Bertog Liddle's syndrome is an autosomal dominant form of human hypertension, caused by gain-of-function mutations of the epithelial sodium channel (ENaC) which is expressed in aldosterone target tissues including the distal colon. We used a mouse model for Liddle's syndrome to investigate ENaC-mediated Na+ transport in late distal colon by measuring the amiloride-sensitive transepithelial short circuit current (,ISC-Ami) ex vivo. In Liddle mice maintained on a standard salt diet, ,ISC-Ami was only slightly increased but plasma aldosterone (PAldo) was severely suppressed. Liddle mice responded to a low or a high salt diet by increasing or decreasing, respectively, their PAldo and ,ISC-Ami. However, less aldosterone was required in Liddle animals to achieve similar or even higher Na+ transport rates than wild-type animals. Indeed, the ability of aldosterone to stimulate ,ISC-Ami was about threefold higher in Liddle animals than in the wild-type controls. Application of aldosterone to colon tissue in vitro confirmed that ENaC stimulation by aldosterone was not only preserved but enhanced in Liddle mice. Aldosterone-induced transcriptional up-regulation of the channel's ,- and ,-subunit (,ENaC and ,ENaC) and of the serum- and glucocorticoid-inducible kinase 1 (SGK1) was similar in colon tissue from Liddle and wild-type animals, while aldosterone had no transcriptional effect on the ,-subunit (,ENaC). Moreover, Na+ feedback regulation was largely preserved in colon tissue of Liddle animals. In conclusion, we have demonstrated that in the colon of Liddle mice, ENaC-mediated Na+ transport is enhanced with an increased responsiveness to aldosterone. This may be pathophysiologically relevant in patients with Liddle's syndrome, in particular on a high salt diet, when suppression of PAldo is likely to be insufficient to reduce Na+ absorption to an appropriate level. [source] The evolution of arthropod limbsBIOLOGICAL REVIEWS, Issue 2 2004Geoff A. Boxshall ABSTRACT Limb morphology across the arthropods is reviewed using external morphological and internal anatomical data from both recent and fossil arthropods. Evolutionary trends in limb structure are identified primarily by reference to the more rigorous of the many existing phylogenetic schemes, but no major new phylogenetic inferences are presented. Tagmosis patterns are not considered, although the origins and patterns of heteronomy within the postantennulary limb series are analysed. The phenomenon of annulation is examined and two basic types of annuli are recognised: terminal and intercalary. The annulation of the apical segment of a limb results in the formation of terminal flagella, and is typical of primarily sensory appendages such as insect and malacostracan antennules and maxillary palps of some hexapods. Intercalary annulation, arising by subdivision of existing subterminal segments, is common, particularly in the tarsal region of arthropodan walking limbs. Differentiating between segments and annuli is discussed and is recognised as a limiting factor in the interpretation of fossils, which usually lack information on intrinsic musculature, and in the construction of groundplans. Rare examples of secondary segmentation, where the criteria for distinguishing between segments and annuli fail, are also highlighted. The basic crown-group arthropodan limb is identified as tripartite, comprising protopodite, telopodite and exopodite, and the basic segmentation patterns of each of these parts are hypothesised. Possible criteria are discussed that can be used for establishing the boundary between protopodite and telopodite in limbs that are uniramous through loss of the exopodite. The subdivision of the protopodite, which is typical of the postantennulary limbs of mandibulates, is examined. The difficulties resulting from the partial or complete failure of expression of articulations within the mandibulate protopodite and subsequent incorporation of partial proto-podal segments into the body wall, are also discussed. The development and homology between the various exites, including gills, on the postantennulary limbs of arthropods are considered in some detail, and the question of the possible homology between crustacean gills and insect wings is critically addressed. The hypothesis that there are only two basic limb types in arthropods, antennules and postantennulary limbs, is proposed and its apparent contradiction by the transformation of antennules into walking limbs by homeotic mutation is discussed with respect to the appropriate level of serial homology between these limbs. [source] An assessment and ranking of barriers to doing environmental business with ChinaBUSINESS STRATEGY AND THE ENVIRONMENT, Issue 6 2009Turlough F. Guerin Abstract The transfer of environmental goods and services to China will increasingly be of importance to developed nations as the demand for environmental management services increases in China. A review of the literature on technology transfer to China revealed a range of well recognized and commonly known constraints to transferring technologies to China. There were gaps in the literature in relation to the concerns that environmental professionals have regarding technology transfer to China, as there is limited information on the transfer in environmental goods and services to China. A survey of the non-trade barriers and their practical impact on the transfer of environmental technologies and goods and services to China, focusing on Australian suppliers, was undertaken to address these gaps. The survey, which was developed from barriers to technology transfer already described in the extensive research addressing the wider issues of technology transfer to China, targeted environmental professionals but also included other professionals with interests in transferring environmental goods and services to China. From the survey, the highest priority barriers to transferring environmental goods and service to China were identified, and those that are most likely to limit Australian vendors of environmental goods and services in their technology transfers to China were protection of intellectual property (IP), limitations of the rule of law, fragmentation and bureaucracy of the Chinese government and establishing appropriate level of ownership (of environmental goods and services providers in China). Examples of Australian experience were also examined, which confirmed these barriers to providing the needed technology and innovation to manage China's increasing environmental impacts. The research also shows that the barriers identified do not appear to be unique to transfer of environmental goods and services but rather generic to the transfer and adoption of Australian technology into China. Copyright © 2007 John Wiley & Sons, Ltd and ERP Environment. [source] The influence of bladder filling on anorectal functionCOLORECTAL DISEASE, Issue 3 2003J. J. Crosbie Abstract Objective The aim of this study was to develop a technique to simultaneously evaluate bladder and anorectal function. In particular, this study was designed to determine if anal sphincter resting pressure, anal sphincter squeeze pressure and rectal sensation change with bladder filling. Patient and methods A pilot study of ten female patients who presented to the pelvic physiology unit for assessment of urinary symptoms was performed. All patients completed a symptom questionnaire and quality of life assessment form. Following informed consent a baseline urodynamic test was performed with the bladder empty and subsequently followed by an anorectal manometric test. Changes in anal sphincter resting pressure, squeeze pressure and rectal pressure were recorded over a ten-minute period. With the patient lying in the left lateral position, the bladder was then filled with isotonic saline at room temperature at a constant rate of 30ml/min. A continuous assessment of changes in anal sphincter resting pressure during bladder filling was made. Anal sphincter squeeze pressure and rectal sensation were measured at fixed intervals during bladder filling (50, 100, 150 ml etc.) and at fixed intervals relative to bladder capacity (25, 50, 75 and 100% capacity) by stopping bladder filling at the appropriate level. Results , There was no significant change in anal sphincter resting pressure (Mean difference(s.d.) between bladder full and empty = 2.7(5.6) P = 0.92*), squeeze pressure (Mean(s.d.) difference = 9.5(26.3) P = 0.86*) and rectal sensation (Mean difference(s.d.) first sensation 10(15.2) P = 0.958; Mean difference(s.d.) urgency = 10(17.8) P = 0.07*) on bladder filling. Conclusion , Under normal physiological circumstances, bladder filling does not influence anorectal function. *Stastistical analysis: Wilcoxon signed rank sum test. P < 0.05 considered significant. Units = mmHg [source] Activity-based restorative therapies: Concepts and applications in spinal cord injury-related neurorehabilitationDEVELOPMENTAL DISABILITIES RESEARCH REVIEW, Issue 2 2009Cristina L. Sadowsky Abstract Physical rehabilitation following spinal cord injury-related paralysis has traditionally focused on teaching compensatory techniques, thus enabling the individual to achieve day-to-day function despite significant neurological deficits. But the concept of an irreparable central nervous system (CNS) is slowly being replaced with evidence related to CNS plasticity, repair, and regeneration, all related to persistently maintaining appropriate levels of neurological activity both below and above the area where the damage occurred. It is now possible to envision functional repair of the nervous system by implementing rehabilitative interventions. Making the transition from "bench to bedside" requires careful analysis of existing basic science evidence, strategic focus of clinical research, and pragmatic implementation of new therapeutic tools. Activity, defined as both function specific motor task and exercise appears to be a necessity for optimization of functional, metabolic, and neurological status in chronic paralysis. Crafting a comprehensive rehabilitative intervention focused on functional improvement through neurological gains seems logical. The terms activity-based restorative therapies, activity-based therapies, and activity-based rehabilitation have been coined in the last 10 years to describe a new fundamental approach to deficits induced by neurological paralysis. The goal of this approach is to achieve activation of the neurological levels located both above and below the injury level using rehabilitation therapies. This article reviews basic and clinical science evidence pertaining to implementation of physical activity and exercise as a therapeutic tool in the management of chronic spinal cord-related neurological paralysis. © 2009 Wiley-Liss, Inc. Dev Disabil Res Rev 2009;15:112,116. [source] Kinematic response functions and dynamic stiffnesses of bridge embankmentsEARTHQUAKE ENGINEERING AND STRUCTURAL DYNAMICS, Issue 11 2002Jian Zhang Abstract Recognizing that soil,structure interaction affects appreciably the earthquake response of highway overcrossings, this paper compares approximate analytical solutions and finite element results to conclude on a simple procedure that allows for the estimation of the kinematic response functions and dynamic stiffnesses of approach embankments. It is shown that the shear-wedge model yields realistic estimates for the amplification functions of typical embankments and reveals the appropriate levels of dynamic strains which are subsequently used to estimate the stiffness and damping coefficients of embankments. The shear-wedge model is extended to a two-dimensional model in order to calculate the transverse static stiffness of an approach embankment loaded at one end. The formulation leads to a sound closed-form expression for the critical length, Lc, that is the ratio of the transverse static stiffness of an approach embankment and the transverse static stiffness of a unit-width wedge. It is shown through two case studies that the transverse dynamic stiffness (,spring' and ,dashpot') of the approach embankment can be estimated with confidence by multiplying the dynamic stiffness of the unit-width wedge with the critical length, Lc. The paper concludes that the values obtained for the transverse kinematic response function and dynamic stiffness can also be used with confidence to represent the longitudinal kinematic response function and dynamic stiffness, respectively. Copyright © 2002 John Wiley & Sons, Ltd. [source] Implementation of chemometric methodology in ACE: Predictive investigation of protein,ligand bindingELECTROPHORESIS, Issue 16 2007Grady Hanrahan Abstract An ACE predictive investigation of protein,ligand binding using a highly effective chemometric response surface design technique is presented. Here, Kd was estimated using one noninteracting standard which relates to changes in the electrophoretic mobility of carbonic anhydrase B (CAB, EC 4.2.1.1) on complexation with the ligand 4-carboxybenzenesulfonamide (CBSA) present in the electrophoresis buffer. Experimental factors including injection time, capillary length, and applied voltage were selected and tested at three levels in a Box,Behnken design. Statistical analysis results were used to create a mathematical model for response surface prediction via contour and surface plots at a given target response (Kd,=,1.19×10,6,M). As expected, there were a number of predicted solutions that reached our target response based on the significance of each factor at appropriate levels. The adequacy of the model was validated by experimental runs with the predicted model solution (capillary length,=,47,cm, voltage,=,11,kV, injection time,=,0.01,min) presented in detail as an example. [source] The extrapolation problem and how population modeling can help,ENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 10 2008Valery E. Forbes Abstract We argue that population modeling can add value to ecological risk assessment by reducing uncertainty when extrapolating from ecotoxicological observations to relevant ecological effects. We review other methods of extrapolation, ranging from application factors to species sensitivity distributions to suborganismal (biomarker and "-omics") responses to quantitative structure,activity relationships and model ecosystems, drawing attention to the limitations of each. We suggest a simple classification of population models and critically examine each model in an extrapolation context. We conclude that population models have the potential for adding value to ecological risk assessment by incorporating better understanding of the links between individual responses and population size and structure and by incorporating greater levels of ecological complexity. A number of issues, however, need to be addressed before such models are likely to become more widely used. In a science context, these involve challenges in parameterization, questions about appropriate levels of complexity, issues concerning how specific or general the models need to be, and the extent to which interactions through competition and trophic relationships can be easily incorporated. [source] EFNS guideline on diagnosis and management of limb girdle muscular dystrophiesEUROPEAN JOURNAL OF NEUROLOGY, Issue 12 2007F. Norwood The limb girdle muscular dystrophies (LGMD) are termed as such as they share the characteristic feature of muscle weakness predominantly affecting the shoulder and pelvic girdles; their classification has been completely revised in recent years because of elucidation of many of the underlying genetic and protein alterations in the various subtypes. An array of diagnostic measures is possible but with varying ease of use and availability. Several aspects of muscle cell function appear to be involved in the causation of muscle pathology. These cellular variations may confer some specific clinical features thus permitting recognition of the LGMD subtype and hence directing appropriate levels of monitoring and intervention. Despite an extensive literature on the individual limb girdle dystrophies, these publications may be impenetrable for the general neurologist in this increasingly complex field. The proposed guidelines suggest an approach to the diagnosis and monitoring of the limb girdle dystrophies in a manner accessible to general neurologists. [source] How to use laparoscopic surgical instruments safelyINTERNATIONAL JOURNAL OF UROLOGY, Issue 3 2009Eiji Higashihara The development of laparoscopic surgery has been accompanied by a rapid increase in the number of laparoscopic surgical procedures carried out in the field of urology. In 2002 laparoscopic nephrectomy was approved for coverage under Japanese national health insurance, and in 2003 there were over 1000 registered cases in which this procedure was carried out. This suggests that laparoscopic nephrectomy, a procedure formerly conducted at only a few institutions, is now spreading to hospitals across Japan. Laparoscopic surgery involves the use of specialized instruments within a restricted field of vision, and risky surgical techniques can potentially result in visceral or vascular damage. In order to promote the use of safe laparoscopic surgery procedures, the Japanese Urological Association and the Japanese Society of Endourology and Extracorporeal Shock Wave Lithotripsy (ESWL) have inaugurated a certification program for urologic laparoscopy. This program not only encourages development in this field of surgery and provides technical certification to ensure appropriate levels of expertise, but also reviews methods for the correct use of instruments such as trocars and hemostats. The purpose of this video is to present correct methods for the use of a variety of laparoscopic instruments, in order to increase the safety of this procedure. The video has been designed to be useful not only for practitioners who are just beginning laparoscopy, but also for those who already have extensive laparoscopic experience. The video discusses five laparoscopic instruments (trocar, electric surgical devices, ultrasonic surgery devices, clips and clip appliers and endo-staplers), and demonstrates their correct use. In addition, animal models are used to illustrate the potential complications that can be associated with some methods of use. [source] Fibrodysplasia Ossificans Progressiva (FOP), a Disorder of Ectopic Osteogenesis, Misregulates Cell Surface Expression and Trafficking of BMPRIA,,JOURNAL OF BONE AND MINERAL RESEARCH, Issue 7 2005Lourdes Serrano de la Peña Abstract FOP is a disorder in which skeletal muscle is progressively replaced with bone. FOP lymphocytes, a model system for exploring the BMP pathway in these patients, exhibit a defect in BMPRIA internalization and increased activation of downstream signaling, suggesting that altered BMP receptor trafficking underlies ectopic bone formation in this disease. Introduction: Fibrodysplasia ossificans progressiva (FOP) is a severely disabling disorder characterized by progressive heterotopic ossification of connective tissues. Whereas the genetic defect and pathophysiology of this condition remain enigmatic, BMP4 mRNA and protein are overexpressed, and mRNAs for a subset of secreted BMP antagonists are not synthesized at appropriate levels in cultured lymphocytes from FOP patients. These data suggest involvement of altered BMP signaling in the disease. In this study, we investigate whether the abnormality is associated with defective BMP receptor function in lymphocytes. Materials and Methods: Cell surface proteins were quantified by fluorescence-activated cell sorting (FACS). Protein phosphorylation was assayed by immunoprecipitation and immunoblotting. Protein synthesis and degradation were examined by [35S]methionine labeling and pulse-chase assays. mRNA was detected by RT-PCR. Results: FOP lymphocytes expressed 6-fold higher levels of BMP receptor type IA (BMPRIA) on the cell surface compared with control cells and displayed a marked reduction in ligand-stimulated internalization and degradation of BMPRIA. Moreover, in control cells, BMP4 treatment increased BMPRIA phosphorylation, whereas BMPRIA showed ligand-insensitive constitutive phosphorylation in FOP cells. Our data additionally support that the p38 mitogen-activated protein kinase (MAPK) signaling pathway is a major BMP signaling pathway in these cell lines and that expression of inhibitor of DNA binding and differentiation 1 (ID-1), a transcriptional target of BMP signaling, is enhanced in FOP cells. Conclusions: These data extend our previous observations of misregulated BMP4 signaling in FOP lymphocytes and show that cell surface overabundance and constitutive phosphorylation of BMPRIA are associated with a defect in receptor internalization. Altered BMP receptor trafficking may play a significant role in FOP pathogenesis. [source] Targeting nitric oxide for cancer therapyJOURNAL OF PHARMACY AND PHARMACOLOGY: AN INTERNATI ONAL JOURNAL OF PHARMACEUTICAL SCIENCE, Issue 1 2007David Hirst A blueprint for the ideal anticancer molecule would include most of the properties of nitric oxide (NO,), but the ability to exploit these characteristics in a therapeutic setting requires a detailed understanding of the biology and biochemistry of the molecule. These properties include the ability of NO, to affect tumour angiogenesis, metastasis, blood flow and immuno surveillance. Furthermore NO, also has the potential to enhance both radio- and chemotherapy. However, all of these strategies are dependent on achieving appropriate levels of NO,, since endogenous levels of NO, appear to have a clear role in tumour progression. This review aims to summarize the role of NO, in cancer with particular emphasis on how the properties of NO, can be exploited for therapy. [source] Devolution in Uganda: an experiment in local service deliveryPUBLIC ADMINISTRATION & DEVELOPMENT, Issue 3 2003Robin Mitchinson The ongoing programme for reform and reduction of the public service in Uganda relies heavily on the devolution of provision and delivery of most major public services to the lowest appropriate levels (primarily the District Councils,), and therefore local government is becoming a key element in the search for new ways of governance. The rationale is that the overriding problem, as in much of Africa, is poverty and that the most effective way of tackling it is by the empowerment of the people to provide the services that they judge necessary and to decide their own local priorities in the allocation of resources. Whether the experiment succeeds will be determined in large measure by the ability and desire of the Government to ensure that local authorities have access to at least the same levels of resources as the previous service providers. Of equal importance is the capacity and ability of local government to meet the challenge, and this begs the question as to whether professional staff have the experience and competence and whether the elected members have the political skills, probity and integrity for the task. The policies have been well thought-out and the solutions appear to be capable of implementation, but the ,people factor' will also be critical to success. The present scenario is guardedly encouraging; the devolution programme will probably meet sufficient of its objectives to justify the changes, given continuing donor support, and performance will improve as local authorities gain experience and self-confidence. For its part, Government will need to resist the temptation to over-supervise, and intervene only sparingly. Copyright © 2003 John Wiley & Sons, Ltd. [source] | ||||||||||||||||||||||||||||