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Mixed Mode (mixed + mode)
Selected AbstractsAchiral and chiral separations using MEKC, polyelectrolyte multilayer coatings, and mixed mode separation techniques with molecular micellesELECTROPHORESIS, Issue 6 2010Candace A. Luces Abstract Mixed mode (MM) separation using a combination of MEKC and polyelectrolyte multilayer (PEM) coatings is herein reported for the separation of achiral and chiral analytes. Many analytes are difficult to separate by MEKC and PEM coatings alone. Therefore, the implementation of a MM separation provides several advantages for overcoming the limitations of these well-established methods. In this study, it was observed that achiral separations using MEKC and PEM coatings individually resulted in partial resolution of eight very similar aryl ketones when the molecular micelle (sodium poly(N -undecanoyl- L -glycinate)) concentration was varied from 0.25 to 1.00%,w/v and the bilayer number varied from 2 to 4. However, when MM separation was introduced, baseline resolution was achieved for all eight analytes. In the case of chiral separations, temazepam, aminoglutethimide, benzoin, benzoin methyl ether, and coumachlor were separated using the three separation techniques. For chiral separations, the chiral molecular micelle, sodium poly(N -undecanoyl- L -leucylvalinate), was employed at concentrations of 0.25,1.50%,w/v for both MEKC and PEM coatings. Overall, the results revealed partial separation with MEKC and PEM coatings individually. However, MM separation enabled baseline separation of each chiral mixture. The separation of achiral and chiral compounds from different compound classes demonstrates the versatility of this MM approach. [source] Fatigue crack growth under variable amplitude loading Part I: experimental investigationsFATIGUE & FRACTURE OF ENGINEERING MATERIALS AND STRUCTURES, Issue 4 2006M. SANDER ABSTRACT During use, a component or a structure is exposed to variable amplitude loading, which influences the lifetime. Within the scope of this work, systematic investigations of different loading situations are carried out by means of experimental studies (part I) as well as analytical and numerical studies (part II). The experimental investigations show that overloads lead to retardation effects, which are influenced by several factors, e.g. the overload ratio, baseline-level loading, number of overloads or the fraction of mixed mode. In a high,low,high block loading, both retarded and accelerated crack growth can be obtained, which is also influenced, e.g. by the block loading ratio and the length of the block. Moreover, experimental studies have been performed with load spectra, like FELIX/28, CARLOS vertical and WISPER. They have been applied in original form as well as in counted and reconstructed sequences. [source] Theoretical crack path predictionFATIGUE & FRACTURE OF ENGINEERING MATERIALS AND STRUCTURES, Issue 1-2 2005H. A. RICHARD ABSTRACT In many practical cases, the crack growth leads to abrupt failure of components and structures. For reasons of a reliable quantification of the endangerment due to sudden fracture of a component, therefore, it is of enormous importance to know the threshold values, the crack paths and the growth rates for the fatigue crack growth as well as the limiting values for the beginning of unstable crack growth (fracture toughness). This contribution deals with the complex problem of a,however initiated,crack, that is subjected to a mixed-mode loading. It will present the hypotheses and concepts, which describe the superposition of Mode I and Mode II (plane mixed mode) as well as the superposition of all three modes (Mode I, II and III) for spatial loading conditions. Those concepts admit a quantitative appraisal of such crack situations and a characterization of possible crack paths. [source] The effect of thermocycling on peel strength of six soft lining materialsJOURNAL OF ORAL REHABILITATION, Issue 6 2002A. Sertgöz The bond strength of two heat-cured and four cold-cured soft lining materials was compared using a peeling test method before and after thermocycling. Tested soft lining materials were Molloplast B, Mollosil, Ufigel P, Ufigel C, Permaquick and Permaflex. Six specimens, 6·5 × 2 × 0·3 cm, for each group were prepared according to the manufacturers' instructions. Control groups were stored in a humidor for 24 h, whereas the others were thermocycled in a water bath between the 5 and 55 °C for 5000 cycles. Peel strength of samples were measured using an Instron Universal testing machine at a cross-head speed of 5 mm min,1. The types of failure were observed using an electron microscope. The highest peel bond strength values were calculated for Permaflex and Permaquick before and after thermocycling, respectively. Molloplast B, Mollosil, Ufigel P and Permaquick demonstrated an increase in peel strength after thermocycling, with Permaquick lining material having statistically significant increase. However, decrease in peel strength was observed for Ufigel C and Permaflex after thermocycling. Failure mode within the control groups was cohesive for Molloplast B, Permaquick and Permaflex, whereas adhesive for Ufigel P and Ufigel C. Mollosil demonstrated a mixed mode of failure for both thermocycled and control groups. [source] Effect of Microwave Disinfection Procedures on Torsional Bond Strengths of Two Hard Chairside Denture Reline MaterialsJOURNAL OF PROSTHODONTICS, Issue 6 2006Ana Lucia Machado DDS Purpose: This study evaluated the potential effects of denture base resin water storage time and an effective denture disinfection method (microwave irradiation at 650 W for 6 minutes) on the torsional bond strength between two hard chairside reline resins (GC Reline and New Truliner) and one heat-polymerizing denture base acrylic resin (Lucitone 199). Materials and Methods: Cylindrical (30 × 3.9 mm) denture base specimens (n= 160) were stored in water at 37°C (2 or 30 days) before bonding. A section (3.0 mm) was removed from the center of the specimens, surfaces prepared, and the reline materials packed into the space. After polymerization, specimens were divided into four groups (n= 10): Group 1 (G1),tests performed after bonding; Group 2 (G2),specimens immersed in water (200 ml) and irradiated twice (650 W for 6 minutes); Group 3 (G3),specimens irradiated daily until seven cycles of disinfection; Group 4 (G4),specimens immersed in water (37°C) for 7 days. Specimens were submitted to a torsional test (0.1 Nm/min), and the torsional strengths (MPa) and the mode of failure were recorded. Data from each reline material were analyzed by a two-way analysis of variance, followed by Neuman-Keuls test (p= 0.05). Results: For both Lucitone 199 water storage periods, before bonding to GC Reline resin, the mean torsional strengths of G2 (2 days,138 MPa; 30 days,132 MPa), G3 (2 days,126 MPa; 30 days,130 MPa), and G4 (2 days,130 MPa; 30 days,137 MPa) were significantly higher (p < 0.05) than G1 (2 days,108 MPa; 30 days,115 MPa). Similar results were found for Lucitone 199 specimens bonded to New Truliner resin, with G1 specimens (2 days,73 MPa; 30 days,71 MPa) exhibiting significantly lower mean torsional bond strength (p < 0.05) than G2 (2 day,86 MPa; 30 days,90 MPa), G3 (2 days,82 MPa; 30 days,82 MPa), and G4 specimens (2 days,78 MPa; 30 days,79 MPa). The adhesion of both materials was not affected by water storage time of Lucitone 199 (p > 0.05). GC reline showed a mixed mode of failure (adhesive/cohesive) and New Truliner failed adhesively. Conclusions: Up to seven microwave disinfection cycles did not decrease the torsional bond strengths between the hard reline resins, GC Reline and New Truliner to the denture base resin Lucitone 199. The effect of additional disinfection cycles on reline material may be clinically significant and requires further study. [source] Separation and determination of five major opium alkaloids with mixed mode of hydrophilic/cation-exchange monolith by pressurized capillary electrochromatographyJOURNAL OF SEPARATION SCIENCE, JSS, Issue 17 2007Xucong Lin Abstract A method for the separation and determination of five major opium alkaloids (narcotine, papaverine, thebaine, codeine, and morphine) in pericarpium papaveris by pressurized CEC (pCEC) with monolithic column has been developed. Under the optimum condition, linear calibration ranges of narcotine, papaverine, thebaine, codeine, and morphine were obtained as 2,85, 2,85, 5,75, 10,65, and 10,65 ,g/mL, respectively. LODs of these analytes were 1.5,6.0 ,g/mL. The RSD (n = 7) of the migration time and peak area were 1.94,5.24 and 4.05,8.21%, respectively. The proposed method was successfully applied to the analysis of pericarpium papaveris samples. Average recoveries of 79.0,95.9% at different fortified levels of alkaloids were achieved with RSD less than 4.6%. Meanwhile, the mechanism of the separation of the alkaloids on the monolithic column was also discussed. The result showed that the separation of alkaloids was mainly based on the mixed mode of hydrophilic interaction (HI) and cation exchange. [source] Salinity-related desertification and management strategies: Indian experienceLAND DEGRADATION AND DEVELOPMENT, Issue 4 2009G. Singh Abstract High concentration of salts in the rootzone soil limits the productivity of nearly 953 million ha of productive land in the world. Australia, followed by Asia, has the largest area under salinity and sodicity. Most of the salt-affected soils and brackish ground water resources are confined to arid and semiarid regions and are the causative factors for triggering the process of desertification. The problem of salinity and sodicity has degraded about 6·73 million ha area in India. Secondary salinization associated with introduction of irrigation in dry areas like Thar desert in the western part of the country and Sharda Sahayak in Central India have caused desertification due to rise of salts with the rise in ground water level. Large scale cultivation of prawns using sea water in coastal Andhra Pradesh and elsewhere rendered about 2.1 million ha area unfit for agriculture. Similarly, 30,84 per cent ground water in north-western states of the country is either saline and /or brackish and is unfit for irrigation. Use of marginal quality water for irrigation has rendered several thousand ha of productive land unfit for cultivation. The Central Soil Salinity Research Institute was established in 1969 at Karnal to develop sustainable and eco-friendly technologies for reclamation and management of salt-affected soils and judicious use of marginal quality waters. The institute has developed location-specific techniques for reversion of salinity related desertification in India. Salient findings of research during the last three decades and more are presented in this review. This paper deals with (a) classification, nature and extent of salt-affected soils and poor quality water in India, (b) case studies/socio-economic concerns of salinity related desertification, (c) chemical, hydrological and biological approaches in use for rehabilitation of salt-affected soils, (d) guidelines for safe and productive use of marginal quality ground water through cyclic and mixed mode and precision irrigation techniques, (e) successful rehabilitation case studies, (f) alternate land use practices such as raising forest plantations, horticulture, agroforestry, high value medicinal, aromatic and flowering crops, etc., (g) technological, social, economic and environmental impacts and (h) future line of research. Issues requiring policy initiatives to halt salinity-related desertification are also discussed in this review paper. Copyright © 2009 John Wiley & Sons, Ltd. [source] Development and characterization of an all-olefin thermoplastic sandwich composite systemPOLYMER COMPOSITES, Issue 4 2002Ganesan Kumar In this investigation an all-olefin thermoplastic sandwich system was developed and characterized. Commingled glass fiber polypropylene (PP) composite was used as skin and HDPE (PE) foam with closed cells as core. Infra-red heating was used for melting the surfaces of the substrates for surface fusion bonding with a cold press. Two tie layer films, viz. ethylene-propylene copolymer (EPC) and HDPE/elastomer blend, were used as hot melt adhesives for bonding the substrates. Single lap shear joints were prepared from PP composite and PE foam adherends with a bonding area of 25.4 mm × 25.4 mm to determine the interface strength. EPC tie layer provided higher bond strength (27.4 kg/cm2) to the all-olefin sandwich system than HDPE/elastomer blend based one (19.7 kg/cm2). For EPC tie layer based sandwiches, a mixed mode a failure was observed in the failed lap shear samples; about 40% is cohesive failure through tie layer, and the rest of failure was adhesive either at PP composite or PE surfaces. Environmental scanning electron micrographs (ESEM) reveal that in the process of surface fusion bonding, PE foam cells in the vicinity of 0.80 mm interphase area were coalesced with high temperature and pressure. No macro level penetration of tie layer melt front into foam cells was observed. As the surface morphology of foam was altered on account of IR surface heating and the PP composite bonding side had a resin-rich layer, the bonding situation was closer to that between two polymer film surface. [source] High-efficiency peptide analysis on monolithic multimode capillary columns: Pressure-assisted capillary electrochromatography/capillary electrophoresis coupled to UV and electrospray ionization-mass spectrometryELECTROPHORESIS, Issue 21 2003Alexander R. Ivanov Abstract High-efficiency peptide analysis using multimode pressure-assisted capillary electrochromatography/capillary electrophoresis (pCEC/pCE) monolithic polymeric columns and the separation of model peptide mixtures and protein digests by isocratic and gradient elution under an applied electric field with UV and electrospray ionization-mass spectrometry (ESI-MS) detection is demonstrated. Capillary multipurpose columns were prepared in silanized fused-silica capillaries of 50, 75, and 100 ,m inner diameters by thermally induced in situ copolymerization of methacrylic monomers in the presence of n -propanol and formamide as porogens and azobisisobutyronitrile as initiator. N -Ethylbutylamine was used to modify the chromatographic surface of the monolith from neutral to cationic. Monolithic columns were termed as multipurpose or multimode columns because they showed mixed modes of separation mechanisms under different conditions. Anion-exchange separation ability in the liquid chromatography (LC) mode can be determined by the cationic chromatographic surface of the monolith. At acidic pH and high voltage across the column, the monolithic stationary phase provided conditions for predominantly capillary electrophoretic migration of peptides. At basic pH and electric field across the column, enhanced chromatographic retention of peptides on monolithic capillary column made CEC mechanisms of migration responsible for separation. The role of pressure, ionic strength, pH, and organic content of the mobile phase on chromatographic performance was investigated. High efficiencies (exceeding 300,000 plates/m) of the monolithic columns for peptide separations are shown using volatile and nonvolatile, acidic and basic buffers. Good reproducibility and robustness of isocratic and gradient elution pressure-assisted CEC/CE separations were achieved for both UV and ESI-MS detection. Manipulation of the electric field and gradient conditions allowed high-throughput analysis of complex peptide mixtures. A simple design of sheathless electrospray emitter provided effective and robust low dead volume interfacing of monolithic multimode columns with ESI-MS. Gradient elution pressure-assisted mixed-mode separation CE/CEC-ESI-MS mass fingerprinting and data-dependent pCE/pCEC-ESI-MS/MS analysis of a bovine serum albumin (BSA) tryptic digest in less than 5 min yielding high sequence coverage (73%) demonstrated the potential of the method. [source] HOST GROWTH CONDITIONS INFLUENCE EXPERIMENTAL EVOLUTION OF LIFE HISTORY AND VIRULENCE OF A PARASITE WITH VERTICAL AND HORIZONTAL TRANSMISSIONEVOLUTION, Issue 7 2010Hélène Magalon In parasites with mixed modes of transmission, ecological conditions may determine the relative importance of vertical and horizontal transmission for parasite fitness. This may lead to differential selection pressure on the efficiency of the two modes of transmission and on parasite virulence. In populations with high birth rates, increased opportunities for vertical transmission may select for higher vertical transmissibility and possibly lower virulence. We tested this idea in experimental populations of the protozoan Paramecium caudatum and its bacterial parasite Holospora undulata. Serial dilution produced constant host population growth and frequent vertical transmission. Consistent with predictions, evolved parasites from this "high-growth" treatment had higher fidelity of vertical transmission and lower virulence than parasites from host populations constantly kept near their carrying capacity ("low-growth treatment"). High-growth parasites also produced fewer, but more infectious horizontal transmission stages, suggesting the compensation of trade-offs between vertical and horizontal transmission components in this treatment. These results illustrate how environmentally driven changes in host demography can promote evolutionary divergence of parasite life history and transmission strategies. [source] | ||||||||||||||||