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Medicinal Preparations (medicinal + preparation)
Selected AbstractsTwo polymorphs of morpholin-4-ium 2-(5-methyl-1H -1,2,4-triazol-3-ylsulfanyl)acetateACTA CRYSTALLOGRAPHICA SECTION C, Issue 1 2009Svetlana V. Shishkina Two polymorphs of the title organic salt (a very effective medicinal preparation with the commercial name thiotriazoline), C4H10NO+·C5H6N3O2S,, were obtained. The cations and anions are connected by hydrogen bonds and extend into two-dimensional networks. The main packing motifs are an R44(12) cluster in the monoclinic form and a chain in the orthorhombic form. [source] Simultaneous determination of albi,orin and paeoni,orin in rat urine by solid-phase extraction and high-performance liquid chromatography following oral administration of Si,Wu decoctionBIOMEDICAL CHROMATOGRAPHY, Issue 10 2004Yuxin Sheng Abstract A high performance liquid chromatographic method (HPLC), together with solid phase extraction (SPE), was developed for simultaneous determination of albi,orin and paeoni,orin in rat urine after oral administration of Si,Wu decoction. The samples were pretreated with solid phase extraction using Extract-CleanÔ cartridges. Analysis of the extract was performed on a reversed-phase C18 column and a mobile phase made up of acetonitrile and 0.03% formic acid (17:83, v/v). UV detection was set at 230 nm. The assay was linear over the range 2.625,52.50 mg/mL for albi,orin and 3.875,77.50 µg/mL for paeoni,orin. The average percentage recoveries of three spiked urines were 97.01 ± 3.32 and 102.32 ± 6.97 for albi,orin and paeoni,orin, respectively. The intra-day precision (RSD) ranged from 0.21 to 1.79% at concentrations of 4.20, 10.50, 26.25 and 39.375 µg/mL of albi,orin and 0.12 to 2.92% at concentrations of 3.875, 10.85, 23.25 and 58.125 µg/mL of paeoni,orin, and inter-day precision (RSD) was from 1.02 to 1.86% for albi,orin and 0.94 to 3.30% for paeoni,orin, at the same four concentrations. This method was applied in order to analyze albi,orin and paeoni,orin in rat urine following oral administration of traditional Chinese medicinal preparation of Si,Wu decoction. Copyright © 2004 John Wiley & Sons, Ltd. [source] Analysis of protoberberine alkaloids in several herbal drugs and related medicinal preparations by non-aqueous capillary electrophoresisJOURNAL OF SEPARATION SCIENCE, JSS, Issue 1 2005Wen Hua Gao Abstract A simple, rapid, reproducible, and universal non-aqueous capillary electrophoresis method has been developed for the separation and determination of three major active protoberberine alkaloids including berberine, palmatine, and jatrorrhizine within 7 min. The effects of the concentrations of acetic acid and electrolyte, the ratio of organic solvent, and the applied voltage on the separation were investigated. The optimum running buffer was composed of 50 mM ammonium acetate, 0.5% (v/v) acetic acid, and 10% (v/v) acetonitrile in methanol. The applied voltage was 18 kV. The analytes were detected by UV at 214 nm. The linearities between peak areas and the concentrations of the analytes were also investigated, and they exhibit excellent linear behavior over the concentration ranges (correlation coefficients: 0.9975,0.9986). The method was successfully applied to determine the three alkaloids in several families of herbal drugs (Rhizoma Coptidis, Cortex Berberidis, Cortex Phellodendri, Herba Chelidonii,Caulis Mahoniae) and their relevant medicinal preparations for the first time, and the recoveries of the three constituents ranged between 95.6,103.2% for berberine, 97.5,103.3% for palmatine, and 96.1,103.6% for jatrorrhizine. [source] Separation and determination of active components in Radix Salviae miltiorrhizae and its medicinal preparations by nonaqueous capillary electrophoresisJOURNAL OF SEPARATION SCIENCE, JSS, Issue 7-8 2004An Jia Chen Abstract A nonaqueous capillary electrophoresis (NACE) method was developed for simultaneous assay of three bioactive components (1: cryptotanshinone; 2: tanshinone IIA, and 3: tanshinone I) in Radix Salviae miltiorrhizae and in its herbal preparations for the first time. After optimization of separation conditions, a buffer of 250 mmol L,1 ammonium acetate containing 30% acetonitrile and 1.0% acetic acid (V : V) in methanol was selected for separating the three analytes, but baseline separation of tanshinon I and tanshinone IIA was not obtained. Therefore second-order derivative electropherograms were applied for resolving overlapping peaks. Regression equations revealed good linear relationships (correlation coefficients 0.9943,0.9991) between peak heights in second-order derivative electropherograms and concentrations of the three analytes. The relative standard deviations (RSD) of the migration times and the peak height of the three constituents were in the range of 0.81,0.88% and 0.34,1.13% (intra-day), 1.57,1.86% and 3.05,5.52% (inter-day), respectively. The recoveries of three constituents ranged from 90.2 to 108.5%. The results indicated that baseline separation of the analytes was sometimes hard to obtain and second-order derivative electropherograms were applicable for the resolving and analysis of overlapping peaks. [source] Continuous capillary electrophoresis with ,ow injection and its application for determination of Ephedrine and Pseudo-ephedrine in Chinese medicinal preparationsBIOMEDICAL CHROMATOGRAPHY, Issue 8 2004Zhongwei Pan Abstract This article presents a new, simple and rapid continuous separation method by combination of ,ow injection with capillary electrophoresis designed for the analysis of basic traditional Chinese medicines. The device was produced using commercial capillary and components readily available in analytical laboratory. In double-T con,guration, the designed horizontal separation channel was 25 µm i.d. × 146 mm length (an effective separation length of 93 mm) quartz capillary, with two vertical elicitation arms produced from 0.5 mm i.d. pump tubing. The capillary was embedded in a 40 × 20 × 3 mm organic glass base. Using the double-T con,guration, continuous introduction of a series of samples was achieved. More than 3.00 resolution for ephedrine and pseudo-ephedrine were obtained using 100 mm borate buffer (pH 9.80) within 8 min in 25 µm separation channel with an electrical ,eld strength of 137 V/cm (UV detection at 215 nm). The linear calibration range was 50,1500 µg/mL (ephedrine, r = 0.9982; pseudo-ephedrine, r = 0.9990) for both analytes. The limits of detection were 2.65 µg/mL for ephedrine and 2.92 µg/mL for pseudo-ephedrine. In this device, the contents of ephedrine and pseudo-ephedrine in ,ve Chinese medicinal preparations were determined with RSDs (n = 5) in range 1.16,4.51% and recoveries in range 90.4,114.6%. Copyright © 2004 John Wiley & Sons, Ltd. [source] Determination of rhein, baicalin and berberine in traditional Chinese medicinal preparations by capillary electrophoresis with two-marker techniqueBIOMEDICAL CHROMATOGRAPHY, Issue 5 2004Liu Huitao Abstract A CZE method for the identi,cation and determination of three bioactive components, rhein, baicalin and berberine, was developed, with 10 mm borate at pH 9.20 as background electrolyte and direct UV detection at 254 nm. The two-marker (glycyrrhizin acid and cefalexin) technique was used to improve the repeatability of analysis. When the migration indices were used, the repeatability was greatly enhanced compared with migration times. The apparent dissociation constents of rhein and baicalin were also obtained. This method has been successfully applied to the simultaneous analysis of the three components with the recoveries from 96.7% to 104.6%. Copyright © 2004 John Wiley & Sons, Ltd. [source] | ||||||||||