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Medium Flow (medium + flow)
Selected AbstractsA general model for porous medium flow in squeezing film situationsLUBRICATION SCIENCE, Issue 2 2010Mohamed Nabhani Abstract The present paper deals with a numerical investigation of the hydrodynamic lubrication of a porous squeeze film between two circular discs. To this purpose, the thin film (reduced) Navier Stokes equations and a generalised porous medium model are solved. The numerical results show that the effect of the porous disc is to reduce the lubricating properties of the fluid film. This effect is increased during the squeezing action. In addition, it is shown that the film pressure, the load-carrying capacity and the velocity field based only on the Darcy model are predicted higher than those obtained from the generalised porous medium model. Copyright © 2009 John Wiley & Sons, Ltd. [source] Development of a small-scale bioreactor: Application to in vivo NMR measurementBIOTECHNOLOGY & BIOENGINEERING, Issue 2 2005Dorra Gmati Abstract A perfused bioreactor allowing in vivo NMR measurement was developed and validated for Eschscholtzia californica cells. The bioreactor was made of a 10-mm NMR tube. NMR measurement of the signal-to-noise ratio was optimized using a sedimented compact bed of cells that were retained in the bioreactor by a supporting filter. Liquid medium flow through the cell bed was characterized from a mass balance on oxygen and a dispersive hydrodynamic model. Cell bed oxygen demand for 4 h perfusion required a minimal medium flow rate of 0.8 mL/min. Residence time distribution assays at 0.8,2.6 mL/min suggest that the cells are subjected to a uniform nutrient environment along the cell bed. Cell integrity was maintained for all culture conditions since the release of intracellular esterases was not significant even after 4 h of perfusion. In vivo NMR was performed for 31P NMR and the spectrum can be recorded after only 10 min of spectral accumulation (500 scans) with peaks identified as G-6P, F-6P, cytoplasmic Pi, vacuolar Pi, ATP, and ADP,, ATP, and ADP,, NADP and NDPG, NDPG and ATP,. Cell viability was shown to be maintained as 31P chemical shifts were constant with time for all the identified nuclei, thus suggesting constant intracellular pH. © 2004 Wiley Periodicals, Inc. [source] Antibody-immobilized column for quick cell separation based on cell rollingBIOTECHNOLOGY PROGRESS, Issue 2 2010Atsushi Mahara Abstract Cell separation using methodological standards that ensure high purity is a very important step in cell transplantation for regenerative medicine and for stem cell research. A separation protocol using magnetic beads has been widely used for cell separation to isolate negative and positive cells. However, not only the surface marker pattern, e.g., negative or positive, but also the density of a cell depends on its developmental stage and differentiation ability. Rapid and label-free separation procedures based on surface marker density are the focus of our interest. In this study, we have successfully developed an antiCD34 antibody-immobilized cell-rolling column, that can separate cells depending on the CD34 density of the cell surfaces. Various conditions for the cell-rolling column were optimized including graft copolymerization, and adjustment of the column tilt angle, and medium flow rate. Using CD34-positive and -negative cell lines, the cell separation potential of the column was established. We observed a difference in the rolling velocities between CD34-positive and CD34-negative cells on antibody-immobilized microfluidic device. Cell separation was achieved by tilting the surface 20 degrees and the increasing medium flow. Surface marker characteristics of the isolated cells in each fraction were analyzed using a cell-sorting system, and it was found that populations containing high density of CD34 were eluted in the delayed fractions. These results demonstrate that cells with a given surface marker density can be continuously separated using the cell rolling column. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010 [source] Three-dimensional incompressible flow calculations using the characteristic based split (CBS) schemeINTERNATIONAL JOURNAL FOR NUMERICAL METHODS IN FLUIDS, Issue 11 2004P. Nithiarasu Abstract In this paper, the characteristic based split scheme is employed for the solution of three-dimensional incompressible viscous flow problems on unstructured meshes. Many algorithm related issues are discussed. Fully explicit and semiimplicit forms of the scheme are explained and employed in the calculation of both isothermal and nonisothermal incompressible flows simulation. The extension of the scheme to porous medium flows is also demonstrated with relevant examples. Copyright © 2004 John Wiley & Sons, Ltd. [source] | |||||||||||||